pLV3-CMV-PEX19-3×HA-CopGFP-Pur

pLV3-CMV-PEX19-3×HA-CopGFP-Puro plasmid

PVT96898

pLV3-CMV-PEX19-3×HA-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-PEX19(human)-3×HA-CopGFP-Puro
Alias: NM_002857.4;D1S2223E; HK33; PBD12A; PMP1; PMPI; PXF;PXMP1
Gene lenght: 897bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-PEX19-3×HA-CopGFP-Puro plasmid Description

pLV3-CMV-PEX19-3×HA-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-PEX19-3×HA-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg ccgccgctga ggaaggctgt 2341 agtgtcgggg ccgaagcgga cagggaattg gaggagcttc tggaaagtgc tcttgatgat 2401 ttcgataaag ccaaaccctc cccagcaccc ccttctacca ccacggcccc tgatgcttcg 2461 gggccccaga agagatcgcc aggagacact gccaaagatg ccctcttcgc ttcccaagag 2521 aagtttttcc aggaactatt cgacagtgaa ctggcttccc aagccactgc ggagttcgag 2581 aaggcaatga aggagttggc tgaggaagaa ccccacctgg tggagcagtt ccaaaagctc 2641 tcagaggctg cagggagagt gggcagtgat atgacctccc aacaagaatt cacttcttgc 2701 ctaaaggaaa cactaagtgg attagccaaa aatgccactg accttcagaa ctccagcatg 2761 tcggaagaag agctgaccaa ggccatggag gggctaggca tggacgaagg ggatggggaa 2821 gggaacatcc tccccatcat gcagagtatt atgcagaacc tactctccaa ggatgtgctg 2881 tacccatcac tgaaggagat cacagaaaag tatccagaat ggttgcagag tcatcgggaa 2941 tctctacctc cagagcagtt tgaaaaatat caggagcagc acagcgtcat gtgcaaaata 3001 tgtgagcagt ttgaggcaga gacccccaca gacagtgaaa ccactcaaaa ggctcgtttt 3061 gagatggtgc tggatcttat gcagcagcta caagatttag gccatcctcc aaaagagctg 3121 gctggagaga tgcctcctgg cctcaacttt gacctggatg ccctcaatct ttcgggccca 3181 ccaggtgcca gtggtgaaca gtgtctgatc atgCTCGAGT ACCCATACGA CGTACCTGAT 3241 TATGCAACTT ATCCTTATGA TGTACCTGAT TATGCTAGCT ATCCTTATGA TGTCCCAGAC 3301 TACGCATAGg gatccCTGCG ATATCCAGCg cggccgcGaa ggatctgcga tcgctccggt 3361 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc 3421 ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg 3481 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc 3541 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct 3601 cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt 3661 cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt 3721 ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc 3781 agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt 3841 tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga cgccaccatg 3901 gagagcgacg agagcggcct gcccgccatg gagatcgagt gccgcatcac cggcaccctg 3961 aacggcgtgg agttcgagct ggtgggcggc ggagagggca cccccaagca gggccgcatg 4021 accaacaaga tgaagagcac caaaggcgcc ctgaccttca gcccctacct gctgagccac 4081 gtgatgggct acggcttcta ccacttcggc acctacccca gcggctacga gaaccccttc 4141 ctgcacgcca tcaacaacgg cggctacacc aacacccgca tcgagaagta cgaggacggc 4201 ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg gccgcgtgat cggcgacttc 4261 aaggtggtgg gcaccggctt ccccgaggac agcgtgatct tcaccgacaa gatcatccgc 4321 agcaacgcca ccgtggagca cctgcacccc atgggcgata acgtgctggt gggcagcttc 4381 gcccgcacct tcagcctgcg cgacggcggc tactacagct tcgtggtgga cagccacatg 4441 cacttcaaga gcgccatcca ccccagcatc ctgcagaacg ggggccccat gttcgccttc 4501 cgccgcgtgg aggagctgca cagcaacacc gagctgggca tcgtggagta ccagcacgcc 4561 ttcaagaccc ccatcgcctt cgccagatcc cgcgctcagt cgtccaattc tgccgtggac 4621 ggcaccgccg gacccggctc caccggatct cgcgagggca gaggaagtct tctaacatgc 4681 ggtgacgtgg aggagaatcc cggccctatg accgagtaca agcccacggt gcgcctcgcc 4741 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc 4801 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa 4861 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc 4921 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc 4981 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc 5041 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg 5101 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc 5161 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac 5221 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg 5281 tgcatgaccc gcaagcccgg tgcctgaaat caacctctgg attacaaaat ttgtgaaaga 5341 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg 5401 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc 5461 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc 5521 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt 5581 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt 5641 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg 5701 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg 5761 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg 5821 ccggctctgc ggcctcttcc gcgtctccgc cttcgccctc agacgagtcg gatctccctt 5881 tggccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc tgtagatctt 5941 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaaaataa 6001 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc 6061 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa 6121 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag 6181 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa 6241 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt 6301 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct 6361 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc 6421 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 6481 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt 6541 ttggaggcct agacttttgc agagacggcc caaattcgta atcatggtca tagctgtttc 6601 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt 6661 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc 6721 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg 6781 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct 6841 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 6901 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 6961 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 7021 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 7081 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 7141 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 7201 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 7261 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 7321 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 7381 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg 7441 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 7501 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 7561 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 7621 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 7681 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 7741 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 7801 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 7861 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 7921 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 7981 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 8041 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 8101 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 8161 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 8221 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 8281 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 8341 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 8401 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 8461 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 8521 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 8581 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 8641 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 8701 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta 8761 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg 8821 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt 8881 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc 8941 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt 9001 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca 9061 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg 9121 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac 9181 gacgttgtaa aacgacggcc agtgccaagc tg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.