pLV3-CMV-Pik3r1-3×FLAG-CopGFP-

pLV3-CMV-Pik3r1-3×FLAG-CopGFP-Puro plasmid

PVT96829

pLV3-CMV-Pik3r1-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Pik3r1(rat)-3×FLAG-CopGFP-Puro
Alias: NM_013005.3;PI3KA
Gene lenght: 2172bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Rat
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Pik3r1-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Pik3r1-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Pik3r1-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatga gcgcagaggg gtaccagtac 2341 agagcgctgt atgactacaa aaaggaacga gaggaagaca tcgacctaca cttgggggac 2401 atcctgactg tgaataaagg ctccttagtg gcacttggat tcagtgatgg ccaggaagcc 2461 cggccagaag atattggctg gttaaatggt tacaatgaaa ctactgggga gaggggagac 2521 tttccaggaa cttatgttga gtacattgga aggaaaagaa tttcaccccc cactcctaag 2581 ccacggcccc ctcgacctct tcctgtagca cccggttctt caaaaactga agcagacact 2641 gagcaaccag tgttgaccct tcctgatctg gccgagcagt ttgcccctcc tgatgttgcc 2701 ccgcctctcc ttataaagct cctggaagcc attgagaaga aaggactgga atgttcaact 2761 ctatacagaa cacagagctc cagcaaccct gcagagttac gacagcttct tgattgtgat 2821 gccgcctcag tggacttgga tgtgttcgat gtacacgttt tagcagatgc tttcaaacgc 2881 tatctcgccg acttaccaaa tcctgtcatt ccagtagctg tttacaatga gatgatgtct 2941 ttagcccaag aagtaccaag ctccgaagac tacatccagc tgttgaagaa gctcattagg 3001 tcgcctaata tacctcatca gtattggctt acgctccagt atttgctcaa gcacttcttc 3061 aagctctctc aagcctccag caagaacctt ctgaatgcaa gagccctctc tgaaattttc 3121 agccacgtgc ttttcagatt cccagcagcc agctctgata atactgaaca cctcataaaa 3181 gcggtagagc ttttaatctc aacggagtgg agtgagcgac agccagcacc agcactgccc 3241 cctaaaccac ccaagcccac ttctatagcc aacaacagca tgaacaacaa tatgtcctta 3301 caggatgctg aatggtactg gggagatatc tcaagggaag aagtaaatga aaaactccga 3361 gacactgctg atgggacctt tctggtacga gatgcatcca ctaaaatgca cggcgactac 3421 actctcacac taaggaaagg aggaaataac aaattaatca aaatctttca ccgagatggg 3481 aaatacggct tctctgatcc attaaccttc aactctgtgg ttgaattaat aaaccactac 3541 cggaatgagt ccttagctca gtacaatccc aagctggatg tgaagttact ctacccagtg 3601 tctaaatacc agcaggatca agttgtcaaa gaagataata ttgaagctgt agggaaaaaa 3661 ttacatgaat ataatactca atttcaagaa aaaagtcggg aatatgatag attatatgag 3721 gaatacactc gtacttccca ggaaatacag atgaaaagaa cagctatcga agcatttaat 3781 gaaaccataa aaatattcga agagcagtgc caaacccagg agcggtacag caaagaatac 3841 atagagaagt ttaaacgcga aggcaacgag aaggaaattc aaaggataat gcataatcac 3901 gataagctga agtctcggat cagcgagatc atcgacagca ggaggagact ggaggaagac 3961 ttgaagaagc aggcagccga gtatcgcgag atcgacaagc gcatgaatag cattaagccg 4021 gacctcattc agctgagaaa gacaagagat caatacttga tgtggctgac gcagaaaggg 4081 gtccggcaga agaagctgaa cgaatggttg ggaaacgaaa acacagaaga ccaatactca 4141 ctggtagaag atgacgagga tttgccccac catgatgaga agacgtggaa tgtggggagc 4201 agcaaccgaa acaaagccga gaacctattg cgagggaaac gagatggcac tttcctggtc 4261 cgggagagca gtaagcaggg ctgctatgcc tgctctgtag tggtagatgg cgaagtcaaa 4321 cattgcgtca tcaacaagac tgccaccggc tatggcttcg ccgagcccta caacctgtac 4381 agctccctga aagagctggt gctacattac caacacacct ccctggtgca gcacaatgac 4441 tccctcaatg tcacactagc atacccagta tatgcacaac agaggcgagg atccGACTAC 4501 AAAGACCATG ACGGTGATTA TAAAGATCAT GACATCGACT ACAAGGATGA CGATGACAAG 4561 tagcggccgc Gaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg 4621 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg 4681 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt 4741 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt 4801 gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc 4861 cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt 4921 gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt 4981 tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc 5041 ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag 5101 ctgtgaccgg cgcctacgct agacgccacc atggagagcg acgagagcgg cctgcccgcc 5161 atggagatcg agtgccgcat caccggcacc ctgaacggcg tggagttcga gctggtgggc 5221 ggcggagagg gcacccccaa gcagggccgc atgaccaaca agatgaagag caccaaaggc 5281 gccctgacct tcagccccta cctgctgagc cacgtgatgg gctacggctt ctaccacttc 5341 ggcacctacc ccagcggcta cgagaacccc ttcctgcacg ccatcaacaa cggcggctac 5401 accaacaccc gcatcgagaa gtacgaggac ggcggcgtgc tgcacgtgag cttcagctac 5461 cgctacgagg ccggccgcgt gatcggcgac ttcaaggtgg tgggcaccgg cttccccgag 5521 gacagcgtga tcttcaccga caagatcatc cgcagcaacg ccaccgtgga gcacctgcac 5581 cccatgggcg ataacgtgct ggtgggcagc ttcgcccgca ccttcagcct gcgcgacggc 5641 ggctactaca gcttcgtggt ggacagccac atgcacttca agagcgccat ccaccccagc 5701 atcctgcaga acgggggccc catgttcgcc ttccgccgcg tggaggagct gcacagcaac 5761 accgagctgg gcatcgtgga gtaccagcac gccttcaaga cccccatcgc cttcgccaga 5821 tcccgcgctc agtcgtccaa ttctgccgtg gacggcaccg ccggacccgg ctccaccgga 5881 tctcgcgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct 5941 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta 6001 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac 6061 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac 6121 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag 6181 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt 6241 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag 6301 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc 6361 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg 6421 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc 6481 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga 6541 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct 6601 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt 6661 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg 6721 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact 6781 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct 6841 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg 6901 ttgggcactg acaattccgt ggtgttgtcg gggaaATCAT CGTCCTTTCC Ttggctgctc 6961 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc 7021 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctT 7081 cgccttcgcc ctcagacgag tcggatctcc ctttggGccg cctccccgcc tggtaccttt 7141 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg 7201 actggaaggg ctaattcact cccaacgaaG ataagatctg ctttttgctt gtactgggtc 7261 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 7321 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 7381 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag 7441 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag 7501 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 7561 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 7621 tgtatcttat catgtctggc tctagctaTC CCGCCCCTAA CTCCGCCCAt cccgccccta 7681 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca 7741 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga 7801 ggcctagact tttgcagaga ccaaattcgt aatcatgtca tagctgtttc ctgtgtgaaa 7861 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 7921 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 7981 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 8041 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 8101 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 8161 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 8221 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 8281 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 8341 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 8401 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 8461 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 8521 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 8581 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 8641 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc 8701 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 8761 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 8821 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 8881 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 8941 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 9001 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 9061 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 9121 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 9181 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 9241 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 9301 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 9361 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 9421 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 9481 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 9541 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 9601 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 9661 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 9721 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 9781 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 9841 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 9901 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 9961 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt 10021 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg 10081 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc 10141 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct 10201 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc 10261 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa 10321 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg 10381 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa 10441 aacgacggcc agtgccaagc tg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.