pCMV-PIEZO2-FLAG-IRES-Puro pla

pCMV-PIEZO2-FLAG-IRES-Puro plasmid

PVT97129

pCMV-PIEZO2-FLAG-IRES-Puro plasmid Information

Plasmid name: pCMV-PIEZO2(human)-FLAG-IRES-Puro
Alias: NM_001378183.1;C18orf30; C18orf58; DA3; DA5; DAIPT;FAM38B; FAM38B2; HsT748; HsT771; MWKS
Gene lenght: 8598bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): BL21(DE3)
Growth Temperature: 30°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: Low
Induction:

pCMV-PIEZO2-FLAG-IRES-Puro plasmid Description

pCMV-PIEZO2-FLAG-IRES-Puro is a plasmid suitable for protein expression in mammalian cells.

pCMV-PIEZO2-FLAG-IRES-Puro plasmid Sequence

1 CGTCTGTTGT GTGACTCTGG TAACTAGAGA TCCCTCAGAC CCTTTTAGTC AGTGTGGAAA 61 ATCTCTAGCA GTAGTAGTTC ATGTCATCTT ATTATTCAGT ATTTATAACT TGCAAAGAAA 121 TGAATATCAG AGAGTGAGAG GAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC 181 AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG 241 TCCAAACTCA TCAATGTATC TTATCATGTC TGGCTCTAGC TATCCCGCCC CTAACTCCGC 301 CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT 361 TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CGGCCTCTGA GCTATTCCAG AAGTAGTGAG 421 GAGGCTTTTT TGGAGGCCTA GGGACGTACC CAATTCGCCC TATAGTGAGT CGTATTACGC 481 GCGCTCACTG GCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT 541 TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC 601 CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGGACGCGC CCTGTAGCGG 661 CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC 721 CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC 781 CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT 841 CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC 901 GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC 961 TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT 1021 TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTTAACAA 1081 AATATTAACG CTTACAATTT AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT 1141 TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA 1201 ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT 1261 ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA 1321 GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC 1381 AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT 1441 AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT 1501 CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT 1561 CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC 1621 ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG 1681 CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC 1741 ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA 1801 CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG 1861 GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT 1921 GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT 1981 GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA 2041 CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC 2101 CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC 2161 TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC 2221 CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG 2281 CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG 2341 GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA 2401 AATACTGTTC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG 2461 CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG 2521 TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA 2581 ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC 2641 CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT 2701 CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC 2761 TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA 2821 TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC 2881 CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG 2941 GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG 3001 CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC 3061 GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC 3121 AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC 3181 TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA 3241 AACAGCTATG ACCATGATTA CGCCAAGCGC GCAATTAACC CTCACTAAAG GGAACAAAAG 3301 CTGGAGCTGC AAGCTTAATG TAGTCTTATG CAATACTCTT GTAGTCTTGC AACATGGTAA 3361 CGATGAGTTA GCAACATGCC TTACAAGGAG AGAAAAAGCA CCGTGCATGC CGATTGGTGG 3421 AAGTAAGGTG GTACGATCGT GCCTTATTAG GAAGGCAACA GACGGGTCTG ACATGGATTG 3481 GACGAACCAC TGAATTGCCG CATTGCAGAG ATATTGTATT TAAGTGCCTA GCTCGATACA 3541 TAAACGGGTC TCTCTGGTTA GACCAGATCT GAGCCTGGGA GCTCTCTGGC TAACTAGGGA 3601 ACCCACTGCT TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT TCAAGTAGTG TGTGCCCGTC 3661 TGTTGTGTGA CTCTGGTAAC TAGAGATCCC TCAGACCCTT TTAGTCAGTG TGGAAAATCT 3721 CTAGCAGTGG CGCCCGAACA GGGACTTGAA AGCGAAAGGG AAACCAGAGG AGCTCTCTCG 3781 ACGCAGGACT CGGCTTGCTG AAGCGCGCAC GGCAAGAGGC GAGGGGCGGC GACTGGTGAG 3841 TACGCCAAAA ATTTTGACTA GCGGAGGCTA GAAGGAGAGA GATGGGTGCG AGAGCGTCAG 3901 TATTAAGCGG GGGAGAATTA GATCGCGATG GGAAAAAATT CGGTTAAGGC CAGGGGGAAA 3961 GAAAAAATAT AAATTAAAAC ATATAGTATG GGCAAGCAGG GAGCTAGAAC GATTCGCAGT 4021 TAATCCTGGC CTGTTAGAAA CATCAGAAGG CTGTAGACAA ATACTGGGAC AGCTACAACC 4081 ATCCCTTCAG ACAGGATCAG AAGAACTTAG ATCATTATAT AATACAGTAG CAACCCTCTA 4141 TTGTGTGCAT CAAAGGATAG AGATAAAAGA CACCAAGGAA GCTTTAGACA AGATAGAGGA 4201 AGAGCAAAAC AAAAGTAAGA CCACCGCACA GCAAGCGGCC GCTGATCTTC AGACCTGGAG 4261 GAGGAGATAT GAGGGACAAT TGGAGAAGTG AATTATATAA ATATAAAGTA GTAAAAATTG 4321 AACCATTAGG AGTAGCACCC ACCAAGGCAA AGAGAAGAGT GGTGCAGAGA GAAAAAAGAG 4381 CAGTGGGAAT AGGAGCTTTG TTCCTTGGGT TCTTGGGAGC AGCAGGAAGC ACTATGGGCG 4441 CAGCGTCAAT GACGCTGACG GTACAGGCCA GACAATTATT GTCTGGTATA GTGCAGCAGC 4501 AGAACAATTT GCTGAGGGCT ATTGAGGCGC AACAGCATCT GTTGCAACTC ACAGTCTGGG 4561 GCATCAAGCA GCTCCAGGCA AGAATCCTGG CTGTGGAAAG ATACCTAAAG GATCAACAGC 4621 TCCTGGGGAT TTGGGGTTGC TCTGGAAAAC TCATTTGCAC CACTGCTGTG CCTTGGAATG 4681 CTAGTTGGAG TAATAAATCT CTGGAACAGA TTTGGAATCA CACGACCTGG ATGGAGTGGG 4741 ACAGAGAAAT TAACAATTAC ACAAGCTTAA TACACTCCTT AATTGAAGAA TCGCAAAACC 4801 AGCAAGAAAA GAATGAACAA GAATTATTGG AATTAGATAA ATGGGCAAGT TTGTGGAATT 4861 GGTTTAACAT AACAAATTGG CTGTGGTATA TAAAATTATT CATAATGATA GTAGGAGGCT 4921 TGGTAGGTTT AAGAATAGTT TTTGCTGTAC TTTCTATAGT GAATAGAGTT AGGCAGGGAT 4981 ATTCACCATT ATCGTTTCAG ACCCACCTCC CAACCCCGAG GGGACCCGAC AGGCCCGAAG 5041 GAATAGAAGA AGAAGGTGGA GAGAGAGACA GAGACAGATC CATTCGATTA GTGAACGGAT 5101 CTCGACGGTA TCGGTTTTAA AAGAAAAGGG GGGATTGGGG GGTACAGTGC AGGGGAAAGA 5161 ATAGTAGACA TAATAGCAAC AGACATACAA ACTAAAGAAT TACAAAAACA AATTACAAAA 5221 ATTCAAAATT TTATCGATAA GCTTGGGAGT TCCGCGTTAC ATAACTTACG GTAAATGGCC 5281 CGCCTGGCTG ACCGCCCAAC GACCCCCGCC CATTGACGTC AATAATGACG TATGTTCCCA 5341 TAGTAACGCC AATAGGGACT TTCCATTGAC GTCAATGGGT GGAGTATTTA CGGTAAACTG 5401 CCCACTTGGC AGTACATCAA GTGTATCATA TGCCAAGTAC GCCCCCTATT GACGTCAATG 5461 ACGGTAAATG GCCCGCCTGG CATTATGCCC AGTACATGAC CTTATGGGAC TTTCCTACTT 5521 GGCAGTACAT CTACGTATTA GTCATCGCTA TTACCATGGT GATGCGGTTT TGGCAGTACA 5581 TCAATGGGCG TGGATAGCGG TTTGACTCAC GGGGATTTCC AAGTCTCCAC CCCATTGACG 5641 TCAATGGGAG TTTGTTTTGG CACCAAAATC AACGGGACTT TCCAAAATGT CGTAACAACT 5701 CCGCCCCATT GACGCAAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT ATAAGCAGAG 5761 CTCGTTTAGT GAACCGTCAG ATCGCCTGGA GACGCCATCC ACGCTGTTTT GACCTCCATA 5821 GAAGACACCG ACTCTAGCTA GAGGATCCCC CGGGCTGCAG ACTAGTGATA TCGCCACCAT 5881 GGCCTCAGAA GTGGTGTGCG GGCTCATCTT CAGGCTGCTG CTGCCCATCT GCCTGGCAGT 5941 AGCATGTGCA TTCCGATACA ATGGGCTCTC CTTTGTCTAC CTTATCTACC TCTTGCTCAT 6001 TCCTCTGTTC TCAGAACCAA CAAAAACGAC GATGCAAGGA CATACGGGAC GGTTATTAAA 6061 GTCTCTGTGC TTCATCAGTC TTTCCTTCCT GTTGCTGCAC ATCATTTTCC ACATCACGTT 6121 GGTGAGCCTT GAAGCTCAAC ATCGTATTGC ACCTGGCTAC AACTGCTCAA CATGGGAAAA 6181 GACATTCCGG CAGATCGGCT TTGAAAGCTT AAAGGGAGCT GATGCTGGCA ATGGGATCAG 6241 AGTGTTTGTA CCTGACATCG GGATGTTCAT TGCTAGTCTG ACCATCTGGC TCCTCTGTAG 6301 AAACATTGTT CAGAAACCTG TGACAGACGA AGCAGCACAG AGTAACCCGG AGTTTGAAAA 6361 TGAAGAATTG GCTGAAGGAG AAAAAATTGA TTCAGAAGAG GCACTGATCT ATGAAGAGGA 6421 TTTCAATGGA GGAGATGGTG TTGAAGGCGA GTTGGAAGAA AGCACGAAGT TAAAAATGTT 6481 CCGCAGGCTT GCCTCTGTGG CCTCTAAGCT CAAGGAGTTC ATTGGCAACA TGATCACCAC 6541 TGCTGGGAAA GTCGTTGTTA CCATCTTACT GGGCTCCTCG GGCATGATGT TGCCGTCTTT 6601 GACATCATCT GTGTATTTTT TTGTATTTTT GGGTCTGTGC ACCTGGTGGT CCTGGTGCCG 6661 GACGTTCGAC CCATTGCTGT TCAGCTGTCT CTGTGTTCTG CTGGCTATTT TCACTGCTGG 6721 ACATTTGATT GGACTTTATT TATACCAGTT CCAATTCTTT CAAGAGGCAG TTCCACCCAA 6781 TGACTACTAT GCAAGGTTGT TTGGTATCAA GTCAGTAATT CAAACGGACT GTTCAAGTAC 6841 TTGGAAGATC ATAGTGAACC CGGACCTGTC GTGGTACCAC CACGCCAACC CTATCCTCCT 6901 GCTGGTGATG TACTACACTC TGGCCACTCT GATCCGCATC TGGCTGCAAG AGCCCCTTGT 6961 GCAGGATGAG GGGACCAAAG AAGAGGACAA AGCCCTGGCT TGTAGCCCCA TCCAAATAAC 7021 AGCGGGGAGG AGGCGGAGCC TGTGGTACGC AACCCATTAC CCCACTGATG AGAGAAAACT 7081 TTTATCCATG ACCCAGGATG ACTACAAACC ATCTGATGGC CTGCTGGTGA CTGTGAACGG 7141 CAACCCCGTG GATTACCACA CCATCCACCC AAGCCTGCCC ATGGAGAACG GCCCTGGCAA 7201 AGCCGACCTC TACTCCACCC CTCAGTACCG GTGGGAGCCC TCTGATGAAT CCTCAGAAAA 7261 GCGAGAGGAG GAAGAGGAAG AGAAAGAAGA ATTTGAAGAA GAAAGGAGCC GTGAGGAAAA 7321 AAGAAGTATC AAAGTTCATG CCATGGTCTC CGTATTCCAA TTTATTATGA AACAAAGTTA 7381 CATCTGTGCC CTCATAGCTA TGATGGCCTG GAGCATCACC TATCACAGCT GGCTGACCTT 7441 CGTGCTGCTG ATCTGGTCGT GCACTCTTTG GATGATTCGC AACAGAAGAA AATATGCCAT 7501 GATCAGCTCT CCCTTCATGG TGGTTTATGG AAACCTATTG TTGATATTAC AGTATATATG 7561 GAGTTTTGAA CTTCCTGAAA TTAAAAAAGT TCCAGGATTT TTAGAAAAGA AAGAGCCAGG 7621 AGAACTTGCT TCCAAAATCC TTTTCACCAT TACTTTTTGG CTACTGCTGA GGCAGCACCT 7681 CACAGAGCAA AAAGCTCTGC AAGAAAAGGA AGCTCTTTTA TCGGAAGTCA AAATTGGCAG 7741 TCAGGAAAAT GAAGAAAAAG ATGAGGAACT TCAAGATATA CAAGTGGAAG GAGAGCCCAA 7801 AGAGGAGGAA GAAGAGGAAG CGAAGGAAGA GAAGCAAGAG AGAAAGAAGG TAGAGCAAGA 7861 GGAAGCTGAA GAAGAAGATG AGCAGGACAT CATGAAAGTC CTGGGCAATC TGGTGGTGGC 7921 CATGTTCATC AAGTACTGGA TCTACGTCTG CGGAGGCATG TTCTTCTTCG TCAGCTTCGA 7981 GGGTAAAATC GTAATGTACA AAATCATCTA CATGGTGCTG TTCCTGTTCT GTGTGGCCCT 8041 ATACCAGGTG CACTATGAAT GGTGGAGGAA AATTCTAAAA TATTTTTGGA TGTCAGTGGT 8101 TATTTACACT ATGCTGGTGC TTATCTTTAT ATACACATAT CAGTTTGAGA ACTTCCCAGG 8161 CCTGTGGCAA AATATGACTG GACTGAAAAA AGAAAAGCTT GAGGATCTTG GCTTAAAGCA 8221 GTTTACTGTG GCTGAACTAT TCACTCGCAT ATTCATCCCA ACCTCCTTTC TGCTGGTGTG 8281 CATTTTACAC CTGCACTACT TCCATGACCG GTTCCTTGAA CTCACAGACC TCAAGTCCAT 8341 TCCCAGCAAA GAAGACAACA CCATCTACAG CCATGCCAAA GTCAATGGTC GCGTATATCT 8401 AATAATAAAT AGCATCAAGA AAAAATTACC AATCCACCAA AATGAACTGG CCCACCCGGA 8461 AGGAAGCCTC CCGGACCTCA CCATGATGCA TCTGACTGCC AGCCTGGAGA AGCCGGAGGT 8521 GAGGAAGTTG GCTGAGCCTG GGGAGGAGAA GCTTGAGGGC TACTCTGAAA AAGCCCAGAA 8581 GGGTGATCTT GGGAAAGACA GCGAGGAGTC AGAGGAGGAC GGAGAGGAAG AGGAGGAATC 8641 CGAGGAGGAG GAAGAAACAT CAGACTTAAG GAACAAATGG CACCTGGTGA TTGACCGCCT 8701 CACTGTGCTC TTCTTAAAAT TCCTGGAGTA TTTTCACAAG CTGCAGGTGT TCATGTGGTG 8761 GATTTTGGAG TTGCACATCA TCAAAATCGT TTCCTCTTAC ATTATCTGGG TTTCTGTGAA 8821 AGAGGTGTCT CTGTTCAACT ATGTATTTTT GATTTCTTGG GCTTTTGCTC TGCCGTACGC 8881 CAAGCTGCGC CGTCTGGCTT CAAGTGTCTG CACAGTCTGG ACGTGTGTGA TCATCGTCTG 8941 CAAAATGTTG TACCAGCTCC AAACCATTAA GCCTGAGAAC TTCTCTGTTA ACTGTTCCTT 9001 GCCAAATGAA AATCAAACAA ACATCCCCTT TAATGAGTTG AACAAGTCTC TGCTCTACAG 9061 CGCTCCTATC GATCCTACAG AGTGGGTCGG CCTGCGGAAG TCTTCGCCTC TGCTAGTCTA 9121 CCTGAGGAAT AACCTCCTGA TGCTGGCTAT CCTGGCCTTT GAAGTCACCA TTTACCGCCA 9181 TCAGGAATAC TATCGAGGTC GAAATAACCT GACGGCCCCT GTGTCTAGAA CTATCTTTCA 9241 TGACATTACA AGACTACATC TAGATGATGG ACTTATTAAT TGTGCCAAAT ATTTCATTAA 9301 TTACTTCTTT TACAAGTTTG GTCTGGAGAC CTGTTTCCTA ATGTCAGTTA ACGTCATTGG 9361 CCAGCGAATG GATTTCTATG CCATGATCCA CGCCTGCTGG CTGATCGCTG TCTTATATAG 9421 ACGCAGAAGG AAAGCCATCG CAGAGATCTG GCCCAAGTAC TGCTGCTTCC TGGCATGCAT 9481 CATCACCTTC CAGTATTTCA TCTGCATTGG CATCCCACCT GCTCCTTGCC GAGATTACCC 9541 GTGGAGATTC AAGGGTGCCA GCTTCAATGA CAACATCATA AAGTGGCTGT ACTTCCCAGA 9601 TTTCATTGTG CGGCCCAACC CTGTGTTTCT CGTCTATGAC TTCATGCTGC TTCTGTGTGC 9661 CTCCTTACAA CGGCAGATTT TTGAGGATGA GAACAAGGCT GCAGTGCGAA TCATGGCAGG 9721 TGACAATGTC GAGATCTGCA TGAACCTTGA TGCGGCCTCC TTCAGCCAAC ATAACCCTGT 9781 GCCAGATTTT ATTCACTGCA GATCTTACTT AGACATGTCC AAAGTGATCA TCTTCAGCTA 9841 CCTCTTCTGG TTTGTGCTCA CCATCATCTT CATCACTGGG ACCACCAGGA TCAGCATCTT 9901 TTGCATGGGG TACCTGGTGG CCTGTTTCTA CTTCCTGCTC TTTGGGGGCG ATTTGCTGTT 9961 GAAACCCATC AAGAGCATCC TGCGCTACTG GGACTGGCTG ATCGCATACA ACGTTTTTGT 10021 GATTACGATG AAAAATATCC TGTCAATAGG AGCATGTGGA TACATTGGAA CATTGGTGCA 10081 CAATAGTTGT TGGTTGATCC AGGCTTTCAG CCTGGCCTGC ACAGTCAAAG GCTATCAAAT 10141 GCCTGCTGCT AATTCACCCT GTACACTTCC CAGTGGGGAA GCAGGAATCA TTTGGGACAG 10201 CATATGTTTT GCCTTCCTCC TGCTGCAAAG AAGAGTTTTC ATGAGTTATT ATTTTCTACA 10261 TGTTGTGGCT GATATAAAAG CTTCCCAGAT TCTGGCATCA AGAGGAGCTG AACTTTTCCA 10321 GGCCACAATT GTAAAAGCTG TAAAGGCAAG AATTGAGGAA GAGAAGAAGT CCATGGACCA 10381 GCTGAAGCGA CAGATGGATC GCATCAAGGC CAGGCAACAG AAATATAAAA AGGGTAAGGA 10441 GAGGATGCTG AGCTTGACCC AGGAGCCAGG GGAAGGCCAG GACATGCAAA AACTCTCTGA 10501 AGAGGATGAT GAAAGAGAAG CAGACAAACA GAAAGCCAAG GGCAAAAAAA AGCAGTGGTG 10561 GCGGCCTTGG GTTGATCATG CTTCCATGGT CAGGAGTGGA GATTATTATT TGTTTGAAAC 10621 GGATAGTGAA GAGGAGGAAG AGGAAGAATT AAAGAAGGAA GATGAAGAAC CTCCACGAAG 10681 GTCAGCATTC CAGAGAGCTA TTGGGAAGTT TGCATCAGCC ATTTTAGCCT TGCCAAAGTC 10741 TGTCATTAAA CTTCCCAAAA CTATTCTTCA GTATTTAATC AGAGCTGCAA AGTTTGTTTA 10801 TCAAGCCTGG ATTACTGATC CTAAAACAGC ACTCCGACAA AGACACAAAG AGAAAAAAAG 10861 GTCTGCAAGA GAAGAACGGA AACGAAGGCG GAAAGGATCC AAGGAGGGTC CTGTGGAATG 10921 GGAAGACCGG GAGGATGAAC CAATCAAAAA GAAATCCGAT GGACCAGATA ATATCATCAA 10981 GAGGATATTT AATATTTTGA AATTTACCTG GGTCCTATTT CTGGCAACAG TGGACAGTTT 11041 CACTACTTGG CTTAACTCCA TTTCAAGGGA GCATATTGAT ATATCTACAG TTCTGAGAAT 11101 TGAACGATGC ATGCTGACCA GAGAAATTAA GAAGGGCAAT GTTCCAACTC GGGAGAGCAT 11161 CCACATGTAC TATCAGAACC ACATCATGAA CCTTTCCAGA GAGTCGGGAC TGGACACCAT 11221 TGACGAGCAT CCCGGAGCTG CTTCAGGTGC ACAGACAGCC CACAGGATGG ATAGTTTAGA 11281 TTCACATGAC AGTATCTCCA GCTGCTACAC TGAAGCAACC ATGCTGTTCT CAAGGCAGTC 11341 CACCCTTGAT GATTTAGATG GACAAGAAAT TCCTAAAACA AGCGAGCGTG CTCGGCCTAG 11401 GCTCCGTAAA ATGCTCAGCA TGGATATGTC CTCCTCATCA GCTGACAGTG GCAGTTTAGC 11461 ATCAAGCGAG CCCACGCAGT GTACCATGCT GTACTCACGC CAGGGGACCA CTGAGACCAT 11521 CGAGGAGGTG GAGGCTGAGC AGGAGGAGGA GGCAGGGAGC ACGGCGCCTG AGCCCAGGGA 11581 GGCCAAGGAG TACGAGGCCA CTGGGTACGA TGTGGGAGCC ATGGGTGCCG AGGAGGCCAG 11641 CCTCACCCCA GAGGAAGAGC TGACACAGTT CTCCACCTTG GACGGGGATG TGGAGGCCCC 11701 ACCCTCCTAC AGCAAGGCTG TGAGCTTCGA GCATCTGTCC TTCGGCTCGC AGGACGACTC 11761 TGCAGGCAAG AACCGTATGG CAGTCAGCCC GGACGACAGC CGCACCGACA AGCTGGGGTC 11821 CAGCATCTTA CCTCCCCTGA CCCATGAGCT GACGGCCAGC GAGCTGCTGC TGAAAAAGAT 11881 GTTTCACGAC GATGAGCTTG AAGAGTCAGA GAAATTCTAC GTGGGGCAGC CCCGATTTCT 11941 GCTGCTCTTC TATGCCATGT ACAATACCCT GGTGGCCCGC TCGGAGATGG TGTGCTACTT 12001 CGTGATCATC CTCAACCACA TGGTCTCTGC CTCCATGATC ACGCTCCTGC TTCCCATCCT 12061 CATCTTCCTC TGGGCCATGT TGTCCGTCCC CAGGCCCAGC CGCCGGTTCT GGATGATGGC 12121 CATCGTCTAT ACTGAGGTGG CAATTGTAGT CAAGTATTTC TTCCAATTTG GGTTCTTTCC 12181 CTGGAATAAG AATGTGGAGG TGAACAAAGA TAAACCGTAT CACCCCCCAA ACATCATAGG 12241 AGTGGAAAAG AAGGAAGGTT ATGTTCTCTA TGACCTCATC CAGCTCCTGG CTCTGTTCTT 12301 TCATCGATCA ATTTTGAAGT GCCATGGCTT ATGGGATGAA GATGACATGA CTGAAAGTGG 12361 CATGGCCAGG GAGGAATCAG ATGATGAGCT CTCCCTCGGT CATGGCAGGA GGGACTCCTC 12421 CGATTCTCTC AAGTCCATCA ACCTGGCCGC GTCTGTGGAG TCAGTGCATG TGACCTTCCC 12481 GGAGCAGCAG ACAGCTGTCC GGAGGAAGCG CTCCGGCAGC AGCTCCGAGC CATCCCAGAG 12541 ATCCAGCTTT TCTTCAAACA GATCCCAAAG AGGCAGCACA AGCACCCGAA ACAGCAGTCA 12601 AAAAGGAAGC AGTGTTTTGA GTATTAAGCA AAAAGGCAAA AGGGAACTTT ATATGGAAAA 12661 GCTTCAAGAA CATTTAATCA AAGCAAAAGC CTTTACCATA AAGAAGACGC TGGAGATCTA 12721 TGTGCCCATC AAACAGTTCT TTTACAACCT CATCCACCCG GAGTATAGCG CCGTGACTGA 12781 CGTGTATGTA CTCATGTTCC TGGCTGACAC TGTGGACTTC ATCATCATTG TCTTCGGCTT 12841 TTGGGCCTTT GGGAAACACT CAGCAGCTGC AGACATCACC TCTTCACTGT CAGAGGACCA 12901 GGTCCCGGGG CCGTTTTTGG TGATGGTCCT CATTCAGTTT GGAACCATGG TGGTGGACCG 12961 AGCCCTCTAC CTCAGGAAGA CTGTACTGGG AAAGGTCATC TTCCAGGTCA TTCTTGTGTT 13021 CGGAATTCAC TTCTGGATGT TCTTCATCTT ACCTGGTGTG ACTGAGAGGA AATTCAGCCA 13081 GAACCTGGTT GCCCAGCTTT GGTACTTTGT GAAATGTGTT TACTTCGGGT TGTCTGCTTA 13141 CCAGATCCGT TGTGGCTACC CAACGCGAGT CCTGGGGAAC TTCCTCACCA AGAGCTACAA 13201 TTACGTCAAC CTCTTCTTAT TCCAAGGGTT TCGCCTCGTG CCCTTTTTGA CTGAGCTGAG 13261 GGCAGTGATG GACTGGGTGT GGACGGACAC AACTTTGAGC CTGTCCAGCT GGATCTGTGT 13321 GGAGGACATC TATGCTCACA TATTCATCCT GAAGTGTTGG CGGGAGTCGG AGAAGAGATA 13381 CCCTCAGCCA CGGGGCCAGA AGAAGAAGAA AGTGGTGAAG TATGGCATGG GAGGAATGAT 13441 CATCGTCCTG CTCATCTGCA TTGTCTGGTT TCCTCTTCTC TTCATGTCTT TGATCAAATC 13501 TGTGGCTGGG GTCATCAACC AGCCCCTGGA CGTCTCCGTC ACAATTACCC TGGGAGGGTA 13561 TCAGCCTATT TTCACAATGA GTGCCCAACA AAGCCAGTTG AAAGTTATGG ACCAGCAGAG 13621 CTTTAACAAA TTTATACAAG CTTTTTCTAG GGACACCGGT GCTATGCAAT TTCTGGAAAA 13681 TTATGAAAAA GAAGACATAA CAGTAGCAGA ACTGGAAGGA AACTCAAATT CTTTGTGGAC 13741 CATCAGCCCA CCCAGTAAGC AGAAAATGAT ACACGAACTC CTGGACCCCA ATAGTAGCTT 13801 CTCTGTTGTT TTTTCATGGA GTATTCAGAG AAACTTAAGT CTGGGTGCAA AATCGGAAAT 13861 AGCAACAGAT AAGCTTTCTT TTCCTCTTAA AAATATTACT CGAAAGAATA TCGCTAAAAT 13921 GATAGCAGGC AACAGCACAG AAAGTTCAAA AACACCAGTG ACCATAGAAA AGATTTATCC 13981 ATATTATGTG AAAGCACCTA GTGATTCTAA CTCAAAACCT ATAAAGCAAC TTTTATCTGA 14041 AAATAATTTC ATGGATATTA CCATCATTTT GTCCAGAGAC AATACAACTA AATATAACAG 14101 TGAGTGGTGG GTTCTCAACC TGACTGGAAA CAGAATATAC AATCCGAACT CTCAGGCCCT 14161 GGAACTGGTG GTCTTCAATG ACAAAGTCAG TCCCCCAAGT CTGGGGTTCC TGGCTGGCTA 14221 TGGTATTATG GGATTATATG CTTCAGTTGT CCTTGTGATT GGGAAATTTG TCCGTGAATT 14281 CTTCAGTGGG ATTTCTCACT CCATCATGTT TGAAGAGCTT CCAAATGTGG ATCGAATTTT 14341 GAAGTTGTGC ACAGATATTT TTTTAGTTCG AGAGACAGGA GAACTGGAGC TAGAAGAAGA 14401 TCTCTATGCC AAATTAATAT TCCTATATCG CTCACCAGAG ACAATGATCA AATGGACTAG 14461 AGAAAAAACA AATGACTACA AGGACGACGA TGACAAGTGA CTCGAGCCTC TAGACAGCGC 14521 TCACCGGTCG TTAACATTAA GCTAGCCTAG TGCCATTTGT TCAGTGGTTC GTAGGGCTTT 14581 CCCCCACTGT TTGGCTTTCA GTTATATGGA TGATGTGGTA TTGGGGGCCA AGTCTGTACA 14641 GCATCTTGAG TCCCTTTTTA CCGCTGTTAC CAATTTTCTT TTGTCTTTGG GTAAACCCGG 14701 GAATTCCGCC CCTCTCCCCC CCTAACGTTA CTGGCCGAAG CCGCTTGGAA TAAGGCCGGT 14761 GTGCGTTTGT CTATATGTTA TTTTCCACCA TATTGCCGTC TTTTGGCAAT GTGAGGGCCC 14821 GGAAACCTGG CCCTGTCTTC TTGACGAGCA TTCCTAGGGG TCTTTCCCCT CTCGCCAAAG 14881 GAATGCAAGG TCTGTTGAAT GTCGTGAAGG AAGCAGTTCC TCTGGAAGCT TCTTGAAGAC 14941 AAACAACGTC TGTAGCGACC CTTTGCAGGC AGCGGAACCC CCCACCTGGC GACAGGTGCC 15001 TCTGCGGCCA AAAGCCACGT GTATAAGATA CACCTGCAAA GGCGGCACAA CCCCAGTGCC 15061 ACGTTGTGAG TTGGATAGTT GTGGAAAGAG TCAAATGGCT CTCCTCAAGC GTATTCAACA 15121 AGGGGCTGAA GGATGCCCAG AAGGTACCCC ATTGTATGGG ATCTGATCTG GGGCCTCGGT 15181 GCACATGCTT TACATGTGTT TAGTCGAGGT TAAAAAAACG TCTAGGCCCC CCGAACCACG 15241 GGGACGTGGT TTTCCTTTGA AAAACACGAT GATAATATGG ATATGGCCAC AACGGTCGCC 15301 ACCATGACCG AGTACAAGCC CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCAGGGCC 15361 GTACGCACCC TCGCCGCCGC GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGATCCG 15421 GACCGCCACA TCGAGCGGGT CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC 15481 GACATCGGCA AGGTGTGGGT CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG 15541 GAGAGCGTCG AAGCGGGGGC GGTGTTCGCC GAGATCGGCC CGCGCATGGC CGAGTTGAGC 15601 GGTTCCCGGC TGGCCGCGCA GCAACAGATG GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG 15661 GAGCCCGCGT GGTTCCTGGC CACCGTCGGC GTCTCGCCCG ACCACCAGGG CAAGGGTCTG 15721 GGCAGCGCCG TCGTGCTCCC CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC 15781 CTGGAGACCT CCGCGCCCCG CAACCTCCCC TTCTACGAGC GGCTCGGCTT CACCGTCACC 15841 GCCGACGTCG AGGTGCCCGA AGGACCGCGC ACCTGGTGCA TGACCCGCAA GCCCGGTGCC 15901 TGACGCCCGC CCCACGACCC GCAGCGCCCG ACCGAAAGGA GCGCACGACC CCATGCATGT 15961 ATTCAATCTA AGCAGGCTTT CACTTTCTCG CCAACTTACA AGGCCTTTCT GTGTAAACAA 16021 TACCTGAACC TTTACCCCGT TGCCCGGCAA CGGCCAGGTC TGTGCCAAGT GTTTGCTGAC 16081 GCAACCCCCA CTGGCTGGGG CTTGGTCATG GGCCATCAGC GCATGCGTGG AACCTTTTCG 16141 GCTCCTCTGC CGATCCATAC TGCGGAACTC CTAGCCGCTT GTTTTGCTCG CAGCAGGTCT 16201 GGAGCAAACA TTATCGGGAC TGATAACTCT GTTGTCCTAT CCCGCAAATA TACATCGTTT 16261 CCATGGCTGC TAGGCTGTGC TGCCAACTGG ATCCTGCGCG GGACGTCCTT TGTTTACGTC 16321 CCGTCGGCGC TGAATCCTGC GGACGACCCT TCTCGGGGTC GCTTGGGACT CTCTCGTCCC 16381 CTTCTCCGTC TGCCGTTCCG ACCGACCACG GGGCGCACCT CTCTTTACGC GGACTCCCCG 16441 TCTGTGCCTT CTCATCTGCC GGACCGTGTG CACTTCGCTT CACCTCTGCA CGTCGCATGG 16501 AGACCACCGT GAACGCCCAC CAAATATTGC CCAAGGTCTT ACATAAGAGG ACTCTTGGAC 16561 TCTCAGCAAT GTCAACGACC GACCTTGAGG CATACTTCAA AGACTGTTTG TTTAAAGACT 16621 GGGAGGAGTT GGGGGAGGAG ATTAGGTTAA AGGTCTTTGT ACTAGGAGGC TGTAGGCATA 16681 AATTGGTCTG CGCACCAGCA CCATGGCGCA ATCACTAGAG CGGGGTACCT TTAAGACCAA 16741 TGACTTACAA GGCAGCTGTA GATCTTAGCC ACTTTTTAAA AGAAAAGGGG GGACTGGAAG 16801 GGCTAATTCA CTCCCAACGA AGACAAGATC TGCTTTTTGC TTGTACTGGG TCTCTCTGGT 16861 TAGACCAGAT CTGAGCCTGG GAGCTCTCTG GCTAACTAGG GAACCCACTG CTTAAGCCTC 16921 AATAAAGCTT GCCTTGAGTG CTTCAAGTAG TGTGTGCC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.