pCMV-PFAS-3×FLAG-Neo plasmid

pCMV-PFAS-3×FLAG-Neo plasmid

PVT97174

pCMV-PFAS-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-PFAS(human)-3×FLAG-Neo
Alias: NM_012393.3;FGAMS; FGAR-AT; FGARAT; GATD8; PURL;
Gene lenght: 4017bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-PFAS-3×FLAG-Neo plasmid Description

pCMV-PFAS-3×FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-PFAS-3×FLAG-Neo plasmid Sequence

1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA 841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC 901 GTTTAAACTT AAGCTTGGTA CCGAGCTCGG ATCCACTAGT CCAGTGTGGT GGAATTCGCC 961 ACCatgtccc cagtccttca cttctatgtt cgtccctctg gccatgaggg ggcagcccct 1021 ggacacactc ggaggaaact gcaagggaaa ctgccagagc tgcagggcgt cgagactgaa 1081 ctgtgctaca acgtgaactg gacagctgag gccctcccca gtgctgagga gacaaagaag 1141 ctgatgtggc tgtttggttg ccccttactg ctggatgatg ttgctcggga gtcctggctc 1201 cttcctggct ccaatgacct gctgctggag gtcgggccca ggctgaactt ctccacccca 1261 acatccacca acatcgtgtc agtgtgccgc gccactgggc tggggcctgt ggatcgtgtg 1321 gagaccaccc ggcgctaccg gctctcgttt gcccaccccc cgtcagctga ggtggaagcc 1381 attgctctgg ctaccctgca cgaccggatg acagagcagc acttccccca tcccatccag 1441 agtttctccc ctgagagcat gccggaaccc ctcaatggcc ctatcaatat actgggtgag 1501 ggccggcttg cgctggagaa ggccaaccag gagcttggtc tggctttaga ctcttgggac 1561 ctagacttct acaccaagcg cttccaggag ctacagcgga acccgagcac tgtggaggcc 1621 tttgacttgg cgcagtccaa tagcgagcac agccgacact ggttcttcaa gggccagctc 1681 cacgtggatg ggcagaagct ggtgcactca ctgtttgagt ccatcatgag cacccaggaa 1741 tcctcgaacc ccaacaacgt cctcaaattc tgtgataaca gcagtgcaat ccagggaaag 1801 gaagtccgat tcctacggcc tgaggacccc acacggccaa gccgcttcca gcaacagcaa 1861 gggctgagac atgttgtctt cacagcagag actcacaact ttcccacagg agtatgcccc 1921 tttagtggtg caaccactgg cacagggggc cggattcgag atgtccagtg cacaggccgc 1981 ggggcccacg tggtggctgg cactgccggc tattgctttg gaaatctgca tattccaggt 2041 tacaatctgc cctgggagga tccaagcttc cagtatcctg ggaattttgc ccggcccctg 2101 gaggttgcca ttgaagccag taatggagct tctgactatg gcaacaagtt tggggaacca 2161 gtgctggctg gcttcgcccg ctccttgggc ctccagctcc cagacggcca gcggcgtgag 2221 tggatcaagc ccatcatgtt tagtgggggc attgggtcca tggaagctga ccacataagc 2281 aaggaggccc cagagccagg catggaagtt gtaaaggttg gaggtcccgt ctacaggatt 2341 ggagttggag gtggagctgc ttcatctgtg caggtgcagg gagataacac cagtgacctg 2401 gactttgggg ctgtgcagcg aggagacccg gagatggaac agaagatgaa ccgtgtgatc 2461 agggcttgtg tggaggcccc caagggaaac cccatctgca gccttcatga tcagggcgct 2521 ggtggcaatg gcaatgtcct aaaagagctg agtgacccag ctggagccat catttacacc 2581 agccgcttcc agcttgggga cccaaccctg aatgccctgg aaatctgggg ggctgagtac 2641 caggaatcaa atgctcttct gctgaggtcc cccaaccggg acttcctgac tcatgtcagt 2701 gcccgtgaac gttgcccggc ttgcttcgtg ggcaccatca ctggagaccg gagaatagtg 2761 ctggtggacg atcgggagtg tcctgtcaga agaaatggcc agggggatgc ccccccgaca 2821 cccctgccaa cccctgtgga cctggagctc gaatgggtgc tgggcaagat gcctcggaag 2881 gagttcttcc tgcagaggaa gccccccatg ctgcagcctc tggccttgcc cccagggctg 2941 agcgtgcacc aggctctgga gagggttctg aggctgcccg ccgtggccag caagcgctac 3001 ctcaccaata aggtggaccg ctctgtggga ggcctggtgg cccagcagca gtgcgtgggg 3061 cccctgcaaa ctcctctggc agatgtagcg gttgtggcac tgagccatga ggagctcata 3121 ggggctgcca cagccttggg agaacagcca gtcaagagcc tgctggaccc aaaagtcgcc 3181 gcccggctgg ccgtggccga agccctcacc aacctggtgt ttgctctggt cactgacctc 3241 cgggatgtga agtgtagcgg gaactggatg tgggcagcca agctcccagg ggagggcgca 3301 gctttggcgg atgcctgtga ggctatggtg gcagtgatgg cagccctggg tgtggcagtg 3361 gatggtggca aggactccct cagcatggct gctcgggttg gcactgagac cgtgcgggct 3421 cctgggtcac tggtcatctc agcctatgcc gtctgcccag acatcacagc cactgtgacc 3481 ccagacctca agcatcctga agggagaggc catctgctct atgtggctct gagccctggg 3541 cagcaccggc tcgggggcac agctctggcc cagtgcttct cccagcttgg ggaacaccct 3601 ccagacctgg accttcctga gaacttggtg cgggccttca gcatcactca ggggctgctg 3661 aaagaccgcc tcctctgctc aggccacgat gtcagtgacg gaggcctcgt cacatgcctg 3721 ctggagatgg cctttgctgg aaattgcggg ctacaggtgg atgtgcctgt ccccagggtt 3781 gatgtcctgt ctgtgctgtt cgctgaggag ccaggcctcg tgctggaggt gcaggagcca 3841 gacctggccc aggtgctgaa gcgttaccgg gatgctggcc tccattgcct ggagctgggc 3901 cacacaggcg aggccgggcc ccacgccatg gtccgggtgt cagtgaacgg ggctgtggtt 3961 ctggaggagc ctgttgggga gctgcgagcc ctctgggagg agacgagttt ccagctggac 4021 cggctacagg cagagcctcg ctgtgtggca gaggaggaac ggggcctgag ggagcggatg 4081 gggcccagct attgcctgcc ccccaccttt cccaaagcct ccgtgccccg tgagcctggt 4141 ggtcccagcc cccgagtcgc catcttgcga gaggagggca gtaatggaga ccgggagatg 4201 gccgatgcct tccacttagc tgggtttgag gtatgggacg tgaccatgca ggacctctgc 4261 tctggggcaa ttgggctgga cactttccgt ggcgtggcct tcgtgggcgg cttcagctat 4321 gcagatgtcc tgggctctgc caaagggtgg gcagctgctg tgacctttca tcccagggct 4381 ggggctgagc tgaggcgctt ccggaagcgg ccagacacct tcagcctggg cgtgtgtaat 4441 ggctgtcaac tgctggctct gctcggctgg gtgggaggcg accccaatga ggatgctgca 4501 gagatgggcc ctgactccca gccagcccgg ccaggccttc tgctacgcca caacctgtct 4561 gggcgctacg agtctcgctg ggccagcgtg cgtgtggggc ctgggccagc cctgatgctg 4621 cgagggatgg agggcgccgt gctgcccgtg tggagtgcgc acggggaagg ttacgtagca 4681 ttttcttctc cggaactcca agctcagatt gaggccaggg gcttggctcc actgcactgg 4741 gctgatgatg acgggaaccc cacagagcag taccctctga atcccaatgg gtccccaggg 4801 ggcgtggctg gcatctgctc ctgtgatggc cgccacctgg ctgtcatgcc tcaccctgag 4861 cgggccgtta ggccttggca gtgggcatgg cgaccccctc catttgatac tctgaccacc 4921 tccccctggc tccagctctt tatcaatgcc cgaaactgga ccctggaagg gagctgcCTC 4981 GAGGACTACA AAGACCATGA CGGTGATTAT AAAGATCATG ACATCGACTA CAAGGATGAC 5041 GATGACAAGT AGTGAGGGCC CGTTTAAACC CGCTGATCAG CCTCGACTGT GCCTTCTAGT 5101 TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC GTGCCTTCCT TGACCCTGGA AGGTGCCACT 5161 CCCACTGTCC TTTCCTAATA AAATGAGGAA ATTGCATCGC ATTGTCTGAG TAGGTGTCAT 5221 TCTATTCTGG GGGGTGGGGT GGGGCAGGAC AGCAAGGGGG AGGATTGGGA AGACAATAGC 5281 AGGCATGCTG GGGATGCGGT GGGCTCTATG GCTTCTGAGG CGGAAAGAAC CAGCTGGGGC 5341 TCTAGGGGGT ATCCCCACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 5401 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 5461 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 5521 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 5581 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 5641 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 5701 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 5761 ATTTAACAAA AATTTAACGC GAATTAATTC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA 5821 AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA 5881 CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA 5941 ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA 6001 GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG 6061 CCGCCTCTGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT 6121 TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT 6181 GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG 6241 TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG 6301 TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG 6361 CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC 6421 CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG 6481 AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA 6541 TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC 6601 AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG 6661 ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG 6721 CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA 6781 TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG 6841 ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT 6901 GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT 6961 TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGAAA TGACCGACCA 7021 AGCGACGCCC AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT 7081 GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT 7141 GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG 7201 CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT 7261 GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA GCTAGAGCTT 7321 GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA 7381 CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT 7441 CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT 7501 GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC 7561 TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA 7621 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 7681 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 7741 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 7801 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 7861 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 7921 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 7981 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 8041 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 8101 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 8161 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 8221 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTTTTTTTGT 8281 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 8341 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 8401 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 8461 AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT 8521 CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC 8581 TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG 8641 CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG 8701 TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT 8761 AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT 8821 GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT 8881 TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT 8941 CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT 9001 TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT 9061 CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC 9121 CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA 9181 ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA 9241 CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA 9301 AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT 9361 TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA 9421 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC 9481 TGACGTC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.