pLV3-CMV-3×HA-STAT1-EF1a-CopGF

pLV3-CMV-3×HA-STAT1-EF1a-CopGFP-Puro plasmid

PVT98639

pLV3-CMV-3×HA-STAT1-EF1a-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-3×HA-STAT1(human)-EF1a-CopGFP-Puro
Alias: NM_001384880.1;CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91
Gene lenght: 2193bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-3×HA-STAT1-EF1a-CopGFP-Puro plasmid Description

pLV3-CMV-3×HA-STAT1-EF1a-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-3×HA-STAT1-EF1a-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc 2281 caccatgTAC CCATACGACG TACCTGATTA TGCAACTTAT CCTTATGATG TACCTGATTA 2341 TGCTAGCTAT CCTTATGATG TCCCAGACTA CGCAgaattc atgtctcagt ggtacgaact 2401 tcagcagctt gactcaaaat tcctggagca ggttcaccag ctttatgatg acagttttcc 2461 catggaaatc agacagtacc tggcacagtg gttagaaaag caagactggg agcacgctgc 2521 caatgatgtt tcatttgcca ccatccgttt tcatgacctc ctgtcacagc tggatgatca 2581 atatagtcgc ttttctttgg agaataactt cttgctacag cataacataa ggaaaagcaa 2641 gcgtaatctt caggataatt ttcaggaaga cccaatccag atgtctatga tcatttacag 2701 ctgtctgaag gaagaaagga aaattctgga aaacgcccag agatttaatc aggctcagtc 2761 ggggaatatt cagagcacag tgatgttaga caaacagaaa gagcttgaca gtaaagtcag 2821 aaatgtgaag gacaaggtta tgtgtataga gcatgaaatc aagagcctgg aagatttaca 2881 agatgaatat gacttcaaat gcaaaacctt gcagaacaga gaacacgaga ccaatggtgt 2941 ggcaaagagt gatcagaaac aagaacagct gttactcaag aagatgtatt taatgcttga 3001 caataagaga aaggaagtag ttcacaaaat aatagagttg ctgaatgtca ctgaacttac 3061 ccagaatgcc ctgattaatg atgaactagt ggagtggaag cggagacagc agagcgcctg 3121 tattgggggg ccgcccaatg cttgcttgga tcagctgcag aactggttca ctatagttgc 3181 ggagagtctg cagcaagttc ggcagcagct taaaaagttg gaggaattgg aacagaaata 3241 cacctacgaa catgacccta tcacaaaaaa caaacaagtg ttatgggacc gcaccttcag 3301 tcttttccag cagctcattc agagctcgtt tgtggtggaa agacagccct gcatgccaac 3361 gcaccctcag aggccgctgg tcttgaagac aggggtccag ttcactgtga agttgagaga 3421 tgtgaatgag agaaatacag taaaaggatt taggaagttc aacattttgg gcacgcacac 3481 aaaagtgatg aacatggagg agtccaccaa tggcagtctg gcggctgaat ttcggcacct 3541 gcaattgaaa gaacagaaaa atgctggcac cagaacgaat gagggtcctc tcatcgttac 3601 tgaagagctt cactccctta gttttgaaac ccaattgtgc cagcctggtt tggtaattga 3661 cctcgagacg acctctctgc ccgttgtggt gatctccaac gtcagccagc tcccgagcgg 3721 ttgggcctcc atcctttggt acaacatgct ggtggcggaa cccaggaatc tgtccttctt 3781 cctgactcca ccatgtgcac gatgggctca gctttcagaa gtgctgagtt ggcagttttc 3841 ttctgtcacc aaaagaggtc tcaatgtgga ccagctgaac atgttgggag agaagcttct 3901 tggtcctaac gccagccccg atggtctcat tccgtggacg aggttttgta aggaaaatat 3961 aaatgataaa aattttccct tctggctttg gattgaaagc atcctagaac tcattaaaaa 4021 acacctgctc cctctctgga atgatgggtg catcatgggc ttcatcagca aggagcgaga 4081 gcgtgccctg ttgaaggacc agcagccggg gaccttcctg ctgcggttca gtgagagctc 4141 ccgggaaggg gccatcacat tcacatgggt ggagcggtcc cagaacggag gcgaacctga 4201 cttccatgcg gttgaaccct acacgaagaa agaactttct gctgttactt tccctgacat 4261 cattcgcaat tacaaagtca tggctgctga gaatattcct gagaatcccc tgaagtatct 4321 gtatccaaat attgacaaag accatgcctt tggaaagtat tactccaggc caaaggaagc 4381 accagagcca atggaacttg atggccctaa aggaactgga tatatcaaga ctgagttgat 4441 ttctgtgtct gaagttcacc cttctagact tcagaccaca gacaacctgc tccccatgtc 4501 tcctgaggag tttgacgagg tgtctcggat agtgggctct gtagaattcg acagtatgat 4561 gaacacagta tagggatccg cggccgcgaa ggatctgcga tcgctccggt gcccgtcagt 4621 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa 4681 cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc 4741 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc 4801 tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct cgcatctctc 4861 cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt cgcgttctgc 4921 cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca 4981 ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc agccggctct 5041 ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt tctgttctgc 5101 gccgttacag atccaagctg tgaccggcgc ctacgctaga cgccaccatg gagagcgacg 5161 agagcggcct gcccgccatg gagatcgagt gccgcatcac cggcaccctg aacggcgtgg 5221 agttcgagct ggtgggcggc ggagagggca cccccaagca gggccgcatg accaacaaga 5281 tgaagagcac caaaggcgcc ctgaccttca gcccctacct gctgagccac gtgatgggct 5341 acggcttcta ccacttcggc acctacccca gcggctacga gaaccccttc ctgcacgcca 5401 tcaacaacgg cggctacacc aacacccgca tcgagaagta cgaggacggc ggcgtgctgc 5461 acgtgagctt cagctaccgc tacgaggccg gccgcgtgat cggcgacttc aaggtggtgg 5521 gcaccggctt ccccgaggac agcgtgatct tcaccgacaa gatcatccgc agcaacgcca 5581 ccgtggagca cctgcacccc atgggcgata acgtgctggt gggcagcttc gcccgcacct 5641 tcagcctgcg cgacggcggc tactacagct tcgtggtgga cagccacatg cacttcaaga 5701 gcgccatcca ccccagcatc ctgcagaacg ggggccccat gttcgccttc cgccgcgtgg 5761 aggagctgca cagcaacacc gagctgggca tcgtggagta ccagcacgcc ttcaagaccc 5821 ccatcgcctt cgccagatcc cgcgctcagt cgtccaattc tgccgtggac ggcaccgccg 5881 gacccggctc caccggatct cgcgagggca gaggaagtct tctaacatgc ggtgacgtgg 5941 aggagaatcc cggccctatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg 6001 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc 6061 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca 6121 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg 6181 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca 6241 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc 6301 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc 6361 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg 6421 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg 6481 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc 6541 gcaagcccgg tgcctgaaat caacctctgg attacaaaat ttgtgaaaga ttgactggta 6601 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc 6661 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt 6721 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg 6781 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt 6841 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct 6901 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt 6961 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct 7021 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc 7081 ggcctcttcc gcgtctccgc cttcgccctc agacgagtcg gatctccctt tggccgcctc 7141 cccgcctggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt 7201 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaaaataa gatctgcttt 7261 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac 7321 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg 7381 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga 7441 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga 7501 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa 7561 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt 7621 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc 7681 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc 7741 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct 7801 agacttttgc agagacggcc caaattcgta atcatggtca tagctgtttc ctgtgtgaaa 7861 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 7921 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 7981 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 8041 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 8101 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 8161 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 8221 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 8281 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 8341 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 8401 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 8461 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 8521 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 8581 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 8641 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc 8701 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 8761 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 8821 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 8881 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 8941 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 9001 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 9061 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 9121 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 9181 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 9241 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 9301 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 9361 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 9421 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 9481 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 9541 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 9601 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 9661 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 9721 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 9781 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 9841 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 9901 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 9961 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt 10021 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg 10081 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc 10141 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct 10201 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc 10261 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa 10321 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg 10381 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa 10441 aacgacggcc agtgccaagc tg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.