pCMV-SEC23A-3×Myc-Neo plasmid

pCMV-SEC23A-3×Myc-Neo plasmid

PVT96212

pCMV-SEC23A-3×Myc-Neo plasmid Information

Plasmid name: pCMV-SEC23A(human)-3×Myc-Neo
Alias: NM_006364.4;CLSD; hSec23A
Gene lenght: 2295bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-SEC23A-3×Myc-Neo plasmid Description

pCMV-SEC23A-3×Myc-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-SEC23A-3×Myc-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGTTTAAAC TTAAGCTTGG 661 TACCGAGCTC GGATCCACTA GTCCAGTGTG GTGGAATTCG CCACCatgac aacctatttg 721 gaattcattc aacaaaatga agaacgagat ggagtccgat ttagttggaa tgtttggcca 781 tcaagtcgac tggaagctac aagaatggtt gttcctgtgg cagccctgtt tacaccactg 841 aaagagagac ctgacttacc acctattcaa tatgaacctg ttctgtgtag taggaccact 901 tgccgtgcag ttttgaatcc tttatgtcaa gtggattatc gagcaaaact ttgggcttgc 961 aacttttgtt accaaaggaa tcagtttcca cctagttatg ctggtatatc tgaactgaat 1021 cagcctgctg aacttttacc tcagttttct agcattgaat atgtagttct gcgtggtcct 1081 cagatgcctt tgatattcct ctatgtggtt gatacttgca tggaagatga agatttacaa 1141 gccctgaaag aatccatgca gatgtcatta agtcttttac cacctacagc tttggttgga 1201 cttattactt ttgggagaat ggttcaggtt catgaacttg gatgtgaagg catttcaaaa 1261 agctatgtct tcagaggaac aaaagatttg tctgccaaac aactgcagga aatgctgggg 1321 ctctctaaag taccacttac tcaagcaaca cgtggtcctc aggtacagca gccacctcct 1381 tccaacagat tcttacaacc agtacagaaa atagacatga atctcacaga tcttctggga 1441 gaactccagc gagacccttg gcctgtacca cagggaaaga gacctttgcg ttcctctggg 1501 gtggcacttt ccatagctgt aggactgctg gagtgtactt ttcccaacac tggtgctcgt 1561 atcatgatgt tcattggtgg tcctgctact caggggcctg gaatggtggt tggagatgag 1621 ttgaagacac ctataagatc gtggcatgac attgacaaag acaatgccaa atatgttaaa 1681 aagggaacta agcattttga agcattggct aatcgagctg ctacaactgg ccatgttatt 1741 gatatctatg cgtgtgcatt agatcagaca ggtctcctgg agatgaaatg ctgtcccaac 1801 cttactggag gatacatggt aatgggtgat tctttcaata cttccttatt caaacaaact 1861 tttcaaagag tctttaccaa agacatgcat ggacagttta aaatgggctt tggtggtacg 1921 ctagaaataa agacctcaag ggaaataaag atttcaggag ctattggacc ctgtgtgtca 1981 ctcaattcta aaggaccctg tgtgtctgaa aatgagatag gaacaggtgg cacatgtcag 2041 tggaagatat gtggacttag tcccactaca accttagcca tatattttga ggttgtcaat 2101 cagcataatg ctccaattcc tcaaggaggg cgtggtgcaa tccagtttgt gactcagtat 2161 cagcattcaa gtgggcagag acgcatccga gtgaccacca ttgctaggaa ctgggcagat 2221 gctcaaactc aaatccaaaa cattgctgca tcttttgacc aggaggcagc tgccattctt 2281 atggcccggc tagcaatata tagagcagaa acagaagaag gtccagatgt gcttaggtgg 2341 ctggacagac agctcattcg actgtgtcag aaatttggag aatatcataa agatgaccca 2401 agttccttca gattttcaga aactttctcc ctttatccac agtttatgtt tcatttaaga 2461 agatcttctt tcctgcaagt ttttaacaat agtcctgatg agagttcata ttatcgtcac 2521 cattttatgc gtcaagatct gacccagtct ctaattatga ttcagcctat cctgtatgcg 2581 tattctttta gtggaccacc agagccggtt cttcttgata gcagtagcat tcttgcagat 2641 cgtattcttc tcatggacac attcttccag attttgattt atcatggtga gaccatagca 2701 cagtggcgga agtcaggata ccaggatatg cctgagtatg aaaatttccg ccaccttctg 2761 caagccccag tggatgatgc acaggaaatt cttcactcca gatttccaat gccaagatac 2821 attgacactg aacatggagg cagccaggcc cgtttcctcc tttcaaaagt caacccttca 2881 cagactcata ataatatgta tgcctggggg caggagtctg gagcacctat tcttacagat 2941 gatgttagtt tacaagtgtt tatggatcac ttgaagaaac ttgctgtgtc cagtgctgct 3001 CTCGAGGAAC AAAAACTAAT ATCAGAGGAA GATTTGGAAC AGAAACTAAT CAGTGAAGAA 3061 GATTTAGAGC AAAAGTTAAT TTCCGAGGAG GACTTATAGT GAGGGCCCGA TATCTCGACA 3121 ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG TATTCTTAAC TATGTTGCTC 3181 CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA TCATGCTATT GCTTCCCGTA 3241 TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT 3301 GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT TGCTGACGCA ACCCCCACTG 3361 GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA 3421 TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG CTGGACAGGG GCTCGGCTGT 3481 TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAGCTGAC GTCCTTTCCA TGGCTGCTCG 3541 CCTGTGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG CTACGTCCCT TCGGCCCTCA 3601 ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC 3661 GCCTTCGCCC TCAGACGAGT CGGATCTCCC TTTGGGCCGC CTCCCCGCCT GGAAACGGGG 3721 GAGGCTAACT GAAACACGGA AGGAGACAAT ACCGGAAGGA ACCCGCGCTA TGACGGCAAT 3781 AAAAAGACAG AATAAAACGC ACGGGTGTTG GGTCGTTTGT TCATAAACGC GGGGTTCGGT 3841 CCCAGGGCTG GCACTCTGTC GATACCCCAC CGAGACCCCA TTGGGGCCAA TACGCCCGCG 3901 TTTCTTCCTT TTCCCCACCC CACCCCCCAA GTTCGGGTGA AGGCCCAGGG CTCGCAGCCA 3961 ACGTCGGGGC GGCAGGCCCT GCCATAGCAG ATCTGCGCAG CTGGGGCTCT AGGGGGTATC 4021 CCCACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA 4081 CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG 4141 CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG CATCCCTTTA GGGTTCCGAT 4201 TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG 4261 GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA 4321 GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT 4381 TATAAGGGAT TTTGGGGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT 4441 TTAACGCGAA TTAATTCTGT GGAATGTGTG TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC 4501 CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA GGTGTGGAAA 4561 GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC 4621 CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC 4681 TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCTGCCTC 4741 TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT 4801 CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAAGAG ACAGGATGAG GATCGTTTCG 4861 CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT 4921 CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC 4981 AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT 5041 GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT 5101 GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA 5161 GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT 5221 GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG 5281 CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA 5341 AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA 5401 CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA 5461 TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA 5521 CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT 5581 CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT 5641 TGACGAGTTC TTCTGAGCGG GACTCTGGGG TTCGCGAAAT GACCGACCAA GCGACGCCCA 5701 ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA 5761 TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG CTGGAGTTCT 5821 TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA 5881 CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA 5941 TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG GCGTAATCAT 6001 GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG 6061 CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG 6121 CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA 6181 TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA 6241 CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG 6301 TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC 6361 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC 6421 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC 6481 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC 6541 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCAAT 6601 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC 6661 ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA 6721 ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG 6781 CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA 6841 GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG 6901 GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC 6961 AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT 7021 CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA 7081 GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT 7141 ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA 7201 TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC 7261 GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG 7321 CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG 7381 CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT 7441 CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT 7501 CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT 7561 CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA 7621 AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA 7681 TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT 7741 AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC 7801 ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA 7861 GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT 7921 CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG 7981 CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT 8041 ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT 8101 AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCG 8161 ACGGATCGGG AGATCTCCCG ATCCCCTATG GTCGACTCTC AGTACAATCT GCTCTGATGC 8221 CGCATAGTTA AGCCAGTATC TGCTCCCTGC TTGTGTGTTG GAGGTCGCTG AGTAGTGCGC 8281 GAGCAAAATT TAAGCTACAA CAAGGCAAGG CTTGACCGAC AATTGCATGA AGAATCTGCT 8341 TAGG

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.