pLV3-CMV-INPP5K-3×FLAG-EF1a-mC

pLV3-CMV-INPP5K-3×FLAG-EF1a-mCherry-Blast plasmid

PVT99955

pLV3-CMV-INPP5K-3×FLAG-EF1a-mCherry-Blast plasmid Information

Plasmid name: pLV3-CMV-INPP5K(human)-3×FLAG-EF1a-mCherry-Blast
Alias: NM_016532.4;MDCCAID; PPS; SKIP
Gene lenght: 1344bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Blast
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-INPP5K-3×FLAG-EF1a-mCherry-Blast plasmid Description

pLV3-CMV-INPP5K-3×FLAG-EF1a-mCherry-Blast It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-INPP5K-3×FLAG-EF1a-mCherry-Blast plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcGCCA CCatgagctc gcggaagctg agcgggccga aaggcaggag 2341 gctcagcata cacgtcgtga cttggaacgt ggcttcggca gcgccccctc tagatctcag 2401 tgacctgctt cagctgaaca accggaacct caatcttgac atatatgtta ttggtttgca 2461 ggaattgaac tctgggatca taagcctcct ttccgatgct gcctttaatg actcgtggag 2521 cagtttcctc atggatgtgc tttcccctct gagcttcatc aaggtctccc atgtccgtat 2581 gcaggggatc ctcttactgg tctttgccaa gtatcagcat ttgccctata tccagattct 2641 gtctactaaa tccaccccca ctggcctgtt tgggtactgg gggaacaaag gtggagtcaa 2701 catctgcctg aagctttatg gctactatgt cagcatcatc aactgccacc tgcctcccca 2761 catttccaac aattaccagc ggctggagca ctttgaccgg atcctggaga tgcagaattg 2821 tgaggggcga gacatcccaa acatcctgga ccacgacctc attatctggt ttggagacat 2881 gaactttcgg atcgaggact ttgggttgca ctttgttcgg gaatccatta aaaatcggtg 2941 ctacggtggc ctgtgggaga aggaccagct cagcattgcc aagaaacatg acccgctgct 3001 ccgggagttc caggagggcc gcctactctt cccgcccacc tacaagtttg ataggaactc 3061 caacgactat gacaccagtg agaaaaaacg caagcctgca tggaccgatc gcatcctgtg 3121 gaggctgaag cggcagccct gtgctggccc cgacactccc ataccgccgg cgtcacactt 3181 ctccttgtct ctgaggggct acagcagcca catgacgtac ggcatcagcg accacaagcc 3241 tgtctccggc acgttcgact tggagctgaa gccattggtg tctgctccgc tgatcgtcct 3301 gatgcccgag gacctgtgga ccgtggaaaa tgacatgatg gtcagctact cttcaacctc 3361 ggacttcccc agcagcccgt gggactggat tggactgtac aaggtggggc tgcgggacgt 3421 taatgactac gtgtcctatg cctgggtcgg ggacagcaag gtctcctgca gcgacaacct 3481 gaaccaggtt tacatcgaca tcagcaatat ccctaccact gaagatgagt ttctcctctg 3541 ttactacagc aacagtctgc gttctgtggt ggggataagc agacccttcc agatcccgcc 3601 tggctccttg agggaggacc cactgggtga agcacagcca cagatcggat ccGACTACAA 3661 AGACCATGAC GGTGATTATA AAGATCATGA CATCGACTAC AAGGATGACG ATGACAAGta 3721 gcggccgcGa aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc 3781 cacagtcccc gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg 3841 gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg 3901 gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc 3961 cgccagaaca cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc 4021 tacctgaggc cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc 4081 ctcctgaact gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg 4141 tccggcgctc ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct 4201 gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct 4261 gtgaccggcg cctacgctag acgccaccat ggtgagcaag ggcgaggagg ataacatggc 4321 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga 4381 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct 4441 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat 4501 gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc 4561 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac 4621 cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg 4681 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc 4741 ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa 4801 gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc 4861 cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga 4921 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat 4981 ggacgagctg tacaagggat ctcgcgaggg cagaggaagt cttctaacat gcggtgacgt 5041 ggaggagaat cccggccctA TGGCCAAGCC TTTGTCTCAA GAAGAATCCA CCCTCATTGA 5101 AAGAGCAACG GCTACAATCA ACAGCATCCC CATCTCTGAA GACTACAGCG TCGCCAGCGC 5161 AGCTCTCTCT AGCGACGGCC GCATCTTCAC TGGTGTCAAT GTATATCATT TTACTGGGGG 5221 ACCTTGTGCA GAACTCGTGG TGCTGGGCAC TGCTGCTGCT GCGGCAGCTG GCAACCTGAC 5281 TTGTATCGTC GCGATCGGAA ATGAGAACAG GGGCATCTTG AGCCCCTGCG GACGGTGCCG 5341 ACAGGTGCTT CTCGATCTGC ATCCTGGGAT CAAAGCCATA GTGAAGGACA GTGATGGACA 5401 GCCGACGGCA GTTGGGATTC GTGAATTGCT GCCCTCTGGT TATGTGTGGG AGGGCTAAGT 5461 CGACaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt 5521 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc 5581 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga 5641 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc 5701 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct 5761 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg 5821 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct 5881 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc 5941 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg 6001 tctccgcctt cgccctcaga cgagtcggat ctccctttgg ccgcctcccc gcctggtacc 6061 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg 6121 gggactggaa gggctaattc actcccaacg aaGataagat ctgctttttg cttgtactgg 6181 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact 6241 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg 6301 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag 6361 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga 6421 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 6481 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 6541 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc cagttccgcc 6601 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg 6661 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctaga cttttgcaga 6721 gacggcccaa attcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc 6781 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga 6841 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 6901 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 6961 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 7021 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 7081 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 7141 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 7201 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 7261 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 7321 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 7381 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 7441 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 7501 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 7561 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca 7621 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 7681 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 7741 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 7801 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 7861 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 7921 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 7981 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 8041 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 8101 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 8161 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 8221 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 8281 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 8341 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 8401 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 8461 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 8521 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 8581 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 8641 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 8701 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 8761 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 8821 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 8881 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat 8941 aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga 9001 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa 9061 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca 9121 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta 9181 aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg 9241 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg 9301 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt 9361 gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.