pLV3-CMV-EGFP(159-238aa)-DNM1L

pLV3-CMV-EGFP(159-238aa)-DNM1L plasmid

PVT96255

pLV3-CMV-EGFP(159-238aa)-DNM1L plasmid Information

Plasmid name: pLV3-CMV-EGFP(159-238aa)-DNM1L(human)
Alias: NM_001278464.2;DLP1; DRP1; DVLP; DYMPLE; EMPF; EMPF1
Gene lenght: 2250bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-EGFP(159-238aa)-DNM1L plasmid Description

pLV3-CMV-EGFP(159-238aa)-DNM1L It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-EGFP(159-238aa)-DNM1L plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 1981 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 2041 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 2101 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 2161 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG 2221 TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT 2281 AGAGCTAGCG AATTCCTCGA GGCCACCATG ATGCTGAAAA AAATCCTGAA AATCGAAGAG 2341 CTGGATGAAC GTGAACTGAT CGATATTGAG GTGTCCGGTA ACCACCTGTT TTACGCTAAC 2401 GATATTCTGA CCCACAACTC ATCTTCAGAC GTTAACGGCA TCAAGGTGAA CTTCAAGATC 2461 CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC ACTACCAGCA GAACACCCCC 2521 ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCACCCA GTCCGCCCTG 2581 AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC 2641 GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGggaTCCG GCGGTGGAGG AAGCggcgga 2701 ggtggatctA TGGAGGCGCT AATTCCTGTC ATAAACAAGC TCCAGGACGT CTTCAACACG 2761 GTGGGCGCCG ACATCATCCA GCTGCCTCAA ATCGTCGTAG TGGGAACGCA GAGCAGCGGA 2821 AAGAGCTCAG TGCTAGAAAG CCTGGTGGGG AGGGACCTGC TTCCCAGAGG TACTGGAATT 2881 GTCACCCGGA GACCTCTCAT TCTGCAACTG GTCCATGTTT CACAAGAAGA TAAACGGAAA 2941 ACAACAGGAG AAGAAAATGA CCCTGCTACA TGGAAAAACT CAAGACACCT TTCTAAAGGG 3001 GTGGAAGCAG AAGAATGGGG TAAATTTCTT CACACCAAAA ATAAGCTTTA CACGGATTTT 3061 GATGAAATTC GACAAGAAAT TGAAAATGAA ACAGAAAGAA TTTCAGGAAA TAATAAGGGA 3121 GTAAGCCCTG AACCAATTCA TCTTAAGATT TTTTCACCCA ACGTTGTCAA TTTGACACTT 3181 GTGGATTTGC CAGGAATGAC CAAGGTGCCT GTAGGTGATC AACCTAAGGA TATTGAGCTT 3241 CAAATCAGAG AGCTCATTCT TCGGTTCATC AGTAATCCTA ATTCCATTAT CCTCGCTGTC 3301 ACTGCTGCTA ATACAGATAT GGCAACATCA GAGGCACTTA AAATTTCAAG AGAGGTAGAT 3361 CCAGATGGTC GCAGAACCCT AGCTGTAATC ACTAAACTTG ATCTCATGGA TGCGGGTACT 3421 GATGCCATGG ATGTATTGAT GGGAAGGGTT ATTCCAGTCA AACTTGGAAT AATTGGAGTA 3481 GTTAACAGGA GCCAGCTAGA TATTAACAAC AAGAAGAGTG TAACTGATTC AATCCGTGAT 3541 GAGTATGCTT TTCTTCAAAA GAAATATCCA TCTCTGGCCA ATAGAAATGG AACAAAGTAT 3601 CTTGCTAGGA CTCTAAACAG GTTACTGATG CATCACATCA GAGATTGTTT ACCAGAGTTG 3661 AAAACAAGAA TAAATGTTCT AGCTGCTCAG TATCAGTCTC TTCTAAATAG CTACGGTGAA 3721 CCCGTGGATG ATAAAAGTGC TACTTTACTC CAACTTATTA CCAAATTTGC CACAGAATAT 3781 TGTAACACTA TTGAAGGAAC TGCAAAATAT ATTGAAACTT CGGAGCTATG CGGTGGTGCT 3841 AGAATTTGTT ATATTTTCCA TGAGACTTTT GGGCGAACCT TAGAATCTGT TGATCCACTT 3901 GGTGGCCTTA ACACTATTGA CATTTTGACT GCCATTAGAA ATGCTACTGG TCCTCGTCCT 3961 GCTTTATTTG TGCCTGAGGT TTCATTTGAG TTACTGGTGA AGCGGCAAAT CAAACGTCTA 4021 GAAGAGCCCA GCCTCCGCTG TGTGGAACTG GTTCATGAGG AAATGCAAAG GATCATTCAG 4081 CACTGTAGCA ATTACAGTAC ACAGGAATTG TTACGATTTC CTAAACTTCA TGATGCCATA 4141 GTTGAAGTGG TGACTTGTCT TCTTCGTAAA AGGTTGCCTG TTACAAATGA AATGGTCCAT 4201 AACTTAGTGG CAATTGAACT GGCTTATATC AACACAAAAC ATCCAGACTT TGCTGATGCT 4261 TGTGGGCTAA TGAACAATAA TATAGAGGAA CAAAGGAGAA ACAGGCTAGC CAGAGAATTA 4321 CCTTCAGCTG TATCACGAGA CAAGTCTTCT AAAGTTCCAA GTGCTTTGGC ACCTGCCTCC 4381 CAGGAGCCCT CCCCCGCTGC TTCTGCTGAG GCTGATGGCA AGTTAATTCA GGACAGCAGA 4441 AGAGAAACTA AAAATGTTGC ATCTGGAGGT GGTGGGGTTG GAGATGGTGT TCAAGAACCA 4501 ACCACAGGCA ACTGGAGAGG AATGCTGAAA ACTTCAAAAG CTGAAGAGTT ATTAGCAGAA 4561 GAAAAATCAA AACCCATTCC AATTATGCCA GCCAGTCCAC AAAAAGGTCA TGCCGTGAAC 4621 CTGCTAGATG TGCCAGTTCC TGTTGCACGA AAACTATCTG CTCGGGAACA GCGAGATTGT 4681 GAGGTTATTG AACGACTCAT TAAATCATAT TTTCTCATTG TCAGAAAGAA TATTCAAGAC 4741 AGTGTGCCAA AGGCAGTAAT GCATTTTTTG GTTAATCATG TGAAAGACAC TCTTCAGAGT 4801 GAGCTAGTAG GCCAGCTGTA TAAATCATCC TTATTGGATG ATCTTCTGAC AGAATCTGAG 4861 GACATGGCAC AGCGCAGGAA AGAAGCAGCT GATATGCTAA AGGCATTACA AGGAGCCAGT 4921 CAAATTATTG CTGAAATCCG GGAGACTCAT CTTTGGTGAG CGGCCGCGTC GACAATCAAC 4981 CTCTGGATTA CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA 5041 CGCTATGTGG ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC CGTATGGCTT 5101 TCATTTTCTC CTCCTTGTAT AAATCCTGGT TGCTGTCTCT TTATGAGGAG TTGTGGCCCG 5161 TTGTCAGGCA ACGTGGCGTG GTGTGCACTG TGTTTGCTGA CGCAACCCCC ACTGGTTGGG 5221 GCATTGCCAC CACCTGTCAG CTCCTTTCCG GGACTTTCGC TTTCCCCCTC CCTATTGCCA 5281 CGGCGGAACT CATCGCCGCC TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA 5341 CTGACAATTC CGTGGTGTTG TCGGGGAAAT CATCGTCCTT TCCTTGGCTG CTCGCCTGTG 5401 TTGCCACCTG GATTCTGCGC GGGACGTCCT TCTGCTACGT CCCTTCGGCC CTCAATCCAG 5461 CGGACCTTCC TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT CTTCGCCTTC 5521 GCCCTCAGAC GAGTCGGATC TCCCTTTGGG CCGCCTCCCC GCCTGGTACC TTTAAGACCA 5581 ATGACTTACA AGGCAGCTGT AGATCTTAGC CACTTTTTAA AAGAAAAGGG GGGACTGGAA 5641 GGGCTAATTC ACTCCCAACG AAAATAAGAT CTGCTTTTTG CTTGTACTGG GTCTCTCTGG 5701 TTAGACCAGA TCTGAGCCTG GGAGCTCTCT GGCTAACTAG GGAACCCACT GCTTAAGCCT 5761 CAATAAAGCT TGCCTTGAGT GCTTCAAGTA GTGTGTGCCC GTCTGTTGTG TGACTCTGGT 5821 AACTAGAGAT CCCTCAGACC CTTTTAGTCA GTGTGGAAAA TCTCTAGCAG TAGTAGTTCA 5881 TGTCATCTTA TTATTCAGTA TTTATAACTT GCAAAGAAAT GAATATCAGA GAGTGAGAGG 5941 AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA 6001 AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT 6061 TATCATGTCT GGCTCTAGCT ATCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC 6121 CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG 6181 AGGCCGCCTC GGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG 6241 ACTTTTGCAG AGACCAAATT CGTAATCATG TCATAGCTGT TTCCTGTGTG AAATTGTTAT 6301 CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC 6361 TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA 6421 AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT 6481 ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG 6541 CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC 6601 GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG 6661 TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA 6721 AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC 6781 TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC 6841 CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG 6901 GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC 6961 TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA 7021 GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG 7081 AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG 7141 AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT 7201 GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA 7261 GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA 7321 GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA 7381 TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC 7441 TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA 7501 CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA 7561 ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC 7621 GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT 7681 TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC 7741 ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT 7801 TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC 7861 TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG 7921 GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT 7981 GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG 8041 GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA 8101 AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG 8161 TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG 8221 TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT 8281 TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 8341 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 8401 TTTCCCCGAA AAGTGCCACC TGACGTCTAA GAAACCATTA TTATCATGAC ATTAACCTAT 8461 AAAAATAGGC GTATCACGAG GCCCTTTCGT CTCGCGCGTT TCGGTGATGA CGGTGAAAAC 8521 CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC 8581 AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG GCTTAACTAT 8641 GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATATGC GGTGTGAAAT ACCGCACAGA 8701 TGCGTAAGGA GAAAATACCG CATCAGGCGC CATTCGCCAT TCAGGCTGCG CAACTGTTGG 8761 GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT 8821 GCAAGGCGAT TAAGTTGGGT AACGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG 8881 GCCAGTGCCA AGCTG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.