pLV3-CMV-CUL7-3×FLAG-Puro plas

pLV3-CMV-CUL7-3×FLAG-Puro plasmid

PVT96918

pLV3-CMV-CUL7-3×FLAG-Puro plasmid Information

Plasmid name: pLV3-CMV-CUL7(human)-3×FLAG-Puro
Alias: NM_001168370.2;3M1; CUL-7; dJ20C7.5; KIAA0076
Gene lenght: 5190bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-CUL7-3×FLAG-Puro plasmid Description

pLV3-CMV-CUL7-3×FLAG-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-CUL7-3×FLAG-Puro plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 1981 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 2041 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 2101 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 2161 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG 2221 TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT 2281 AGAGCTAGCG AATTCGCCAC Catggtggga gaactccgct acagggaatt cagggtgccc 2341 ctggggcccg gcttacatgc ctatcctgat gagctgatcc gccagcgcgt gggccatgat 2401 gggcatcctg agtaccagat ccgttggctc atcctgcggc gtggcgatga gggggacggg 2461 ggctctggcc aagtggactg caaggctgag cacatcctgc tgtggatgtc caaggatgag 2521 atctatgcca actgccacaa gatgctgggc gaggatggcc aggtcatcgg gccctcccag 2581 gagtctgcag gggaggttgg ggccctggac aaatctgtgc tggaggagat ggaaaccgac 2641 gtgaagtccc tcattcagag agcccttcgg cagctggagg agtgtgtggg cactatccct 2701 cctgctcctc tacttcacac tgtccacgtg ctcagcgcct atgccagcat tgagcccctc 2761 actggagtat tcaaggaccc aagggtcctg gacttgctca tgcacatgtt gagtagtcct 2821 gattatcaga ttcgctggag tgcaggccgg atgatacaag ccctgtcctc ccatgacgct 2881 ggtgaggggc agtgtgggga ggaagggaaa gcaggagaag ggctgggtcg gctcagggac 2941 tcacaggaca ctgtggcagg agcctctgat ctcatcagga cccggactca gatccttctg 3001 tcactgagcc aacaagaagc cattgagaaa cacctggatt ttgacagccg ctgtgctctg 3061 ctagcactgt ttgcacaggc cacgctctct gaacacccca tgtctttcga gggcattcag 3121 ctaccacagg tcccaggaag ggtgctcttc tccctggtga agcggtattt gcatgtcacc 3181 tcgctcctgg atcagctgaa cgacagtgct gcggagccag gagcccagaa cacctctgct 3241 cctgaggagt tgagtgggga gaggggtcaa ctggagctgg agttcagtat ggccatgggc 3301 accctgatct cggagctggt gcaagccatg cgctgggacc aggcctcaga cagaccaagg 3361 agctcagcac ggtcccccgg ttccatcttc cagcctcagc tggcagatgt gagcccaggg 3421 ctccccgctg cccaggctca gccctccttc aggaggtcaa gacgttttcg ccctcgttct 3481 gagttcgcaa gtggcaatac ctatgctttg tatgtgcggg acacactgca gccggggatg 3541 cgagtgcgga tgctggatga ttatgaggag atcagtgccg gggatgaggg cgagtttcgg 3601 cagagcaaca acggtgtgcc tcctgtgcag gtattttggg agtcaacagg ccgcacctat 3661 tgggtgcact ggcacatgct ggagatcttg ggctttgagg aagacattga ggacatggtt 3721 gaggctgatg agtaccaagg ggcagtggcc agtagagtcc tgggtagagc cctgcctgcc 3781 tggcgctgga ggcccatgac agaactctat gctgtgcctt atgtgctgcc tgaggatgag 3841 gacactgagg agtgtgaaca cctgaccctg gctgagtggt gggaactcct cttcttcatc 3901 aagaagctgg atggacctga ccatcaggag gttctccaga tcctccagga gaacctagat 3961 ggggagattc tggatgatga gatcctagct gaactggccg tgcccataga attggcccag 4021 gacttgctgc tgactctgcc acagcgactc aatgacagtg ccctcaggga cctgatcaac 4081 tgccatgtct acaagaagta tgggcctgaa gccctagcag ggaaccaagc ctacccatcc 4141 cttctagaag cccaagaaga tgtcctcctg ctagacgcgc aggcccaggc taaggactca 4201 gaagatgcag ccaaagtgga agcaaaagaa cccccatctc agagtcccaa cactcccctg 4261 cagcgtctgg tggagggtta tggtccagct gggaaaatcc tcctggatct agagcaagcc 4321 ctcagctcag aggggaccca ggagaacaag gtcaagccac tcctgctgca gctgcagcgg 4381 cagccgcagc ccttcctggc actgatgcag agcctggaca ctccggagac taacaggacc 4441 ctgcacctga ctgtgctgag aatcctgaag cagctggtgg acttccccga ggcactgctg 4501 ctcccctggc acgaggccgt ggatgcctgc atggcctgcc tgcggtcccc aaacactgat 4561 cgagaggtgc tccaggaact gattttcttc ctgcaccgcc tgacctcagt gagcagggac 4621 tatgccgtgg tgctgaatca gctgggagca agagacgcta tctccaaggc cctggaaaag 4681 cacctgggaa agctggagct ggctcaggag ctgcgggaca tggtgttcaa gtgtgagaag 4741 catgcccacc tctaccgcaa actcatcacc aacatcctgg gaggctgcat ccagatggtg 4801 ctgggccaga tcgaagacca cagacgaacc caccaaccca tcaacatccc tttctttgat 4861 gtgttcctca gatacctgtg ccagggctcc agtgtggaag tgaaggagga caagtgctgg 4921 gagaaggtgg aggtgtcctc caacccgcac cgggccagca agctgacgga ccacaacccc 4981 aagacctatt gggagtccaa cggcagcgcc ggctcccact acatcaccct gcacatgcgc 5041 cggggcatcc tcatcaggca actgactctg cttgtggcta gtgaggactc gagttacatg 5101 ccggcccgag tggtggtgtg cgggggtgat agcactagct ctcttcacac ggaactcaac 5161 tcggtgaatg tgatgccctc tgccagccgg gtgatcctcc tggagaacct gacccgcttc 5221 tggcccatca tccagatccg cataaagcgc tgccagcagg gtggcattga tacgcgcatt 5281 cgggggttag agatcctagg ccccaagccc acgttctggc cagtgttccg ggagcagctc 5341 tgtcgtcaca cacgcctctt ctacatggtt cgggcacagg cctggagcca ggacatggca 5401 gaggaccgca ggagcctcct gcacctgagt tctagactca acggtgctct gcgccaggag 5461 cagaattttg ctgaccgctt cctccctgat gacgaggctg cccaagctct gggcaagacc 5521 tgctgggagg ccctggtcag ccccgtggtg cagaacatca cctcccctga tgaggatggc 5581 attagccccc tgggttggct gctggaccag tacctggagt gtcaggaagc tgtcttcaac 5641 ccccagagcc gcggcccagc tttcttctcg cgggtgcgcc gtctcactca cctgctggtg 5701 catgtcgagc cctgtgaggc accccctcct gtggtggcca ctcctcggcc caaaggcaga 5761 aacagaagcc acgactggag ctccttggct acccggggcc ttccaagcag catcatgaga 5821 aacctgacgc gctgttggcg ggccgtggtg gagaagcagg tgaacaattt tctgacctca 5881 tcctggcggg atgatgactt tgtgccacgc tactgtgagc actttaatat tctgcagaac 5941 tcaagctctg aactgtttgg gcctcgggca gccttcttgc tggcgctgca aaatggctgt 6001 gcgggagcct tgctgaagct cccttttctc aaagctgccc acgtgagtga gcagttcgcc 6061 cggcacattg accagcagat ccagggcagc cggatcggtg gagcccagga aatggagagg 6121 ctggcacagc tgcagcaatg cctgcaagct gtcctgattt tctccggctt ggagatagcc 6181 accacttttg agcattatta ccagcactac atggcggacc gtctcctggg cgtggtctcg 6241 agctggctgg agggggccgt gctggagcag atcggtccct gcttccccaa ccgcctcccc 6301 cagcagatgt tgcagagcct gagcacctct aaggagctgc agcgccagtt ccacgtctac 6361 cagctccagc agctggatca ggaactcctg aagctggagg atacagagaa gaaaatacag 6421 gtgggccttg gggccagtgg caaggagcac aagagcgaga aggaagagga agctggggca 6481 gcagcagtgg tggatgtggc ggagggagag gaggaagagg aggagaatga ggacctctac 6541 tatgaagggg caatgccaga agtgtctgtg cttgtcctgt cccgacactc ctggcctgtt 6601 gcctcaatct gccacacact gaaccccaga acctgcctgc cctcctacct gaggggcact 6661 ttgaacagat actccaactt ctacaacaag agtcagagcc accctgccct tgagcgaggc 6721 tcacagaggc gactgcagtg gacgtggctg ggctgggctg agctgcagtt tgggaaccag 6781 accctgcatg tgtccaccgt gcagatgtgg ctactgctgt atctcaacga cctgaaggcg 6841 gtctctgtgg agagtctgct ggcgttctca gggctctccg cagacatgct caatcaggcg 6901 attgggcccc tcacctcttc aagaggcccc ctggaccttc acgagcaaaa ggatatacca 6961 ggaggggtcc tcaagattcg agatggcagc aaggaaccca ggtcgagatg ggacattgtg 7021 cggctcatcc cacctcagac gtacctgcaa gctgagggtg aagacggcca gaacttggag 7081 aagagacgga atcttctgaa ctgcctcatc gtccgaatcc tcaaggccca tggagatgag 7141 gggctgcaca ttgaccagct tgtctgtctg gtgctggagg cttggcagaa gggcccgtgt 7201 cctcccaggg gtttggtcag cagccttggt aaggggtctg catgcagcag cactgacgtc 7261 ctctcctgca tcctacacct cctgggcaag ggcacgctga gacgccatga cgaccggccc 7321 caggtgctgt cctatgcagt ccctgtgact gtcatggagc ctcacactga gtccctgaac 7381 ccaggctcct caggccccaa cccacccctc accttccata ccctacagat tcgctcccgg 7441 ggtgtgccct atgcctcctg cactgccacc cagagcttct ctaccttccg gGGATCCGAC 7501 TACAAAGACC ATGACGGTGA TTATAAAGAT CATGACATCG ACTACAAGGA TGACGATGAC 7561 AAGTAGCGGC CGCGAAGGAT CTGCGATCGC TCCGGTGCCC GTCAGTGGGC AGAGCGCACA 7621 TCGCCCACAG TCCCCGAGAA GTTGGGGGGA GGGGTCGGCA ATTGAACGGG TGCCTAGAGA 7681 AGGTGGCGCG GGGTAAACTG GGAAAGTGAT GTCGTGTACT GGCTCCGCCT TTTTCCCGAG 7741 GGTGGGGGAG AACCGTATAT AAGTGCAGTA GTCGCCGTGA ACGTTCTTTT TCGCAACGGG 7801 TTTGCCGCCA GAACACAGCT GAAGCTTCGA GGGGCTCGCA TCTCTCCTTC ACGCGCCCGC 7861 CGCCCTACCT GAGGCCGCCA TCCACGCCGG TTGAGTCGCG TTCTGCCGCC TCCCGCCTGT 7921 GGTGCCTCCT GAACTGCGTC CGCCGTCTAG GTAAGTTTAA AGCTCAGGTC GAGACCGGGC 7981 CTTTGTCCGG CGCTCCCTTG GAGCCTACCT AGACTCAGCC GGCTCTCCAC GCTTTGCCTG 8041 ACCCTGCTTG CTCAACTCTA CGTCTTTGTT TCGTTTTCTG TTCTGCGCCG TTACAGATCC 8101 AAGCTGTGAC CGGCGCCTAC GCTAGATGAC CGAGTACAAG CCCACGGTGC GCCTCGCCAC 8161 CCGCGACGAC GTCCCCCGGG CCGTACGCAC CCTCGCCGCC GCGTTCGCCG ACTACCCCGC 8221 CACGCGCCAC ACCGTCGACC CGGACCGCCA CATCGAGCGG GTCACCGAGC TGCAAGAACT 8281 CTTCCTCACG CGCGTCGGGC TCGACATCGG CAAGGTGTGG GTCGCGGACG ACGGCGCCGC 8341 GGTGGCGGTC TGGACCACGC CGGAGAGCGT CGAAGCGGGG GCGGTGTTCG CCGAGATCGG 8401 CCCGCGCATG GCCGAGTTGA GCGGTTCCCG GCTGGCCGCG CAGCAACAGA TGGAAGGCCT 8461 CCTGGCGCCG CACCGGCCCA AGGAGCCCGC GTGGTTCCTG GCCACCGTCG GCGTCTCGCC 8521 CGACCACCAG GGCAAGGGTC TGGGCAGCGC CGTCGTGCTC CCCGGAGTGG AGGCGGCCGA 8581 GCGCGCCGGG GTGCCCGCCT TCCTGGAGAC CTCCGCGCCC CGCAACCTCC CCTTCTACGA 8641 GCGGCTCGGC TTCACCGTCA CCGCCGACGT CGAGGTGCCC GAAGGACCGC GCACCTGGTG 8701 CATGACCCGC AAGCCCGGTG CCTGAGTCGA CAATCAACCT CTGGATTACA AAATTTGTGA 8761 AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT 8821 AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT CCTTGTATAA 8881 ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT 8941 GTGCACTGTG TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA CCTGTCAGCT 9001 CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA TCGCCGCCTG 9061 CCTTGCCCGC TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG TGGTGTTGTC 9121 GGGGAAATCA TCGTCCTTTC CTTGGCTGCT CGCCTGTGTT GCCACCTGGA TTCTGCGCGG 9181 GACGTCCTTC TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT CCCGCGGCCT 9241 GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC CCTCAGACGA GTCGGATCTC 9301 CCTTTGGGCC GCCTCCCCGC CTGGTACCTT TAAGACCAAT GACTTACAAG GCAGCTGTAG 9361 ATCTTAGCCA CTTTTTAAAA GAAAAGGGGG GACTGGAAGG GCTAATTCAC TCCCAACGAA 9421 AATAAGATCT GCTTTTTGCT TGTACTGGGT CTCTCTGGTT AGACCAGATC TGAGCCTGGG 9481 AGCTCTCTGG CTAACTAGGG AACCCACTGC TTAAGCCTCA ATAAAGCTTG CCTTGAGTGC 9541 TTCAAGTAGT GTGTGCCCGT CTGTTGTGTG ACTCTGGTAA CTAGAGATCC CTCAGACCCT 9601 TTTAGTCAGT GTGGAAAATC TCTAGCAGTA GTAGTTCATG TCATCTTATT ATTCAGTATT 9661 TATAACTTGC AAAGAAATGA ATATCAGAGA GTGAGAGGAA CTTGTTTATT GCAGCTTATA 9721 ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC 9781 ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGG CTCTAGCTAT 9841 CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT 9901 TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG 9961 CTTTTTTGGA GGCCTAGACT TTTGCAGAGA CGGCCCAAAT TCGTAATCAT GGTCATAGCT 10021 GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT 10081 AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC 10141 ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG 10201 CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT 10261 GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 10321 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 10381 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 10441 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 10501 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 10561 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 10621 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 10681 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 10741 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 10801 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT 10861 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 10921 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 10981 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 11041 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 11101 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC 11161 TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT 11221 TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT 11281 ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT 11341 ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC 11401 CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA 11461 TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG 11521 TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT 11581 GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC 11641 AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT 11701 AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG 11761 GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC 11821 TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC 11881 GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT 11941 TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG 12001 AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG 12061 CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA 12121 ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCT AAGAAACCAT 12181 TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG AGGCCCTTTC GTCTCGCGCG 12241 TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG 12301 TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG 12361 GTGTCGGGGC TGGCTTAACT ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATAT 12421 GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGC GCCATTCGCC 12481 ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC TATTACGCCA 12541 GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG GTAACGCCAG GGTTTTCCCA 12601 GTCACGACGT TGTAAAACGA CGGCCAGTGC CAAGCTG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.