pLV3-CMV-YTHDF1-EF1a-CopGFP-Pu

pLV3-CMV-YTHDF1-EF1a-CopGFP-Puro plasmid

PVT97226

pLV3-CMV-YTHDF1-EF1a-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-YTHDF1(human)-EF1a-CopGFP-Puro
Alias: NM_017798.4;C20orf21; DF1
Gene lenght: 1680bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-YTHDF1-EF1a-CopGFP-Puro plasmid Description

pLV3-CMV-YTHDF1-EF1a-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-YTHDF1-EF1a-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgt cggccaccag cgtggacacc 2341 cagagaacaa aaggacaaga taataaagta caaaatggtt cgttacatca gaaggataca 2401 gttcatgaca atgactttga gccctacctt actggacagt caaatcagag taacagttac 2461 ccctcaatga gcgaccccta cctgtccagc tattacccgc cgtccattgg atttccttac 2521 tccctcaatg aggctccgtg gtctactgca ggggaccctc cgattccata cctcaccacc 2581 tacggacagc tcagtaacgg agaccatcat tttatgcacg atgctgtttt tgggcagcct 2641 gggggcctgg ggaacaacat ctatcagcac aggttcaatt ttttccctga aaaccctgcg 2701 ttctcagcat gggggacaag tgggtctcaa ggtcagcaga cccagagctc cgcgtatggg 2761 agcagctaca cctacccccc gagctccctg ggtggcacgg tggttgatgg gcagccaggc 2821 tttcacagcg acaccctcag caaggccccc gggatgaaca gcctggagca gggcatggtt 2881 ggcctgaaga ttggggacgt cagctcctcc gccgtcaaga cggtgggctc tgtcgtcagc 2941 agcgtggcac tgactggtgt cctttctggc aacggtggga caaatgtgaa catgccagtt 3001 tcaaagccga cctcgtgggc tgccattgcc agcaagcctg caaaaccaca gcctaaaatg 3061 aaaacaaaga gcgggcctgt catggggggt gggctgcccc ctccacccat aaagcataac 3121 atggacattg gcacctggga taacaagggg cctgtgccga aggccccagt cccccagcag 3181 gcaccctctc cacaggctgc cccacagccc cagcaggtgg ctcagcctct cccagcacag 3241 cccccagctt tggctcaacc gcagtatcag agccctcagc agccacccca gacccgctgg 3301 gttgccccac gcaacagaaa cgcggcgttt gggcagagcg gaggggctgg cagcgatagc 3361 aactctcctg gaaacgtcca gcctaattct gcccccagcg tcgaatccca ccccgtcctt 3421 gaaaaactga aggctgctca cagctacaac ccgaaagagt ttgagtggaa tctgaaaagc 3481 gggcgtgtgt tcatcatcaa gagctactct gaggacgaca tccaccgctc cattaagtac 3541 tccatctggt gtagcacaga gcacggcaac aagcgcctgg acagcgcctt ccgctgcatg 3601 agcagcaagg ggcccgtcta cctgctcttc agcgtcaatg ggagtgggca tttttgtggg 3661 gtggccgaga tgaagtcccc cgtggactac ggcaccagtg ccggggtctg gtctcaggac 3721 aagtggaagg ggaagtttga tgtccagtgg atttttgtta aggatgtacc caataaccag 3781 ctccggcaca tcaggctgga gaataacgac aacaaaccgg tcacaaactc ccgggacacc 3841 caggaggtgc ccttagaaaa agccaagcaa gtgctgaaaa ttatcagttc ctacaagcac 3901 acaacctcca tcttcgacga ctttgctcac tacgagaagc gccaggagga ggaggaggtg 3961 gtgcgcaagg aacggcagag tcgaaacaaa caatgaggat ccCTGCGATA TCCAGCgcgg 4021 ccgcGaagga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca 4081 gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc 4141 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga 4201 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc 4261 agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc 4321 tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc 4381 tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg 4441 gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt 4501 gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga 4561 ccggcgccta cgctagacgc caccatggag agcgacgaga gcggcctgcc cgccatggag 4621 atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt tcgagctggt gggcggcgga 4681 gagggcaccc ccaagcaggg ccgcatgacc aacaagatga agagcaccaa aggcgccctg 4741 accttcagcc cctacctgct gagccacgtg atgggctacg gcttctacca cttcggcacc 4801 taccccagcg gctacgagaa ccccttcctg cacgccatca acaacggcgg ctacaccaac 4861 acccgcatcg agaagtacga ggacggcggc gtgctgcacg tgagcttcag ctaccgctac 4921 gaggccggcc gcgtgatcgg cgacttcaag gtggtgggca ccggcttccc cgaggacagc 4981 gtgatcttca ccgacaagat catccgcagc aacgccaccg tggagcacct gcaccccatg 5041 ggcgataacg tgctggtggg cagcttcgcc cgcaccttca gcctgcgcga cggcggctac 5101 tacagcttcg tggtggacag ccacatgcac ttcaagagcg ccatccaccc cagcatcctg 5161 cagaacgggg gccccatgtt cgccttccgc cgcgtggagg agctgcacag caacaccgag 5221 ctgggcatcg tggagtacca gcacgccttc aagaccccca tcgccttcgc cagatcccgc 5281 gctcagtcgt ccaattctgc cgtggacggc accgccggac ccggctccac cggatctcgc 5341 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctatgacc 5401 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc 5461 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac 5521 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc 5581 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc 5641 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg 5701 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg 5761 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc 5821 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc 5881 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc 5941 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaaatcaa 6001 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt 6061 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct 6121 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc 6181 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg 6241 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc 6301 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc 6361 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt 6421 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca 6481 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tctccgcctt 6541 cgccctcaga cgagtcggat ctccctttgg ccgcctcccc gcctggtacc tttaagacca 6601 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa 6661 gggctaattc actcccaacg aaaataagat ctgctttttg cttgtactgg gtctctctgg 6721 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 6781 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 6841 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca 6901 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg 6961 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 7021 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 7081 tatcatgtct ggctctagct atcccgcccc taactccgcc cagttccgcc cattctccgc 7141 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc 7201 tattccagaa gtagtgagga ggcttttttg gaggcctaga cttttgcaga gacggcccaa 7261 attcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac 7321 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac 7381 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc 7441 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg 7501 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 7561 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 7621 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 7681 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 7741 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 7801 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 7861 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 7921 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 7981 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 8041 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 8101 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 8161 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 8221 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 8281 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 8341 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 8401 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 8461 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 8521 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 8581 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 8641 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 8701 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 8761 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 8821 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 8881 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 8941 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 9001 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 9061 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 9121 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 9181 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 9241 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 9301 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 9361 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 9421 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca 9481 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc 9541 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg 9601 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga 9661 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat 9721 accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc 9781 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt 9841 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.