pCMV-SP-EXOSC10-TM-P2A-mCherry

pCMV-SP-EXOSC10-TM-P2A-mCherry-Neo plasmid

PVT98759

pCMV-SP-EXOSC10-TM-P2A-mCherry-Neo plasmid Information

Plasmid name: pCMV-SP-EXOSC10(human)-TM-P2A-mCherry-Neo
Alias: NM_001001998.3;p2; p3; p4; PM-Scl; PM/Scl-100; PMSCL;PMSCL2; RRP6; Rrp6p
Gene lenght: 2655bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-SP-EXOSC10-TM-P2A-mCherry-Neo plasmid Description

pCMV-SP-EXOSC10-TM-P2A-mCherry-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-SP-EXOSC10-TM-P2A-mCherry-Neo plasmid Sequence

1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg 661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg 721 actctagagc tagcgtttaa acttaagctt gAGCCACCAT GAGGGCCTGG ATCTTCTTTC 781 TCCTTTGCCT GGCCGGGAGG GCCTTGGCAC TGCCCGTCat ggcgccaccc agtacccggg 841 agcccagggt cctgtcggcg accagcgcaa ccaaatccga cggagagatg gtgctgccag 901 gcttcccgga cgccgacagc tttgtgaagt ttgctcttgg gtccgtggtg gcagtcacca 961 aggcatctgg gggcctacca cagtttggcg atgagtatga tttttaccga agttttcctg 1021 gcttccaagc attttgcgaa acacagggag acaggttgct tcagtgcatg agcagagtaa 1081 tgcagtacca tgggtgtcgc agcaacatta aggatcgaag taaagtgact gagctggaag 1141 acaagtttga tttactagtt gatgccaatg atgtaattct ggagagagtg ggtattttac 1201 tggatgaagc ctcaggtgta aacaagaatc aacagcctgt cctccctgcc ggcttgcagg 1261 tccccaaaac ggtagtgtcc agctggaacc gtaaggcagc agaatatggc aaaaaagcaa 1321 aatctgaaac tttccggctg cttcatgcaa aaaatatcat ccgacctcag ctcaagtttc 1381 gagagaagat tgacaattcc aacacaccat ttcttcctaa aatcttcatc aaacccaatg 1441 ctcagaaacc tctccctcaa gctctctcta aggaaaggcg ggaacgccca caggatcgtc 1501 ctgaggactt ggacgtcccc cctgcactgg ctgatttcat ccatcagcag agaacccagc 1561 aggttgagca agacatgttt gcacatcctt atcaatatga actaaatcac tttaccccag 1621 cagatgcagt gcttcaaaag ccacaacccc agttatacag acctatagaa gagacaccat 1681 gccatttcat atcctccctg gatgaactcg tggaactcaa cgaaaagctc ttgaattgtc 1741 aggaatttgc agttgacttg gagcaccact cttacaggag cttcctggga ctgacctgcc 1801 tgatgcaaat ttctactcgg acggaagact tcatcattga caccctcgag cttcgaagtg 1861 acatgtacat tctcaatgag agcctcacag acccagccat cgttaaggtc tttcatggtg 1921 ctgattcaga catagaatgg ctacagaaag actttgggtt gtatgtagta aacatgtttg 1981 atactcatca ggcagcacgc cttcttaacc tgggcaggca ctcactcgat catctcctga 2041 aactctactg caacgtggac tcaaacaagc aatatcagct ggctgattgg agaatacgcc 2101 ctctgcccga ggagatgctc agctacgccc gggatgacac ccattacctg ctatatatct 2161 atgacaaaat gaggctggag atgtgggagc gcggcaacgg gcagccggtg cagctgcagg 2221 tggtgtggca acggagcagg gacatctgcc tcaagaaatt catcaaacct atcttcacgg 2281 atgagtccta ccttgaactc tataggaagc agaagaagca ccttaacaca cagcagttga 2341 cagcctttca gctgctgttt gcctggaggg ataaaacagc tcgcagggaa gatgaaagtt 2401 acggatatgt actgccaaac cacatgatgc tgaaaatagc tgaagaactg cctaaggaac 2461 ctcagggcat catagcttgc tgcaacccag taccgcccct tgtgcggcag cagatcaacg 2521 aaatgcacct tttaatccag caggcccgag agatgcccct gctcaagtct gaagttgcag 2581 ccggagtgaa gaagagcgga ccgctgccca gtgctgagag attggagaat gttctctttg 2641 gacctcacga ctgctcccat gcccctccgg atggctatcc aatcatccca accagtggat 2701 ctgtgccagt tcagaagcag gcgagcctct tccctgatga aaaagaagat aacttgctgg 2761 gtaccacatg cctgattgcc acagctgtca tcacgttatt taatgaacct agtgctgaag 2821 acagtaaaaa gggtccattg acagttgcac agaaaaaagc ccagaacatc atggagtcct 2881 ttgaaaatcc atttaggatg tttctgccct cactgggaca ccgtgctccc gtctctcagg 2941 cagcgaagtt cgatccatca accaaaatct atgaaatcag caaccgctgg aagctggccc 3001 aggtacaagt acaaaaagac tctaaagaag ctgtcaagaa gaaggcagct gagcaaacag 3061 ctgcccggga acaggcaaag gaggcgtgca aagctgcagc agaacaggcc atctccgtcc 3121 gacagcaggt cgtgctagaa aatgctgcaa agaagagaga gcgagcaaca agcgacccaa 3181 ggaccacaga acagaaacaa gagaagaaac gactcaaaat ttccaagaag ccaaaggacc 3241 cagagccacc agaaaaagag tttacgcctt acgactacag ccagtcagac ttcaaggctt 3301 ttgctggaaa cagcaaatcc aaagtttctt ctcagtttga tccaaataaa cagaccccgt 3361 ctggcaagaa atgcattgca gccaaaaaaa ttaaacagtc ggtgggaaac aaaagcatgt 3421 cctttccaac tggaaagtca gacagaggct tcaggtacaa ctggccacag agaGGCTcCA 3481 CCAGCGGCAG CGGCAAGCCA GGCTCCGGCG aAGGCAGCAC CAAAGGCGAT CTGAATgctg 3541 tgggccagga cacgcaggag gtcatcgtgg tgccacactc cttgcccttt aaggtggtgg 3601 tgatctcagc catcctggcc ctggtggtgc tcaccatcat ctcccttatc atcctcatca 3661 tgctttggca gaagaagcca cgtATAGGGA TCCGTCTCGA GTCTAGAGGG CCCTTCGGGG 3721 GTGGAGGCTC Tggaagcgga gctactaact tcagcctgct gaagcaggct ggcgacgtgg 3781 aggagaaccc tggacctatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg 3841 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg 3901 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca 3961 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca 4021 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg 4081 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg 4141 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc 4201 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga 4261 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg 4321 gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc 4381 ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca 4441 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt 4501 acaagtaact cgagtctaga gggcccctta agggttcgat ccctaccggt tagtaatgag 4561 tttgatatct cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc 4621 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg 4681 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc 4741 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg 4801 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg 4861 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga 4921 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct 4981 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg 5041 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc 5101 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc 5161 cgcctggaaa cgggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg 5221 cgctatgacg gcaataaaaa gacagaataa aacgcacggg tgttgggtcg tttgttcata 5281 aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg 5341 gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc 5401 cagggctcgc agccaacgtc ggggcggcag gccctgccat agcagatctg cgcagctggg 5461 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg 5521 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct 5581 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cggggcatcc 5641 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg 5701 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt 5761 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg 5821 tctattcttt tgatttataa gggattttgg ggatttcggc ctattggtta aaaaatgagc 5881 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg 5941 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc 6001 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct 6061 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc 6121 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga 6181 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg 6241 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg 6301 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg 6361 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc 6421 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg 6481 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt 6541 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg 6601 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat 6661 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca 6721 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca 6781 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa 6841 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa 6901 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc 6961 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga 7021 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc 7081 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcgc gaaatgaccg 7141 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa 7201 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc 7261 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat 7321 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg 7381 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga 7441 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 7501 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct 7561 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 7621 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 7681 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 7741 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 7801 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 7861 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 7921 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 7981 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 8041 tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 8101 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 8161 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 8221 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 8281 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 8341 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 8401 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 8461 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 8521 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 8581 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 8641 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 8701 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 8761 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 8821 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 8881 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 8941 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 9001 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 9061 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 9121 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 9181 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 9241 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 9301 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 9361 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 9421 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 9481 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 9541 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 9601 tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtcga ctctcagtac 9661 aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt 9721 cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg 9781 catgaagaat ctgcttagg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.