pLV3-CMV-3×HA-HEXIM1-EF1a-CopG

pLV3-CMV-3×HA-HEXIM1-EF1a-CopGFP-Puro plasmid

PVT94831

pLV3-CMV-3×HA-HEXIM1-EF1a-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-3×HA-HEXIM1(human)-EF1a-CopGFP-Puro
Alias: NM_006460.3;CLP1; EDG1; HIS1; MAQ1
Gene lenght: 1080bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-3×HA-HEXIM1-EF1a-CopGFP-Puro plasmid Description

pLV3-CMV-3×HA-HEXIM1-EF1a-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-3×HA-HEXIM1-EF1a-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc 2281 caccatgTAC CCATACGACG TACCTGATTA TGCAACTTAT CCTTATGATG TACCTGATTA 2341 TGCTAGCTAT CCTTATGATG TCCCAGACTA CGCAgaattc atggccgagc cattcttgtc 2401 agaatatcaa caccagcctc aaactagcaa ctgtacaggt gctgctgctg tccaggaaga 2461 gctgaaccct gagcgccccc caggcgcgga ggagcgggtg cccgaggagg acagtaggtg 2521 gcaatcgaga gcgttccccc agttgggtgg ccgtccgggg ccggaggggg aagggagcct 2581 ggaatcccaa ccacctccct tgcagaccca ggcctgtcca gaatctagct gcctgagaga 2641 gggcgagaag ggccagaatg gggacgactc gtccgctggc ggcgacttcc cgccgccggc 2701 agaagtggaa ccgacgcccg aggccgagct gctcgcccag ccttgtcatg actccgaggc 2761 cagtaagttg ggggctcctg ccgcaggggg cgaagaggag tggggacagc agcagagaca 2821 gctggggaag aaaaaacata ggagacgccc gtccaagaag aagcggcatt ggaaaccgta 2881 ctacaagctg acctgggaag agaagaaaaa gttcgacgag aaacagagcc ttcgagcttc 2941 aaggatccga gccgagatgt tcgccaaggg ccagccggtc gcgccctata acaccacgca 3001 gttcctcatg gatgatcacg accaggagga gccggatctc aaaaccggcc tgtactccaa 3061 gcgggccgcc gccaaatccg acgacaccag cgatgacgac ttcatggaag aagggggtga 3121 ggaggatggg ggcagcgatg ggatgggagg ggacggcagc gagtttctgc agcgggactt 3181 ctcggagacg tacgagcggt accacacgga gagcctgcag aacatgagca agcaggagct 3241 catcaaggag tacctggaac tggagaagtg cctctcgcgc atggaggacg agaacaaccg 3301 gctgcggctg gagagcaagc ggctgggtgg cgacgacgcg cgtgtgcggg agctggagct 3361 ggagctggac cggctgcgcg ccgagaacct ccagctgctg accgagaacg aactgcaccg 3421 gcagcaggag cgagcgccgc tttccaagtt tggagactag ggatccgcgg ccgcgaagga 3481 tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga 3541 agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact 3601 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata 3661 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc 3721 tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc 3781 atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt 3841 ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt 3901 ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct 3961 acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta 4021 cgctagacgc caccatggag agcgacgaga gcggcctgcc cgccatggag atcgagtgcc 4081 gcatcaccgg caccctgaac ggcgtggagt tcgagctggt gggcggcgga gagggcaccc 4141 ccaagcaggg ccgcatgacc aacaagatga agagcaccaa aggcgccctg accttcagcc 4201 cctacctgct gagccacgtg atgggctacg gcttctacca cttcggcacc taccccagcg 4261 gctacgagaa ccccttcctg cacgccatca acaacggcgg ctacaccaac acccgcatcg 4321 agaagtacga ggacggcggc gtgctgcacg tgagcttcag ctaccgctac gaggccggcc 4381 gcgtgatcgg cgacttcaag gtggtgggca ccggcttccc cgaggacagc gtgatcttca 4441 ccgacaagat catccgcagc aacgccaccg tggagcacct gcaccccatg ggcgataacg 4501 tgctggtggg cagcttcgcc cgcaccttca gcctgcgcga cggcggctac tacagcttcg 4561 tggtggacag ccacatgcac ttcaagagcg ccatccaccc cagcatcctg cagaacgggg 4621 gccccatgtt cgccttccgc cgcgtggagg agctgcacag caacaccgag ctgggcatcg 4681 tggagtacca gcacgccttc aagaccccca tcgccttcgc cagatcccgc gctcagtcgt 4741 ccaattctgc cgtggacggc accgccggac ccggctccac cggatctcgc gagggcagag 4801 gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctatgacc gagtacaagc 4861 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg 4921 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg 4981 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg 5041 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg 5101 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc 5161 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg 5221 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc 5281 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc 5341 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg 5401 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaaatcaa cctctggatt 5461 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg 5521 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct 5581 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc 5641 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca 5701 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac 5761 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt 5821 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct 5881 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc 5941 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tctccgcctt cgccctcaga 6001 cgagtcggat ctccctttgg ccgcctcccc gcctggtacc tttaagacca atgacttaca 6061 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc 6121 actcccaacg aaaataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga 6181 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct 6241 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat 6301 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta 6361 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta 6421 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 6481 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 6541 ggctctagct atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg 6601 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa 6661 gtagtgagga ggcttttttg gaggcctaga cttttgcaga gacggcccaa attcgtaatc 6721 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg 6781 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat 6841 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg 6901 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct 6961 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 7021 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 7081 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 7141 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 7201 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 7261 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 7321 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 7381 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 7441 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 7501 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 7561 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 7621 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 7681 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 7741 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 7801 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 7861 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 7921 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 7981 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 8041 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 8101 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 8161 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 8221 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 8281 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 8341 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 8401 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 8461 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 8521 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 8581 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 8641 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 8701 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 8761 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 8821 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt 8881 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt 8941 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 9001 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 9061 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag 9121 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag 9181 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc 9241 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc 9301 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.