pCMV-SP-DPYSL5-TM-P2A-mCherry-

pCMV-SP-DPYSL5-TM-P2A-mCherry-Neo plasmid

PVT98751

pCMV-SP-DPYSL5-TM-P2A-mCherry-Neo plasmid Information

Plasmid name: pCMV-SP-DPYSL5(human)-TM-P2A-mCherry-Neo
Alias: NM_020134.4;CRAM; CRMP-5; CRMP5; CV2; RTSC4; Ulip6
Gene lenght: 1692bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-SP-DPYSL5-TM-P2A-mCherry-Neo plasmid Description

pCMV-SP-DPYSL5-TM-P2A-mCherry-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-SP-DPYSL5-TM-P2A-mCherry-Neo plasmid Sequence

1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg 661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg 721 actctagagc tagcgtttaa acttaagctt gAGCCACCAT GAGGGCCTGG ATCTTCTTTC 781 TCCTTTGCCT GGCCGGGAGG GCCTTGGCAC TGCCCGTCat gcttgccaac tcagccagcg 841 tgaggatcct catcaaggga ggcaaggtgg tgaacgatga ctgcacccac gaggctgacg 901 tctacatcga gaatggcatc atccagcagg tgggccgcga gctcatgatc cctggcgggg 961 ccaaggtgat tgatgccaca ggaaaactgg tgatccctgg tggcatcgac accagcaccc 1021 acttccacca gaccttcatg aatgccacgt gcgtggacga cttctaccat gggaccaagg 1081 cagcactcgt cggaggcacc accatgatca tcggccacgt cctgcccgac aaggagacct 1141 cccttgtgga cgcttatgag aagtgccgag gtctggccga ccccaaggtc tgctgtgatt 1201 acgccctcca cgtggggatc acctggtggg cacccaaggt gaaagcagaa atggagacac 1261 tggtgaggga gaagggtgtc aactcgttcc agatgttcat gacctacaag gacctgtaca 1321 tgcttcgaga cagtgagctg taccaagtgt tgcacgcttg caaggacatt ggggcaatcg 1381 cccgcgtcca tgctgaaaat ggggagcttg tggccgaggg tgctaaggag gcactggatt 1441 tggggatcac aggcccagaa ggaatcgaga tcagccgtcc agaggagctg gaagctgaag 1501 ccactcatcg tgttatcacc attgcaaaca ggactcactg tccaatctac ctggtcaacg 1561 tgtccagtat ctcggctggt gacgttatcg cagctgctaa gatgcaaggg aaggttgtgc 1621 tggcggagac caccactgca catgccacgc tgacaggctt acactactac caccaggact 1681 ggtcccacgc ggctgcctat gtcacggtgc ctcccctgag actggacacc aacacctcaa 1741 cctacctcat gagcctgctg gccaatgaca ctctgaacat cgtggcatca gatcaccggc 1801 ctttcaccac aaagcagaaa gctatgggca aggaagactt caccaagatc ccacatggag 1861 tgagtggcgt gcaggaccgc atgagcgtca tctgggagag aggagtggtt ggaggaaaga 1921 tggatgagaa ccgttttgtg gccgttacca gttccaacgc agctaagctt ctgaacctgt 1981 atccccgcaa gggccgcatt attcccggag ccgatgctga tgtggtggtg tgggacccag 2041 aagccacaaa gaccatctca gccagcacgc aggtccaggg aggagacttc aacctgtatg 2101 agaacatgcg ctgccacggc gtgccactgg tcaccatcag ccgggggcgc gtcgtgtatg 2161 agaacggcgt cttcatgtgc gccgagggca ccggcaagtt ctgtcccctg aggtccttcc 2221 cagacactgt ctacaagaag ctggtccaga gagagaagac tttaaaggtt agaggagtgg 2281 accgcactcc ctacctgggg gatgtcgctg ttgtcgtgca ccctgggaaa aaagagatgg 2341 gaaccccact cgcagacact cctacccggc ccgtcacccg gcatgggggc atgagggacc 2401 ttcacgaatc cagcttcagc ctctctggct ctcagatcga tgaccatgtt ccaaagcgag 2461 cttcagctcg gatcctcgct cctcccggag gcaggtcgag tggcatttgg GGCTcCACCA 2521 GCGGCAGCGG CAAGCCAGGC TCCGGCGaAG GCAGCACCAA AGGCGATCTG AATgctgtgg 2581 gccaggacac gcaggaggtc atcgtggtgc cacactcctt gccctttaag gtggtggtga 2641 tctcagccat cctggccctg gtggtgctca ccatcatctc ccttatcatc ctcatcatgc 2701 tttggcagaa gaagccacgt ATAGGGATCC GTCTCGAGTC TAGAGGGCCC TTCGGGGGTG 2761 GAGGCTCTgg aagcggagct actaacttca gcctgctgaa gcaggctggc gacgtggagg 2821 agaaccctgg acctatggtg agcaagggcg aggaggataa catggccatc atcaaggagt 2881 tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg 2941 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg 3001 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg 3061 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct 3121 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact 3181 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct 3241 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt 3301 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg 3361 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg 3421 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg 3481 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca 3541 agtaactcga gtctagaggg ccccttaagg gttcgatccc taccggttag taatgagttt 3601 gatatctcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta 3661 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta 3721 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt 3781 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg 3841 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt 3901 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag 3961 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc 4021 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc 4081 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc 4141 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc 4201 ctggaaacgg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc 4261 tatgacggca ataaaaagac agaataaaac gcacgggtgt tgggtcgttt gttcataaac 4321 gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc 4381 aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag 4441 ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc agatctgcgc agctggggct 4501 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta 4561 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc 4621 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg ggcatccctt 4681 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg 4741 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca 4801 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct 4861 attcttttga tttataaggg attttgggga tttcggccta ttggttaaaa aatgagctga 4921 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa 4981 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac 5041 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa 5101 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag 5161 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc 5221 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt 5281 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg 5341 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt 5401 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt 5461 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc 5521 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc 5581 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga 5641 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat 5701 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca 5761 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga 5821 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc 5881 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat 5941 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga 6001 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg 6061 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt 6121 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgcgaa atgaccgacc 6181 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt 6241 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca 6301 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa 6361 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt 6421 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct 6481 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac 6541 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac 6601 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc 6661 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg 6721 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 6781 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 6841 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 6901 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 6961 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 7021 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 7081 cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 7141 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 7201 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 7261 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 7321 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 7381 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 7441 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 7501 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 7561 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 7621 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 7681 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 7741 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 7801 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 7861 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 7921 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 7981 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 8041 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 8101 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 8161 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 8221 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 8281 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 8341 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 8401 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 8461 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 8521 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 8581 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 8641 cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtcgactc tcagtacaat 8701 ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc 8761 tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat 8821 gaagaatctg cttagg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.