pLV3-CMV-PRNP-3×FLAG-mCherry-N

pLV3-CMV-PRNP-3×FLAG-mCherry-Neo plasmid

PVT96341

pLV3-CMV-PRNP-3×FLAG-mCherry-Neo plasmid Information

Plasmid name: pLV3-CMV-PRNP(human-repeat-opt)-3×FLAG-mCherry-Neo
Alias: NM_000311.5;AltPrP; ASCR; CD230; CJD; GSS; KURU;p27-30; PRIP; PrP; PrP27-30; PrP33-35C; PrPc
Gene lenght: 759bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-PRNP-3×FLAG-mCherry-Neo plasmid Description

pLV3-CMV-PRNP-3×FLAG-mCherry-Neo It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-PRNP-3×FLAG-mCherry-Neo plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcgcca ccatggcgaa ccttggctgc tggatgctgg ttctctttgt 2341 ggccacatgg agtgacctgg gcctctgcaa gaagCGGCCG AAGCCTGGAG GCTGGAATAC 2401 AGGTGGCAGC AGATACCCGG GGCAGGGTAG TCCTGGCGGC AACAGATACC CACCCCAGGG 2461 CGGAGGGGGA TGGGGTCAAC CACATGGAGG CGGGTGGGGA CAGCCACATG GAGGTGGCTG 2521 GGGACAGCCT CACGGGGGCG GTTGGGGGCA GCCACACGGA GGAGGTTGGG GCCAGGGAGG 2581 Aggcacccac agtcagtgga acaagccgag taagccaaaa accaacatga agcacatggc 2641 tggtgctgca gcagctgggg cagtggtggg gggccttggc ggctacatgc tgggaagtgc 2701 catgagcagg cccatcatac atttcggcag tgactatgag gaccgttact atcgtgaaaa 2761 catgcaccgt taccccaacc aagtgtacta caggcccatg gatgagtaca gcaaccagaa 2821 caactttgtg cacgactgcg tcaatatcac aatcaagcag cacacggtca ccacaaccac 2881 caagggggag aacttcaccg agaccgacgt taagatgatg gagcgcgtgg ttgagcagat 2941 gtgtatcacc cagtacgaga gggaatctca ggcctattac cagagaggat cgagcatggt 3001 cctcttctcc tctccacctg tgatcctcct gatctctttc ctcatcttcc tgatagtggg 3061 aggatccgac tacaaagacc atgacggtga ttataaagat catgacatcg actacaagga 3121 tgacgatgac aagtagcggc cgcgaaggat ctgcgatcgc tccggtgccc gtcagtgggc 3181 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg 3241 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct 3301 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt 3361 tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc 3421 acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc 3481 tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc 3541 gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac 3601 gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg 3661 ttacagatcc aagctgtgac cggcgcctac gctagacgcc accatggtga gcaagggcga 3721 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc 3781 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac 3841 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct 3901 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga 3961 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga 4021 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa 4081 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat 4141 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat 4201 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta 4261 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat 4321 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca 4381 ctccaccggc ggcatggacg agctgtacaa gggatctcgc gagggcagag gaagtcttct 4441 aacatgcggt gacgtggagg agaatcccgg ccctgccacc atggctgaac aagatggatt 4501 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca 4561 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct 4621 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct 4681 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc 4741 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct 4801 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga 4861 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg 4921 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc 4981 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac 5041 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat 5101 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga 5161 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc 5221 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagtcga 5281 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc 5341 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg 5401 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt 5461 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac 5521 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc 5581 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct 5641 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct 5701 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct 5761 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct 5821 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ggtaccttta 5881 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga 5941 ctggaagggc taattcactc ccaacgaaga taagatctgc tttttgcttg tactgggtct 6001 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 6061 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 6121 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt 6181 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt 6241 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 6301 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 6361 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt 6421 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 6481 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt tgcagagacg 6541 gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa 6601 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga 6661 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 6721 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 6781 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 6841 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 6901 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 6961 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 7021 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 7081 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 7141 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 7201 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 7261 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 7321 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 7381 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 7441 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 7501 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 7561 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 7621 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 7681 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 7741 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 7801 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 7861 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 7921 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 7981 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 8041 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 8101 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 8161 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 8221 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 8281 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 8341 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 8401 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 8461 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 8521 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 8581 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 8641 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 8701 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc 8761 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca 8821 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc 8881 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag 8941 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga 9001 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat 9061 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat 9121 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca 9181 agctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.