phSyn-MCS-P2A-mCherry-Neo plas

phSyn-MCS-P2A-mCherry-Neo plasmid

PVT96602

phSyn-MCS-P2A-mCherry-Neo plasmid Information

Plasmid name: phSyn-MCS-P2A-mCherry-Neo
Alias: -
Gene lenght:
Host: Mammalian cells
Use(s):
Promoter detection
Fragment type: Promoter
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Red
Promoter: hSyn
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: phSyn-F：cttgtgtgttggaggtcgctgag
Reverse primer:
Copy number: High
Induction:

phSyn-MCS-P2A-mCherry-Neo plasmid Description

phSyn-MCS-P2A-mCherry-Neo is a plasmid suitable for promoter detection in mammalian cells.

phSyn-MCS-P2A-mCherry-Neo plasmid Sequence

1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttc tgcagagggc 61 cctgcgtatg agtgcaagtg ggttttagga ccaggatgag gcggggtggg ggtgcctacc 121 tgacgaccga ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc 181 ccctatcaga gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt 241 cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca 301 ctgaaggcgc gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg 361 gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg 421 ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg 481 tgggcagcgg aggagtcgtg tcgtgcctga gagcgcagcg tttagtgaac cgtcagatcg 541 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc 601 tccggactct agagctagcg tttaaactta agcttggtac cgagctcgga tccactagtc 661 cagtgtggtg gaattcggaa gcggagctac taacttcagc ctgctgaagc aggctggcga 721 cgtggaggag aaccctggac ctatggtgag caagggcgag gaggataaca tggccatcat 781 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga 841 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt 901 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg 961 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc 1021 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac 1081 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa 1141 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga 1201 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa 1261 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca 1321 gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta 1381 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga 1441 gctgtacaag taactcgagt ctagagggcc ccttaagggt tcgatcccta ccggttagta 1501 atgagtttga tatctcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg 1561 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta 1621 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct 1681 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt 1741 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac 1801 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg 1861 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac 1921 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg 1981 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct 2041 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc 2101 ctccccgcct ggaaacgggg gaggctaact gaaacacgga aggagacaat accggaagga 2161 acccgcgcta tgacggcaat aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt 2221 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca 2281 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga 2341 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcag atctgcgcag 2401 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt 2461 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 2521 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg 2581 catcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta 2641 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt 2701 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 2761 ctcggtctat tcttttgatt tataagggat tttggggatt tcggcctatt ggttaaaaaa 2821 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg 2881 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 2941 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg 3001 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact 3061 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag 3121 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc 3181 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag 3241 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc 3301 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat 3361 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg 3421 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg 3481 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta 3541 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta 3601 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc 3661 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc 3721 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg 3781 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg 3841 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt 3901 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc 3961 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc 4021 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgcgaaat 4081 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta 4141 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg 4201 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta 4261 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 4321 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag 4381 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 4441 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 4501 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 4561 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 4621 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 4681 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 4741 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 4801 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 4861 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 4921 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 4981 aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg 5041 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 5101 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 5161 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 5221 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 5281 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 5341 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 5401 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 5461 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 5521 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 5581 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 5641 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 5701 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 5761 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 5821 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 5881 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 5941 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 6001 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 6061 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 6121 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 6181 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 6241 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 6301 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 6361 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 6421 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 6481 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 6541 aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg gtcgactctc 6601 agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg 6661 gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac 6721 aattgcatga agaatctgct tagg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.