pLV2-CMV-FUT7-3×FLAG-Puro plas

pLV2-CMV-FUT7-3×FLAG-Puro plasmid

PVT90196

pLV2-CMV-FUT7-3×FLAG-Puro plasmid Information

Plasmid name: pLV2-CMV-FUT7(human)-3×FLAG-Puro
Alias: NM_004479.4;FucT-VII;
Gene lenght: 1026bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-FUT7-3×FLAG-Puro plasmid Description

pLV2-CMV-FUT7-3×FLAG-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-CMV-FUT7-3×FLAG-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcGCCA CCatgaataa tgctgggcac ggccccaccc ggaggctgcg 2881 aggcttgggg gtcctggccg gggtggctct gctcgctgcc ctctggctcc tgtggctgct 2941 ggggtcagcc cctcggggta ccccggcacc ccagcccacg atcaccatcc ttgtctggca 3001 ctggcccttc actgaccagc ccccagagct gcccagcgac acctgcaccc gctacggcat 3061 cgcccgctgc cacctgagtg ccaaccgaag cctgctggcc agcgccgacg ccgtggtctt 3121 ccaccaccgc gagctgcaga cccggcggtc ccacctgccc ctggcccagc ggccgcgagg 3181 gcagccctgg gtgtgggcct ccatggagtc tcctagccac acccacggcc tcagccacct 3241 ccgaggcatc ttcaactggg tgctgagcta ccggcgcgac tcggacatct ttgtgcccta 3301 tggccgcctg gagccccact gggggccctc gccaccgctg ccagccaaga gcagggtggc 3361 cgcctgggtg gtcagcaact tccaggagcg gcagctgcgt gccaggctgt accggcagct 3421 ggcgcctcat ctgcgggtgg atgtctttgg ccgtgccaat ggacggccac tgtgcgccag 3481 ctgcctggtg cccaccgtgg cccagtaccg cttctacctg tcctttgaga actctcagca 3541 ccgcgactac attacggaga aattctggcg caacgcactg gtggctggca ctgtgccagt 3601 ggtgctgggg cccccacggg ccacctatga ggccttcgtg ccggctgacg ccttcgtgca 3661 tgtggatgac tttggctcag cccgagagct ggcggctttc ctcactggca tgaatgagag 3721 ccgataccaa cgcttctttg cctggcgtga caggctccgc gtgcgactgt tcaccgactg 3781 gcgggaacgt ttctgtgcca tctgtgaccg ctacccacac ctaccccgca gccaagtcta 3841 tgaggacctt gagggttggt ttcaggccGG TGGAGGCgga tccGACTACA AAGACCATGA 3901 CGGTGATTAT AAAGATCATG ACATCGACTA CAAGGATGAC GATGACAAGt agtctagata 3961 attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc 4021 cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac 4081 cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc 4141 cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga 4201 agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt 4261 aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct 4321 gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg 4381 aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt 4441 ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat gaccgagtac 4501 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc 4561 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag 4621 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg 4681 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg 4741 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc 4801 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc 4861 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg 4921 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg 4981 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg 5041 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacc gcgtctggaa 5101 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc 5161 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg 5221 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt 5281 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac 5341 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc 5401 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct 5461 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct 5521 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct 5581 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct 5641 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattaa 5701 ttctgcagtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc 5761 aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca 5821 cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta 5881 aaagaaaaga ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat 5941 ctgtggatct accacacaca aggctacttc cctgattagc agaactacac accagggcca 6001 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat 6061 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat 6121 gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt 6181 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc 6241 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag 6301 tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg 6361 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc 6421 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg 6481 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc 6541 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag 6601 gccttgacat tgctagcgtt ttaccgtcga cctctagcta gagcttggcg taatcatggt 6661 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 6721 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 6781 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg 6841 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 6901 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 6961 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 7021 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 7081 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 7141 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 7201 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 7261 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 7321 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 7381 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 7441 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 7501 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 7561 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 7621 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 7681 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 7741 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 7801 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 7861 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 7921 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 7981 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 8041 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 8101 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 8161 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 8221 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 8281 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 8341 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 8401 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 8461 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 8521 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 8581 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 8641 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 8701 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 8761 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg 8821 gatcgggaga tcaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 8881 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 8941 atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc 9001 aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa 9061 acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact 9121 acaaacttag tagtttttaa agaaattgta tttgttaaat atgtactaca aacttagtag 9181 t

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.