pLV3-CMV-MISP-3×FLAG-EF1a-CopG

pLV3-CMV-MISP-3×FLAG-EF1a-CopGFP-Neo plasmid

PVT97269

pLV3-CMV-MISP-3×FLAG-EF1a-CopGFP-Neo plasmid Information

Plasmid name: pLV3-CMV-MISP(human)-3×FLAG-EF1a-CopGFP-Neo
Alias: NM_173481.4 ;C19orf21; MISP1
Gene lenght: 2037bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-MISP-3×FLAG-EF1a-CopGFP-Neo plasmid Description

pLV3-CMV-MISP-3×FLAG-EF1a-CopGFP-Neo It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-MISP-3×FLAG-EF1a-CopGFP-Neo plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcgcca ccatggaccg cgtgaccaga taccccatcc tgggcatccc 2341 tcaggcacac cgtggcaccg gcctggtgct ggatggagac accagctaca cataccatct 2401 ggtgtgcatg ggccccgagg ccagcggctg gggccaggat gagccgcaga catggcccac 2461 tgaccacagg gcccagcagg gcgtgcagag gcagggggtg tcctacagcg tgcatgccta 2521 cactggccag ccgtccccac gggggctcca ctcggagaac agggaggatg agggttggca 2581 ggtttaccgc ctgggcgcca gggatgccca ccagggacgt ccaacatggg cactccgccc 2641 agaggacggg gaggacaagg agatgaagac ctaccgcctg gatgctgggg acgctgaccc 2701 caggaggctg tgtgacctgg agcgggagcg ctgggccgtc atccagggcc aggcagtcag 2761 gaagagcagc accgtggcca cgctccaggg cactcctgac cacggagacc ccaggacccc 2821 cggcccacct cggtccacgc ccctggagga gaacgtggtt gacagggagc agattgactt 2881 cctggcagcg agacagcagt tcctgagtct ggagcaggcg aacaaggggg cccctcatag 2941 ctccccggcc agggggaccc ctgcaggcac aaccccaggg gccagccagg cccccaaggc 3001 cttcaacaag ccccacctgg ccaacgggca cgtggttccc atcaagcccc aggtgaaggg 3061 ggtggtcagg gaagagaaca aggtgcgtgc tgtgcccacc tgggccagtg tccaagttgt 3121 ggatgaccct ggctccttgg cctcagtgga gtccccgggg acccccaagg agacgcccat 3181 cgagcgggag atccgtctgg ctcaggagcg tgaggcagac ctgcgagagc agagggggct 3241 tcggcaggca accgaccacc aggagctggt ggaaatcccc accaggccgc tgctgaccaa 3301 gctgagcctg atcacagccc cacggcggga gagagggcgc ccgtccctct acgtgcagcg 3361 ggacatagta caggagacac agcgtgagga agaccaccgg cgggagggcc tgcacgtggg 3421 ccgggcgtcc acacccgact gggtctcgga gggtccccag cccggactcc ggagagccct 3481 cagctcagat tccatcctca gcccggcccc agatgcccgt gcggccgacc cagctccaga 3541 agtgaggaag gtgaaccgca tcccacctga tgcctaccag ccgtacctga gccccgggac 3601 cccccagcta gaattctcag ccttcggagc attcggcaag cccagcagtc tctccacagc 3661 ggaggccaag gctgcgactt caccaaaggc cacgatgtcc ccgaggcatc tctcagaatc 3721 ctctggaaaa cccctgagca caaagcaaga ggcatcgaag ccccctcggg gatgcccgca 3781 agccaacagg ggtgtcgtgc ggtgggagta cttccgcctg cgtcctctgc ggttcagggc 3841 cccagacgag ccccagcagg cccaagtccc ccatgtctgg ggctgggagg tggctggggc 3901 ccctgcactg aggctgcaga agtcccagtc atctgatctg ctggaaaggg agagggagag 3961 tgtcctgcgc cgggagcaag aggtggcaga ggagcggaga aatgctctct tcccagaggt 4021 cttctcccca acgccagatg agaactctga ccagaactcc aggagctcct cccaggcatc 4081 cggcatcacg ggcagttact cggtgtctga gtctcccttc ttcagcccca tccacctaca 4141 ctcaaacgtg gcgtggacag tggaagatcc agtggacagt gctcctcccg ggcagagaaa 4201 gaaggagcaa tggtacgctg gcatcaaccc ctcggacggt atcaactcag aggtcctgga 4261 agccatacgg gtgacccgtc acaagaacgc catggcagag cgctgggaat cccgcatcta 4321 cgccagtgag gaggatgacg gatccGACTA CAAAGACCAT GACGGTGATT ATAAAGATCA 4381 TGACATCGAC TACAAGGATG ACGATGACAA GTAgcggccg cGaaggatct gcgatcgctc 4441 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg 4501 ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt 4561 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt 4621 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg 4681 ggctcgcatc tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt 4741 gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt 4801 aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag 4861 actcagccgg ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc 4921 gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagacgccac 4981 catggagagc gacgagagcg gcctgcccgc catggagatc gagtgccgca tcaccggcac 5041 cctgaacggc gtggagttcg agctggtggg cggcggagag ggcaccccca agcagggccg 5101 catgaccaac aagatgaaga gcaccaaagg cgccctgacc ttcagcccct acctgctgag 5161 ccacgtgatg ggctacggct tctaccactt cggcacctac cccagcggct acgagaaccc 5221 cttcctgcac gccatcaaca acggcggcta caccaacacc cgcatcgaga agtacgagga 5281 cggcggcgtg ctgcacgtga gcttcagcta ccgctacgag gccggccgcg tgatcggcga 5341 cttcaaggtg gtgggcaccg gcttccccga ggacagcgtg atcttcaccg acaagatcat 5401 ccgcagcaac gccaccgtgg agcacctgca ccccatgggc gataacgtgc tggtgggcag 5461 cttcgcccgc accttcagcc tgcgcgacgg cggctactac agcttcgtgg tggacagcca 5521 catgcacttc aagagcgcca tccaccccag catcctgcag aacgggggcc ccatgttcgc 5581 cttccgccgc gtggaggagc tgcacagcaa caccgagctg ggcatcgtgg agtaccagca 5641 cgccttcaag acccccatcg ccttcgccag atcccgcgct cagtcgtcca attctgccgt 5701 ggacggcacc gccggacccg gctccaccgg atctcgcgag ggcagaggaa gtcttctaac 5761 atgcggtgac gtggaggaga atcccggccc tGCCACCatg gctgaacaag atggattgca 5821 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac 5881 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt 5941 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc 6001 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg 6061 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc 6121 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc 6181 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat 6241 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc 6301 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca 6361 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga 6421 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat 6481 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc 6541 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gaGTCGACAA 6601 TCAACCTCTG GATTACAAAA TTTGTGAAAG ATTGACTGGT ATTCTTAACT ATGTTGCTCC 6661 TTTTACGCTA TGTGGATACG CTGCTTTAAT GCCTTTGTAT CATGCTATTG CTTCCCGTAT 6721 GGCTTTCATT TTCTCCTCCT TGTATAAATC CTGGTTGCTG TCTCTTTATG AGGAGTTGTG 6781 GCCCGTTGTC AGGCAACGTG GCGTGGTGTG CACTGTGTTT GCTGACGCAA CCCCCACTGG 6841 TTGGGGCATT GCCACCACCT GTCAGCTCCT TTCCGGGACT TTCGCTTTCC CCCTCCCTAT 6901 TGCCACGGCG GAACTCATCG CCGCCTGCCT TGCCCGCTGC TGGACAGGGG CTCGGCTGTT 6961 GGGCACTGAC AATTCCGTGG TGTTGTCGGG GAAATCATCG TCCTTTCCTT GGCTGCTCGC 7021 CTGTGTTGCC ACCTGGATTC TGCGCGGGAC GTCCTTCTGC TACGTCCCTT CGGCCCTCAA 7081 TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG 7141 CCTTCGCCCT CAGACGAGTC GGATCTCCCT TTGGGCCGCC TCCCCGCggt acctttaaga 7201 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg 7261 gaagggctaa ttcactccca acgaaGataa gatctgcttt ttgcttgtac tgggtctctc 7321 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag 7381 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct 7441 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt 7501 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag 7561 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 7621 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 7681 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccagttcc gcccattctc 7741 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg 7801 agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc agagacggcc 7861 caaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 7921 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct 7981 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 8041 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 8101 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 8161 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 8221 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 8281 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 8341 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 8401 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 8461 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 8521 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 8581 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 8641 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 8701 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 8761 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 8821 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 8881 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 8941 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 9001 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 9061 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 9121 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 9181 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 9241 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 9301 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 9361 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 9421 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 9481 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 9541 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 9601 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 9661 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 9721 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 9781 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 9841 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 9901 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 9961 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 10021 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta 10081 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc 10141 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc 10201 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc 10261 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa 10321 aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg 10381 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa 10441 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc 10501 tg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.