pLV3-U6-VDAC1-shRNA1-EF1a-Puro

pLV3-U6-VDAC1-shRNA1-EF1a-Puro plasmid

PVT99020

pLV3-U6-VDAC1-shRNA1-EF1a-Puro plasmid Information

Plasmid name: pLV3-U6-VDAC1(human)-shRNA1-EF1a-Puro
Alias: gcttggtctaggactggaatt
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Interference silence
Fragment type: shRNA
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pLV3-U6-VDAC1-shRNA1-EF1a-Puro plasmid Description

pLV3-U6-VDAC1-shRNA1-EF1a-Puro This is a plasmid suitable for interference silencing in mammalian cells, and it can also be used for lentivirus packaging.

pLV3-U6-VDAC1-shRNA1-EF1a-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tCGGGTTTAT TACAGGGACA GCAGAGATCC AGTTTATCGA TCTGGGCAGG AAGAGGGCCT 1981 ATTTCCCATG ATTCCTTCAT ATTTGCATAT ACGATACAAG GCTGTTAGAG AGATAATTAG 2041 AATTAATTTG ACTGTAAACA CAAAGATATT AGTACAAAAT ACGTGACGTA GAAAGTAATA 2101 ATTTCTTGGG TAGTTTGCAG TTTTAAAATT ATGTTTTAAA ATGGACTATC ATATGCTTAC 2161 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGGATC 2221 CGgcttggtc taggactgga attCTCGAGa attccagtcc tagaccaagc TTTTTTGAAT 2281 TCgaaggatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt 2341 ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg 2401 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga 2461 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag 2521 aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg 2581 aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg 2641 aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc 2701 gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc 2761 tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc 2821 ggcgcctacg ctagatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg 2881 tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca 2941 ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc 3001 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct 3061 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg 3121 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc 3181 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg 3241 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg 3301 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct 3361 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca 3421 agcccggtgc ctgagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg 3481 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt 3541 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc 3601 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt 3661 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga 3721 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct 3781 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat 3841 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct 3901 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc 3961 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg 4021 cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac 4081 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaaa ataagatctg 4141 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc 4201 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg 4261 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg 4321 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca 4381 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa 4441 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 4501 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa 4561 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag 4621 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag 4681 gcctagactt ttgcagagac ggcccaaatt cgtaatcatg gtcatagctg tttcctgtgt 4741 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 4801 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt 4861 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 4921 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 4981 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 5041 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 5101 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 5161 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 5221 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 5281 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 5341 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 5401 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 5461 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 5521 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 5581 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 5641 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 5701 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 5761 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 5821 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 5881 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 5941 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 6001 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 6061 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 6121 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 6181 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 6241 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 6301 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 6361 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 6421 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 6481 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 6541 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 6601 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 6661 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 6721 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 6781 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 6841 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga 6901 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg 6961 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg 7021 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct 7081 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa 7141 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc 7201 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag 7261 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt 7321 gtaaaacgac ggccagtgcc aagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.