pLV3-CMV-ROR1-3×FLAG-CopGFP-Pu

pLV3-CMV-ROR1-3×FLAG-CopGFP-Puro plasmid

PVT96955

pLV3-CMV-ROR1-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-ROR1(human)-3×FLAG-CopGFP-Puro
Alias: NM_005012.4;dJ537F10.1; NTRKR1
Gene lenght: 2811bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-ROR1-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-ROR1-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-ROR1-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgc accggccgcg ccgccgcggg 2341 acgcgcccgc cgctcctggc gctgctggcc gcgctgctgc tggccgcacg cggggctgct 2401 gcccaagaaa cagagctgtc agtcagtgct gaattagtgc ctacctcatc atggaacatc 2461 tcaagtgaac tcaacaaaga ttcttacctg accctcgatg aaccaatgaa taacatcacc 2521 acgtctctgg gccagacagc agaactgcac tgcaaagtct ctgggaatcc acctcccacc 2581 atccgctggt tcaaaaatga tgctcctgtg gtccaggagc cccggaggct ctcctttcgg 2641 tccaccatct atggctctcg gctgcggatt agaaacctcg acaccacaga cacaggctac 2701 ttccagtgcg tggcaacaaa cggcaaggag gtggtttctt ccactggagt cttgtttgtc 2761 aagtttggcc cccctcccac tgcaagtcca ggatactcag atgagtatga agaagatgga 2821 ttctgtcagc catacagagg gattgcatgt gcaagattta ttggcaaccg caccgtctat 2881 atggagtctt tgcacatgca aggggaaata gaaaatcaga tcacagctgc cttcactatg 2941 attggcactt ccagtcactt atctgataag tgttctcagt tcgccattcc ttccctgtgc 3001 cactatgcct tcccgtactg cgatgaaact tcatccgtcc caaagccccg tgacttgtgt 3061 cgcgatgaat gtgaaatcct ggagaatgtc ctgtgtcaaa cagagtacat ttttgcaaga 3121 tcaaatccca tgattctgat gaggctgaaa ctgccaaact gtgaagatct cccccagcca 3181 gagagcccag aagctgcgaa ctgtatccgg attggaattc ccatggcaga tcctataaat 3241 aaaaatcaca agtgttataa cagcacaggt gtggactacc gggggaccgt cagtgtgacc 3301 aaatcagggc gccagtgcca gccatggaat tcccagtatc cccacacaca cactttcacc 3361 gcccttcgtt tcccagagct gaatggaggc cattcctact gccgcaaccc agggaatcaa 3421 aaggaagctc cctggtgctt caccttggat gaaaacttta agtctgatct gtgtgacatc 3481 ccagcgtgcg attcaaagga ttccaaggag aagaataaaa tggaaatcct gtacatacta 3541 gtgccaagtg tggccattcc cctggccatt gctttactct tcttcttcat ttgcgtctgt 3601 cggaataacc agaagtcatc gtcggcacca gtccagaggc aaccaaaaca cgtcagaggt 3661 caaaatgtag agatgtcaat gctgaatgca tataaaccca agagcaaggc taaagagcta 3721 cctctttctg ctgtacgctt tatggaagaa ttgggtgagt gtgcctttgg aaaaatctat 3781 aaaggccatc tctatctccc aggcatggac catgctcagc tggttgctat caagaccttg 3841 aaagactata acaaccccca gcaatggacg gaatttcaac aagaagcctc cctaatggca 3901 gaactgcacc accccaatat tgtctgcctt ctaggtgccg tcactcagga acaacctgtg 3961 tgcatgcttt ttgagtatat taatcagggg gatctccatg agttcctcat catgagatcc 4021 ccacactctg atgttggctg cagcagtgat gaagatggga ctgtgaaatc cagcctggac 4081 cacggagatt ttctgcacat tgcaattcag attgcagctg gcatggaata cctgtctagt 4141 cacttctttg tccacaagga ccttgcagct cgcaatattt taatcggaga gcaacttcat 4201 gtaaagattt cagacttggg gctttccaga gaaatttact ccgctgatta ctacagggtc 4261 cagagtaagt ccttgctgcc cattcgctgg atgccccctg aagccatcat gtatggcaaa 4321 ttctcttctg attcagatat ctggtccttt ggggttgtct tgtgggagat tttcagtttt 4381 ggactccagc catattatgg attcagtaac caggaagtga ttgagatggt gagaaaacgg 4441 cagctcttac catgctctga agactgccca cccagaatgt acagcctcat gacagagtgc 4501 tggaatgaga ttccttctag gagaccaaga tttaaagata ttcacgtccg gcttcggtcc 4561 tgggagggac tctcaagtca cacaagctct actactcctt cagggggaaa tgccaccaca 4621 cagacaacct ccctcagtgc cagcccagtg agtaatctca gtaaccccag atatcctaat 4681 tacatgttcc cgagccaggg tattacacca cagggccaga ttgctggttt cattggcccg 4741 ccaatacctc agaaccagcg attcattccc atcaatggat acccaatacc tcctggatat 4801 gcagcgtttc cagctgccca ctaccagcca acaggtcctc ccagagtgat tcagcactgc 4861 ccacctccca agagtcggtc cccaagcagt gccagtgggt cgactagcac tggccatgtg 4921 actagcttgc cctcatcagg atccaatcag gaagcaaata ttcctttact accacacatg 4981 tcaattccaa atcatcctgg tggaatgggt atcaccgttt ttggcaacaa atctcaaaaa 5041 ccctacaaaa ttgactcaaa gcaagcatct ttactaggag acgccaatat tcatggacac 5101 accgaatcta tgatttctgc agaactggga tccGACTACA AAGACCATGA CGGTGATTAT 5161 AAAGATCATG ACATCGACTA CAAGGATGAC GATGACAAGt agcggccgcG aaggatctgc 5221 gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg 5281 gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa 5341 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt 5401 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag 5461 cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca 5521 cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc 5581 gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc 5641 ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc 5701 tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctacgcta 5761 gacgccacca tggagagcga cgagagcggc ctgcccgcca tggagatcga gtgccgcatc 5821 accggcaccc tgaacggcgt ggagttcgag ctggtgggcg gcggagaggg cacccccaag 5881 cagggccgca tgaccaacaa gatgaagagc accaaaggcg ccctgacctt cagcccctac 5941 ctgctgagcc acgtgatggg ctacggcttc taccacttcg gcacctaccc cagcggctac 6001 gagaacccct tcctgcacgc catcaacaac ggcggctaca ccaacacccg catcgagaag 6061 tacgaggacg gcggcgtgct gcacgtgagc ttcagctacc gctacgaggc cggccgcgtg 6121 atcggcgact tcaaggtggt gggcaccggc ttccccgagg acagcgtgat cttcaccgac 6181 aagatcatcc gcagcaacgc caccgtggag cacctgcacc ccatgggcga taacgtgctg 6241 gtgggcagct tcgcccgcac cttcagcctg cgcgacggcg gctactacag cttcgtggtg 6301 gacagccaca tgcacttcaa gagcgccatc caccccagca tcctgcagaa cgggggcccc 6361 atgttcgcct tccgccgcgt ggaggagctg cacagcaaca ccgagctggg catcgtggag 6421 taccagcacg ccttcaagac ccccatcgcc ttcgccagat cccgcgctca gtcgtccaat 6481 tctgccgtgg acggcaccgc cggacccggc tccaccggat ctcgcgaggg cagaggaagt 6541 cttctaacat gcggtgacgt ggaggagaat cccggcccta tgaccgagta caagcccacg 6601 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc 6661 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc 6721 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg 6781 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg 6841 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa 6901 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc 6961 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga 7021 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac 7081 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga 7141 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa atcaacctct ggattacaaa 7201 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac 7261 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc 7321 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt 7381 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc 7441 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc 7501 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg 7561 gtgttgtcgg ggaaATCATC GTCCTTTCCT tggctgctcg cctgtgttgc cacctggatt 7621 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc 7681 cgcggcctgc tgccggctct gcggcctctt ccgcgtctTc gccttcgccc tcagacgagt 7741 cggatctccc tttggGccgc ctccccgcct ggtaccttta agaccaatga cttacaaggc 7801 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc 7861 ccaacgaaGa taagatctgc tttttgcttg tactgggtct ctctggttag accagatctg 7921 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc 7981 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct 8041 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat 8101 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc 8161 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 8221 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct 8281 ctagctaTCC CGCCCCTAAC TCCGCCCAtc ccgcccctaa ctccgcccag ttccgcccat 8341 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc 8401 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagactt ttgcagagac 8461 caaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc 8521 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta 8581 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca 8641 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc 8701 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 8761 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 8821 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 8881 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 8941 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 9001 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 9061 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 9121 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 9181 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 9241 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 9301 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt 9361 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 9421 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 9481 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 9541 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 9601 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc 9661 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta 9721 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga 9781 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg 9841 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc 9901 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat 9961 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag 10021 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat 10081 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa 10141 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa 10201 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga 10261 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg 10321 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc 10381 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg 10441 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact 10501 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat 10561 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 10621 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat 10681 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca 10741 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca 10801 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca 10861 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa 10921 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt 10981 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag 11041 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct 11101 g

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.