pCMV-ARHGAP35-3×FLAG-CMV-EGFP

pCMV-ARHGAP35-3×FLAG-CMV-EGFP plasmid

PVT110981

pCMV-ARHGAP35-3×FLAG-CMV-EGFP plasmid Information

Plasmid name: pCMV-ARHGAP35(human)-3×FLAG-CMV-EGFP
Alias: NM_004491.5;GRF-1; GRLF1; P190-A; P190A; p190ARhoGAP;p190RhoGAP
Gene lenght: 4497
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker:
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-ARHGAP35-3×FLAG-CMV-EGFP plasmid Description

pCMV-ARHGAP35-3×FLAG-CMV-EGFP It is a plasmid suitable for gene expression in mammalian cells.

pCMV-ARHGAP35-3×FLAG-CMV-EGFP plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc GCTAGCGCTA 601 CCGGACTCAG ATCTCGAGCT CCGAATTCGC CACCatgatg atggcaagaa agcaagatgt 661 ccgaattccc acctacaaca tcagtgtggt gggattatct gggaccgaga aggaaaaggg 721 ccagtgtggg attggaaagt cttgtttgtg caaccgcttc gtgcgcccga gtgctgacga 781 gtttcacttg gaccatacct ccgtcctcag caccagtgac tttggagggc gagtggtcaa 841 taatgaccac tttctctact ggggagaagt tagccgctcc ctggaggatt gtgtggaatg 901 taagatgcac attgtggagc agactgaatt tattgatgat cagacttttc aacctcatcg 961 aagcacggcc ctgcagccct atatcaagag agctgctgcg accaagcttg catcagctga 1021 aaaactcatg tacttttgca ctgaccagct ggggctggag caggactttg agcagaaaca 1081 aatgccagac ggaaagctgc tggttgatgg ttttcttctt ggtattgatg ttagcagggg 1141 catgaatagg aactttgatg accagctcaa gtttgtctcc aatctctaca atcagcttgc 1201 aaaaacaaaa aagcccatag tggtggtcct gactaagtgt gacgaaggtg ttgagcggta 1261 cattagagat gcacatactt ttgccttaag caaaaagaac ctccaggttg tggagacctc 1321 agcgagatcc aatgtaaacg tggacttggc tttcagcacc ttagtgcaac tcattgataa 1381 aagtcgggga aagacaaaaa tcattcctta ttttgaagct ctcaagcagc agagtcagca 1441 gatagctaca gcaaaagaca agtatgagtg gctggtgagt cgcattgtga aaaaccacaa 1501 tgagaactgg ctgagtgtca gccgaaagat gcaggcctct ccagaatacc aggactatgt 1561 ctacctggaa gggactcaga aagccaagaa gctgtttcta cagcacatcc accgcctcaa 1621 gcatgagcat atcgagcgta ggagaaagct gtacctggca gccctgccat tagcttttga 1681 agctcttata cctaatctag atgaaataga ccacctaagc tgcataaaag ccaaaaagct 1741 cttagaaacc aagccagaat tcttgaagtg gtttgttgtg cttgaagaga ccccatggga 1801 tgccaccagt cacattgaca acatggaaaa cgaacggatt ccctttgatt taatggatac 1861 cgtccctgca gagcagctat acgaggccca cttagagaag ctgaggaacg aaaggaaaag 1921 agttgagatg cgaagggcgt ttaaagaaaa cctggagact tctcctttca taactcccgg 1981 aaagccttgg gaagaggccc gtagttttat tatgaatgag gatttctacc agtggctgga 2041 ggaatctgta tacatggata tttatggcaa acaccaaaag caaattatag ataaagcaaa 2101 ggaagaattt caggagttgc ttttggaata ttcagaattg ttttatgaac tggagctgga 2161 tgctaagccc agcaaggaga agatgggtgt tattcaggat gttctgggag aggaacagcg 2221 atttaaagca ttacaaaagc tccaagcaga gcgtgatgcc cttattctga aacacattca 2281 ttttgtgtac cacccaacaa aggagacatg ccccagctgc ccagcttgtg tggacgctaa 2341 gattgagcac ttgattagtt ctcggtttat ccggccgtct gaccggaatc agaaaaattc 2401 actctctgac cctaacattg atagaatcaa cttggttata ttgggcaaag acggccttgc 2461 ccgagagttg gccaatgaga ttcgagctct ttgtacaaat gatgacaagt atgtgataga 2521 tggtaaaatg tatgagcttt ccctgaggcc aatagagggg aatgtcaggc ttcctgtgaa 2581 ctctttccag acgccaacat ttcagcccca cggctgtctc tgcctttaca attcaaagga 2641 atcgctatcc tatgtagtgg aaagtataga gaagagtaga gagtccacgc tgggccggcg 2701 ggataatcat ttagtccatc tcccccttac attaattttg gttaacaaga gaggagacac 2761 cagtggagag actctgcata gcttaataca gcaaggtcaa caaattgcta gcaaacttca 2821 gtgtgtcttt ctcgaccctg cttctgctgg cattggttac ggacgcaaca ttaatgaaaa 2881 gcaaatcagt caagttttga agggactcct ggactctaag cgtaacttaa acctggtcag 2941 ttctactgct agcatcaaag atttggctga tgttgatctg cgaattgtta tgtgtctgat 3001 gtgtggagat ccttttagtg cagatgacat actttttcct gtccttcagt cccaaacctg 3061 taaatcttcc cattgtggaa gcaacaactc tgttttactt gaactaccaa tcggactgca 3121 caagaagcgg attgaactgt ctgttctttc ataccattcc tcctttagca tcagaaagag 3181 ccggttggtt catgggtaca ttgtttttta ttcagccaaa cgtaaggcct ctttggctat 3241 gttacgtgcc tttctttgtg aagtgcagga tattatccct attcagcttg tagcactcac 3301 tgatggcgct gtagatgtcc tggacaatga cttaagtagg gaacagctaa ctgaggggga 3361 ggagattgct caagaaattg acggaaggtt cacaagcatc ccctgtagcc aaccccagca 3421 taaacttgag atctttcacc cattttttaa agatgtggtg gaaaaaaaga acataatcga 3481 ggctactcat atgtacgata atgctgccga ggcctgtagc accaccgaag aggtgtttaa 3541 ctccccccgg gcaggatcac cgctctgcaa ctcaaacctg caggattcag aagaagatat 3601 cgagccatct tacagcctgt ttcgagaaga cacatcactg ccttctctgt ccaaagacca 3661 ttctaagctc tctatggaac tggagggaaa tgatgggctg tctttcatta tgagcaattt 3721 tgagagtaaa ctgaacaaca aagtacctcc gccagtcaaa ccaaagcctc ctgtccattt 3781 tgaaattaca aagggggatc tatcttattt agaccaaggc catagggatg gacagaggaa 3841 gtctgtgtct tctagcccct ggctgcctca ggatgggttt gatccttctg actatgctga 3901 acccatggat gctgtggtga agccaaggaa tgaagaagaa aacatatact ccgtgcccca 3961 tgacagcacc caaggcaaaa tcatcaccat tcggaatatc aacaaagccc agtccaacgg 4021 cagcgggaat ggttctgaca gtgaaatgga caccagctct ctagagcgag ggcgcaaggt 4081 ttccatcgtg agcaagccag tgctgtacag gacgagatgc acccggctgg ggcggtttgc 4141 tagttaccgg accagcttca gcgtggggag tgatgatgag ctggggccca tccggaagaa 4201 agaggaggat caggcatccc agggttataa aggggacaat gctgtcattc catacgaaac 4261 agacgaagac ccgcggagga ggaatattct tcgcagccta aggaggaaca ctaagaaacc 4321 aaagcccaaa ccccggccat ccatcacaaa ggcaacctgg gagagtaact attttggggt 4381 gcccttaaca actgtcgtga ctccagagaa gccgatcccc atttttattg aaagatgtat 4441 tgagtacatt gaagccacag gactgagcac ggaaggcatc taccgggtca gcgggaacaa 4501 gtctgagatg gagagtctgc agagacagtt tgatcaagac cacaacctgg acctggcaga 4561 gaaagacttt acggtgaata ccgtggctgg tgccatgaag agctttttct cagaactgcc 4621 tgaccccctg gtcccgtata acatgcagat cgacttggtg gaagcacaca aaatcaacga 4681 ccgggagcag aagttgcatg cccttaagga ggtattaaag aaatttccaa aggaaaacca 4741 cgaagtcttc aagtatgtca tctctcacct aaacaaggtc agccacaaca acaaggtgaa 4801 tctcatgacc agcgagaacc tctccatctg cttctggccc accttgatga gacctgattt 4861 cagcactatg gacgccctca cagccacgcg cacctaccag acaatcattg aactctttat 4921 ccagcagtgc cccttcttct tctacaatcg gcccatcacc gagccccccg gcgccaggcc 4981 cagctccccc tctgccgtgg cttccaccgt ccccttcctc acttccacgc ctgtcacaag 5041 tcagccgtcg cccccacagt cgcctccacc caccccccag tccccaatgc agccactgct 5101 tccctcccag cttcaagccg aacacacgct gAAGCTTACG CGTGACTACA AAGACCATGA 5161 CGGTGATTAT AAAGATCATG ACATCGACTA CAAGGATGAC GATGACAAGT GAGGATCCGG 5221 GCCCGTTTAA ACCCGCTGAT CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT 5281 TTGCCCCTCC CCCGTGCCTT CCTTGACCCT GGAAGGTGCC ACTCCCACTG TCCTTTCCTA 5341 ATAAAATGAG GAAATTGCAT CGCATTGTCT GAGTAGGTGT CATTCTATTC TGGGGGGTGG 5401 GGTGGGGCAG GACAGCAAGG GGGAGGATTG GGAAGACAAT AGCAGGCATG CTGGGGATGC 5461 GGTGGGCTCT ATGGTCTAGA GATATACTGA GTCATTAGGG ACTTTCCAAT GGGTTTTGCC 5521 CAGTACATAA GGTCAATAGG GGTGAATCAA CAGGAAAGTC CCATTGGAGC CAAGTACACT 5581 GAGTCAATAG GGACTTTCCA TTGGGTTTTG CCCAGTACAA AAGGTCAATA GGGGGTGAGT 5641 CAATGGGTTT TTCCCATTAT TGGCACGTAC ATAAGGTCAA TAGGGGTGAG TCATTGGGTT 5701 TTTCCAGCCA aTTTAATTAA AACGCCATGT ACTTTCCCAC CATTGACGTC AATGGGCTAT 5761 TGAAACTAAT GCAACGTGAC CTTTAAACGG TACTTTCCCA TAGCTGATTA ATGGGAAAGT 5821 ACCGTTCTCG AGCCAATACA CGTCAATGGG AAGTGAAAGG GCAGCCAAAA CGTAACACCG 5881 CCCCGGTTTT CCCCTGGAAA TTCCATATTG GCACGCATTC TATTGGCTGA GCTGCGTTCT 5941 ACGTGGGTAT AAGAGGCGCG ACCAGCGTCG GTACCGTCGC AGTCTTCGGT CTGACCACCG 6001 TAGAACGCAG ATCaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg 6061 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg 6121 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg 6181 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct 6241 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag 6301 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg 6361 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca 6421 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct 6481 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca 6541 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg 6601 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc 6661 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc 6721 tcggcatgga cgagctgtac aagtaaagcg gccgcgactc tagatcataa tcagccatac 6781 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa 6841 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa 6901 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 6961 tggtttgtcc aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatattttgt 7021 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg 7081 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt 7141 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct 7201 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt 7261 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa 7321 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc 7381 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc 7441 tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat 7501 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc 7561 aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca 7621 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct 7681 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca 7741 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc 7801 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta 7861 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt 7921 tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga 7981 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct 8041 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc 8101 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag 8161 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg 8221 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc 8281 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc 8341 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg 8401 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc 8461 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg 8521 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc 8581 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag 8641 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg 8701 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg 8761 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc 8821 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt 8881 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca 8941 ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat 9001 gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg 9061 ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata 9121 cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct 9181 cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta 9241 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa 9301 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga 9361 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac 9421 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt 9481 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc 9541 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat 9601 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag 9661 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc 9721 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag 9781 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac 9841 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg 9901 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct 9961 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc 10021 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca 10081 t

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.