pBiT2.1-TK-SmBiT-KRAS plasmid

pBiT2.1-TK-SmBiT-KRAS plasmid

PVT104270

pBiT2.1-TK-SmBiT-KRAS plasmid Information

Plasmid name: pBiT2.1-TK-SmBiT-KRAS(human)
Alias: NM_033360.4;'C-K-RAS; C-K-RAS; c-Ki-ras; c-Ki-ras2;CFC2; K-Ras; K-Ras 2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B;KI-RAS; KRAS1; KRAS2; NS; NS3; OES; RALD; RASK2
Gene lenght: 570
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: HSV TK
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: HSV TK-F1
Reverse primer:
Copy number: High
Induction:

pBiT2.1-TK-SmBiT-KRAS plasmid Description

pBiT2.1-TK-SmBiT-KRAS is a plasmid suitable for protein expression in mammalian cells.

pBiT2.1-TK-SmBiT-KRAS plasmid Sequence

1 GGCCTAACTG GCCGGTACCT GAGTCTAAAT GAGTCTTCGG ACCTCGCGGG GGCCGCTTAA 61 GCGGTGGTTA GGGTTTGTCT GACGCGGGGG GAGGGGGAAG GAACGAAACA CTCTCATTCG 121 GAGGCGGCTC GGGGTTTGGT CTTGGTGGCC ACGGGCACGC AGAAGAGCGC CGCGATCCTC 181 TTAAGCACCC CCCCGCCCTC CGTGGAGGCG GGGGTTTGGT CGGCGGGTGG TAACTGGCGG 241 GCCGCTGACT CGGGCGGGTC GCGCGCCCCA GAGTGTGACC TTTTCGGTCT GCTCGCAGAC 301 CCCCGGGCGG CGCCGCCGCG GCGGCGACGG GCTCGCTGGG TCCTAGGCTC CATGGGGACC 361 GTATACGTGG ACAGGCTCTG GAGCATCCGC ACGACTGCGG TGATATTACC GGAGACCTTC 421 TGCGGGACGA GCCGGGTCAC GCGGCTGACG CGGAGCGTCC GTTGGGCGAC AAACACCAGG 481 ACGGGGCACA GGTACACTAT CTTGTCACCC GGAGGCGCGA GGGACTGCAG GAGCTTCAGG 541 GAGTGGCGCA GCTGCTTCAT CCCCGTGGCC CGTTGCTCGC GTTTGCTGGC GGTGTCCCCG 601 GAAGAAATAT ATTTGCATGT CTTTAGTTCT ATGATGACAC AAACCCCGCC CAGCGTCTTG 661 TCATTGGCGA AGTCGAACAC GCAGATGCAG TCGGGGCGGC GCGGTCCCAG GTCCACTTCG 721 CATATTAAGG TGACGCGTGT GGCCTCGAAC ACCGAGCGAC CCTGCAGCGA CCCGCTTAAA 781 AGCTTGGCAA TCCGGTACTG TTGGTAAAGC CACCATGGTG ACCGGCTACC GGCTGTTCGA 841 GGAGATTCTC GGGAGTTCCG GTGGTGGCGG GAGCGGAGGT GGAGGCTCGa tgactgaata 901 taaacttgtg gtagttggag ctggtggcgt aggcaagagt gccttgacga tacagctaat 961 tcagaatcat tttgtggacg aatatgatcc aacaatagag gattcctaca ggaagcaagt 1021 agtaattgat ggagaaacct gtctcttgga tattctcgac acagcaggtc aagaggagta 1081 cagtgcaatg agggaccagt acatgaggac tggggagggc tttctttgtg tatttgccat 1141 aaataatact aaatcatttg aagatattca ccattataga gaacaaatta aaagagttaa 1201 ggactctgaa gatgtaccta tggtcctagt aggaaataaa tgtgatttgc cttctagaac 1261 agtagacaca aaacaggctc aggacttagc aagaagttat ggaattcctt ttattgaaac 1321 atcagcaaag acaagacaga gagtggagga tgctttttat acattggtga gagagatccg 1381 acaatacaga ttgaaaaaaa tcagcaaaga agaaaagact cctggctgtg tgaaaattaa 1441 aaaatgcatt ataatgtaaG AATTCAGTCT AAGCTAGCAG ATCTTCTAGA GTCGGGGCGG 1501 CCGGCCGCTT CGAGCAGACA TGATAAGATA CATTGATGAG TTTGGACAAA CCACAACTAG 1561 AATGCAGTGA AAAAAATGCT TTATTTGTGA AATTTGTGAT GCTATTGCTT TATTTGTAAC 1621 CATTATAAGC TGCAATAAAC AAGTTAACAA CAACAATTGC ATTCATTTTA TGTTTCAGGT 1681 TCAGGGGGAG GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTAAAAT 1741 CGATAAGGAT CCGTCGACCG ATGCCCTTGA GAGCCTTCAA CCCAGTCAGC TCCTTCCGGT 1801 GGGCGCGGGG CATGACTATC GTCGCCGCAC TTATGACTGT CTTCTTTATC ATGCAACTCG 1861 TAGGACAGGT GCCGGCAGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC 1921 GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA 1981 TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT 2041 AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA 2101 AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT 2161 CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG 2221 TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC 2281 AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC 2341 GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA 2401 TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT 2461 ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC 2521 TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA 2581 CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA 2641 AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA 2701 AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT 2761 TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC 2821 AGCGGCCGCA AATGCTAAAC CACTGCAGTG GTTACCAGTG CTTGATCAGT GAGGCACCGA 2881 TCTCAGCGAT CTGCCTATTT CGTTCGTCCA TAGTGGCCTG ACTCCCCGTC GTGTAGATCA 2941 CTACGATTCG TGAGGGCTTA CCATCAGGCC CCAGCGCAGC AATGATGCCG CGAGAGCCGC 3001 GTTCACCGGC CCCCGATTTG TCAGCAATGA ACCAGCCAGC AGGGAGGGCC GAGCGAAGAA 3061 GTGGTCCTGC TACTTTGTCC GCCTCCATCC AGTCTATGAG CTGCTGTCGT GATGCTAGAG 3121 TAAGAAGTTC GCCAGTGAGT AGTTTCCGAA GAGTTGTGGC CATTGCTACT GGCATCGTGG 3181 TATCACGCTC GTCGTTCGGT ATGGCTTCGT TCAACTCTGG TTCCCAGCGG TCAAGCCGGG 3241 TCACATGATC ACCCATATTA TGAAGAAATG CAGTCAGCTC CTTAGGGCCT CCGATCGTTG 3301 TCAGAAGTAA GTTGGCCGCG GTGTTGTCGC TCATGGTAAT GGCAGCACTA CACAATTCTC 3361 TTACCGTCAT GCCATCCGTA AGATGCTTTT CCGTGACCGG CGAGTACTCA ACCAAGTCGT 3421 TTTGTGAGTA GTGTATACGG CGACCAAGCT GCTCTTGCCC GGCGTCTATA CGGGACAACA 3481 CCGCGCCACA TAGCAGTACT TTGAAAGTGC TCATCATCGG GAATCGTTCT TCGGGGCGGA 3541 AAGACTCAAG GATCTTGCCG CTATTGAGAT CCAGTTCGAT ATAGCCCACT CTTGCACCCA 3601 GTTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCGGG GTGTGCAAAA ACAGGCAAGC 3661 AAAATGCCGC AAAGAAGGGA ATGAGTGCGA CACGAAAATG TTGGATGCTC ATACTCGTCC 3721 TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTACTAGTA CGTCTCTCAA GGATAAGTAA 3781 GTAATATTAA GGTACGGGAG GTATTGGACA GGCCGCAATA AAATATCTTT ATTTTCATTA 3841 CATCTGTGTG TTGGTTTTTT GTGTGAATCG ATAGTACTAA CATACGCTCT CCATCAAAAC 3901 AAAACGAAAC AAAACAAACT AGCAAAATAG GCTGTCCCCA GTGCAAGTGC AGGTGCCAGA 3961 ACATTTCTCT

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.