pLV2-TRE3GS-KAT7-TetOne-Hyg pl

pLV2-TRE3GS-KAT7-TetOne-Hyg plasmid

PVT97195

pLV2-TRE3GS-KAT7-TetOne-Hyg plasmid Information

Plasmid name: pLV2-TRE3GS-KAT7(human)-TetOne-Hyg
Alias: NM_007067.5;HBO1; HBOA; MYST2; ZC2HC7
Gene lenght: 1836bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression,Tetracycline induction
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Hyg
Fluorescent labeling:
Promoter: TRE3GS
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: TRE3GS-F1
Reverse primer:
Copy number: High
Induction:

pLV2-TRE3GS-KAT7-TetOne-Hyg plasmid Description

pLV2-TRE3GS-KAT7-TetOne-Hyg is a plasmid suitable for protein expression in mammalian cells, tetracycline-inducible, and can also be used for lentivirus packaging.

pLV2-TRE3GS-KAT7-TetOne-Hyg plasmid Sequence

1 actactaagt ttgtagtaca tatttaacaa atacaatttc tttaaaaact actaagtttg 61 tagtacatat ttaacaaata caatttcttt aaaatgaaaa taattcagag gaatcacagg 121 tttagagtaa atgaaaccac taggtaattg gcagtggtaa tagggtatgg ggtgggaagt 181 ttgggatgat tttggttagc ttgagttatc cagttgatcc agacatgata agatacattg 241 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt 301 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt gatctcccga 361 tccgtcgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 421 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 481 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 541 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 601 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 661 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 721 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat 781 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 841 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 901 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 961 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 1021 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 1081 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 1141 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 1201 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 1261 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 1321 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 1381 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 1441 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 1501 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 1561 ctgctgcttg caaacaaaaa aaccgctacc agcggtggtt tgtttgccgg atcaagagct 1621 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct 1681 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 1741 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 1801 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 1861 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 1921 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 1981 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 2041 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 2101 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 2161 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat 2221 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc 2281 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc 2341 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 2401 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc 2461 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga 2521 ccatgattac gccaagctct agctagaggt cgacggtaaa acgctagcaa tgtcaaggcc 2581 tctcactctc tgatattcat ttctttgcaa gttataaata ctgaataata agatgacatg 2641 aactactact gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac 2701 cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag 2761 gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc 2821 agagagaccc agtacaggca aaaagcagct gcttatatgc aggatctgag ggctcgccac 2881 tccccagtcc cgcccaggcc acgcctccct ggaaagtccc cagcggaaag tcccttgtag 2941 caagctcgat atcagcagtt cttgaagtac tccggatgca gctctcgggc cacgtgatga 3001 aatgctaggc ggctgtcaaa cctccactct aacacttctc tctccgggtc atccatccca 3061 tgcaggctca cagggtgtaa caagctggtg ttctctcctt tattggcctc ttctacctta 3121 tctggctcaa ctggtactag cttgtagcac catccaaagg tcagtggata tctgacccct 3181 ggccctggtg tgtagttctg ctaatcaggg aagtagcctt gtgtgtggta gatccacaga 3241 tcaaggatat cttgtcttcg ttgggagtga attagccctt ccagtcccct cttttctttt 3301 aaaaagtggc taagatctac agctgccttg taagtcattg gtcttaaagg tacctgaggt 3361 gtgactggaa aacccacctc ctcctcctct tgtgcttcta gccaggcaca atcagcattg 3421 gtagctgctg tattgctact tgtgattgct ccatgttttt ctaggtctcg actgcagaat 3481 taattccagg cggggaggcg gcccaaaggg agatccgact cgtctgaggg cgaaggcgaa 3541 gacgcggaag aggccgcaga gccggcagca ggccgcggga aggaaggtcc gctggattga 3601 gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat ccaggtggca acacaggcga 3661 gcagccatgg aaaggacgtc agcttccccg acaacaccac ggaattgtca gtgcccaaca 3721 gccgagcccc tgtccagcag cgggcaaggc aggcggcgat gagttccgcc gtggcaatag 3781 ggagggggaa agcgaaagtc ccggaaagga gctgacaggt ggtggcaatg ccccaaccag 3841 tgggggttgc gtcagcaaac acagtgcaca ccacgccacg ttgcctgaca acgggccaca 3901 actcctcata aagagacagc aaccaggatt tatacaagga ggagaaaatg aaagccatac 3961 gggaagcaat agcatgatac aaaggcatta aagcagcgta tccacatagc gtaaaaggag 4021 caacatagtt aagaatacca gtcaatcttt cacaaatttt gtaatccaga ggttgattgt 4081 tccagacgcg tctattcctt tgccctcgga cgagtgctgg ggcgtcggtt tccactatcg 4141 gcgagtactt ctacacagcc atcggtccag acggccgcgc ttctgcgggc gatttgtgta 4201 cgcccgacag tcccggctcc ggatcggacg attgcgtcgc atcgaccctg cgcccaagct 4261 gcatcatcga aattgccgtc aaccaagctc tgatagagtt ggtcaagacc aatgcggagc 4321 atatacgccc ggagccgcgg cgatcctgca agctccggat gcctccgctc gaagtagcgc 4381 gtctgctgct ccatacaagc caaccacggc ctccagaaga agatgttggc gacctcgtat 4441 tgggaatccc cgaacatcgc ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc 4501 gtcaggacat tgttggagcc gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc 4561 tcggcccaaa gcatcagctc atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc 4621 atcacagttt gccagtgata cacatgggga tcagcaatcg cgcatatgaa atcacgccat 4681 gtagtgtatt gaccgattcc ttgcggtccg aatgggccga acccgctcgt ctggctaaga 4741 tcggccgcag cgatcgcatc catggcctcc gcgaccggct gcagaacagc gggcagttcg 4801 gtttcaggca ggtcttgcaa cgtgacaccc tgtgcacggc gggagatgca ataggtcagg 4861 ctctcgctga attccccaat gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag 4921 tgccgataaa cataacgatc tttgtagaaa ccatcggcgc agctatttac ccgcaggaca 4981 tatccacgcc ctcctacatc gaagctgaaa gcacgagatt cttcgccctc cgagagctgc 5041 atcaggtcgg agacgctgtc gaacttttcg atcagaaact tctcgacaga cgtcgcggtg 5101 agttcaggct ttttcatcac gtgctgatca gatccgaaaa tggatataca agctcccggg 5161 agctttttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg gaatagctca 5221 gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga 5281 gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg cgggactatg 5341 gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac 5401 tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg 5461 gagcctgggg actttccaca ccctaactga cacacattcg cgcgtttacc cggggagcat 5521 gtcaaggtca aaatcgtcaa gagcgtcagc aggcagcata tcaaggtcaa agtcgtcaag 5581 ggcatcggct gggagcatgt ctaagtcaaa atcgtcaagg gcgtcggtcg gcccgccgct 5641 ttcgcacttt agctgtttct ccaggccaca tatgattagt tccaggccga aaaggaaggc 5701 aggttcggct ccctgccggt cgaacagctc aattgcttgt ttcagaagtg ggggcataga 5761 atcggtggta ggtgtctctc tttcctcttt tgctacttga tgctcctgtt cctccaatac 5821 gcagcccagt gtaaagtggc ccacggcgga cagagcgtac agtgcgttct ccagggagaa 5881 gccttgctga cacaggaacg cgagctgatt ttccagggtt tcgtactgtt tctctgttgg 5941 gcgggtgccg agatgcactt tagccccgtc gcgatgtgag aggagagcac agcggtatga 6001 cttggcgttg ttccgcagaa agtcttgcca tgactcgcct tccagggggc aggagtgggt 6061 atgatgcctg tccagcatct cgattggcag ggcatcgagc agggcccgct tgttcttcac 6121 gtgccagtac agggtaggct gctcaactcc cagcttttga gcgagtttcc ttgtcgtcag 6181 gccttcgata ccgactccat tgagtaattc cagagcagag tttatgactt tgctcttgtc 6241 cagtctagac atggtaattc gatgatcccc ctggggagag aggtcggtga ttcggtcaac 6301 gagggagccg actgccgacg tgcgctccgg aggcttgcag aatgcggaac accgcgcggg 6361 caggaacagg gcccacacta ccgccccaca ccccgcctcc cgcaccgccc cttcccggcc 6421 gctgctctcg gcgcgccctg ctgagcagcc gctattggcc acagcccatc gcggtcggcg 6481 cgctgccatt gctccctggc gctgtccgtc tgcgagggta ctagtgagac gtgcggcttc 6541 cgtttgtcac gtccggcacg ccgcgaaccg caaggaacct tcccgactta ggggcggagc 6601 aggaagcgtc gccggggggc ccacaagggt agcggcgaag atccgggtga cgctgcgaac 6661 ggacgtgaag aatgtgcgag acccagggtc ggcgccgctg cgtttcccgg aaccacgccc 6721 agagcagccg cgtccctgcg caaacccagg gctgccttgg aaaaggcgca accccaaccc 6781 cgtggaatta tcacctcgag tttactccct atcagtgata gagaacgtat gaagagttta 6841 ctccctatca gtgatagaga acgtatgcag actttactcc ctatcagtga tagagaacgt 6901 ataaggagtt tactccctat cagtgataga gaacgtatga ccagtttact ccctatcagt 6961 gatagagaac gtatctacag tttactccct atcagtgata gagaacgtat atccagttta 7021 ctccctatca gtgatagaga acgtataagc tttgcttatg taaaccaggg cgcctataaa 7081 agagtgctga ttttttgagt aaacttcaat tccacaacac ttttgtctta taccaacttt 7141 ccgtaccact tcctaccctc gtaaagaatt cccgtataca ccggtGCCAC Catgccgcga 7201 aggaagagga atgcaggcag tagttcagat ggaaccgaag attccgattt ttctacagat 7261 ctcgagcaca cagacagttc agaaagtgat ggcacatccc gacgatctgc tcgagtcacc 7321 cgctcctcag ccaggctaag ccagagttct caagattcca gtcctgttcg aaatctgcag 7381 tcttttggca ctgaggagcc tgcttactct accagaagag tgacccgtag tcagcagcag 7441 cctaccccag tgacaccgaa aaaataccct cttcggcaga ctcgttcatc tggttcagaa 7501 actgagcaag tggttgattt ttcagataga gaaactaaaa atacagctga tcatgatgag 7561 tcaccgcctc gaactccaac tggaaatgcg ccttcttctg agtctgacat agacatctcc 7621 agccccaatg tatctcacga tgagagcatt gccaaggaca tgtccctgaa ggactcaggc 7681 agtgatctct ctcatcgccc caagcgccgt cgcttccatg aaagctacaa cttcaatatg 7741 aagtgtccta caccaggctg taactctcta ggacacctta caggaaaaca tgagagacat 7801 ttctccatct caggatgccc actgtatcat aacctctcag ctgacgaatg caaggtgaga 7861 gcacagagcc gggataagca gatagaagaa aggatgctgt ctcacaggca agatgacaac 7921 aacaggcatg caaccaggca ccaggcacca acggagagac agcttcgata taaggaaaaa 7981 gtggctgaac tcaggaagaa aagaaattct ggactgagca aagaacagaa agagaaatat 8041 atggaacaca gacagaccta tgggaacaca cgggaacctc ttttagaaaa cctgacaagc 8101 gagtatgact tggatctttt ccgaagagca caagcccggg cttcagagga tttggagaag 8161 ttaaggctgc aaggccaaat cacagaggga agcaacatga ttaaaacaat tgcttttggc 8221 cgctatgagc ttgatacctg gtatcattct ccatatcctg aagaatatgc acggctggga 8281 cgtctctata tgtgtgaatt ctgtttaaaa tatatgaaga gccaaacgat actccgccgg 8341 cacatggcca aatgtgtgtg gaaacaccca cctggtgatg agatatatcg caaaggttca 8401 atctctgtgt ttgaagtgga tggcaagaaa aacaagatct actgccaaaa cctgtgcctg 8461 ttggccaaac tttttctgga ccacaagaca ttatattatg atgtggagcc cttcctgttc 8521 tatgttatga cagaggcgga caacactggc tgtcacctga ttggatattt ttctaaggaa 8581 aagaattcat tcctcaacta caacgtctcc tgtatcctta ctatgcctca gtacatgaga 8641 cagggctatg gcaagatgct tattgatttc agttatttgc tttccaaagt cgaagaaaaa 8701 gttggctccc cagaacgtcc actctcagat ctggggctta taagctatcg cagttactgg 8761 aaagaagtac ttctccgcta cctgcataat tttcaaggca aagagatttc tatcaaagaa 8821 atcagtcagg agacggctgt gaatcctgtg gacattgtca gcactctgca agcccttcag 8881 atgctcaaat actggaaggg aaaacaccta gttttaaaga gacaggacct gattgatgag 8941 tggatagcca aagaggccaa aaggtccaac tccaataaaa ccatggatcc cagctgctta 9001 aaatggaccc ctcccaaggg cacttaagga tccagaccac ctcccctgcg agctaagctg 9061 gacagccaat gacgggtaag agagtgacat ttttcactaa cctaagacag gagggccgtc 9121 agagctactg cctaatccaa agacgggtaa aagtgataaa aatgtatcac tccaacctaa 9181 gacaggcgca gcttccgagg gatttgagat ccagacatga taagatacat tgatgagttt 9241 ggacaaacca aaactagaat gcagtgaaaa aaatgcctta tttgtgaaat ttgtgatgct 9301 attgccttat ttgtaaccat tataagctgc aataaacaag ttaagtcgat aaactggatc 9361 tctgctgtcc ctgtaataaa cccgaaaatt ttgaattttt gtaatttgtt tttgtagttc 9421 tttagtttgt atgtctgttg ctattatgtc tactattctt tcccctgcac tgtacccccc 9481 aatcccccct tttcttttaa aggcgatacc gtcgagatcc gttcactaat cgaatggatc 9541 tgtctctgtc tctctctcca ccttcttctt ctattccttc gggcctgtcg ggtcccctcg 9601 gggttgggag gtgggtctga aacgataatg gtgaatatcc ctgcctaact ctattcacta 9661 tagaaagtac agcaaaaact attcttaaac ctaccaagcc tcctactatc attatgaata 9721 attttatata ccacagccaa tttgttatgt taaaccaatt ccacaaactt gcccatttat 9781 ctaattccaa taattcttgt tcattctttt cttgctggtt ttgcgattct tcaattaagg 9841 agtgtattaa gcttgtgtaa ttgttaattt ctctgtccca ctccatccag gtcgtgtgat 9901 tccaaatctg ttccagagat ttattactcc aactagcatt ccaaggcaca gcagtggtgc 9961 aaatgagttt tccagagcaa ccccaaatcc ccaggagctg ttgatccttt aggtatcttt 10021 ccacagccag gattcttgcc tggagctgct tgatgcccca gactgtgagt tgcaacagat 10081 gctgttgcgc ctcaatagcc ctcagcaaat tgttctgctg ctgcactata ccagacaata 10141 attgtctggc ctgtaccgtc agcgtcattg acgctgcgcc catagtgctt cctgctgctc 10201 ccaagaaccc aaggaacaaa gctcctattc ccactgctct tttttctctc tgcaccactc 10261 ttctctttgc cttggtgggt gctactccta atggttcaat ttttactact ttatatttat 10321 ataattcact tctccaattg tccctcatat ctcctcctcc aggtctgaag atcagcggcc 10381 ggccgcttgc tgtgcggtgg tcttactttt gttttgctct tcctctatct tgtctaaagc 10441 ttccttggtg tcttttatct ctatcctttg atgcacacaa tagagggttg ctactgtatt 10501 atataatgat ctaagttctt ctgatcctgt ctgaagggat ggttgtagct gtcccagtat 10561 ttgtctacag ccttctgatg tttctaacag gccaggatta actgcgaatc gttctagctc 10621 cctgcttgcc catactatat gttttaattt atattttttc tttccccctg gccttaaccg 10681 aattttttcc catcgcgatc taattctccc ccgcttaata ctgacgctct cgcacccatc 10741 tctctccttc tagcctccgc tagtcaaaat ttttggcgta ctcaccagtc gccgcccctc 10801 gcctcttgcc gtgcgcgctt cagcaagccg agtcctgcgt cgagagagct cctctggttt 10861 ccctttcgct ttcaagtccc tgttcgggcg ccactgctag agattttcca cactgactaa 10921 aagggtctga gggatctcta gttaccagag tcacacaaca gacgggcaca cactacttga 10981 agcactcaag gcaagcttta ttgaggctta agcagtgggt tccctagtta gccagagagc 11041 tcccaggctc agatctggtc taaccagaga gacccagtac aggcaaaaag cagctgctta 11101 tatgcaggat ctgagggctc gccactcccc agtcccgccc aggccacgcc tccctggaaa 11161 gtccccagcg gaaagtccct tgtagcaagc tcgatatcag cagttcttga agtactccgg 11221 atgcagctct cgggccacgt gatgaaatgc taggcggctg tcaaacctcc actctaacac 11281 ttctctctcc gggtcatcca tcccatgcag gctcacaggg tgtaacaagc tggtgttctc 11341 tcctttattg gcctcttcta ccttatctgg ctcaactggt actagcttgt agcaccatcc 11401 aaaggtcagt ggatatctga cccctggccc tggtgtgtag ttctgctaat cagggaagta 11461 gccttgtgtg tggtagatcc acagatcaag gatatcttgt cttctttggg agtgaattag 11521 cccttcca

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.