pLV3-CMV-Elmo1-3×FLAG-ZsGreen1

pLV3-CMV-Elmo1-3×FLAG-ZsGreen1-Puro plasmid

PVT97209

pLV3-CMV-Elmo1-3×FLAG-ZsGreen1-Puro plasmid Information

Plasmid name: pLV3-CMV-Elmo1(mouse)-3×FLAG-ZsGreen1-Puro
Alias: NM_080288.2;6330578D22Rik; C230095H21Rik; CED-12
Gene lenght: 2181bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Elmo1-3×FLAG-ZsGreen1-Puro plasmid Description

pLV3-CMV-Elmo1-3×FLAG-ZsGreen1-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Elmo1-3×FLAG-ZsGreen1-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca 1441 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg 1501 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa 1561 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa 1621 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt 1681 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag 1741 gtggagagag agacagagac agatccattc gattagtgaa cggatctcga cggtatcgcc 1801 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata 1861 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aAATTTTCGG 1921 GTTTATTACA GGGACAGCAG AGATCCAGTT TATCGATAAG CTTGGGAGTT CCGCGTTACA 1981 TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG ACCCCCGCCC ATTGACGTCA 2041 ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG 2101 GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTACG 2161 CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA GTACATGACC 2221 TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG TCATCGCTAT TACCATGGTG 2281 ATGCGGTTTT GGCAGTACAT CAATGGGCGT GGATAGCGGT TTGACTCACG GGGATTTCCA 2341 AGTCTCCACC CCATTGACGT CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT 2401 CCAAAATGTC GTAACAACTC CGCCCCATTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG 2461 GAGGTCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA TCGCCTGGAG ACGCCATCCA 2521 CGCTGTTTTG ACCTCCATAG AAGACACCGA CTCTACTAGA GGATCTATTT CCGGTGAATT 2581 CGCCACCatg ccgccgcctt cggacatcgt gaaggtggcc atagaatggc ctggcgccta 2641 ccctaaactc atggagattg accagaaaaa gccactgtct gccattataa aggaagtctg 2701 tgatgggtgg tctcttgcca accatgaata ttttgcactg cagcacgctg atagttcaaa 2761 cttctacatc acagaaaaga atcgcaacga aataaaaaat ggcacgatcc ttcgattaac 2821 cacatctcca gctcagaatg cccagcagct ccatgaacga atacagtcat caagtatgga 2881 tgccaagttg gaagccctga aggacttggc cagcctctcc cgggatgtta cctttgctca 2941 ggagttcata aacctggatg gcatctcact tctcactcag atggtggaaa gtggcactga 3001 acgataccag aagttgcaga agattatgaa gccctgcttt ggagacatgc tgtccttcac 3061 tctcacagcc tttgtggagt tgatggacca cggcattgta tcatgggata cattctcagt 3121 ggccttcatt aagaagatag ccagctttgt gaacaagtct gccatagaca tctcaatcct 3181 ccagcggtcc ttggccattc tggagtcaat ggttctcaac agtcatgacc tctaccaaaa 3241 ggtagcccag gagatcacca ttggccaact cattcctcat cttcagggga cagaccaaga 3301 aatacaaact tacaccattg ctgtaatcaa cgcacttttc ctcaaagctc ctgatgagag 3361 gagacaggag atggcgaata ttttggctca gaagcagctg cgctacatca ttttaacaca 3421 tgtcatccga gcacagcggg ccatcaacaa tgagatggca caccagttat atgttctcca 3481 agtgctgacc tttaaccttc tggaagacag gatgatgact aagatggacc cccaggacca 3541 ggcccaaagg gacatcatat ttgaacttcg aagaattgct ttcgatgcag agtcagaacc 3601 taataacagc agtggcagca tggagaaacg caagtccatg tacactcggg attataaaaa 3661 acttggcttc attaatcacg tcaatcctgc catggacttc acacagaccc ctcctgggat 3721 gctggctctg gacaacatgc tgtatttcgc taagcaccac caggatgcat acatccggat 3781 cgtgctagag aacagcagcc gagaagataa acatgagtgc cccttcggcc gcagcagtat 3841 agagctgacc aagatgctgt gtgagatcct caaagtgggc gagctgccta gtgagacctg 3901 caacgacttt cacccgatgt tcttcaccca tgacagatct tttgaggaat tcttctgcat 3961 ttgcattcaa ctcctgaaca aaacatggaa ggaaatgagg gcaacatctg aagacttcaa 4021 caaggtaatg caggtggtga aggagcaggt tatgagagcg cttacaacca agcctagttc 4081 cctggaccag ttcaaaagca agctgcaaaa cctgagctac accgagatcc tgaaaatccg 4141 ccagtctgag aggatgaacc aggaagattt ccagtctcgc ccgattttgg aactaaagga 4201 gaagatccag ccagaaatct tagagctgat taaacagcag cgcctgaacc gccttgtgga 4261 agggacctgc tttaggaaac tcaatgctcg ccggagacaa gacaagtttt ggtattgtcg 4321 gctttcacca aatcacaagg tcttacatta tggcgacttg gaagagagcc cccaaggaga 4381 agtgccccac gattccctgc aggacaaatt gccggtggca gatatcaaag cagtggtgac 4441 gggaaaggac tgccctcata tgaaagagaa aggtgccctt aaacaaaaca aggaggtgct 4501 tgaacttgct ttctccatct tatacgactc aaattgccaa ctgaacttca ttgctcctga 4561 taagcatgag tactgcatct ggacagatgg gctgaatgca ctgcttggga aggacatgat 4621 gagtgacctg acacgcaatg acctggacac cctgctgagc atggagatca agcttcgcct 4681 gctggacctg gaaaacatcc agatcccgga tgcacctccg cctatcccca aggaacctag 4741 caactatgac tttgtctatg actgtaacGG ATCCTCGGTA CCAAGCTTAA GTGACTACAA 4801 GGATGACGAT GACAAGGATT ACAAAGACGA CGATGATAAG GACTATAAGG ATGATGACGA 4861 CAAATAAAGA TCCATCGATA CTAGTAAGGA TCTGCGATCG CTCCGGTGCC CGTCAGTGGG 4921 CAGAGCGCAC ATCGCCCACA GTCCCCGAGA AGTTGGGGGG AGGGGTCGGC AATTGAACGG 4981 GTGCCTAGAG AAGGTGGCGC GGGGTAAACT GGGAAAGTGA TGTCGTGTAC TGGCTCCGCC 5041 TTTTTCCCGA GGGTGGGGGA GAACCGTATA TAAGTGCAGT AGTCGCCGTG AACGTTCTTT 5101 TTCGCAACGG GTTTGCCGCC AGAACACAGC TGAAGCTTCG AGGGGCTCGC ATCTCTCCTT 5161 CACGCGCCCG CCGCCCTACC TGAGGCCGCC ATCCACGCCG GTTGAGTCGC GTTCTGCCGC 5221 CTCCCGCCTG TGGTGCCTCC TGAACTGCGT CCGCCGTCTA GGTAAGTTTA AAGCTCAGGT 5281 CGAGACCGGG CCTTTGTCCG GCGCTCCCTT GGAGCCTACC TAGACTCAGC CGGCTCTCCA 5341 CGCTTTGCCT GACCCTGCTT GCTCAACTCT ACGTCTTTGT TTCGTTTTCT GTTCTGCGCC 5401 GTTACAGATC CAAGCTGTGA CCGGCGCCTA CTCTAGCATG GCCCAGTCCA AGCACGGCCT 5461 GACCAAGGAG ATGACCATGA AGTACCGCAT GGAGGGCTGC GTGGACGGCC ACAAGTTCGT 5521 GATCACCGGC GAGGGCATCG GCTACCCCTT CAAGGGCAAG CAGGCCATCA ACCTGTGCGT 5581 GGTGGAGGGC GGCCCCTTGC CCTTCGCCGA GGACATCTTG TCCGCCGCCT TCATGTACGG 5641 CAACCGCGTG TTCACCGAGT ACCCCCAGGA CATCGTCGAC TACTTCAAGA ACTCCTGCCC 5701 CGCCGGCTAC ACCTGGGACC GCTCCTTCCT GTTCGAGGAC GGCGCCGTGT GCATCTGCAA 5761 CGCCGACATC ACCGTGAGCG TGGAGGAGAA CTGCATGTAC CACGAGTCCA AGTTCTACGG 5821 CGTGAACTTC CCCGCCGACG GCCCCGTGAT GAAGAAGATG ACCGACAACT GGGAGCCCTC 5881 CTGCGAGAAG ATCATCCCCG TGCCCAAGCA GGGCATCTTG AAGGGCGACG TGAGCATGTA 5941 CCTGCTGCTG AAGGACGGTG GCCGCTTGCG CTGCCAGTTC GACACCGTGT ACAAGGCCAA 6001 GTCCGTGCCC CGCAAGATGC CCGACTGGCA CTTCATCCAG CACAAGCTGA CCCGCGAGGA 6061 CCGCAGCGAC GCCAAGAACC AGAAGTGGCA CCTGACCGAG CACGCCATCG CCTCCGGCTC 6121 CGCCTTGCCC gcggccgctg agggcagagg aagtcttcta acatgcggtg acgtggagga 6181 gaatcccggc cctatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt 6241 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac 6301 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg 6361 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg 6421 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc 6481 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca 6541 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg 6601 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt 6661 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt 6721 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa 6781 gcccggtgcc tgaaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct 6841 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc 6901 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct 6961 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga 7021 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc 7081 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac 7141 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt 7201 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt 7261 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc 7321 tcttccgcgt ctccgccttc gccctcagac gagtcggatc tccctttggc cgcctccccg 7381 cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa 7441 agaaaagggg ggactggaag ggctaattca ctcccaacga aaataagatc tgctttttgc 7501 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg 7561 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg 7621 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat 7681 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg 7741 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa 7801 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 7861 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc 7921 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag 7981 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac 8041 ttttgcagag acggcccaaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt 8101 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt 8161 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg 8221 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg 8281 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 8341 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 8401 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 8461 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 8521 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 8581 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 8641 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 8701 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 8761 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 8821 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 8881 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg 8941 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 9001 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 9061 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 9121 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 9181 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 9241 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 9301 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 9361 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 9421 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 9481 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 9541 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 9601 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 9661 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 9721 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 9781 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 9841 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 9901 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 9961 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 10021 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 10081 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 10141 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 10201 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc 10261 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa 10321 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg 10381 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac 10441 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac 10501 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt 10561 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt 10621 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg 10681 acggccagtg ccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.