pCMV-Kif20a-3×Myc-Neo plasmid

pCMV-Kif20a-3×Myc-Neo plasmid

PVT96607

pCMV-Kif20a-3×Myc-Neo plasmid Information

Plasmid name: pCMV-Kif20a(mouse)-3×Myc-Neo
Alias: NM_001166406.1;Rab6kifl
Gene lenght: 2661bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Kif20a-3×Myc-Neo plasmid Description

pCMV-Kif20a-3×Myc-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-Kif20a-3×Myc-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGTTTAAAC TTAAGCTTGG 661 TACCGAGCTC GGATCCACTA GTCCAGTGTG GTGGAATTCG CCACCatgtc tcaccggatc 721 ctttctccgc cagcgggctt actctctgat gaggatgttg tagactctcc catattagaa 781 tccacagctg cagatctgag atctgttgtc cgaaaggacc tgttgtcaga ctgctctgtc 841 atctctgcct ctctggagga caagcaggct ctactggagg acacctcaga gaaggtgaaa 901 gtttaccttc gaattcgacc attcttgact tcagagttgg accgacagga agatcagggc 961 tgcgtctgca ttgagaatac agaaaccctt gtgctgcagg cacccaaaga ctcctttgcc 1021 ttgaagagta atgagagggg agttggtcag gccactcaca aatttacctt ttcccagata 1081 tttgggccag aagtgggaca ggtggccttc ttcaatctga ccatgaagga gatggtgaag 1141 gatgtgctca aagggcagaa ctggctcatc tacacctatg gagtcaccaa ctcggggaaa 1201 acgtacacca ttcaaggtac tagtaaggat gcggggatcc tgccccagtc cctggctctc 1261 atctttaata gtctccaggg ccagcttcat ccgacacctg acctgaagcc cttattgtcc 1321 aatgaagtaa tctggctaga cagcaagcag attcgacagg aggaaatgaa gaagctgtcc 1381 ttgctaattg gaggcctcca agaggaggag ctgtccacct ccgtgaagaa acgtgtccac 1441 actgaaagtc ggataggtgc cagcaacagc tttgacagtg gcgttgctgg gctgtcttcc 1501 accagtcagt tcaccagcag cagccagcta gatgaaacaa gtcaattatg ggcacaacca 1561 gacactgtcc cagtaagtgt tccagcagac attcgcttct ctgtctggat ctccttcttt 1621 gagatctaca atgaattgct ttatgacctg ttagaaccac ctagccatca gcataagaga 1681 cagacacttc ggctgtgtga ggatcagaac ggcaatcctt acgtgaaaga tctcaattgg 1741 attcatgttc gtgatgttga ggaagcctgg aaactattga aagtgggtcg caagaaccag 1801 agctttgcca gcacccacat gaaccagcaa tccagccgca gtcacagcat cttctcaatc 1861 agaatcttgc accttcaggg agaaggggat atagtgccca agatcagtga actgtcactc 1921 tgtgacctgg ctggctctga gcgctgcaaa catcaaaaaa gtggtgagcg gctaaaggag 1981 gcagggaaca ttaacacttc tctgcacacc ctgggccgct gtattgctgc cctgcgacag 2041 aatcagcaga accggtcaaa gcagaacctg attcctttcc gtgacagcaa gttgactcgt 2101 gtgttccaag gcttcttcac aggtcgaggt cgttcctgta tgattgtcaa tgtgaatccc 2161 tgtgcatcta catatgacga gactcttcat gcagccaaat tctcagccct agccagccag 2221 cttgtgcacg ccccacctgt gcatctggga atcccatccc tgcattcatt catcaagaag 2281 cacagtcctc aggttggccc tggcttagag aaagaggaca aggccgactc agaccttgag 2341 gacagtcctg aagacgaagc tgacgtctcc gtgtatggca aggaggaact actccaggtg 2401 gtggaagcca tgaaagcgct acttttaaaa gaacgacaag aaaagctgca gttggagata 2461 cagctccgag aggaaatttg caacgaaatg gtggaacaga tgcaacagcg ggagcagtgg 2521 tgcagtgagc gtttggacaa ccaaaaggaa ctgatggagg aactgtacga ggagaaactg 2581 aagatcctga aggaatcact gacaactttc taccaagagc agatccagga gcgggatgaa 2641 aaaattgaag agctagaaac tctgttgcag gaagccaaac agcagccagc ggcacaacag 2701 tcaggggggc tgtccctcct ccggcggtca cagaggctgg cagcgtctgc ctccactcag 2761 cagttccagg aagttaaagc tgaactagag caatgcaaaa cagagttaag ctctaccact 2821 gcagagctgc acaagtatca gcaagtgtta aaaccgccac ccccagccaa gcctttcacc 2881 attgatgtgg ataagaagtt agaggagggc cagaagaata taagactgct acggacagag 2941 ctccagaaac taggacagtc tctgcagtca gcagaaagag cctgttgcca cagcaccgga 3001 gcaggaaaac ttcgtcaagc attaaccaac tgtgatgaca tcttaatcaa gcagaaccag 3061 accctggctg agctgcagaa taacatggtg ctagtgaaac tggaccttca gaaaaaggca 3121 gcctgcattg ctgagcagta tcacactgtg ctaaagctcc aaggccaagc ctctgccaaa 3181 aagcggctgg gagcaaacca ggaaaaccag caaccaaatc atcaaccccc aggaaagaaa 3241 ccattcctgc gaaacttact tccccgaaca cctacctgcc aaagctcaac agacagcagc 3301 ccttatgcgc ggatcttgcg ctcacggcac tctcctttac tcaaatctcc ctttggcaaa 3361 aaatacCTCG AGGAACAAAA ACTAATATCA GAGGAAGATT TGGAACAGAA ACTAATCAGT 3421 GAAGAAGATT TAGAGCAAAA GTTAATTTCC GAGGAGGACT TATAGTGAGG GCCCGATATC 3481 TCGACAATCA ACCTCTGGAT TACAAAATTT GTGAAAGATT GACTGGTATT CTTAACTATG 3541 TTGCTCCTTT TACGCTATGT GGATACGCTG CTTTAATGCC TTTGTATCAT GCTATTGCTT 3601 CCCGTATGGC TTTCATTTTC TCCTCCTTGT ATAAATCCTG GTTGCTGTCT CTTTATGAGG 3661 AGTTGTGGCC CGTTGTCAGG CAACGTGGCG TGGTGTGCAC TGTGTTTGCT GACGCAACCC 3721 CCACTGGTTG GGGCATTGCC ACCACCTGTC AGCTCCTTTC CGGGACTTTC GCTTTCCCCC 3781 TCCCTATTGC CACGGCGGAA CTCATCGCCG CCTGCCTTGC CCGCTGCTGG ACAGGGGCTC 3841 GGCTGTTGGG CACTGACAAT TCCGTGGTGT TGTCGGGGAA GCTGACGTCC TTTCCATGGC 3901 TGCTCGCCTG TGTTGCCACC TGGATTCTGC GCGGGACGTC CTTCTGCTAC GTCCCTTCGG 3961 CCCTCAATCC AGCGGACCTT CCTTCCCGCG GCCTGCTGCC GGCTCTGCGG CCTCTTCCGC 4021 GTCTTCGCCT TCGCCCTCAG ACGAGTCGGA TCTCCCTTTG GGCCGCCTCC CCGCCTGGAA 4081 ACGGGGGAGG CTAACTGAAA CACGGAAGGA GACAATACCG GAAGGAACCC GCGCTATGAC 4141 GGCAATAAAA AGACAGAATA AAACGCACGG GTGTTGGGTC GTTTGTTCAT AAACGCGGGG 4201 TTCGGTCCCA GGGCTGGCAC TCTGTCGATA CCCCACCGAG ACCCCATTGG GGCCAATACG 4261 CCCGCGTTTC TTCCTTTTCC CCACCCCACC CCCCAAGTTC GGGTGAAGGC CCAGGGCTCG 4321 CAGCCAACGT CGGGGCGGCA GGCCCTGCCA TAGCAGATCT GCGCAGCTGG GGCTCTAGGG 4381 GGTATCCCCA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA 4441 GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT 4501 TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGCATC CCTTTAGGGT 4561 TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC 4621 GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT 4681 TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT 4741 TTGATTTATA AGGGATTTTG GGGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC 4801 AAAAATTTAA CGCGAATTAA TTCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC 4861 AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG 4921 TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC 4981 AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC 5041 CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC 5101 TGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA 5161 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC 5221 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG 5281 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG 5341 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA 5401 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC 5461 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC 5521 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA 5581 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA 5641 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT 5701 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT 5761 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT 5821 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA 5881 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA 5941 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG 6001 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG CGAAATGACC GACCAAGCGA 6061 CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT 6121 TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG 6181 AGTTCTTCGC CCACCCCAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA 6241 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA 6301 AACTCATCAA TGTATCTTAT CATGTCTGTA TACCGTCGAC CTCTAGCTAG AGCTTGGCGT 6361 AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA 6421 TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT 6481 TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT 6541 AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT 6601 CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA 6661 AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA 6721 AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC 6781 TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA 6841 CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC 6901 CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT 6961 CTCAATGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT 7021 GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG 7081 AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA 7141 GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT 7201 ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA 7261 GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT 7321 GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA 7381 CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT 7441 CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA 7501 GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT 7561 CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA 7621 CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT 7681 CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG 7741 GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA 7801 GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT 7861 CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA 7921 CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA 7981 GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA 8041 CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT 8101 GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG 8161 CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC 8221 TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT 8281 GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA 8341 ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT 8401 TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT 8461 GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG 8521 ACGTCGACGG ATCGGGAGAT CTCCCGATCC CCTATGGTCG ACTCTCAGTA CAATCTGCTC 8581 TGATGCCGCA TAGTTAAGCC AGTATCTGCT CCCTGCTTGT GTGTTGGAGG TCGCTGAGTA 8641 GTGCGCGAGC AAAATTTAAG CTACAACAAG GCAAGGCTTG ACCGACAATT GCATGAAGAA 8701 TCTGCTTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.