pLV3-CMV-CDX2-3×FLAG-CopGFP-Pu

pLV3-CMV-CDX2-3×FLAG-CopGFP-Puro plasmid

PVT96850

pLV3-CMV-CDX2-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-CDX2(human)-3×FLAG-CopGFP-Puro
Alias: NM_001265.6;CDX-3; CDX2/AS; CDX3
Gene lenght: 939bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-CDX2-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-CDX2-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-CDX2-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgt acgtgagcta cctcctggac 2341 aaggacgtga gcatgtaccc tagctccgtg cgccactctg gcggcctcaa cctggcgccg 2401 cagaacttcg tcagcccccc gcagtacccg gactacggcg gttaccacgt ggcggccgca 2461 gctgcagcgg cagcgaactt ggacagcgcg cagtccccgg ggccatcctg gccggcagcg 2521 tatggcgccc cactccggga ggactggaat ggctacgcgc ccggaggcgc cgcggccgcc 2581 gccaacgccg tggctcacgg cctcaacggt ggctccccgg ccgcagccat gggctacagc 2641 agccccgcag actaccatcc gcaccaccac ccgcatcacc acccgcacca cccggccgcc 2701 gcgccttcct gcgcttctgg gctgctgcaa acgctcaacc ccggccctcc tgggcccgcc 2761 gccaccgctg ccgccgagca gctgtctccc ggcggccagc ggcggaacct gtgcgagtgg 2821 atgcggaagc cggcgcagca gtccctcggc agccaagtga aaaccaggac gaaagacaaa 2881 tatcgagtgg tgtacacgga ccaccagcgg ctggagctgg agaaggagtt tcactacagt 2941 cgctacatca ccatccggag gaaagccgag ctagccgcca cgctggggct ctctgagagg 3001 caggttaaaa tctggtttca gaaccgcaga gcaaaggaga ggaaaatcaa caagaagaag 3061 ttgcagcagc aacagcagca gcagccacca cagccgcctc cgccgccacc acagcctccc 3121 cagcctcagc caggtcctct gagaagtgtc ccagagccct tgagtccggt gtcttccctg 3181 caagcctcag tgcctggctc tgtccctggg gttctggggc caactggggg ggtgctaaac 3241 cccaccgtca cccagggatc cGACTACAAA GACCATGACG GTGATTATAA AGATCATGAC 3301 ATCGACTACA AGGATGACGA TGACAAGtag cggccgcGaa ggatctgcga tcgctccggt 3361 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc 3421 ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg 3481 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc 3541 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct 3601 cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt 3661 cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt 3721 ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc 3781 agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt 3841 tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga cgccaccatg 3901 gagagcgacg agagcggcct gcccgccatg gagatcgagt gccgcatcac cggcaccctg 3961 aacggcgtgg agttcgagct ggtgggcggc ggagagggca cccccaagca gggccgcatg 4021 accaacaaga tgaagagcac caaaggcgcc ctgaccttca gcccctacct gctgagccac 4081 gtgatgggct acggcttcta ccacttcggc acctacccca gcggctacga gaaccccttc 4141 ctgcacgcca tcaacaacgg cggctacacc aacacccgca tcgagaagta cgaggacggc 4201 ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg gccgcgtgat cggcgacttc 4261 aaggtggtgg gcaccggctt ccccgaggac agcgtgatct tcaccgacaa gatcatccgc 4321 agcaacgcca ccgtggagca cctgcacccc atgggcgata acgtgctggt gggcagcttc 4381 gcccgcacct tcagcctgcg cgacggcggc tactacagct tcgtggtgga cagccacatg 4441 cacttcaaga gcgccatcca ccccagcatc ctgcagaacg ggggccccat gttcgccttc 4501 cgccgcgtgg aggagctgca cagcaacacc gagctgggca tcgtggagta ccagcacgcc 4561 ttcaagaccc ccatcgcctt cgccagatcc cgcgctcagt cgtccaattc tgccgtggac 4621 ggcaccgccg gacccggctc caccggatct cgcgagggca gaggaagtct tctaacatgc 4681 ggtgacgtgg aggagaatcc cggccctatg accgagtaca agcccacggt gcgcctcgcc 4741 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc 4801 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa 4861 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc 4921 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc 4981 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc 5041 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg 5101 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc 5161 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac 5221 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg 5281 tgcatgaccc gcaagcccgg tgcctgaaat caacctctgg attacaaaat ttgtgaaaga 5341 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg 5401 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc 5461 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc 5521 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt 5581 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt 5641 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg 5701 aaATCATCGT CCTTTCCTtg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg 5761 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg 5821 ccggctctgc ggcctcttcc gcgtctTcgc cttcgccctc agacgagtcg gatctccctt 5881 tggGccgcct ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct 5941 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaaGata 6001 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct 6061 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca 6121 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta 6181 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata 6241 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg 6301 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 6361 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctaTCCCG 6421 CCCCTAACTC CGCCCAtccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 6481 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc 6541 cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacca aattcgtaat 6601 catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg 6661 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat 6721 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg 6781 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct 6841 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 6901 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 6961 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 7021 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 7081 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 7141 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 7201 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 7261 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 7321 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 7381 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 7441 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 7501 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 7561 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 7621 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 7681 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 7741 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 7801 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 7861 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 7921 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 7981 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 8041 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 8101 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 8161 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 8221 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 8281 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 8341 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 8401 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 8461 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 8521 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 8581 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 8641 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 8701 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt 8761 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt 8821 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 8881 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 8941 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag 9001 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag 9061 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc 9121 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc 9181 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.