pLV3-CMV-DVL3-3×FLAG-mCherry-P

pLV3-CMV-DVL3-3×FLAG-mCherry-Puro plasmid

PVT96848

pLV3-CMV-DVL3-3×FLAG-mCherry-Puro plasmid Information

Plasmid name: pLV3-CMV-DVL3(human)-3×FLAG-mCherry-Puro
Alias: NM_004423.4;DRS3
Gene lenght: 2148bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-DVL3-3×FLAG-mCherry-Puro plasmid Description

pLV3-CMV-DVL3-3×FLAG-mCherry-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-DVL3-3×FLAG-mCherry-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcGCCA CCatgggcga gaccaagatc atctaccact tggatgggca 2341 ggagacgccg taccttgtga agctgcccct gcccgccgag cgcgtcacct tggcggactt 2401 taagggcgtt ttgcagcgac ccagctataa gttcttcttc aagtctatgg acgacgattt 2461 cggagtggtg aaggaggaga tctcggatga caatgccaag ctaccatgct tcaatggccg 2521 ggtggtgtcc tggctggtgt cagctgaggg ctcacaccca gacccagccc ccttctgtgc 2581 tgataaccca tcggagctgc caccacctat ggagcgcacg ggaggcatcg gggactcccg 2641 acccccatcc ttccaccctc atgctggtgg gggcagccag gagaacctgg acaatgacac 2701 agagacggac tctttggtgt ctgcccagcg agagcggcca cgccggaggg atggcccaga 2761 gcatgcaacc cggctaaatg gaactgcgaa gggggaacgg cggcgagaac cagggggtta 2821 tgatagctca tccaccctta tgagcagtga gctggagacc accagcttct ttgactcaga 2881 tgaggatgac tccaccagca ggttcagcag ctccacagaa cagagcagtg cctcacgcct 2941 gatgagaaga cacaagcggc ggcggcggaa gcagaaggtt tctcggattg agcggtcctc 3001 gtccttcagc agcatcacgg actccaccat gtcactcaac atcatcacgg tcactctcaa 3061 catggaaaaa tataacttct tgggcatctc cattgtgggc caaagcaacg agcgtggtga 3121 cggcggcatc tacattggct ctatcatgaa gggtggggcc gtggctgctg atggacgcat 3181 cgagccagga gatatgttgt tacaggtaaa cgagatcaac tttgagaaca tgagtaatga 3241 cgatgcagtc cgggtactgc gggagattgt gcacaaaccg gggcccatca ccctgactgt 3301 agccaagtgc tgggacccaa gtccacgtgg ttgcttcaca ttgcccagga gcgagcccat 3361 ccggcccatt gaccctgcgg cctgggtctc ccacactgca gccatgaccg gcaccttccc 3421 tgcatacggc atgagcccct ccctgagcac catcacctcc accagctcct ccatcaccag 3481 ttccatccct gacacagagc gcctagacga cttccacttg tccatccaca gtgacatggc 3541 tgccatcgta aaagccatgg cctcccctga atcagggttg gaggtccgtg accgcatgtg 3601 gctcaagatt accatcccta atgctttcat cggctcagat gtggtggact ggctgtacca 3661 caatgtggaa ggcttcacgg accggaggga ggcccgcaag tatgccagca acctgctgaa 3721 agctggcttc atccgccata ccgtcaacaa gatcaccttc tccgagcagt gctactacat 3781 cttcggtgac ctctgcggca acatggccaa cctgtctctc cacgatcacg atggctccag 3841 tggcgcctct gaccaggaca cactggcccc tttgccgcac ccgggggccg ccccttggcc 3901 catggctttc ccgtaccagt acccgccacc cccgcaccca tacaacccgc acccgggctt 3961 cccggagctg ggctacagct acggcggggg cagcgccagc agtcagcaca gcgaaggcag 4021 tcggagcagt ggctccaacc gtagcggcag cgatcggagg aaggagaagg acccgaaggc 4081 cggggactcc aagtccgggg gcagcggcag cgaatcggac cacaccacac gcagcagcct 4141 gcgggggccg cgggagcggg cgcccagcga gcgctcaggg ccggcggcca gcgagcacag 4201 ccaccgcagc caccattccc tggccagcag ccttcgcagc caccacacac acccgagcta 4261 cggtcctccc ggagtgcccc ctctctacgg cccccccatg ctgatgatgc ccccgccgcc 4321 cgcggccatg gggcccccag gagcccctcc gggccgcgac ctggcctcag tgcccccgga 4381 actgaccgcc agcagacagt ccttccgcat ggccatggga aaccccagtg agttctttgt 4441 ggatgtgatg ggatccGACT ACAAAGACCA TGACGGTGAT TATAAAGATC ATGACATCGA 4501 CTACAAGGAT GACGATGACA AGtagcggcc gcGaaggatc tgcgatcgct ccggtgcccg 4561 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa 4621 ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg 4681 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa 4741 cgttcttttt cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat 4801 ctctccttca cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt 4861 tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa 4921 gctcaggtcg agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg 4981 gctctccacg ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt 5041 tctgcgccgt tacagatcca agctgtgacc ggcgcctacg ctagacgcca ccatggtgag 5101 caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat 5161 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta 5221 cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg 5281 ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga 5341 catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa 5401 cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt 5461 catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa 5521 gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa 5581 gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa 5641 gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa 5701 gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga 5761 gggccgccac tccaccggcg gcatggacga gctgtacaag ggatctcgcg agggcagagg 5821 aagtcttcta acatgcggtg acgtggagga gaatcccggc cctatgaccg agtacaagcc 5881 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc 5941 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt 6001 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt 6061 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc 6121 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca 6181 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc 6241 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc 6301 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg 6361 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga 6421 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgaaatcaac ctctggatta 6481 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg 6541 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc 6601 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca 6661 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac 6721 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact 6781 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc 6841 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg 6901 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc 6961 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt ctccgccttc gccctcagac 7021 gagtcggatc tccctttggc cgcctccccg cctggtacct ttaagaccaa tgacttacaa 7081 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca 7141 ctcccaacga aaataagatc tgctttttgc ttgtactggg tctctctggt tagaccagat 7201 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt 7261 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc 7321 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat 7381 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat 7441 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 7501 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg 7561 gctctagcta tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 7621 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag 7681 tagtgaggag gcttttttgg aggcctagac ttttgcagag acggcccaaa ttcgtaatca 7741 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga 7801 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt 7861 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga 7921 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc 7981 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 8041 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 8101 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 8161 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 8221 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 8281 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 8341 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 8401 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 8461 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 8521 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 8581 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 8641 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 8701 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 8761 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 8821 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 8881 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 8941 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 9001 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 9061 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 9121 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 9181 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc 9241 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 9301 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 9361 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 9421 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 9481 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca 9541 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 9601 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 9661 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 9721 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 9781 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 9841 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc 9901 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt 9961 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg 10021 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg 10081 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga 10141 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg 10201 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg 10261 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca 10321 gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.