pLV2-CMV-CRY2PHR-mCherry-AFDN-

pLV2-CMV-CRY2PHR-mCherry-AFDN-Puro plasmid

PVT96270

pLV2-CMV-CRY2PHR-mCherry-AFDN-Puro plasmid Information

Plasmid name: pLV2-CMV-CRY2PHR-mCherry-AFDN(human)-Puro
Alias: NM_001207008.2;AF6; l-afadin; MLL-AF6; MLLT4
Gene lenght: 5229bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-CRY2PHR-mCherry-AFDN-Puro plasmid Description

pLV2-CMV-CRY2PHR-mCherry-AFDN-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-CMV-CRY2PHR-mCherry-AFDN-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcTA GTCCAGTGTG 2821 GTGctcgaga tgaagatgga caaaaagact atagtttggt ttagaagaga cctaaggatt 2881 gaggataatc ctgcattagc agcagctgct cacgaaggat ctgtttttcc tgtcttcatt 2941 tggtgtcctg aagaagaagg acagttttat cctggaagag cttcaagatg gtggatgaaa 3001 caatcacttg ctcacttatc tcaatccttg aaggctcttg gatctgacct cactttaatc 3061 aaaacccaca acacgatttc agcgatcttg gattgtatcc gcgttaccgg tgctacaaaa 3121 gtcgtcttta accacctcta tgatcctgtt tcgttagttc gggaccatac cgtaaaggag 3181 aagctggtgg aacgtgggat ctctgtgcaa agctacaatg gagatctatt gtatgaaccg 3241 tgggagatat actgcgaaaa gggcaaacct tttacgagtt tcaattctta ctggaagaaa 3301 tgcttagata tgtcgattga atccgttatg cttcctcctc cttggcggtt gatgccaata 3361 actgcagcgg ctgaagcgat ttgggcgtgt tcgattgaag aactagggct ggagaatgag 3421 gccgagaaac cgagcaatgc gttgttaact agagcttggt ctccaggatg gagcaatgct 3481 gataagttac taaatgagtt catcgagaag cagttgatag attatgcaaa gaacagcaag 3541 aaagttgttg ggaattctac ttcactactt tctccgtatc tccatttcgg ggaaataagc 3601 gtcagacacg ttttccagtg tgcccggatg aaacaaatta tatgggcaag agataagaac 3661 agtgaaggag aagaaagtgc agatcttttt cttaggggaa tcggtttaag agagtattct 3721 cggtatatat gtttcaactt cccgtttact cacgagcaat cgttgttgag tcatcttcgg 3781 tttttccctt gggatgctga tgttgataag ttcaaggcct ggagacaagg caggaccggt 3841 tatccgttgg tggatgccgg aatgagagag ctttgggcta ccggatggat gcataacaga 3901 ataagagtga ttgtttcaag ctttgctgtg aagtttcttc tccttccatg gaaatgggga 3961 atgaagtatt tctgggatac acttttggat gctgatttgg aatgtgacat ccttggctgg 4021 cagtatatct ctgggagtat ccccgatggc cacgagcttg atcgcttgga caatcccgcg 4081 ttacaaggcg ccaaatatga cccagaaggt gagtacataa ggcaatggct tcccgagctt 4141 gcgagattgc caactgaatg gatccatcat ccatgggacg ctcctttaac cgtactcaaa 4201 gcttctggtg tggaactcgg aacaaactat gcgaaaccca ttgtagacat cgacacagct 4261 cgtgagctac tagctaaagc tatttcaaga acccgtgaag cacagatcat gatcggagca 4321 gcagcccggg atccaccggt cgccaccatg gtgagcaagg gcgaggagga taacatggcc 4381 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag 4441 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg 4501 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg 4561 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc 4621 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc 4681 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc 4741 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc 4801 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag 4861 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc 4921 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag 4981 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg 5041 gacgagctgt acaagAActc gagATGCAGA TGTAGACCaC TAGTaGGAGG AGGAAGCGGA 5101 GGAGGAAGCt cggcgggcgg ccgtgacgag gagcggcgga agctggccga catcatccac 5161 cactggaacg ccaaccggct ggacctgttc gagatcagcc agccgaccga ggatttggag 5221 ttccatggag tgatgagatt ttattttcaa gataaagctg ctggaaactt tgcaacaaaa 5281 tgtattcggg tctctagtac tgccaccact caagatgtaa tcgaaacgct cgcggagaaa 5341 tttcgacctg atatgcgaat gctgtcctct cccaagtatt cactctatga agtgcatgtc 5401 agcggagaaa gaagattgga tatagatgag aaacctctag ttgtacaact gaattggaac 5461 aaagatgatc gggaaggcag atttgttctt aagaatgaga atgacgccat tcctcctaag 5521 gctcaaagta atggacctga aaagcaggaa aaagaagggg ttatccagaa cttcaagaga 5581 actctctcaa agaaagaaaa gaaggaaaaa aagaagagag aaaaagaggc attgcgacag 5641 gcatctgata aagatgatag acctttccaa ggggaggatg ttgaaaattc tcgactggct 5701 gctgaggttt acaaagacat gccggaaacc agctttactc gaaccatttc taatcctgag 5761 gtggttatga aacgacggag gcagcaaaaa ttggaaaaga gaatgcagga atttcggagc 5821 tcagatgggc ggcctgattc aggtggaaca ttgagaattt atgcagatag tttaaaacca 5881 aatattccct acaagacaat cctgctgtct actacagatc ctgcagactt tgctgtggct 5941 gaagctttag agaagtatgg tctggaaaaa gaaaacccta aggattactg catcgcccgg 6001 gttatgcttc ctcctggagc ccagcattct gatgaaaagg gtgctaaaga aattattctt 6061 gatgatgatg agtgtccttt acaaatcttc agggaatggc caagtgacaa agggatttta 6121 gtctttcagt tgaagaggag gccaccagac cacatcccaa agaaaaccaa gaaacacttg 6181 gaaggcaaga cacccaaggg aaaggagaga gctgacgggt ctggctatgg ctccaccctt 6241 cctccggaga agctgcccta tttagtagag ttaagcccag atggttctga ctctagagat 6301 aagccaaagc tttaccgcct tcagttaagt gttactgaag ttgggacaga aaagttggat 6361 gacaactcta tccagttgtt tggcccagga attcagcccc atcactgtga ccttaccaac 6421 atggatggag tggtcactgt gacgcccaga agtatggacg cagaaaccta cgtggaaggc 6481 cagcgcatct cagaaaccac catgctgcag agtggcatga aagtgcagtt tggggcgtcc 6541 catgtattta agtttgtgga ccccagtcag gatcatgctc ttgcaaaaag atctgtggat 6601 ggaggcctga tggttaaggg cccaagacat aaacctggaa ttgttcagga gacaactttt 6661 gatttgggag gagatattca tagtgggaca gcattaccga caagcaagag caccactagg 6721 ctggacagcg acagagtgtc gtctgcctct agcacagccg agcggggaat ggtgaagccg 6781 atgatcagag tagaacagca gccagattat cgcaggcaag aaagcagaac acaggatgct 6841 tctgggcctg agctgatact acctgcaagc attgaattca gggaaagttc tgaagattca 6901 tttttgtctg ccattataaa ttatactaat agctctacag tccactttaa gttgtcccct 6961 acatatgtat tatatatggc atgccggtat gtattgtcca accagtacag acctgacatc 7021 agccctacag agcgcacaca taaagtcatt gcagtcgtca acaagatggt gagcatgatg 7081 gagggtgtca tccagaaaca gaagaatatt gcaggggcac ttgccttctg gatggcaaat 7141 gcatctgaac ttctcaactt cattaagcaa gaccgagacc ttagtcggat cacactggat 7201 gctcaagatg ttttagcaca tttggttcaa atggcattta aatacttggt tcactgtctt 7261 caatcagaac ttaataatta catgccagcc tttctagatg accctgaaga gaacagtctg 7321 caacgaccaa aaatagatga tgtgctgcac acgctcacag gagccatgtc cttgctacga 7381 cgctgcagag tcaatgccgc cctgaccatc cagctcttct ctcagctctt ccacttcatc 7441 aatatgtggc tgttcaatag attggtgacc gacccagatt cggggctgtg ctcccattac 7501 tggggtgcga ttatccgtca gcagttgggc catattgaag cctgggctga gaagcagggg 7561 ctggaactgg ctgcggactg tcatctgagc aggatcgtgc aggcaacgac tttgcttacc 7621 atggataagt atgcacctga tgacattcca aatataaaca gcacctgctt taagttaaat 7681 tcattacaac ttcaagcctt attacagaac tatcactgtg cacctgatga gccttttatc 7741 ccaacggatc ttatagaaaa tgtagtgact gtggctgaaa acactgccga tgagctggcc 7801 cgcagtgatg gaagggaagt gcagttggag gaggatcctg atctgcagct gccgtttctt 7861 ttgccagaag atggttattc ttgtgatgtt gtcagaaaca ttccaaatgg tttacaagaa 7921 tttttagacc ctctgtgcca gagaggattt tgcaggttaa ttcctcacac acgttcacca 7981 ggtacttgga caatatattt tgaaggtgca gattatgaaa gtcaccttct gcgtgagaac 8041 acagagctgg ctcagcctct gaggaaagaa cctgaaataa tcactgtgac cctaaaaaag 8101 cagaatggaa tgggccttag cattgttgca gcaaagggtg ctggtcaaga taaactagga 8161 atctatgtga agtcggttgt gaaaggaggt gctgcagatg atggacgtct agctgcaggt 8221 gatcagctcc tcagtgtgga tggacgaagt ctggttggac tctctcagga aagggcggca 8281 gaactcatga caagaacaag ctctgtggtg acactggaag tagcaaagca gggtgccatc 8341 taccacggtc tggccaccct tctcaatcag ccatccccca tgatgcagag aatttcagat 8401 cgtcgtggct caggtaaacc ccgaccaaag agtgaaggct ttgagctcta taataattca 8461 actcaaaatg ggtctcctga gagtcctcag ctgccttggg cagaatatag tgaaccaaag 8521 aaattgcctg gtgatgacag actgatgaaa aatagagctg atcaccgttc cagccccaac 8581 gtagcaaatc agcctcctag tcctggaggg aaaagtgcat atgcctctgg aacaacagcg 8641 aagataacat ctgtctctac tggaaacctc tgcactgagg agcagacgcc tccgcctaga 8701 cctgaagcct accccatccc cactcagacg tacaccagag agtattttac cttcccagct 8761 tccaaatccc aggatcggat ggctcctcct cagaaccagt ggccaaatta tgaggaaaag 8821 ccacatatgc acacagatag taatcattcc agtattgcaa ttcagcgtgt tacacgttcc 8881 caagaagaac ttcgagaaga taaagcttac caacttgagc ggcatcgaat agaggcagct 8941 atggaccgaa agtctgatag tgatatgtgg ataaatcaga gctcctcact ggactccagt 9001 acctctagcc aggagcatct gaaccattcc tctaagtcgg tcacccctgc ttccacactg 9061 accaaaagtg gccctggccg ttggaaaaca ccagcagcca taccggccac ccctgtggcc 9121 gtctcccagc caatccgaac agacctgcct ccgccacccc cgccacctcc agtccactat 9181 gccggtgatt tcgatggaat gtccatggat ttgcctctcc caccaccccc ttccgccaac 9241 cagatagggc tgccgtctgc gcaggtggct gctgctgaac ggagaaagag agaagaacat 9301 cagcgttggt atgagaagga gaaggcccgc ctggaggagg agcgggagag gaagcggaga 9361 gagcaggaga ggaagttggg ccagatgcgc actcagtcct taaaccctgc tccgttttct 9421 cccctgactg cacagcagat gaagcccgaa aagccttcca cactccagcg gccacaggaa 9481 acagtcattc gggagctgca gcctcagcag cagccccgca cgatcgagcg cagagacttg 9541 cagtacatta cagtcagcaa agaggagctt tcctcggggg acagtctgtc ccccgacccg 9601 tggaagcggg acgccaagga gaagctggag aagcagcagc agatgcacat cgtggacatg 9661 ctgagcaagg agatccagga gctccagagc aaaccggacc gcagcgccga ggagagcgac 9721 cggctgcgca agctcatgct ggagtggcag ttccagaaga gactccagga gtcgaagcag 9781 aaggacgaag atgacgagga ggaggaggac gatgatgtgg acaccatgct gatcatgcag 9841 cgcctggagg ctgaacgaag agcgaggttg caggacgagg agcggaggcg gcagcagcag 9901 ttagaagaga tgcgcaagcg ggaagcggaa gaccgagcga ggcaagagga agagcgccgg 9961 cggcaggagg aggagcgaac aaaacgagac gctgaagaaa agaggcgaca ggaagaaggg 10021 tattacagcc gcctggaagc cgagaggcgc agacagcacg acgaggcggc gcgcaggttg 10081 ctggagcccg aggcgcccgg accaaactct tacccaggat ctactggagc agctgttgga 10141 gcccatgacg cctgtcggga tgcaaaagag aagcgctcta aaagccaaga tgcagattca 10201 cctggaagtt ctggggcccc tgaaaacttg acattcaagg aacgccagcg tcttttttca 10261 caaggtcaag atgtatccaa taaagtgaaa gcttctcgta aattaacaga actggagaat 10321 gaactgaaca caaagtgaGC ggccgcggat cccgcccctc tccctccccc ccccctaacg 10381 ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca 10441 ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga 10501 gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga 10561 aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca 10621 ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag 10681 atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa 10741 gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac 10801 cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga 10861 ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac 10921 gatgataagc ttgccacaac ccacaaggag acgaccttcc atgaccgagt acaagcccac 10981 ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta cgcaccctcg ccgccgcgtt 11041 cgccgactac cccgccacgc gccacaccgt cgacccggac cgccacatcg agcgggtcac 11101 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc 11161 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt 11221 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca 11281 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac 11341 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg 11401 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa 11461 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg 11521 accgcgcacc tggtgcatga cccgcaagcc cggtgcctag acgcgtctgg aacaatcaac 11581 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta 11641 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt 11701 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg 11761 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg 11821 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca 11881 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca 11941 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg 12001 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag 12061 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc 12121 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt aattctgcag 12181 tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga 12241 ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt 12301 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa 12361 gaggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat 12421 ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc caggggtcag 12481 atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga 12541 agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga 12601 tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt 12661 ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg 12721 actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc 12781 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc 12841 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa 12901 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga 12961 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc 13021 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggccttgac 13081 attgctagcg tttaccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt 13141 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 13201 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 13261 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 13321 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 13381 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 13441 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 13501 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 13561 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 13621 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 13681 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 13741 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 13801 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 13861 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 13921 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 13981 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 14041 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 14101 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 14161 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 14221 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 14281 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 14341 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 14401 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 14461 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 14521 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 14581 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 14641 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 14701 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 14761 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 14821 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 14881 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 14941 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 15001 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 15061 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 15121 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 15181 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 15241 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag 15301 atcaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 15361 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 15421 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc 15481 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat 15541 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta 15601 gtagttttta aagaaattgt atttgttaaa tatgtactac aaacttagta gt

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.