pCMV-TIGIT-3×HA-mCherry-Neo pl

pCMV-TIGIT-3×HA-mCherry-Neo plasmid

PVT96774

pCMV-TIGIT-3×HA-mCherry-Neo plasmid Information

Plasmid name: pCMV-TIGIT(human)-3×HA-mCherry-Neo
Alias: NM_173799.4;VSIG9; VSTM3; WUCAM
Gene lenght: 732bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-TIGIT-3×HA-mCherry-Neo plasmid Description

pCMV-TIGIT-3×HA-mCherry-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-TIGIT-3×HA-mCherry-Neo plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagGC CACCatgcgc tggtgtctcc tcctgatctg ggcccagggg 661 ctgaggcagg ctcccctcgc ctcaggaatg atgacaggca caatagaaac aacggggaac 721 atttctgcag agaaaggtgg ctctatcatc ttacaatgtc acctctcctc caccacggca 781 caagtgaccc aggtcaactg ggagcagcag gaccagcttc tggccatttg taatgctgac 841 ttggggtggc acatctcccc atccttcaag gatcgagtgg ccccaggtcc cggcctgggc 901 ctcaccctcc agtcgctgac cgtgaacgat acaggggagt acttctgcat ctatcacacc 961 taccctgatg ggacgtacac tgggagaatc ttcctggagg tcctagaaag ctcagtggct 1021 gagcacggtg ccaggttcca gattccattg cttggagcca tggccgcgac gctggtggtc 1081 atctgcacag cagtcatcgt ggtggtcgcg ttgactagaa agaagaaagc cctcagaatc 1141 cattctgtgg aaggtgacct caggagaaaa tcagctggac aggaggaatg gagccccagt 1201 gctccctcac ccccaggaag ctgtgtccag gcagaagctg cacctgctgg gctctgtgga 1261 gagcagcggg gagaggactg tgccgagctg catgactact tcaatgtcct gagttacaga 1321 agcctgggta actgcagctt cttcacagag actggtgaat tcgccaccat gTACCCATAC 1381 GACGTACCTG ATTATGCAAC TTATCCTTAT GATGTACCTG ATTATGCTAG CTATCCTTAT 1441 GATGTCCCAG ACTACGCAgg ggatccaccg gtcgccacca tggtgagcaa gggcgaggag 1501 gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg 1561 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag 1621 accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc 1681 cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac 1741 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc 1801 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg 1861 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc 1921 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag 1981 cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag 2041 gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc 2101 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc 2161 accggcggca tggacgagct gtacaagtag cggccgcgac tctagatcat aatcagccat 2221 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg 2281 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac 2341 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 2401 tgtggtttgt ccaaactcat caatgtatct taaggcgtaa attgtaagcg ttaatatttt 2461 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat 2521 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt 2581 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt 2641 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag 2701 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg 2761 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc 2821 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc 2881 gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt 2941 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct 3001 tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt 3061 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat 3121 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg 3181 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg 3241 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 3301 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt 3361 tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat 3421 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg 3481 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc 3541 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca 3601 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct 3661 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga 3721 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg 3781 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat 3841 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga 3901 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg 3961 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg 4021 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat 4081 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct 4141 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga 4201 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg 4261 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt 4321 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc 4381 caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct 4441 atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc 4501 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa 4561 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg 4621 ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt 4681 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 4741 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 4801 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 4861 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 4921 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag 4981 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 5041 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 5101 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 5161 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 5221 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 5281 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 5341 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 5401 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 5461 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg 5521 cat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.