pSTM1787-luxCDABE plasmid

pSTM1787-luxCDABE plasmid

PVT96172

pSTM1787-luxCDABE plasmid Information

Plasmid name: pSTM1787-luxCDABE
Alias: -
Gene lenght: 6679bp
Host: E.coli
Use(s): Protein Expression
Fragment type: ORF
Species:
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: STM1787
Replicon: pBR322
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: araC-F1
Reverse primer:
Copy number: High
Induction:

pSTM1787-luxCDABE plasmid Description

pSTM1787-luxCDABE is a plasmid suitable for protein expression in Escherichia coli.

pSTM1787-luxCDABE plasmid Sequence

1 atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac 61 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca 121 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta 181 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata 241 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag 301 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag 361 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg 421 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct 481 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc 541 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc 601 gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca 661 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga 721 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa 781 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata 841 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc 901 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt 961 tgcgcttcag ccatactttt catactcccg ccattcagag acgccctctt tcaaacagtc 1021 tcttttttgc attcctttaa aaccagcatc actattttat ataaaaatca tcacgaagta 1081 tgcttctttt aacgatgacc tcaaatcctc cccccttttg catcaactta cgcatccctg 1141 aaatggcgag aacaggctaa atctacccga ggtcactcgc taaaaacctc atcctggaac 1201 aagctcaacc gcccttcccc gctacggccc tttcgccgaa aaaccgataa tgggtataaa 1261 tataccgcta taagctatac ccacacactt cacattgttt cttatcgtta atccgatacg 1321 cttttttcac attatttctg gcggtatacg ccggtgcaaa gtgaatcggg ctcgtttccg 1381 cgctcgcgcg taacacaatg atttaccttg ctggcggaac gtcaacggac ccgcaaaagc 1441 gccttatgcc cctggcggag cgtggtgcaa aaaggagaaa aaatacgcgt tatgaataac 1501 gaggagacct tttatcaagc tctagaaata attttgttta actttaagaa ggagatatac 1561 atATGACTAA AAAAATTTCA TTCATTATTA ACGGCCAGGT TGAAATCTTT CCCGAAGGTG 1621 ATGATTTAGT GCAATCCATT AATTTTGGTG ATAATAGTGT TTACCTGCCA ATATTGAATG 1681 ACTCTCATGT AAAAAACATT ATTGATTGTA ATGGAAATAA CGAATTACGG TTGCATAACA 1741 TTGTCAATTT TCTCTATACG GTAGGGCAAA GATGGAAAAA TGAAGAATAC TCAAGACGCA 1801 GGACATACAT TCGTGACTTA AAAAAATATA TGGGATATTC AGAAGAAATG GCTAAGCTAG 1861 AGGCCAATTG GATATCTATG ATTTTATGTT CTAAAGGCGG CCTTTATGAT GTTGTAGAAA 1921 ATGAACTTGG TTCTCGCCAT ATCATGGATG AATGGCTACC TCAGGATGAA AGTTATGTTC 1981 GGGCTTTTCC GAAAGGTAAA TCTGTACATC TGTTGGCAGG TAATGTTCCA TTATCTGGGA 2041 TCATGTCTAT ATTACGCGCA ATTTTAACTA AGAATCAGTG TATTATAAAA ACATCGTCAA 2101 CCGATCCTTT TACCGCTAAT GCATTAGCGT TAAGTTTTAT TGATGTAGAC CCTAATCATC 2161 CGATAACGCG CTCTTTATCT GTTATATATT GGCCCCACCA AGGTGATACA TCACTCGCAA 2221 AAGAAATTAT GCAACATGCG GATGTTATTG TCGCTTGGGG AGGGCCAGAT GCGATTAATT 2281 GGGCGGTAGA GCATGCGCCA TCTTATGCTG ATGTGATTAA ATTTGGTTCT AAAAAGAGTC 2341 TTTGCATTAT CGATAATCCT GTTGATTTGA CGTCCGCAGC GACAGGTGCG GCTCATGATG 2401 TTTGTTTTTA CGATCAGCGA GCTTGTTTTT CTGCCCAAAA CATATATTAC ATGGGAAATC 2461 ATTATGAGGA ATTTAAGTTA GCGTTGATAG AAAAACTTAA TCTATATGCG CATATATTAC 2521 CGAATGCCAA AAAAGATTTT GATGAAAAGG CGGCCTATTC TTTAGTTCAA AAAGAAAGCT 2581 TGTTTGCTGG ATTAAAAGTA GAGGTGGATA TTCATCAACG TTGGATGATT ATTGAGTCAA 2641 ATGCAGGTGT GGAATTTAAT CAACCACTTG GCAGATGTGT GTACCTTCAT CACGTCGATA 2701 ATATTGAGCA AATATTGCCT TATGTTCAAA AAAATAAGAC GCAAACCATA TCTATTTTTC 2761 CTTGGGAGTC ATCATTTAAA TATCGAGATG CGTTAGCATT AAAAGGTGCG GAAAGGATTG 2821 TAGAAGCAGG AATGAATAAC ATATTTCGAG TTGGTGGATC TCATGACGGA ATGAGACCGT 2881 TGCAACGATT AGTGACATAT ATTTCTCATG AAAGGCCATC TAACTATACG GCTAAGGATG 2941 TTGCGGTTGA AATAGAACAG ACTCGATTCC TGGAAGAAGA TAAGTTCCTT GTATTTGTCC 3001 CATAATAGGT AAAAAGTATG GAAAATGAAT CAAAATATAA AACCATCGAC CACGTTATTT 3061 GTGTTGAAGG AAATAAAAAA ATTCATGTTT GGGAAACGCT GCCAGAAGAA AACAGCCCAA 3121 AGAGAAAGAA TGCCATTATT ATTGCGTCTG GTTTTGCCCG CAGGATGGAT CATTTTGCTG 3181 GTCTGGCGGA ATATTTATCG CGGAATGGAT TTCATGTGAT CCGCTATGAT TCGCTTCACC 3241 ACGTTGGATT GAGTTCAGGG ACAATTGATG AATTTACAAT GTCTATAGGA AAGCAGAGCT 3301 TGTTAGCAGT GGTTGATTGG TTAACTACAC GAAAAATAAA TAACTTCGGT ATGTTGGCTT 3361 CAAGCTTATC TGCGCGGATA GCTTATGCAA GCCTATCTGA AATCAATGCT TCGTTTTTAA 3421 TCACCGCAGT CGGTGTTGTT AACTTAAGAT ATTCTCTTGA AAGAGCTTTA GGGTTTGATT 3481 ATCTCAGTCT ACCCATTAAT GAATTGCCGA ATAATCTAGA TTTTGAAGGC CATAAATTGG 3541 GTGCTGAAGT CTTTGCGAGA GATTGTCTTG ATTTTGGTTG GGAAGATTTA GCTTCTACAA 3601 TTAATAACAT GATGTATCTT GATATACCGT TTATTGCTTT TACTGCAAAT AACGATAATT 3661 GGGTCAAGCA AGATGAAGTT ATCACATTGT TATCAAATAT TCGTAGTAAT CGATGCAAGA 3721 TATATTCTTT GTTAGGAAGT TCGCATGACT TGAGTGAAAA TTTAGTGGTC CTGCGCAATT 3781 TTTATCAATC GGTTACGAAA GCCGCTATCG CGATGGATAA TGATCATCTG GATATTGATG 3841 TTGATATTAC TGAACCGTCA TTTGAACATT TAACTATTGC GACAGTCAAT GAACGCCGAA 3901 TGAGAATTGA GATTGAAAAT CAAGCAATTT CTCTGTCTTA AAATCTATTG AGATATTCTA 3961 TCACTCAAAT AGCAATATAA GGACTCTCTA TGAAATTTGG AAACTTTTTG CTTACATACC 4021 AACCTCCCCA ATTTTCTCAA ACAGAGGTAA TGAAACGTTT GGTTAAATTA GGTCGCATCT 4081 CTGAGGAGTG TGGTTTTGAT ACCGTATGGT TACTGGAGCA TCATTTCACG GAGTTTGGTT 4141 TGCTTGGTAA CCCTTATGTC GCTGCTGCAT ATTTACTTGG CGCGACTAAA AAATTGAATG 4201 TAGGAACTGC CGCTATTGTT CTTCCCACAG CCCATCCAGT ACGCCAACTT GAAGATGTGA 4261 ATTTATTGGA TCAAATGTCA AAAGGACGAT TTCGGTTTGG TATTTGCCGA GGGCTTTACA 4321 ACAAGGACTT TCGCGTATTC GGCACAGATA TGAATAACAG TCGCGCCTTA GCGGAATGCT 4381 GGTACGGGCT GATAAAGAAT GGCATGACAG AGGGATATAT GGAAGCTGAT AATGAACATA 4441 TCAAGTTCCA TAAGGTAAAA GTAAACCCCG CGGCGTATAG CAGAGGTGGC GCACCGGTTT 4501 ATGTGGTGGC TGAATCAGCT TCGACGACTG AGTGGGCTGC TCAATTTGGC CTACCGATGA 4561 TATTAAGTTG GATTATAAAT ACTAACGAAA AGAAAGCACA ACTTGAGCTT TATAATGAAG 4621 TGGCTCAAGA ATATGGGCAC GATATTCATA ATATCGACCA TTGCTTATCA TATATAACAT 4681 CTGTAGATCA TGACTCAATT AAAGCGAAAG AGATTTGCCG GAAATTTCTG GGGCATTGGT 4741 ATGATTCTTA TGTGAATGCT ACGACTATTT TTGATGATTC AGACCAAACA AGAGGTTATG 4801 ATTTCAATAA AGGGCAGTGG CGTGACTTTG TATTAAAAGG ACATAAAGAT ACTAATCGCC 4861 GTATTGATTA CAGTTACGAA ATCAATCCCG TGGGAACGCC GCAGGAATGT ATTGACATAA 4921 TTCAAAAAGA CATTGATGCT ACAGGAATAT CAAATATTTG TTGTGGATTT GAAGCTAATG 4981 GAACAGTAGA CGAAATTATT GCTTCCATGA AGCTCTTCCA GTCTGATGTC ATGCCATTTC 5041 TTAAAGAAAA ACAACGTTCG CTATTATATT AGCTAAGGAG AAAGAAATGA AATTTGGATT 5101 GTTCTTCCTT AACTTCATCA ATTCAACAAC TGTTCAAGAA CAAAGTATAG TTCGCATGCA 5161 GGAAATAACG GAGTATGTTG ATAAGTTGAA TTTTGAACAG ATTTTAGTGT ATGAAAATCA 5221 TTTTTCAGAT AATGGTGTTG TCGGCGCTCC TCTGACTGTT TCTGGTTTTC TGCTCGGTTT 5281 AACAGAGAAA ATTAAAATTG GTTCATTAAA TCACATCATT ACAACTCATC ATCCTGTCCG 5341 CATAGCGGAG GAAGCTTGCT TATTGGATCA GTTAAGTGAA GGGAGATTTA TTTTAGGGTT 5401 TAGTGATTGC GAAAAAAAAG ATGAAATGCA TTTTTTTAAT CGCCCGGTTG AATATCAACA 5461 GCAACTATTT GAAGAGTGTT ATGAAATCAT TAACGATGCT TTAACAACAG GCTATTGTAA 5521 TCCAGATAAC GATTTTTATA GCTTCCCTAA AATATCTGTA AATCCCCATG CTTATACGCC 5581 AGGCGGACCT CGGAAATATG TAACAGCAAC CAGTCATCAT ATTGTTGAGT GGGCGGCCAA 5641 AAAAGGTATT CCTCTCATCT TTAAGTGGGA TGATTCTAAT GATGTTAGAT ATGAATATGC 5701 TGAAAGATAT AAAGCCGTTG CGGATAAATA TGACGTTGAC CTATCAGAGA TAGACCATCA 5761 GTTAATGATA TTAGTTAACT ATAACGAAGA TAGTAATAAA GCTAAACAAG AGACGCGTGC 5821 ATTTATTAGT GATTATGTTC TTGAAATGCA CCCTAATGAA AATTTCGAAA ATAAACTTGA 5881 AGAAATAATT GCAGAAAACG CTGTCGGAAA TTATACGGAG TGTATAACTG CGGCTAAGTT 5941 GGCAATTGAA AAGTGTGGTG CGAAAAGTGT ATTGCTGTCC TTTGAACCAA TGAATGATTT 6001 GATGAGCCAA AAAAATGTAA TCAATATTGT TGATGATAAT ATTAAGAAGT ACCACATGGA 6061 ATATACCTAA TAGATTTCGA GTTGCAGCGA GGCGGCAAGT GAACGAATCC CCAGGAGCAT 6121 AGATAACTAT GTGACTGGGG TGAGTGAAAG CAGCCAACAA AGCAGCAGCT TGAAAGATGA 6181 AGGGTATAAA AGAGTATGAC AGCAGTGCTG CCATACTTTC TAATATTATC TTGAGGAGTA 6241 AAACAGGTAT GACTTCATAT GTTGATAAAC AAGAAATTAC AGCAAGCTCA GAAATTGATG 6301 ATTTGATTTT TTCGAGCGAT CCATTAGTGT GGTCTTACGA CGAGCAGGAA AAAATCAGAA 6361 AGAAACTTGT GCTTGATGCA TTTCGTAATC ATTATAAACA TTGTCGAGAA TATCGTCACT 6421 ACTGTCAGGC ACACAAAGTA GATGACAATA TTACGGAAAT TGATGACATA CCTGTATTCC 6481 CAACATCGGT TTTTAAGTTT ACTCGCTTAT TAACTTCTCA GGAAAACGAG ATTGAAAGTT 6541 GGTTTACCAG TAGCGGCACG AATGGTTTAA AAAGTCAGGT GGCGCGTGAC AGATTAAGTA 6601 TTGAGAGACT CTTAGGCTCT GTGAGTTATG GCATGAAATA TGTTGGTAGT TGGTTTGATC 6661 ATCAAATAGA ATTAGTCAAT TTGGGACCAG ATAGATTTAA TGCTCATAAT ATTTGGTTTA 6721 AATATGTTAT GAGTTTGGTG GAATTGTTAT ATCCTACGAC ATTTACCGTA ACAGAAGAAC 6781 GAATAGATTT TGTTAAAACA TTGAATAGTC TTGAACGAAT AAAAAATCAA GGGAAAGATC 6841 TTTGTCTTAT TGGTTCGCCA TACTTTATTT ATTTACTCTG CCATTATATG AAAGATAAAA 6901 AAATCTCATT TTCTGGAGAT AAAAGCCTTT ATATCATAAC CGGAGGCGGC TGGAAAAGTT 6961 ACGAAAAAGA ATCTCTGAAA CGTGATGATT TCAATCATCT TTTATTTGAT ACTTTCAATC 7021 TCAGTGATAT TAGTCAGATC CGAGATATAT TTAATCAAGT TGAACTCAAC ACTTGTTTCT 7081 TTGAGGATGA AATGCAGCGT AAACATGTTC CGCCGTGGGT ATATGCGCGA GCGCTTGATC 7141 CTGAAACGTT GAAACCTGTA CCTGATGGAA CGCCGGGGTT GATGAGTTAT ATGGATGCGT 7201 CAGCAACCAG TTATCCAGCA TTTATTGTTA CCGATGATGT CGGGATAATT AGCAGAGAAT 7261 ATGGTAAGTA TCCCGGCGTG CTCGTTGAAA TTTTACGTCG CGTCAATACG AGGACGCAGA 7321 AAGGGTGTGC TTTAAGCTTA ACCGAAGCGT TTGATAGTTG Aaagcttggc tgttttggcg 7381 gatgagagaa gattttcagc ctgatacaga ttaaatcaga acgcagaagc ggtctgataa 7441 aacagaattt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg ccgaactcag 7501 aagtgaaacg ccgtagcgcc gatggtagtg tggggtctcc ccatgcgaga gtagggaact 7561 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt 7621 tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgggagcgga tttgaacgtt 7681 gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc cataaactgc caggcatcaa 7741 attaagcaga aggccatcct gacggatggc ctttttgcgt ttctacaaac tcttttgttt 7801 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct 7861 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc 7921 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa 7981 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg 8041 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt 8101 tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg 8161 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac 8221 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc 8281 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa 8341 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc 8401 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt 8461 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga 8521 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa 8581 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa 8641 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa 8701 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt 8761 ttactcatat atactttaga ttgatttacg cgccctgtag cggcgcatta agcgcggcgg 8821 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt 8881 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc 8941 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg 9001 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga 9061 cgttggagtc cacgttcttt aatagtggac tcttgttcca aacttgaaca acactcaacc 9121 ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa 9181 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa 9241 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt 9301 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 9361 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 9421 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga 9481 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac 9541 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt 9601 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag 9661 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 9721 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag 9781 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 9841 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt 9901 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc 9961 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc 10021 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc 10081 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat 10141 tttctcctta cgcatctgtg cggtatttca caccgcatag ggtcatggct gcgccccgac 10201 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca 10261 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga 10321 aacgcgcgag gcagcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc 10381 atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg 10441 gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg 10501 atgcgtccgg cgtagaggat ctgctcatgt ttgacagctt atc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.