pLV2-CMV-TXNDC12-3×FLAG-Puro p

pLV2-CMV-TXNDC12-3×FLAG-Puro plasmid

PVT96136

pLV2-CMV-TXNDC12-3×FLAG-Puro plasmid Information

Plasmid name: pLV2-CMV-TXNDC12(human)-3×FLAG-Puro
Alias: NM_015913.4;AG1; AGR1; ERP16; ERP18; ERP19; hAG-1; hTLP19; PDIA16; TLP19
Gene lenght: 516bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-TXNDC12-3×FLAG-Puro plasmid Description

pLV2-CMV-TXNDC12-3×FLAG-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-CMV-TXNDC12-3×FLAG-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcGCCA CCatggagac gcggcctcgt ctcggggcca cctgtttgct 2881 gggcttcagt ttcctgctcc tcgtcatctc ttctgatgga cataatgggc ttggaaaggg 2941 ttttggagat catattcatt ggaggacact ggaagatggg aagaaagaag cagctgccag 3001 tggactgccc ctgatggtga ttattcataa atcctggtgt ggagcttgca aagctctaaa 3061 gcccaaattt gcagaatcta cggaaatttc agaactctcc cataattttg ttatggtaaa 3121 tcttgaggat gaagaggaac ccaaagatga agatttcagc cctgacgggg gttatattcc 3181 acgaatcctt tttctggatc ccagtggcaa ggtgcatcct gaaatcatca atgagaatgg 3241 aaaccccagc tacaagtatt tttatgtcag tgccgagcaa gttgttcagg ggatgaagga 3301 agctcaggaa aggctgacgg gtgatgcctt cagaaagaaa catcttgaag atgaattggg 3361 atccGACTAC AAAGACCATG ACGGTGATTA TAAAGATCAT GACATCGACT ACAAGGATGA 3421 CGATGACAAG tagtctagat aattctaccg ggtaggggag gcgcttttcc caaggcagtc 3481 tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc 3541 tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt 3601 cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc 3661 gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac 3721 cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt 3781 cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct 3841 caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa 3901 gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcagccc 3961 aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc 4021 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc 4081 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc 4141 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc 4201 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag 4261 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg 4321 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag 4381 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc 4441 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc 4501 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc 4561 ggtgcctgac cgcgtctgga acaatcaacc tctggattac aaaatttgtg aaagattgac 4621 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt 4681 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt 4741 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt 4801 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg 4861 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg 4921 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct 4981 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt 5041 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc 5101 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc 5161 cgcctccccg cctggaatta attctgcagt cgagacctag aaaaacatgg agcaatcaca 5221 agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag 5281 gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct 5341 gtagatctta gccacttttt aaaagaaaag aggggactgg aagggctaat tcactcccaa 5401 cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattag 5461 cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag 5521 ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg 5581 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg 5641 tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag 5701 aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt 5761 ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt 5821 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact 5881 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc 5941 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa 6001 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa 6061 atgaatatca gagagtgaga ggccttgaca ttgctagcgt tttaccgtcg acctctagct 6121 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa 6181 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga 6241 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 6301 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 6361 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 6421 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 6481 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 6541 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 6601 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 6661 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 6721 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 6781 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 6841 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 6901 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 6961 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta 7021 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 7081 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 7141 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 7201 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 7261 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 7321 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 7381 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 7441 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 7501 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 7561 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 7621 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 7681 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 7741 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 7801 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 7861 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 7921 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 7981 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 8041 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 8101 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 8161 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 8221 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 8281 aagtgccacc tgacgtcgac ggatcgggag atcaacttgt ttattgcagc ttataatggt 8341 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct 8401 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact 8461 caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac 8521 ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat 8581 tgtatttgtt aaatatgtac tacaaactta gtagttttta aagaaattgt atttgttaaa 8641 tatgtactac aaacttagta gt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.