pSIN-EF2-FLAG-DGAT2-Puro plasm

pSIN-EF2-FLAG-DGAT2-Puro plasmid

PVT96459

pSIN-EF2-FLAG-DGAT2-Puro plasmid Information

Plasmid name: pSIN-EF2-FLAG-DGAT2(human)-Puro
Alias: NM_001253891.2;ARAT; GS1999FULL; HMFN1045
Gene lenght: 1038bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: EF1a
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: EF1a-R-F
Reverse primer:
Copy number: High
Induction:

pSIN-EF2-FLAG-DGAT2-Puro plasmid Description

pSIN-EF2-FLAG-DGAT2-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pSIN-EF2-FLAG-DGAT2-Puro plasmid Sequence

1 CGGACCGCCA CTGCCAATTA CCTGTGGTTT CATTTACTCT AAACCTGTGA TTCCTCTGAA 61 TTATTTTCAT TTTAAAGAAA TTGTATTTGT TAAATATGTA CTACAAACTT AGTAGTTGGA 121 AGGGCTAATT CACTCCCAAA GAAGACAAGA TATCCTTGAT CTGTGGATCT ACCACACACA 181 AGGCTACTTC CCTGATTAGC AGAACTACAC ACCAGGGCCA GGGGTCAGAT ATCCACTGAC 241 CTTTGGATGG TGCTACAAGC TAGTACCAGT TGAGCCAGAT AAGGTAGAAG AGGCCAATAA 301 AGGAGAGAAC ACCAGCTTGT TACACCCTGT GAGCCTGCAT GGGATGGATG ACCCGGAGAG 361 AGAAGTGTTA GAGTGGAGGT TTGACAGCCG CCTAGCATTT CATCACGTGG CCCGAGAGCT 421 GCATCCGGAG TACTTCAAGA ACTGCTGATA TCGAGCTTGC TACAAGGGAC TTTCCGCTGG 481 GGACTTTCCA GGGAGGCGTG GCCTGGGCGG GACTGGGGAG TGGCGAGCCC TCAGATCCTG 541 CATATAAGCA GCTGCTTTTT GCCTGTACTG GGTCTCTCTG GTTAGACCAG ATCTGAGCCT 601 GGGAGCTCTC TGGCTAACTA GGGAACCCAC TGCTTAAGCC TCAATAAAGC TTGCCTTGAG 661 TGCTTCAAGT AGTGTGTGCC CGTCTGTTGT GTGACTCTGG TAACTAGAGA TCCCTCAGAC 721 CCTTTTAGTC AGTGTGGAAA ATCTCTAGCA GTGGCGCCCG AACAGGGACT TGAAAGCGAA 781 AGGGAAACCA GAGGAGCTCT CTCGACGCAG GACTCGGCTT GCTGAAGCGC GCACGGCAAG 841 AGGCGAGGGG CGGCGACTGG TGAGTACGCC AAAAATTTTG ACTAGCGGAG GCTAGAAGGA 901 GAGAGATGGG TGCGAGAGCG TCAGTATTAA GCGGGGGAGA ATTAGATCGC GATGGGAAAA 961 AATTCGGTTA AGGCCAGGGG GAAAGAAAAA ATATAAATTA AAACATATAG TATGGGCAAG 1021 CAGGGAGCTA GAACGATTCG CAGTTAATCC TGGCCTGTTA GAAACATCAG AAGGCTGTAG 1081 ACAAATACTG GGACAGCTAC AACCATCCCT TCAGACAGGA TCAGAAGAAC TTAGATCATT 1141 ATATAATACA GTAGCAACCC TCTATTGTGT GCATCAAAGG ATAGAGATAA AAGACACCAA 1201 GGAAGCTTTA GACAAGATAG AGGAAGAGCA AAACAAAAGT AAGACCACCG CACAGCAAGC 1261 GGCCGCTGAT CTTCAGACCT GGAGGAGGAG ATATGAGGGA CAATTGGAGA AGTGAATTAT 1321 ATAAATATAA AGTAGTAAAA ATTGAACCAT TAGGAGTAGC ACCCACCAAG GCAAAGAGAA 1381 GAGTGGTGCA GAGAGAAAAA AGAGCAGTGG GAATAGGAGC TTTGTTCCTT GGGTTCTTGG 1441 GAGCAGCAGG AAGCACTATG GGCGCAGCGT CAATGACGCT GACGGTACAG GCCAGACAAT 1501 TATTGTCTGG TATAGTGCAG CAGCAGAACA ATTTGCTGAG GGCTATTGAG GCGCAACAGC 1561 ATCTGTTGCA ACTCACAGTC TGGGGCATCA AGCAGCTCCA GGCAAGAATC CTGGCTGTGG 1621 AAAGATACCT AAAGGATCAA CAGCTCCTGG GGATTTGGGG TTGCTCTGGA AAACTCATTT 1681 GCACCACTGC TGTGCCTTGG AATGCTAGTT GGAGTAATAA ATCTCTGGAA CAGATTTGGA 1741 ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA TTACACAAGC TTAATACACT 1801 CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA ACAAGAATTA TTGGAATTAG 1861 ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA TTGGCTGTGG TATATAAAAT 1921 TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT AGTTTTTGCT GTACTTTCTA 1981 TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT TCAGACCCAC CTCCCAACCC 2041 CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG TGGAGAGAGA GACAGAGACA 2101 GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGCCA CAAATGGCAG TATTCATCCA 2161 CAATTTTAAA AGAAAGGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 2221 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAA TTCAAAATTT 2281 TCGGGTTTAT TACAGGGACA GCAGAGATCC ACTTTGGATC GATAAGCTTT GCAAAGATGG 2341 ATAAAGTTTT AAACAGAGAG GAATCTTTGC AGCTAATGGA CCTTCTAGGT CTTGAAAGGA 2401 GTGGGAATTG GCTCCGGTGC CCGTCAGTGG GCAGAGCGCA CATCGCCCAC AGTCCCCGAG 2461 AAGTTGGGGG GAGGGGTCGG CAATTGAACC GGTGCCTAGA GAAGGTGGCG CGGGGTAAAC 2521 TGGGAAAGTG ATGTCGTGTA CTGGCTCCGC CTTTTTCCCG AGGGTGGGGG AGAACCGTAT 2581 ATAAGTGCAG TAGTCGCCGT GAACGTTCTT TTTCGCAACG GGTTTGCCGC CAGAACACAG 2641 GTAAGTGCCG TGTGTGGTTC CCGCGGGCCT GGCCTCTTTA CGGGTTATGG CCCTTGCGTG 2701 CCTTGAATTA CTTCCACCTG GCTGCAGTAC GTGATTCTTG ATCCCGAGCT TCGGGTTGGA 2761 AGTGGGTGGG AGAGTTCGAG GCCTTGCGCT TAAGGAGCCC CTTCGCCTCG TGCTTGAGTT 2821 GAGGCCTGGC CTGGGCGCTG GGGCCGCCGC GTGCGAATCT GGTGGCACCT TCGCGCCTGT 2881 CTCGCTGCTT TCGATAAGTC TCTAGCCATT TAAAATTTTT GATGACCTGC TGCGACGCTT 2941 TTTTTCTGGC AAGATAGTCT TGTAAATGCG GGCCAAGATC TGCACACTGG TATTTCGGTT 3001 TTTGGGGCCG CGGGCGGCGA CGGGGCCCGT GCGTCCCAGC GCACATGTTC GGCGAGGCGG 3061 GGCCTGCGAG CGCGGCCACC GAGAATCGGA CGGGGGTAGT CTCAAGCTGG CCGGCCTGCT 3121 CTGGTGCCTG GCCTCGCGCC GCCGTGTATC GCCCCGCCCT GGGCGGCAAG GCTGGCCCGG 3181 TCGGCACCAG TTGCGTGAGC GGAAAGATGG CCGCTTCCCG GCCCTGCTGC AGGGAGCTCA 3241 AAATGGAGGA CGCGGCGCTC GGGAGAGCGG GCGGGTGAGT CACCCACACA AAGGAAAAGG 3301 GCCTTTCCGT CCTCAGCCGT CGCTTCATGT GACTCCACGG AGTACCGGGC GCCGTCCAGG 3361 CACCTCGATT AGTTCTCGAG CTTTTGGAGT ACGTCGTCTT TAGGTTGGGG GGAGGGGTTT 3421 TATGCGATGG AGTTTCCCCA CACTGAGTGG GTGGAGACTG AAGTTAGGCC AGCTTGGCAC 3481 TTGATGTAAT TCTCCTTGGA ATTTGCCCTT TTTGAGTTTG GATCTTGGTT CATTCTCAAG 3541 CCTCAGACAG TGGTTCAAAG TTTTTTTCTT CCATTTCAGG TGTCGTGAGG AATTGGGATC 3601 CCCGGACGAA TTCgccacca tgGATTACAA GGACGACGAT GACAAGCATA TGatgaagac 3661 cctcatagcc gcctactccg gggtcctgcg cggcgagcgt caggccgagg ctgaccggag 3721 ccagcgctct cacggaggac ctgcgctgtc gcgcgagggg tctgggagat ggggagtggc 3781 ctgcagtgcc atcctcatgt acatattctg cactgattgc tggctcatcg ctgtgctcta 3841 cttcacttgg ctggtgtttg actggaacac acccaagaaa ggtggcagga ggtcacagtg 3901 ggtccgaaac tgggctgtgt ggcgctactt tcgagactac tttcccatcc agctggtgaa 3961 gacacacaac ctgctgacca ccaggaacta tatctttgga taccaccccc atggtatcat 4021 gggcctgggt gccttctgca acttcagcac agaggccaca gaagtgagca agaagttccc 4081 aggcatacgg ccttacctgg ctacactggc aggcaacttc cgaatgcctg tgttgaggga 4141 gtacctgatg tctggaggta tctgccctgt cagccgggac accatagact atttgctttc 4201 aaagaatggg agtggcaatg ctatcatcat cgtggtcggg ggtgcggctg agtctctgag 4261 ctccatgcct ggcaagaatg cagtcaccct gcggaaccgc aagggctttg tgaaactggc 4321 cctgcgtcat ggagctgacc tggttcccat ctactccttt ggagagaatg aagtgtacaa 4381 gcaggtgatc ttcgaggagg gctcctgggg ccgatgggtc cagaagaagt tccagaaata 4441 cattggtttc gccccatgca tcttccatgg tcgaggcctc ttctcctccg acacctgggg 4501 gctggtgccc tactccaagc ccatcaccac tgttgtggga gagcccatca ccatccccaa 4561 gctggagcac ccaacccagc aagacatcga cctgtaccac accatgtaca tggaggccct 4621 ggtgaagctc ttcgacaagc acaagaccaa gttcggcctc ccggagactg aggtcctgga 4681 ggtgaactga ACTAGTGATC CGCATGCATC TAGGGCGGCC AATTCCGCCC CTCTCCCCCC 4741 CCCCCCTCTC CCTCCCCCCC CCCTAACGTT ACTGGCCGAA GCCGCTTGGA ATAAGGCCGG 4801 TGTGCGTTTG TCTATATGTT ATTTTCCACC ATATTGCCGT CTTTTGGCAA TGTGAGGGCC 4861 CGGAAACCTG GCCCTGTCTT CTTGACGAGC ATTCCTAGGG GTCTTTCCCC TCTCGCCAAA 4921 GGAATGCAAG GTCTGTTGAA TGTCGTGAAG GAAGCAGTTC CTCTGGAAGC TTCTTGAAGA 4981 CAAACAACGT CTGTAGCGAC CCTTTGCAGG CAGCGGAACC CCCCACCTGG CGACAGGTGC 5041 CTCTGCGGCC AAAAGCCACG TGTATAAGAT ACACCTGCAA AGGCGGCACA ACCCCAGTGC 5101 CACGTTGTGA GTTGGATAGT TGTGGAAAGA GTCAAATGGC TCTCCTCAAG CGTATTCAAC 5161 AAGGGGCTGA AGGATGCCCA GAAGGTACCC CATTGTATGG GATCTGATCT GGGGCCTCGG 5221 TGCACATGCT TTACATGTGT TTAGTCGAGG TTAAAAAAAC GTCTAGGCCC CCCGAACCAC 5281 GGGGACGTGG TTTTCCTTTG AAAAACACGA TGATAAGCTT GCCACAACCC ACAAGGAGAC 5341 GACCTTCCAT GACCGAGTAC AAGCCCACGG TGCGCCTCGC CACCCGCGAC GACGTCCCCC 5401 GGGCCGTACG CACCCTCGCC GCCGCGTTCG CCGACTACCC CGCCACGCGC CACACCGTCG 5461 ACCCGGACCG CCACATCGAG CGGGTCACCG AGCTGCAAGA ACTCTTCCTC ACGCGCGTCG 5521 GGCTCGACAT CGGCAAGGTG TGGGTCGCGG ACGACGGCGC CGCGGTGGCG GTCTGGACCA 5581 CGCCGGAGAG CGTCGAAGCG GGGGCGGTGT TCGCCGAGAT CGGCCCGCGC ATGGCCGAGT 5641 TGAGCGGTTC CCGGCTGGCC GCGCAGCAAC AGATGGAAGG CCTCCTGGCG CCGCACCGGC 5701 CCAAGGAGCC CGCGTGGTTC CTGGCCACCG TCGGCGTCTC GCCCGACCAC CAGGGCAAGG 5761 GTCTGGGCAG CGCCGTCGTG CTCCCCGGAG TGGAGGCGGC CGAGCGCGCC GGGGTGCCCG 5821 CCTTCCTGGA GACCTCCGCG CCCCGCAACC TCCCCTTCTA CGAGCGGCTC GGCTTCACCG 5881 TCACCGCCGA CGTCGAGGTG CCCGAAGGAC CGCGCACCTG GTGCATGACC CGCAAGCCCG 5941 GTGCCTGATA ATAGGCGGCC GCTCGAGACC TAGAAAAACA TGGAGCAATC ACAAGTAGCA 6001 ATACAGCAGC TACCAATGCT GATTGTGCCT GGCTAGAAGC ACAAGAGGAG GAGGAGGTGG 6061 GTTTTCCAGT CACACCTCAG GTACCTTTAA GACCAATGAC TTACAAGGCA GCTGTAGATC 6121 TTAGCCACTT TTTAAAAGAA AAGGGGGGAC TGGAAGGGCT AATTCACTCC CAACGAAGAC 6181 AAGATCTGCT TTTTGCTTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC 6241 TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC 6301 AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT 6361 AGTCAGTGTG GAAAATCTCT AGCAGTAGTA GTTCATGTCA TCTTATTATT CAGTATTTAT 6421 AACTTGCAAA GAAATGAATA TCAGAGAGTG AGAGGCCTTG ACATTATAAT AGATTTAGCA 6481 GGAATTGAAC TAGGAGTGGA GCACACAGGC AAAGTTCTAG AGCTCGCTGA TCAGCCTCGA 6541 CTGTGCCTTC TAGTTGCCAG CCATCTGTTG TTTGCCCCTC CCCCGTGCCT TCCTTGACCC 6601 TGGAAGGTGC CACTCCCACT GTCCTTTCCT AATAAAATGA GGAAATTGCA TCGCATTGTC 6661 TGAGTAGGTG TCATTCTATT CTGGGGGGTG GGGGTGGGGC AGGACAGCAA GGGGGGAGGA 6721 TTGGGAAGAC AATAGCAGGC ATGCTGGGGA TGCGGTGGGC TCTATGGCTT CTGAGGCGGA 6781 AAGAACCAGC TGGGGGCGCG CCCCTCGAGG CCGCCATGGT CATAGCTGTT TGACGTCAGG 6841 TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC 6901 AAATATGTAT CCGCTCACGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG 6961 GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG 7021 CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT 7081 GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT 7141 TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT 7201 ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA 7261 TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG 7321 AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC 7381 AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC 7441 TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC 7501 CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC 7561 TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT 7621 TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG 7681 TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT 7741 TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT 7801 AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA 7861 GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA 7921 TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA 7981 AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC 8041 AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT 8101 TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTTCTTC TAGTGTAGCC 8161 GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT 8221 CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG 8281 ACGATAGTTA CCGGATAAGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC 8341 AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC 8401 GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA 8461 GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG 8521 TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA 8581 TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCTGGC CTTTTGCA

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.