pCMV-USP24-Neo plasmid

pCMV-USP24-Neo plasmid

PVT97131

pCMV-USP24-Neo plasmid Information

Plasmid name: pCMV-USP24(human)-Neo
Alias: NM_015306.3
Gene lenght: 7863bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): BL21(DE3)
Growth Temperature: 30°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: Low
Induction:

pCMV-USP24-Neo plasmid Description

pCMV-USP24-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-USP24-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC 901 Cgccaccatg gaatcggagg aggagcagca catgaccacg ctgctgtgca tgggcttctc 961 agaccccgcc accatccgca aggccctgcg cctggccaag aacgacatta acgaggccgt 1021 ggcactgctc accaacgagc ggccgggcct cgactacggc ggctacgagc ccatggacag 1081 cggcggtggc cccagccccg ggcccggcgg gggcccgcgg ggcgacggcg gaggtgacgg 1141 cggcggcggc ggcccctccc gcggcgggag caccggaggc gggggcggct tcgacccccc 1201 gcccgcctac cacgaggtgg tggacgcgga gaagaatgat gagaatggaa actgctcagg 1261 ggaaggaatt gaattcccta caacaaattt atatgaactg gaaagccgtg ttttgactga 1321 tcattggtcc atcccttaca agcgagaaga atcactaggc aaatgcctgt tggcatctac 1381 ctacctagca agacttggtc tttccgagtc tgatgagaat tgtagaaggt ttatggacag 1441 gtgtatgcct gaagcattta aaaagctcct gacatcaagt gctgttcaca agtggggtac 1501 tgaaattcat gaaggaattt acaacatgtt gatgctatta atagaactgg tcgcagagag 1561 aataaaacaa gatccaattc ccactggtct cctgggtgtg cttacaatgg ctttcaatcc 1621 tgataatgaa taccatttta aaaacagaat gaaagtgtct caaaggaatt gggcagaagt 1681 gtttggagag ggaaatatgt ttgctgtttc acctgtatcg actttccaaa aggagcctca 1741 tggatgggtt gtggatttgg taaataagtt tggagaatta ggtggatttg cagcaatcca 1801 agccaagctc cattcagaag atatagaact tggggctgtc tcagcactga ttcagccctt 1861 aggagtgtgt gcagagtacc tcaattcctc cgtggtacag cccatgctag acccagtcat 1921 tcttactaca atccaggatg tacggagtgt agaagagaaa gacctcaaag acaagagatt 1981 ggttagcatc cctgagctct tgtctgccgt taagttactt tgcatgcgct tccaaccgga 2041 tctggtgaca attgtggatg accttcgact agatattcta ttgcgcatgc tgaaatcacc 2101 acatttcagt gctaagatga attctctcaa agaagtaacc aaactaatag aagatagcac 2161 tttatccaaa tctgtgaaga atgctataga tacagacaga ttattagatt ggctagttga 2221 aaactcagtt ctgtcgattg cactggaagg caacatagac caagcacaat actgtgaccg 2281 tataaaggga attattgaac tcttgggtag taaattgtcg ttagatgaac tcactaaaat 2341 ttggaagata cagtcaggac aatcatctac tgtgattgag aacattcata ctattattgc 2401 tgcagcggct gtgaaattta attcagatca gcttaatcat ttgtttgttc tcattcagaa 2461 gagctgggag actgagagtg atagagtaag acagaagctt ttgagcctga ttggacgaat 2521 aggccgggaa gctcgctttg agaccacttc tggaaaggtt ttagacgtac tctgggaact 2581 ggctcacctt ccaaccctgc ccagtagcct tattcagcag gccttggagg agcacctgac 2641 aatccttagt gatgcatatg cagtgaaaga agcaatcaag aggagctaca tcatcaagtg 2701 catagaagat attaagaggc ctggagaatg gtcaggtttg gaaaaaaaca agaaggatgg 2761 attcaagtca tctcagctta ataatcccca gtttgtatgg gtggtaccag ctttgcgtca 2821 gctccatgaa attactcgct cattcataaa acaaacctat caaaagcaag acaagagcat 2881 tattcaagac ttgaagaaga attttgaaat agtgaaattg gtaacgggaa gtttgatcgc 2941 ttgtcatcgg cttgcagctg ctgtggccgg gcctggaggc ttaagtggct cgacactagt 3001 ggatggccgg tacacttacc gggagtattt agaggcacat ctaaaatttc tagcgttttt 3061 cttgcaagaa gctactctgt atctgggctg gaatcgtgcc aaggagatct gggagtgtct 3121 tgtaactggc caggatgttt gtgaattaga tagagagatg tgttttgaat ggtttacaaa 3181 aggacagcat gatcttgaga gtgatgttca gcagcagctc ttcaaggaga aaattcttaa 3241 attggagtca tatgaaatca ctatgaatgg ttttaactta tttaaaactt tttttgaaaa 3301 tgtgaatctt tgtgatcatc gattgaaaag acaaggagct cagttgtatg tagaaaagct 3361 ggaattgata ggaatggatt tcatttggaa aatagccatg gaatcacctg atgaagaaat 3421 tgctaatgaa gctattcagc taatcataaa ctatagttac attaatctaa atcctagatt 3481 aaagaaggat tcagtatctt tacataagaa attcattgct gattgctaca caagattaga 3541 agcagccagt tcagcacttg gtggccccac tctaacacat gctgtgacca gagcaacaaa 3601 aatgcttaca gcaactgcca tgccaactgt agcaacctca gttcagtctc cttatagatc 3661 tactaaactt gtaataattg agagattgct gcttctggca gagcgctatg tgatcactat 3721 agaggatttt tactctgttc cacgaactat tctacctcat ggtgcctcat ttcatggaca 3781 tcttttaacc cttaatgtta cctatgagtc taccaaagat accttcactg tcgaggctca 3841 cagtaatgaa accataggga gtgtccggtg gaaaatagcc aagcagttgt gctctcctgt 3901 ggataatata cagatattta caaatgatag cctgctgaca gtgaataaag atcaaaagct 3961 actccaccaa ctgggctttt ctgatgaaca aatccttaca gtgaagactt ctggcagtgg 4021 gaccccatct gggagttcag cagattcttc aaccagctcc agcagcagca gcagtggggt 4081 ttttagttct tcatatgcca tggagcagga gaaatccctc cctggtgtag tgatggctct 4141 cgtatgtaac gtatttgaca tgctttatca gctcgccaat ctggaagagc caaggataac 4201 tctacgagta cggaagcttc tgctcttgat acccactgat ccagccattc aggaagccct 4261 tgatcaactt gattctttag gaagaaagaa aacattgctg tctgaatcaa gttctcagtc 4321 ctcaaaatct ccatccctgt catcaaagca acagcaccag ccaagtgcca gttcaatttt 4381 agaaagtctg tttcgatctt ttgccccggg aatgtctacc ttcagagtgc tctacaactt 4441 agaagttcta agctccaaac tcatgccaac agctgatgat gacatggcca gaagctgtgc 4501 caaatccttc tgtgaaaact tcctcaaagc tggcggtttg agtttggttg taaatgtcat 4561 gcagagagac tccatcccat cagaagtaga ctatgaaaca aggcagggtg tttattccat 4621 ctgtctacag cttgcaagat ttttacttgt cggacaaaca atgcccacgt tattagatga 4681 agacctcacc aaagatggta tagaagcact ttcttcccgc ccattccgaa atgtcagccg 4741 gcagacaagc agacagatgt ccttatgtgg taccccagaa aagtcatcct accgacagtt 4801 gtccgtgtct gataggtctt ctattagggt tgaggaaatc atccctgctg ctcgagttgc 4861 aatacaaaca atggaagtaa gtgatttcac ttctactgtg gcttgcttca tgagattgtc 4921 atgggctgcg gctgcaggac ggcttgatct tgttgggagt agccagccaa ttaaagaaag 4981 taattccctg tgtcctgctg gaattcgaaa cagactcagc agttcaggaa gcaattgcag 5041 ctctggaagt gaaggagaac cagtagccct gcatgcggga atctgtgttc gacaacagtc 5101 tgtatccacc aaagactcgc tgattgcggg agaggctttg tctcttcttg ttacgtgcct 5161 acagcttcgg agccagcaac tggcatcttt ctataacttg ccctgtgttg ctgatttcat 5221 cattgatatt ctgctcggat caccaagtgc tgagattcgc cgggttgcct gtgatcagct 5281 gtacactctt agtcagacag acacatcagc gcatccagat gtgcagaagc caaatcagtt 5341 tcttctaggc gtaatcctca cggctcagct gcctctctgg tctccaacta gtattatgag 5401 aggagtcaat cagagactgt tatctcagtg tatggagtat tttgatttga gatgccagtt 5461 attagatgat ctgacaactt cagaaatgga gcagttaagg atcagcccag ctacgatgct 5521 tgaagatgag attacttggc tggataactt tgaacctaat cgtacagctg aatgtgagac 5581 cagtgaagcg gacaacatct tactggcagg gcacttacgc ctcatcaaga cccttctttc 5641 actctgtggg gcagaaaagg aaatgcttgg ttcatcactc attaaaccat tgttagatga 5701 cttccttttc cgagcttcta gaattatttt aaatagtcat tctccagctg gcagtgccgc 5761 catcagtcaa caggactttc atccaaagtg tagtacagcg aatagccgat tggcagccta 5821 tgaagtcctt gtgatgttgg ctgatagttc accttcaaat cttcaaatta ttataaaaga 5881 actgctttct atgcatcacc agcctgaccc tgctcttacc aaggagtttg attaccttcc 5941 cccagtggat agcaggtcca gttcagggtt tgtggggctg agaaatggtg gtgcaacttg 6001 ttatatgaat gcagtcttcc agcagctgta tatgcaacct gggctccctg agtcattact 6061 ttcagtggat gatgacacag acaatccaga tgatagcgtg ttttaccaag tgcagtctct 6121 ctttggacat ttaatggaaa gcaagctgca gtactatgta cctgagaatt tttggaagat 6181 tttcaagatg tggaataaag aactttatgt gagagaacag caggatgcat atgaattctt 6241 tactagtctc attgatcaga tggatgaata cctcaagaaa atggggagag accaaatttt 6301 taagaataca tttcagggca tctactctga tcagaagatc tgtaaagact gtcctcacag 6361 atatgagcgt gaagaagctt tcatggctct caatctagga gtgacttctt gtcagagttt 6421 ggaaatttct ttggaccaat ttgttagagg agaagttcta gaaggaagta atgcgtacta 6481 ctgtgaaaag tgtaaagaaa agagaataac agtgaaaagg acctgtatta aatctttacc 6541 tagcgtcttg gtaattcacc taatgagatt tgggtttgac tgggaaagcg gacgctccat 6601 taaatatgat gaacaaataa ggtttccctg gatgctaaac atggagcctt acacagtttc 6661 aggaatggct cgccaagatt cttcttctga agttggggaa aatgggcgaa gtgtggatca 6721 gggcggtgga ggatccccac gaaaaaaggt tgccctcaca gaaaactatg aacttgtcgg 6781 tgtcatcgta cacagtgggc aggcacacgc aggccactac tattccttca ttaaggacag 6841 gcgagggtgt ggaaaaggaa agtggtataa atttaatgac acagttatag aagaatttga 6901 cctaaatgac gagaccctgg agtatgaatg ctttggagga gaatatagac caaaagttta 6961 tgatcaaaca aacccataca ctgatgtgcg ccgaagatac tggaatgcct atatgctttt 7021 ctaccaaagg gtgtctgatc agaactcccc agtattacca aagaaaagtc gagtcagcgt 7081 tgtacggcag gaagctgagg atctctctct gtcagctcca tcttcaccag aaatttcacc 7141 tcagtcatcc cctcggcccc ataggccgaa caatgaccgg ctgtctattc ttaccaagct 7201 ggttaaaaaa ggcgagaaga aaggactgtt tgtggagaaa atgcctgctc gaatatacca 7261 gatggtgaga gatgagaacc tcaagtttat gaagaataga gatgtataca gtagtgatta 7321 tttcagtttt gttttgtctt tagcttcatt gaatgctact aaattaaagc atccatatta 7381 tccttgcatg gcaaaggtga gcttacagct tgctattcaa ttcctttttc aaacttatct 7441 acggacaaag aagaaactca gggttgatac tgaagaatgg attgctacca ttgaagcatt 7501 gctttcaaaa agttttgatg cttgtcagtg gttagttgaa tattttatta gttctgaagg 7561 acgagaattg ataaagattt tcttactgga gtgcaatgtg agagaagtac gagttgctgt 7621 ggccaccatt ctggagaaaa ccctagacag tgccttgttt tatcaggata agttaaaaag 7681 ccttcatcag ttactggagg tactacttgc tctgttggac aaagacgtcc cagaaaattg 7741 taaaaactgt gctcagtact ttttcctgtt caacactttt gtacaaaagc aaggaattag 7801 ggctggagat cttcttctga ggcattcagc tctgcggcac atgatcagct tcctcctagg 7861 ggccagtcgg caaaacaatc agatacgtcg atggagttca gcacaagcac gagaatttgg 7921 gaatcttcac aatacagtgg cgttacttgt tttgcattca gatgtctcat cccaaaggaa 7981 tgttgctcct ggcatattta agcaacgacc acccattagc attgctccct caagccctct 8041 gttgcccctc catgaggagg tagaagcctt gttgttcatg tctgaaggga aaccttacct 8101 gttagaggta atgtttgctt tgcgggagct gacaggctcg ctcttggcac tcattgagat 8161 ggtagtgtac tgctgtttct gtaatgagca tttttccttc acaatgctgc atttcattaa 8221 gaaccaacta gaaacggctc cacctcatga gttaaagaat acgttccaac tacttcatga 8281 aatattggtt attgaagatc ctatacaagt agagcgagtc aaatttgtgt ttgagacaga 8341 aaatggatta ctagctttga tgcaccacag taatcatgtg gacagtagtc gctgctacca 8401 gtgtgtcaaa tttcttgtca ctcttgctca aaagtgtcct gcagctaagg agtacttcaa 8461 ggagaattcc caccactgga gctgggctgt gcagtggcta cagaagaaga tgtcagaaca 8521 ttactggaca ccacagagta atgtctctaa tgaaacatca actggaaaaa cctttcagcg 8581 aaccatttca gctcaggaca cgttagcgta tgccacagct ttgttgaatg aaaaagagca 8641 atcaggaagc agtaatgggt cggagagtag tcctgccaat gagaacggag acaggcatct 8701 acagcagggt tcagaatctc ccatgatgat tggtgagttg agaagtgacc ttgatgatgt 8761 tgatccctag CTCGAGTCTA GAGGGCCCGT TTAAACCCGC TGATCAGCCT CGACTGTGCC 8821 TTCTAGTTGC CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG 8881 TGCCACTCCC ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG 8941 GTGTCATTCT ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA 9001 CAATAGCAGG CATGCTGGGG ATGCGGTGGG CTCTATGGCT TCTGAGGCGG AAAGAACCAG 9061 CTGGGGCTCT AGGGGGTATC CCCACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT 9121 GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC 9181 TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG 9241 GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA 9301 GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT 9361 GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT 9421 CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA 9481 TGAGCTGATT TAACAAAAAT TTAACGCGAA TTAATTCTGT GGAATGTGTG TCAGTTAGGG 9541 TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG 9601 TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 9661 CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT 9721 CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG 9781 GCCGAGGCCG CCTCTGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC 9841 CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAAGAG 9901 ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC 9961 GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT 10021 GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG 10081 TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG 10141 GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA 10201 TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA 10261 TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC 10321 GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC 10381 GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG 10441 CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG 10501 CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT 10561 GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC 10621 GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC 10681 ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GACTCTGGGG TTCGAAATGA 10741 CCGACCAAGC GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCC GCCTTCTATG 10801 AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG 10861 ATCTCATGCT GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA 10921 AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT 10981 GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG ACCTCTAGCT 11041 AGAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA 11101 TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA 11161 GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT 11221 GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT 11281 CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT 11341 CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA 11401 ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT 11461 TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT 11521 GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC 11581 GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA 11641 GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT 11701 CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA 11761 ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG 11821 GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC 11881 CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA 11941 CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTT 12001 TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA 12061 TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA 12121 TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT 12181 CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG 12241 CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT 12301 AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG 12361 ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC 12421 GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG 12481 CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA 12541 TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA 12601 GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA 12661 TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA 12721 ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA 12781 AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG 12841 ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG 12901 GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG 12961 CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG 13021 GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC 13081 TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA 13141 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG 13201 TGCCACCTGA CGTCGACGGA TCGGGAGATC TCCCGATCCC CTATGGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.