pT7-6×His-ClyA-EGFP plasmid

pT7-6×His-ClyA-EGFP plasmid

PVT96332

pT7-6×His-ClyA-EGFP plasmid Information

Plasmid name: pT7-6×His-ClyA(ecoli-optimized-ecoli)-EGFP
Alias: -
Gene lenght: 909bp
Host: E.coli
Use(s): Protein Expression
Fragment type: ORF
Species: E.coli
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Green
Promoter: T7
Replicon: pBR322
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: T7ter
Reverse primer:
Copy number: High
Induction:

pT7-6×His-ClyA-EGFP plasmid Description

pT7-6×His-ClyA-EGFP is a plasmid suitable for protein expression in Escherichia coli.

pT7-6×His-ClyA-EGFP plasmid Sequence

1 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat 61 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 121 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 181 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 241 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 301 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 361 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 421 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 481 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 541 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 601 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 661 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 721 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact 781 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 841 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 901 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 961 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1021 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1081 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1141 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1201 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1261 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1321 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1381 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1441 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1681 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 1741 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 1801 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 1861 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 1921 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 1981 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2041 tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc 2101 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc 2161 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt 2221 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac 2281 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga 2341 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc 2401 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg 2461 tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca 2521 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac 2581 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg 2641 ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga 2701 acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga 2761 agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc 2821 gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg 2881 tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga 2941 tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg 3001 tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc 3061 cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg 3121 acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt 3181 ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt 3241 taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg 3301 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat 3361 aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc 3421 ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt 3481 gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat 3541 cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg 3601 tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg 3661 cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg 3721 tgcctaatga gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc 3781 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 3841 gcgtattggg cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc 3901 ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca 3961 ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat 4021 cgtcgtatcc cactaccgag atatccgcac caacgcgcag cccggactcg gtaatggcgc 4081 gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct 4141 cattcagcat ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt 4201 ccgctatcgg ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac 4261 gcgccgagac agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga 4321 ccagatgctc cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg 4381 gtgtctggtc agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag 4441 caatggcatc ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga 4501 gaagattgtg caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca 4561 ccacgctggc acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg 4621 cgtgcagggc cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt 4681 gttgtgccac gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc 4741 gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga 4801 caccggcata ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt 4861 gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt 4921 ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg 4981 ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac 5041 agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg 5101 aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca 5161 cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc 5221 ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag 5281 aaataatttt gtttaacttt aagaaggaga tataccatgg gcagcagcca tcatcatcat 5341 catcacagca gcggcctggt gccgcgcggc agccatATGG CTAGCATGAC TGGTGGACAG 5401 CAAATGGGTC GCGGATCCAT GACCGAAATC GTTGCTGACA AAACCGTTGA AGTTGTTAAA 5461 AACGCTATCG AAACCGCTGA CGGTGCTCTG GACCTGTACA ACAAATACCT GGACCAGGTT 5521 ATCCCGTGGC AGACCTTCGA CGAAACCATC AAAGAACTGT CTCGTTTCAA ACAGGAATAC 5581 TCTCAGGCTG CTTCTGTTCT GGTTGGTGAC ATCAAAACCC TGCTGATGGA CTCTCAGGAC 5641 AAATACTTCG AAGCTACCCA GACCGTTTAC GAATGGTGCG GTGTTGCTAC CCAGCTGCTG 5701 GCTGCTTACA TCCTGCTGTT CGACGAATAC AACGAAAAAA AAGCTTCTGC TCAGAAAGAC 5761 ATCCTGATCA AAGTTCTGGA CGACGGTATC ACCAAACTGA ACGAAGCTCA GAAGTCGCTG 5821 CTGGTCAGCT CTCAGTCTTT CAACAACGCT TCTGGTAAAC TGCTGGCTCT GGACTCTCAG 5881 CTGACCAACG ACTTCTCTGA AAAATCTTCT TACTTCCAGT CTCAGGTTGA CAAAATCCGT 5941 AAAGAAGCTT ACGCTGGTGC TGCTGCTGGT GTTGTTGCTG GTCCGTTCGG TCTGATCATC 6001 TCTTACTCTA TCGCTGCTGG TGTTGTTGAA GGTAAACTGA TCCCGGAACT GAAAAACAAA 6061 CTGAAATCTG TTCAGAACTT CTTCACCACC CTGTCTAACA CCGTTAAACA GGCTAACAAA 6121 GACATCGACG CTGCTAAACT GAAACTGACC ACCGAAATCG CTGCTATCGG TGAAATCAAA 6181 ACCGAAACCG AAACCACCCG TTTCTACGTT GACTACGACG ACCTGATGCT GTCTCTGCTG 6241 AAAGAAGCTG CTAAAAAAAT GATCAACACC TGCAACGAAT ACCAGAAACG TCACGGTAAA 6301 AAAACCCTGT TCGAAGTTCC GGAAGTTaaa cgcatttggt ttattccgcg cagcagctgg 6361 tatgaacgcg cgGGTGGAGG CGGATCAGGA GGCGGTGGAT CTatggtgag caagggcgag 6421 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac 6481 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag 6541 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc 6601 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag 6661 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac 6721 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg 6781 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac 6841 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc 6901 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac 6961 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc 7021 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc 7081 gccgccggga tcactctcgg catggacgag ctgtacaagt agggatccgg ctgctaacaa 7141 agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct 7201 tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata 7261 tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg 7321 acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt 7381 tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca 7441 tgagaa

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.