pLenti-hPGK-Fluc-EF1a-Hyg plas

pLenti-hPGK-Fluc-EF1a-Hyg plasmid

PVT97115

pLenti-hPGK-Fluc-EF1a-Hyg plasmid Information

Plasmid name: pLenti-hPGK-Fluc-EF1a-Hyg
Alias: -
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker: Hyg
Fluorescent labeling: Fluc
Promoter: hPGK
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: EF1a-R
Reverse primer:
Copy number: High
Induction:

pLenti-hPGK-Fluc-EF1a-Hyg plasmid Description

pLenti-hPGK-Fluc-EF1a-Hyg It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLenti-hPGK-Fluc-EF1a-Hyg plasmid Sequence

1 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag 61 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg 121 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact 181 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacaa taaacgggtc 241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 421 cgcccgaaca gggacctgaa agcgaaaggg aaaccagagc tctctcgacg caggactcgg 481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt 541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg 601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa 661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg 721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca 781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa 841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa 901 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag 961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt 1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg 1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cctcaatgac 1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct 1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct 1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg 1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa 1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa 1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa 1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac 1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag 1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc 1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga 1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg 1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga 1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa 1921 ttttatcgat cacgagacta gcctcgagaa gcttgatatc gaattcccac ggggttgggg 1981 ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt 2041 tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc 2101 gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc 2161 ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca 2221 cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg 2281 cgatgggctg tggccaatag cggctgctca gcggggcgcg ccgagagcag cggccgggaa 2341 ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg 2401 tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga 2461 atcaccgacc tctctcccca gggggatccG CCACCATGGA AGATGCCAAA AACATTAAGA 2521 AGGGCCCAGC GCCATTCTAC CCACTCGAAG ACGGGACCGC CGGCGAGCAG CTGCACAAAG 2581 CCATGAAGCG CTACGCCCTG GTGCCCGGCA CCATCGCCTT TACCGACGCA CATATCGAGG 2641 TGGACATTAC CTACGCCGAG TACTTCGAGA TGAGCGTTCG GCTGGCAGAA GCTATGAAGC 2701 GCTATGGGCT GAATACAAAC CATCGGATCG TGGTGTGCAG CGAGAATAGC TTGCAGTTCT 2761 TCATGCCCGT GTTGGGTGCC CTGTTCATCG GTGTGGCTGT GGCCCCAGCT AACGACATCT 2821 ACAACGAGCG CGAGCTGCTG AACAGCATGG GCATCAGCCA GCCCACCGTC GTATTCGTGA 2881 GCAAGAAAGG GCTGCAAAAG ATCCTCAACG TGCAAAAGAA GCTACCGATC ATACAAAAGA 2941 TCATCATCAT GGATAGCAAG ACCGACTACC AGGGCTTCCA AAGCATGTAC ACCTTCGTGA 3001 CTTCCCATTT GCCACCCGGC TTCAACGAGT ACGACTTCGT GCCCGAGAGC TTCGACCGGG 3061 ACAAAACCAT CGCCCTGATC ATGAACAGTA GTGGCAGTAC CGGATTGCCC AAGGGCGTAG 3121 CCCTACCGCA CCGCACCGCT TGTGTCCGAT TCAGTCATGC CCGCGACCCC ATCTTCGGCA 3181 ACCAGATCAT CCCCGACACC GCTATCCTCA GCGTGGTGCC ATTTCACCAC GGCTTCGGCA 3241 TGTTCACCAC GCTGGGCTAC TTGATCTGCG GCTTTCGGGT CGTGCTCATG TACCGCTTCG 3301 AGGAGGAGCT ATTCTTGCGC AGCTTGCAAG ACTATAAGAT TCAATCTGCC CTGCTGGTGC 3361 CCACACTATT TAGCTTCTTC GCTAAGAGCA CTCTCATCGA CAAGTACGAC CTAAGCAACT 3421 TGCACGAGAT CGCCAGCGGC GGGGCGCCGC TCAGCAAGGA GGTAGGTGAG GCCGTGGCCA 3481 AACGCTTCCA CCTACCAGGC ATCCGCCAGG GCTACGGCCT GACAGAAACA ACCAGCGCCA 3541 TTCTGATCAC CCCCGAAGGG GACGACAAGC CTGGCGCAGT AGGCAAGGTG GTGCCCTTCT 3601 TCGAGGCTAA GGTGGTGGAC TTGGACACCG GTAAGACACT GGGTGTGAAC CAGCGCGGCG 3661 AGCTGTGCGT CCGTGGCCCC ATGATCATGA GCGGCTACGT TAACAACCCC GAGGCTACAA 3721 ACGCTCTCAT CGACAAGGAC GGCTGGCTGC ACAGCGGCGA CATCGCCTAC TGGGACGAGG 3781 ACGAGCACTT CTTCATCGTG GACCGGCTGA AGAGCCTGAT CAAATACAAG GGCTACCAGG 3841 TAGCCCCAGC CGAACTGGAG AGCATCCTGC TGCAACACCC CAACATCTTC GACGCCGGGG 3901 TCGCCGGCCT GCCCGACGAC GATGCCGGCG AGCTGCCCGC CGCAGTCGTC GTGCTGGAAC 3961 ACGGTAAAAC CATGACCGAG AAGGAGATCG TGGACTATGT GGCCAGCCAG GTTACAACCG 4021 CCAAGAAGCT GCGCGGTGGT GTTGTGTTCG TGGACGAGGT GCCTAAAGGA CTGACCGGCA 4081 AGTTGGACGC CCGCAAGATC CGCGAGATTC TCATTAAGGC CAAGAAGGGC GGCAAGATCG 4141 CCGTGTAAta gcggccgcGa aggatctgcg atcgctccgg tgcccgtcag tgggcagagc 4201 gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga acgggtgcct 4261 agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc 4321 ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca 4381 acgggtttgc cgccagaaca cagATGAAAA AGCCTGAACT CACCGCGACG TCTGTCGAGA 4441 AGTTTCTGAT CGAAAAGTTC GACAGCGTCT CCGACCTGAT GCAGCTCTCG GAGGGCGAAG 4501 AATCTCGTGC TTTCAGCTTC GATGTAGGAG GGCGTGGATA TGTCCTGCGG GTAAATAGCT 4561 GCGCCGATGG TTTCTACAAA GATCGTTATG TTTATCGGCA CTTTGCATCG GCCGCGCTCC 4621 CGATTCCGGA AGTGCTTGAC ATTGGGGAAT TTAGCGAGAG CCTGACCTAT TGCATCTCCC 4681 GCCGTGCACA GGGTGTCACG TTGCAAGACC TGCCTGAAAC CGAACTGCCC GCTGTTCTGC 4741 AGCCGGTCGC GGAGGCCATG GATGCGATCG CTGCGGCCGA TCTTAGCCAG ACGAGCGGGT 4801 TCGGCCCATT CGGACCGCAA GGAATCGGTC AATACACTAC ATGGCGTGAT TTCATATGCG 4861 CGATTGCTGA TCCCCATGTG TATCACTGGC AAACTGTGAT GGACGACACC GTCAGTGCGT 4921 CCGTCGCGCA GGCTCTCGAT GAGCTGATGC TTTGGGCCGA GGACTGCCCC GAAGTCCGGC 4981 ACCTCGTGCA CGCGGATTTC GGCTCCAACA ATGTCCTGAC GGACAATGGC CGCATAACAG 5041 CGGTCATTGA CTGGAGCGAG GCGATGTTCG GGGATTCCCA ATACGAGGTC GCCAACATCT 5101 TCTTCTGGAG GCCGTGGTTG GCTTGTATGG AGCAGCAGAC GCGCTACTTC GAGCGGAGGC 5161 ATCCGGAGCT TGCAGGATCG CCGCGGCTCC GGGCGTATAT GCTCCGCATT GGTCTTGACC 5221 AACTCTATCA GAGCTTGGTT GACGGCAATT TCGATGATGC AGCTTGGGCG CAGGGTCGAT 5281 GCGACGCAAT CGTCCGATCC GGAGCCGGGA CTGTCGGGCG TACACAAATC GCCCGCAGAA 5341 GCGCGGCCGT CTGGACCGAT GGCTGTGTAG AAGTACTCGC CGATAGTGGA AACCGACGCC 5401 CCAGCACTCG TCCGAGGGCA AAGGAATAGg tcgacaatca acctctggat tacaaaattt 5461 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg 5521 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt 5581 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg 5641 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc 5701 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg 5761 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt 5821 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc 5881 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg 5941 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga 6001 tctccctttg ggccgcctcc ccgcctggaa ttcgagctcg gtacctttaa gaccaatgac 6061 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct 6121 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga 6181 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata 6241 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta 6301 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca 6361 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt 6421 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 6481 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca 6541 tgtctggctc tagctatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 6601 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc 6661 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcgtcgagac gtacccaatt 6721 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact 6781 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct 6841 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg 6901 gcgaatggcg cgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc 6961 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt 7021 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag 7081 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt 7141 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt 7201 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt 7261 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt 7321 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttcc caggtggcac 7381 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat 7441 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag 7501 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc 7561 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc 7621 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc 7681 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc 7741 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt 7801 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt 7861 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat 7921 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct 7981 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat 8041 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc 8101 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg 8161 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc 8221 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta 8281 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc 8341 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga 8401 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat 8461 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat 8521 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa 8581 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa 8641 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt 8701 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt 8761 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata 8821 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt 8881 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac 8941 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga 9001 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg 9061 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa 9121 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat 9181 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc 9241 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga 9301 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg 9361 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta 9421 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg 9481 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg 9541 cgcaattaac cctcactaaa gggaacaaaa gctggagctg ca

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.