pCMV-Atg2a-3×Myc-Neo plasmid

pCMV-Atg2a-3×Myc-Neo plasmid

PVT98477

pCMV-Atg2a-3×Myc-Neo plasmid Information

Plasmid name: pCMV-Atg2a(mouse)-3×Myc-Neo
Alias: NM_194348.4;1810013C15Rik; A830054M12; mKIAA0404
Gene lenght: 5742bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Atg2a-3×Myc-Neo plasmid Description

pCMV-Atg2a-3×Myc-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-Atg2a-3×Myc-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGTTTAAAC TTAAGCTTGG 661 TACCGAGCTC GGATCCACTA GTCCAGTGTG GTGGAATTCG CCACCatgtc acgatggctg 721 tggccatggt cgaactgtgt gaaagagcgt gtctgccgct acttgctaca gcactacttg 781 ggtcacttct ttcaggagca cctcagcctg gaccagctca gcctggatct gtacaagggc 841 agcgttgccc tgcgggatat tcacctggaa acctggtctg tgaacgagtt tttgaggtca 901 atggagtcac cactggaact ggtggaaggc tttgtgagct ccatcgaggt ggctgtcccc 961 tgggctgcac ttctcactga ccactgcact gtgtgtgtgt caggccttca gcttaccctg 1021 cagccccgcc agggttcagg accaggggct gcggactctc agagttgggc ctcatgcatg 1081 accacaagct tgcagctggc tcaagagtgc cttcgggaag ggttgccaga accctcagaa 1141 ccaccacagc ccctggaggg gctggaaatg tttgcacaga ccattgagac tgtgctccgg 1201 aggatcaagg tgaccttcct gaacactgtt gtgagggtgg agcattcact gggtgatgag 1261 gatcgaagtg tggctgtgga ggtccgagtg cagagactgg agtactgtga cgaagcagtg 1321 cgggacccca gccaggcccc acctgtggac gtgcaccagc cgcctgcctt cctgcacaag 1381 ctgctgcagc tgagtggggt ctgcctatac tttgaggagc tgccctccca ggcagaccca 1441 ccacagccac ctctgcaaat cggcagctgc acggggtacg tggagctcat ggtgaggctg 1501 aagcagaacg aggcctttcc aggccccaag ttggaggtat caggacaact gggatccctg 1561 cacctgcttt tgaccccgcg tcagctccag cagctccaga ggctgctcag tgccgtcaac 1621 cttgcagacc ctgcaggcct ggccgacaag ctgaacaaga gccgtccact gggtgctgag 1681 gacttgtggc tcattgagca agatctgaac cagcagctgc aggcgggtgc tgtggctgag 1741 tccctcagcc tgtaccccat caccaacccc ctcaatttgg acagcacgga cctcttcttc 1801 tccatggccg gcctcacaag cagtgtgaca tcggctgtct ctgagctctc cgtgtacagc 1861 gtagacctgg gctcctctgt tcacagcaac atggccttcc accgaccctc aaccccaccc 1921 cactcgggtg gcaagatggc cccaactccc cttctggaca ccacacgccc tgactcactg 1981 gtgaagatga ccttgggcgg tgtgagcttg accttgctgc agactgcttc cccgtcttca 2041 ggaccatctg acctccccac ccactttttt gctgagttcg atgctgccaa agatgggcca 2101 ttcggctccc gggacttctc ccatctcagg ccacgcttcc agagggcctg cccttgtagc 2161 cacgtccggt taactggcac agccgtgcag ctgtcctggg agctgcgcac gggcagccac 2221 agccggagga caagcagcac cgaagtgcac tttggacagc tggaggtgct ggagtgccta 2281 tggcccaggg ctgccacaga gccagagtac acggagatcc tgagcttccc cagtcactct 2341 ggctccgaag cctcagcccg accctgcgcc catctgcgcc acacacagac cattcgccgg 2401 gtgctcaaga gccggtcccg gcgctccact gcctgccatt gtcactcaga actgtccctg 2461 gacctggccg acttccaatc tgatgtggag ctggggtccc tggaccgcct tgctgccttg 2521 ttccgtcaag tcaccacacc ctctgagcca cctgctggcc tactaacaga gcccccacag 2581 gccactgagc tgcagacagt gtttcgtctc tcagcacccc gggccaccct gaggctacgc 2641 ttccccatcc cagacctacg gcctgaccgg gacccctggg ctggccaggc tgtgcgggct 2701 gagcagctgc gactggagct gagtgagccc cagttccgct cagagctcaa cagtgggcct 2761 ggccccccag ctcctacccg cctggaactt acttgctcag acctgcaagg aatctacgaa 2821 gatggagaga agccaccagt cccctgcctg cgggtctcca aagctctgaa tcccaggagc 2881 actgaggcca agtactttct acctcaggta gtggtgaccc tgaaccccca gtccagtggc 2941 acacagtggg agacagccta tgagaaaggc cgggatctag agctgtccac cgagagtccg 3001 tgtgagctac agcaacctga gccctcgccc ttctcctcca agcggaccat gtacgagact 3061 gaggaaatgg tgatccctgg agaccctgaa gagatgagga cattccagag ccggacactg 3121 gcactgtccc gctgtactct ggatgtgatc atgcccagtg cccacatctt cctgcccagt 3181 aaggaggtct acgaaagtat ctacaacagg atcaataacg acctgctcat gtgggagcct 3241 gcagacctgc ttcccacatc cagcgctgct gctcggccgc ctggctcttc ggggttcaag 3301 atgtgcaagt cagccttcaa gctagactcg gattcagatg aagaagacgc ccagttcttc 3361 tcgatggcat caggtgtccc ccagacacct gccccagaac cttcccgtcg tcagtcccag 3421 agtaccttct ccacactggt gacagtgctg aagggtcgga tcactgcgct ctgtgaggct 3481 aaggatgaga cagggaagcg gctggatgtc actcacgggg agctggtact agatgtggag 3541 caaggcacca tcttcagcgt agcccagtac cgtggccaac cagggcttgg ctacttctgc 3601 ctggaagctg aaaaggccaa actctaccat cgagcggcca ttgaagacta cctgctaccc 3661 actcatttgg aagtgcccag ctttgctccc cccgctcagc tggctccaac catctaccca 3721 tcagaggaag gggtgactga gcgaggaacc ttgggccgca agggccaggg tccccccatg 3781 ctgtctgcag ctgtccgtat ccacctggac cctcacaaga atgtcaagga atttttggtg 3841 acagtacggc tgcacaaagc cactctacgc cactacatgg ccccacctga acagagctgg 3901 cactcccagc tcctggactt cttggatgtg ctggatgacc ctgtgctggg atacctgccc 3961 ccaaccgtca tcactgtcct gcacacacat ctcttctcct gcgctgtgga ctacaggccc 4021 ctctacctcc ctgtgcgtgt cctggtcact gctgagacct tcaccctctc cagcaacatt 4081 gtcatggaca cgtccacctt cctgctcagg ttcatccttg atgactccgc tctgtacctg 4141 tctgacaagt gtgaggtgga gagcctggat ctgcggcgag attatgtctg tgtcctggat 4201 attgacctcc tggaacttgt catcaaaacc tggaagggca gcactgaggg cagactgagc 4261 cagcctctgt ttgagctgcg ctgctccaac aacgtggtgc acgtgcatag ctgtgctgac 4321 tcctgcgccc tgctggtcaa cctgctccag tacctaacca gctcgggtga cctgcacccc 4381 ccaccccggc cccccagccc tacagagatc gctggccaga agctttcgga gagccctgcc 4441 tccctgccct catgcctacc tgtggagaca gccctcatca accaacgaga cctgactgac 4501 gcccttctgg atactgaacg gcgcggcctg caggagctgg cccagtcatc gggtggccca 4561 cttcctcagg cctctcccgt ctccgtctat ctgttcccag gtgaacggag tggggcccag 4621 gcccctttgc ctcctcctgg ggcctcttct cataccttgg gttctaaggc aaaggaacat 4681 gaaaacgaag aggaagggga cggagacacc ctggacagtg atgagttctg catccttgac 4741 gcccctggcc taggcattgc gcctcgagac ggggagccca tcgtgacaca gctgcatcca 4801 ggccccatca tcgtgcacga cggtcacttc tcccagcccc tgggaagcac tgacctgctg 4861 cgcgctcctg cccacttccc agtgcccagc agccgtgtgg tactacggga ggtctccttc 4921 atctggcacc tctatggggg tagagacttt ggccttcacc ccacttacag agcaagagta 4981 ggcctcacag gccccagggt ctcaccttcc cgctcctctg gtcccaaccg tccccagaac 5041 tcctggcgta cccagggtgg cattggacgg caacaccagg tcctcatgga gatccagctc 5101 agcaaagtga gcttccagca tgaagtgtac ccagaagagt cggccatagc tgggggcctg 5161 ggccaggagc tggacgagcg accactgtcg cgccaggtgc tcattgttca agagctagag 5221 atccgagacc ggctggccac ctccaagatc aacaagtttc ttcatttgca cacgagtgag 5281 cggctgccac ggcgcacaca ctccaacatg cttaccatca aggcactgca tgtggctccc 5341 accagcagcg tcggagggcc ggagtgctgt cttcgagtct ccatgatgcc tctgaggctc 5401 aatgtggacc aggatgccct cttcttcctc aaggactttt tcaccagcct agcagccagt 5461 atcaacccca tggtcccagg ggacacttct gaggctcccc gagagaccca cagccggccc 5521 ggcagccccc aggaagggca gtctgaggac acagagacag ccagcaaccc accggaagct 5581 ccaggcagta gccacagctc ctctgaccag cagcctatct acttcaggga gttccgcttc 5641 acatctgagg tgcccatctg cctggattac catggcaagc atgtcactgt ggaccaggtg 5701 ggcactttca tgggactcct aatcggcctg gcccagctca actgctcaga gctgaagctc 5761 aagaggctct gctgccggca tgggctcctg ggtgtggaca aggtgctgtg ctatgccctc 5821 aatgaatggc tgcaggacat ccgtaagaac cagctgcccg gcctgctggg aggcgtgggc 5881 cccatgcact cggttgtgca gctcttccaa gggttccggg acctgctgtg gctacccatc 5941 gagcagtaca ggaaagatgg gcgcctcatt cgggggctgc agcgtggggc tgcctccttt 6001 gggtcatcca cagcctctgc tgctctggaa ctgagcaaca ggctggtcca ggccatccag 6061 gctacagctg agacagtgta tgacatcctg tccccggctt ctcctgtctc ccgatcccta 6121 caagacaagc gctcttcgcg gaagctacgc agaggtcaac aacctgctga cctccgggaa 6181 ggcatggcta aggcttatga tgctgttcga gagggtatcc tggatacagc tcaaaccatc 6241 tgtgatgtgg catccagggg ccatgagcag aagggactga caggtgccgt ggggggtgtg 6301 atccgacagc tccccccaac cgtggtaaaa ccaataatcg tagcaacaga ggcaacatcc 6361 aacgtgctag ggggcatgcg taaccagatc cttccagatg cgcacaagga ccatgctctc 6421 aagtggcgat tggaggaggc ccaggacCTC GAGGAACAAA AACTAATATC AGAGGAAGAT 6481 TTGGAACAGA AACTAATCAG TGAAGAAGAT TTAGAGCAAA AGTTAATTTC CGAGGAGGAC 6541 TTATAGTGAG GGCCCGATAT CTCGACAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT 6601 TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC 6661 CTTTGTATCA TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT 6721 GGTTGCTGTC TCTTTATGAG GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA 6781 CTGTGTTTGC TGACGCAACC CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT 6841 CCGGGACTTT CGCTTTCCCC CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG 6901 CCCGCTGCTG GACAGGGGCT CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGTCGGGGA 6961 AGCTGACGTC CTTTCCATGG CTGCTCGCCT GTGTTGCCAC CTGGATTCTG CGCGGGACGT 7021 CCTTCTGCTA CGTCCCTTCG GCCCTCAATC CAGCGGACCT TCCTTCCCGC GGCCTGCTGC 7081 CGGCTCTGCG GCCTCTTCCG CGTCTTCGCC TTCGCCCTCA GACGAGTCGG ATCTCCCTTT 7141 GGGCCGCCTC CCCGCCTGGA AACGGGGGAG GCTAACTGAA ACACGGAAGG AGACAATACC 7201 GGAAGGAACC CGCGCTATGA CGGCAATAAA AAGACAGAAT AAAACGCACG GGTGTTGGGT 7261 CGTTTGTTCA TAAACGCGGG GTTCGGTCCC AGGGCTGGCA CTCTGTCGAT ACCCCACCGA 7321 GACCCCATTG GGGCCAATAC GCCCGCGTTT CTTCCTTTTC CCCACCCCAC CCCCCAAGTT 7381 CGGGTGAAGG CCCAGGGCTC GCAGCCAACG TCGGGGCGGC AGGCCCTGCC ATAGCAGATC 7441 TGCGCAGCTG GGGCTCTAGG GGGTATCCCC ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG 7501 CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC 7561 CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA 7621 ATCGGGGCAT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC 7681 TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT 7741 TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA 7801 ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GGGGATTTCG GCCTATTGGT 7861 TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTA ATTCTGTGGA ATGTGTGTCA 7921 GTTAGGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT 7981 CAATTAGTCA GCAACCAGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA 8041 AAGCATGCAT CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC 8101 CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA 8161 TGCAGAGGCC GAGGCCGCCT CTGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT 8221 TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA 8281 TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC 8341 TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG 8401 CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC 8461 CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC 8521 CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG 8581 GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA 8641 GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG 8701 CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG 8761 TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT 8821 CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC 8881 CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG 8941 GCTGGGTGTG GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA 9001 GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC 9061 GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC 9121 GCGAAATGAC CGACCAAGCG ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG 9181 CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC 9241 AGCGCGGGGA TCTCATGCTG GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA 9301 ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC 9361 ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA 9421 CCTCTAGCTA GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC 9481 CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT 9541 AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA 9601 ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA 9661 TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC 9721 GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG 9781 CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT 9841 TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA 9901 GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT 9961 CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC 10021 CTTCGGGAAG CGTGGCGCTT TCTCAATGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG 10081 TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT 10141 TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG 10201 CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA 10261 AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA 10321 AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG 10381 GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG 10441 AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG 10501 GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT 10561 GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT 10621 TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC 10681 TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA 10741 TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG 10801 GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT 10861 GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA 10921 TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT 10981 CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT 11041 TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG 11101 CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG 11161 AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG 11221 CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA 11281 AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT 11341 AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT 11401 GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT 11461 GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA 11521 TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT 11581 TTCCCCGAAA AGTGCCACCT GACGTCGACG GATCGGGAGA TCTCCCGATC CCCTATGGTC 11641 GACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATCTGC TCCCTGCTTG 11701 TGTGTTGGAG GTCGCTGAGT AGTGCGCGAG CAAAATTTAA GCTACAACAA GGCAAGGCTT 11761 GACCGACAAT TGCATGAAGA ATCTGCTTAG G

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.