pLV3-CMV-INSR-EGFP-EF1a-Puro p

pLV3-CMV-INSR-EGFP-EF1a-Puro plasmid

PVT92742

pLV3-CMV-INSR-EGFP-EF1a-Puro plasmid Information

Plasmid name: pLV3-CMV-INSR(human)-EGFP-EF1a-Puro
Alias: NM_000208.4;CD220; HHF5
Gene lenght: 4146bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-INSR-EGFP-EF1a-Puro plasmid Description

pLV3-CMV-INSR-EGFP-EF1a-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-INSR-EGFP-EF1a-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc 2281 tagcgaattc GCCACCatgg ccaccggggg ccggcggggg gcggcggccg cgccgctgct 2341 ggtggcggtg gccgcgctgc tactgggcgc cgcgggccac ctgtaccccg gagaggtgtg 2401 tcccggcatg gatatccgga acaacctcac taggttgcat gagctggaga attgctctgt 2461 catcgaagga cacttgcaga tactcttgat gttcaaaacg aggcccgaag atttccgaga 2521 cctcagtttc cccaaactca tcatgatcac tgattacttg ctgctcttcc gggtctatgg 2581 gctcgagagc ctgaaggacc tgttccccaa cctcacggtc atccggggat cacgactgtt 2641 ctttaactac gcgctggtca tcttcgagat ggttcacctc aaggaactcg gcctctacaa 2701 cctgatgaac atcacccggg gttctgtccg catcgagaag aacaatgagc tctgttactt 2761 ggccactatc gactggtccc gtatcctgga ttccgtggag gataattaca tcgtgttgaa 2821 caaagatgac aacgaggagt gtggagacat ctgtccgggt accgcgaagg gcaagaccaa 2881 ctgccccgcc accgtcatca acgggcagtt tgtcgaacga tgttggactc atagtcactg 2941 ccagaaagtt tgcccgacca tctgtaagtc acacggctgc accgccgaag gcctctgttg 3001 ccacagcgag tgcctgggca actgttctca gcccgacgac cccaccaagt gcgtggcctg 3061 ccgcaacttc tacctggacg gcaggtgtgt ggagacctgc ccgcccccgt actaccactt 3121 ccaggactgg cgctgtgtga acttcagctt ctgccaggac ctgcaccaca aatgcaagaa 3181 ctcgcggagg cagggctgcc accagtacgt cattcacaac aacaagtgca tccctgagtg 3241 tccctccggg tacacgatga attccagcaa cttgctgtgc accccatgcc tgggtccctg 3301 tcccaaggtg tgccacctcc tagaaggcga gaagaccatc gactcggtga cgtctgccca 3361 ggagctccga ggatgcaccg tcatcaacgg gagtctgatc atcaacattc gaggaggcaa 3421 caatctggca gctgagctag aagccaacct cggcctcatt gaagaaattt cagggtatct 3481 aaaaatccgc cgatcctacg ctctggtgtc actttccttc ttccggaagt tacgtctgat 3541 tcgaggagag accttggaaa ttgggaacta ctccttctat gccttggaca accagaacct 3601 aaggcagctc tgggactgga gcaaacacaa cctcaccatc actcagggga aactcttctt 3661 ccactataac cccaaactct gcttgtcaga aatccacaag atggaagaag tttcaggaac 3721 caaggggcgc caggagagaa acgacattgc cctgaagacc aatggggacc aggcatcctg 3781 tgaaaatgag ttacttaaat tttcttacat tcggacatct tttgacaaga tcttgctgag 3841 atgggagccg tactggcccc ccgacttccg agacctcttg gggttcatgc tgttctacaa 3901 agaggcccct tatcagaatg tgacggagtt cgacgggcag gatgcgtgtg gttccaacag 3961 ttggacggtg gtagacattg acccacccct gaggtccaac gaccccaaat cacagaacca 4021 cccagggtgg ctgatgcggg gtctcaagcc ctggacccag tatgccatct ttgtgaagac 4081 cctggtcacc ttttcggatg aacgccggac ctatggggcc aagagtgaca tcatttatgt 4141 ccagacagat gccaccaacc cctctgtgcc cctggatcca atctcagtgt ctaactcatc 4201 atcccagatt attctgaagt ggaaaccacc ctccgacccc aatggcaaca tcacccacta 4261 cctggttttc tgggagaggc aggcggaaga cagtgagctg ttcgagctgg attattgcct 4321 caaagggctg aagctgccct cgaggacctg gtctccacca ttcgagtctg aagattctca 4381 gaagcacaac cagagtgagt atgaggattc ggccggcgaa tgctgctcct gtccaaagac 4441 agactctcag atcctgaagg agctggagga gtcctcgttt aggaagacgt ttgaggatta 4501 cctgcacaac gtggttttcg tccccagaaa aacctcttca ggcactggtg ccgaggaccc 4561 taggccatct cggaaacgca ggtcccttgg cgatgttggg aatgtgacgg tggccgtgcc 4621 cacggtggca gctttcccca acacttcctc gaccagcgtg cccacgagtc cggaggagca 4681 caggcctttt gagaaggtgg tgaacaagga gtcgctggtc atctccggct tgcgacactt 4741 cacgggctat cgcatcgagc tgcaggcttg caaccaggac acccctgagg aacggtgcag 4801 tgtggcagcc tacgtcagtg cgaggaccat gcctgaagcc aaggctgatg acattgttgg 4861 ccctgtgacg catgaaatct ttgagaacaa cgtcgtccac ttgatgtggc aggagccgaa 4921 ggagcccaat ggtctgatcg tgctgtatga agtgagttat cggcgatatg gtgatgagga 4981 gctgcatctc tgcgtctccc gcaagcactt cgctctggaa cggggctgca ggctgcgtgg 5041 gctgtcaccg gggaactaca gcgtgcgaat ccgggccacc tcccttgcgg gcaacggctc 5101 ttggacggaa cccacctatt tctacgtgac agactattta gacgtcccgt caaatattgc 5161 aaaaattatc atcggccccc tcatctttgt ctttctcttc agtgttgtga ttggaagtat 5221 ttatctattc ctgagaaaga ggcagccaga tgggccgctg ggaccgcttt acgcttcttc 5281 aaaccctgag tatctcagtg ccagtgatgt gtttccatgc tctgtgtacg tgccggacga 5341 gtgggaggtg tctcgagaga agatcaccct ccttcgagag ctggggcagg gctccttcgg 5401 catggtgtat gagggcaatg ccagggacat catcaagggt gaggcagaga cccgcgtggc 5461 ggtgaagacg gtcaacgagt cagccagtct ccgagagcgg attgagttcc tcaatgaggc 5521 ctcggtcatg aagggcttca cctgccatca cgtggtgcgc ctcctgggag tggtgtccaa 5581 gggccagccc acgctggtgg tgatggagct gatggctcac ggagacctga agagctacct 5641 ccgttctctg cggccagagg ctgagaataa tcctggccgc cctcccccta cccttcaaga 5701 gatgattcag atggcggcag agattgctga cgggatggcc tacctgaacg ccaagaagtt 5761 tgtgcatcgg gacctggcag cgagaaactg catggtcgcc catgatttta ctgtcaaaat 5821 tggagacttt ggaatgacca gagacatcta tgaaacggat tactaccgga aagggggcaa 5881 gggtctgctc cctgtacggt ggatggcacc ggagtccctg aaggatgggg tcttcaccac 5941 ttcttctgac atgtggtcct ttggcgtggt cctttgggaa atcaccagct tggcagaaca 6001 gccttaccaa ggcctgtcta atgaacaggt gttgaaattt gtcatggatg gagggtatct 6061 ggatcaaccc gacaactgtc cagagagagt cactgacctc atgcgcatgt gctggcaatt 6121 caaccccaag atgaggccaa ccttcctgga gattgtcaac ctgctcaagg acgacctgca 6181 ccccagcttt ccagaggtgt cgttcttcca cagcgaggag aacaaggctc ccgagagtga 6241 ggagctggag atggagtttg aggacatgga gaatgtgccc ctggaccgtt cctcgcactg 6301 tcagagggag gaggcggggg gccgggatgg agggtcctcg ctgggtttca agcggagcta 6361 cgaggaacac atcccttaca cacacatgaa cggaggcaag aaaaacgggc ggattctgac 6421 cttgcctcgg tccaatcctt ccggatccGG AGGAGGGGGA AGCatggtga gcaagggcga 6481 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca 6541 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa 6601 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac 6661 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa 6721 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa 6781 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct 6841 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta 6901 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt 6961 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa 7021 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc 7081 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac 7141 cgccgccggg atcactctcg gcatggacga gctgtacaag taagcggccg cgaaggatct 7201 gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt 7261 tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg gtaaactggg 7321 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa 7381 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagctga 7441 agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga ggccgccatc 7501 cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg 7561 ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga 7621 gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct caactctacg 7681 tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc 7741 tagatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc 7801 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgacccg 7861 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc 7921 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg 7981 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc 8041 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag 8101 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg 8161 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc 8221 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc 8281 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc 8341 tgagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac 8401 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt 8461 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat 8521 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca 8581 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc 8641 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg 8701 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct 8761 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct 8821 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt 8881 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct 8941 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga 9001 aaagggggga ctggaagggc taattcactc ccaacgaaaa taagatctgc tttttgcttg 9061 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa 9121 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct 9181 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc 9241 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat 9301 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag 9361 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 9421 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt 9481 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc 9541 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt 9601 tgcagagacg gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat 9661 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc 9721 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga 9781 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt 9841 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 9901 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 9961 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 10021 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 10081 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 10141 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 10201 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 10261 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 10321 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 10381 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 10441 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg 10501 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 10561 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 10621 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 10681 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 10741 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc 10801 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga 10861 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca 10921 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc 10981 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat 11041 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc 11101 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt 11161 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc 11221 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg 11281 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt 11341 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg 11401 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga 11461 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg 11521 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg 11581 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt 11641 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc 11701 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 11761 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat 11821 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac 11881 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc 11941 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat 12001 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga 12061 tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg 12121 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct 12181 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg 12241 gccagtgcca agctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.