pLV3-CMV-AFG3L2-3×HA-CopGFP-Pu

pLV3-CMV-AFG3L2-3×HA-CopGFP-Puro plasmid

PVT97123

pLV3-CMV-AFG3L2-3×HA-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-AFG3L2(human)-3×HA-CopGFP-Puro
Alias: -
Gene lenght: 2391bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 30°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-AFG3L2-3×HA-CopGFP-Puro plasmid Description

pLV3-CMV-AFG3L2-3×HA-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-AFG3L2-3×HA-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg cgcaccgctg tttgcggctg 2341 tggggccggg gcggctgctg gccccgcggc ctacagcagc tcctcgtgcc tggcggcgtg 2401 ggcccgggcg agcagccctg cctccggacg ctttaccgat ttgttacaac tcaagcaagg 2461 gccagcagaa attctctttt gacagatata attgctgctt atcaaagatt ctgttctcga 2521 cccccaaaag gatttgaaaa atactttcct aatggaaaaa atggaaaaaa agctagtgaa 2581 cctaaagaag ttatgggaga gaaaaaagaa tcaaagccag ctgctaccac acgctcttct 2641 ggaggaggag gtggtggcgg tggaaaacga ggtggcaaga aagatgattc tcactggtgg 2701 tccaggtttc agaagggtga cattccatgg gacgacaagg atttcaggat gttcttcctc 2761 tggactgctc tgttctgggg tggagtcatg ttttacttgc tgctcaagag atccgggaga 2821 gaaatcactt ggaaggactt tgtcaataac tatctttcaa aaggagtagt agacagattg 2881 gaagtcgtca acaagcgttt tgttcgagtg acctttacac caggaaaaac tcctgttgat 2941 gggcaatacg tttggtttaa tattggcagt gtggacacct ttgaacggaa tctggaaact 3001 ttacagcagg aattgggcat agaaggagaa aatcgggtgc ctgttgtcta cattgctgaa 3061 agtgatggct cttttctgct gagcatgctg cctacggtgc tcatcatcgc cttcttgctc 3121 tacaccatca gaagagggcc tgctggcatt ggccggacag gccgagggat gggcggactc 3181 ttcagtgtcg gagaaaccac tgccaaggtc ttaaaggatg aaattgatgt gaagttcaaa 3241 gatgtggctg gctgtgagga ggccaagcta gagatcatgg aatttgtgaa tttcttgaaa 3301 aacccaaagc agtatcaaga cctaggagca aaaatcccaa agggtgccat tctcactggt 3361 cctccaggca ctgggaagac gctgctagct aaggccacag ccggagaagc caatgtcccc 3421 ttcatcaccg ttagtggatc tgagtttttg gagatgttcg ttggtgtggg ccctgctaga 3481 gtccgagact tatttgccct tgctcggaag aatgcccctt gcatcctctt catcgatgaa 3541 atcgatgcgg tgggaaggaa gagaggaaga ggcaactttg gagggcagag tgagcaggag 3601 aacacactca accagctgct ggtggagatg gatggtttta atacaacaac aaatgtcgtc 3661 attttggccg gcaccaatcg accagatatc ctggaccccg cgctgcttag gccggggcgt 3721 ttcgacaggc agatctttat tggaccacca gacataaaag gaagagcttc tattttcaaa 3781 gttcatctcc gaccgctaaa actggacagt accctggaga aggataaatt ggcaagaaaa 3841 ctggcatctt taactccagg gttttcaggt gctgatgttg ctaatgtctg taatgaagct 3901 gcgttgattg ctgcaaggca tctgtcagat tccataaatc agaaacactt tgaacaggca 3961 attgaacgag tgattggtgg cttagagaag aaaacgcagg ttctgcagcc tgaggagaag 4021 aagactgtgg cataccacga agcaggccat gcggttgccg gctggtatct ggagcacgca 4081 gacccgcttt taaaggtatc catcatccca cgtggcaaag gactaggtta tgctcagtat 4141 ttaccaaaag aacaatacct ctataccaaa gagcagctct tggataggat gtgtatgact 4201 ttaggtggtc gagtctctga agaaatcttc tttggaagaa ttacaactgg tgctcaagat 4261 gacttgagaa aagtaactca gagtgcatat gcccaaattg ttcagtttgg catgaatgaa 4321 aaggttgggc aaatctcctt tgacctccca cgtcaggggg acatggtatt ggagaaacct 4381 tacagtgaag ccactgcaag attgatagat gatgaagtac gaatacttat taatgatgct 4441 tataaaagaa cagtagctct tctcacagaa aagaaagctg acgtggagaa ggttgctctt 4501 ctgttgttag aaaaagaagt attagataag aatgatatgg ttgaactttt gggccccaga 4561 ccatttgcgg aaaaatctac ctatgaagaa tttgtggaag gcactggcag cttggatgag 4621 gacacctcac ttccagaagg ccttaaggac tggaacaagg agcgggaaaa ggagaaagag 4681 gagcccccgg gtgagaaagt tgccaacCTC GAGTACCCAT ACGACGTACC TGATTATGCA 4741 ACTTATCCTT ATGATGTACC TGATTATGCT AGCTATCCTT ATGATGTCCC AGACTACGCA 4801 TAGggatccC TGCGATATCC AGCgcggccg cGaaggatct gcgatcgctc cggtgcccgt 4861 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat 4921 tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg 4981 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac 5041 gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc 5101 tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt 5161 ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag 5221 ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg 5281 ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt 5341 ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagacgccac catggagagc 5401 gacgagagcg gcctgcccgc catggagatc gagtgccgca tcaccggcac cctgaacggc 5461 gtggagttcg agctggtggg cggcggagag ggcaccccca agcagggccg catgaccaac 5521 aagatgaaga gcaccaaagg cgccctgacc ttcagcccct acctgctgag ccacgtgatg 5581 ggctacggct tctaccactt cggcacctac cccagcggct acgagaaccc cttcctgcac 5641 gccatcaaca acggcggcta caccaacacc cgcatcgaga agtacgagga cggcggcgtg 5701 ctgcacgtga gcttcagcta ccgctacgag gccggccgcg tgatcggcga cttcaaggtg 5761 gtgggcaccg gcttccccga ggacagcgtg atcttcaccg acaagatcat ccgcagcaac 5821 gccaccgtgg agcacctgca ccccatgggc gataacgtgc tggtgggcag cttcgcccgc 5881 accttcagcc tgcgcgacgg cggctactac agcttcgtgg tggacagcca catgcacttc 5941 aagagcgcca tccaccccag catcctgcag aacgggggcc ccatgttcgc cttccgccgc 6001 gtggaggagc tgcacagcaa caccgagctg ggcatcgtgg agtaccagca cgccttcaag 6061 acccccatcg ccttcgccag atcccgcgct cagtcgtcca attctgccgt ggacggcacc 6121 gccggacccg gctccaccgg atctcgcgag ggcagaggaa gtcttctaac atgcggtgac 6181 gtggaggaga atcccggccc tatgaccgag tacaagccca cggtgcgcct cgccacccgc 6241 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg 6301 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc 6361 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg 6421 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg 6481 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg 6541 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac 6601 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc 6661 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg 6721 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg 6781 acccgcaagc ccggtgcctg aaatcaacct ctggattaca aaatttgtga aagattgact 6841 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg 6901 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg 6961 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg 7021 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg 7081 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc 7141 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg 7201 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc 7261 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct 7321 ctgcggcctc ttccgcgtct ccgccttcgc cctcagacga gtcggatctc cctttggccg 7381 cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac 7441 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaaa ataagatctg 7501 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc 7561 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg 7621 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg 7681 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca 7741 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa 7801 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 7861 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa 7921 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag 7981 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag 8041 gcctagactt ttgcagagac ggcccaaatt cgtaatcatg gtcatagctg tttcctgtgt 8101 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 8161 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt 8221 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 8281 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 8341 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 8401 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 8461 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 8521 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 8581 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 8641 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 8701 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 8761 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 8821 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 8881 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 8941 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 9001 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 9061 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 9121 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 9181 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 9241 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 9301 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 9361 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 9421 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 9481 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 9541 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 9601 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 9661 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 9721 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 9781 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 9841 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 9901 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 9961 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 10021 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 10081 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 10141 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 10201 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga 10261 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg 10321 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg 10381 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct 10441 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa 10501 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc 10561 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag 10621 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt 10681 gtaaaacgac ggccagtgcc aagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.