pLV3-CMV-EGFP-HOXA2-3×FLAG-Pur

pLV3-CMV-EGFP-HOXA2-3×FLAG-Puro plasmid

PVT91621

pLV3-CMV-EGFP-HOXA2-3×FLAG-Puro plasmid Information

Plasmid name: pLV3-CMV-EGFP-HOXA2(human)-3×FLAG-Puro
Alias: NM_006735.4;HOX1K; MCOHI;
Gene lenght: 1128bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: pEGFP-C-5'
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-EGFP-HOXA2-3×FLAG-Puro plasmid Description

pLV3-CMV-EGFP-HOXA2-3×FLAG-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-EGFP-HOXA2-3×FLAG-Puro plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 1981 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 2041 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 2101 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 2161 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG 2221 TGACCGTCAG ATCGCCTGGA GACGCCATCC ACGCTGTTTT GACCTCCATA GAAGATTCTA 2281 GAGCTAGCGA ATTCagccac catggtgagc aagggcgagg agctgttcac cggggtggtg 2341 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag 2401 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag 2461 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc 2521 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac 2581 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg 2641 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag 2701 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc 2761 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag 2821 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc 2881 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac 2941 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc 3001 atggacgagc tgtacaagGG AGGAGGGGGA AGCggtggag gcggttctCT CGAGatgaat 3061 tacgaatttg agcgagagat tggttttatc aatagccagc cgtcgctcgc tgagtgcctg 3121 acatcttttc cccctgtcgc tgatacattt caaagttcat caatcaagac ctcgacgctt 3181 tcacactcga cactgattcc tcctcctttt gagcagacca ttcccagcct gaaccccggc 3241 agtcaccctc gccacggcgc tggcggccgc cccaagccga gccccgcggg cagccgcggc 3301 agcccggtgc ccgccggcgc cctgcagccg cccgagtacc cctggatgaa ggagaagaag 3361 gcggccaaga aaaccgcact tctgccggcc gccgccgccg ccgccaccgc cgcagccacc 3421 ggccctgctt gcctcagcca caaagaatcc ctggaaatcg ccgatggcag cggcggggga 3481 tcgcggcgcc tgagaactgc ttacaccaac acacagcttc tagagctgga aaaagaattt 3541 catttcaaca agtacctttg cagaccccga agggtggaga ttgcagcgct gctggatttg 3601 actgagagac aagtgaaagt gtggtttcag aaccggagga tgaagcacaa gaggcagacc 3661 cagtgcaagg aaaaccaaaa cagcgaaggg aaatgtaaaa gccttgagga ctccgagaaa 3721 gtagaggagg acgaggaaga gaagacgctc tttgagcaag cccttagcgt ctctggggcc 3781 cttctggaga gggaaggcta cacttttcag caaaatgccc tctctcagca gcaggctccc 3841 aatggacaca atggcgactc ccaaagtttc ccagtctcgc ctttaaccag caatgagaaa 3901 aatctgaaac attttcagca ccagtcaccc actgttccca actgcttgtc aacaatgggc 3961 cagaactgtg gagctggcct aaacaatgac agtcctgagg cccttgaggt cccctctttg 4021 caggacttta gcgttttctc cacagattcc tgcctgcagc tttcagatgc agtttcaccc 4081 agtttgccag gttccctcga cagtcccgta gatatttcag ctgacagctt agactttttt 4141 acagacacac tcaccacaat cgacttgcag catctgaatt acGGATCCGA CTACAAAGAC 4201 CATGACGGTG ATTATAAAGA TCATGACATC GACTACAAGG ATGACGATGA CAAGTAGCGG 4261 CCGCGAAGGA TCTGCGATCG CTCCGGTGCC CGTCAGTGGG CAGAGCGCAC ATCGCCCACA 4321 GTCCCCGAGA AGTTGGGGGG AGGGGTCGGC AATTGAACGG GTGCCTAGAG AAGGTGGCGC 4381 GGGGTAAACT GGGAAAGTGA TGTCGTGTAC TGGCTCCGCC TTTTTCCCGA GGGTGGGGGA 4441 GAACCGTATA TAAGTGCAGT AGTCGCCGTG AACGTTCTTT TTCGCAACGG GTTTGCCGCC 4501 AGAACACAGC TGAAGCTTCG AGGGGCTCGC ATCTCTCCTT CACGCGCCCG CCGCCCTACC 4561 TGAGGCCGCC ATCCACGCCG GTTGAGTCGC GTTCTGCCGC CTCCCGCCTG TGGTGCCTCC 4621 TGAACTGCGT CCGCCGTCTA GGTAAGTTTA AAGCTCAGGT CGAGACCGGG CCTTTGTCCG 4681 GCGCTCCCTT GGAGCCTACC TAGACTCAGC CGGCTCTCCA CGCTTTGCCT GACCCTGCTT 4741 GCTCAACTCT ACGTCTTTGT TTCGTTTTCT GTTCTGCGCC GTTACAGATC CAAGCTGTGA 4801 CCGGCGCCTA CGCTAGATGA CCGAGTACAA GCCCACGGTG CGCCTCGCCA CCCGCGACGA 4861 CGTCCCCCGG GCCGTACGCA CCCTCGCCGC CGCGTTCGCC GACTACCCCG CCACGCGCCA 4921 CACCGTCGAC CCGGACCGCC ACATCGAGCG GGTCACCGAG CTGCAAGAAC TCTTCCTCAC 4981 GCGCGTCGGG CTCGACATCG GCAAGGTGTG GGTCGCGGAC GACGGCGCCG CGGTGGCGGT 5041 CTGGACCACG CCGGAGAGCG TCGAAGCGGG GGCGGTGTTC GCCGAGATCG GCCCGCGCAT 5101 GGCCGAGTTG AGCGGTTCCC GGCTGGCCGC GCAGCAACAG ATGGAAGGCC TCCTGGCGCC 5161 GCACCGGCCC AAGGAGCCCG CGTGGTTCCT GGCCACCGTC GGCGTCTCGC CCGACCACCA 5221 GGGCAAGGGT CTGGGCAGCG CCGTCGTGCT CCCCGGAGTG GAGGCGGCCG AGCGCGCCGG 5281 GGTGCCCGCC TTCCTGGAGA CCTCCGCGCC CCGCAACCTC CCCTTCTACG AGCGGCTCGG 5341 CTTCACCGTC ACCGCCGACG TCGAGGTGCC CGAAGGACCG CGCACCTGGT GCATGACCCG 5401 CAAGCCCGGT GCCTGAGTCG ACAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC 5461 TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT TAATGCCTTT 5521 GTATCATGCT ATTGCTTCCC GTATGGCTTT CATTTTCTCC TCCTTGTATA AATCCTGGTT 5581 GCTGTCTCTT TATGAGGAGT TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG TGTGCACTGT 5641 GTTTGCTGAC GCAACCCCCA CTGGTTGGGG CATTGCCACC ACCTGTCAGC TCCTTTCCGG 5701 GACTTTCGCT TTCCCCCTCC CTATTGCCAC GGCGGAACTC ATCGCCGCCT GCCTTGCCCG 5761 CTGCTGGACA GGGGCTCGGC TGTTGGGCAC TGACAATTCC GTGGTGTTGT CGGGGAAATC 5821 ATCGTCCTTT CCTTGGCTGC TCGCCTGTGT TGCCACCTGG ATTCTGCGCG GGACGTCCTT 5881 CTGCTACGTC CCTTCGGCCC TCAATCCAGC GGACCTTCCT TCCCGCGGCC TGCTGCCGGC 5941 TCTGCGGCCT CTTCCGCGTC TTCGCCTTCG CCCTCAGACG AGTCGGATCT CCCTTTGGGC 6001 CGCCTCCCCG CCTGGTACCT TTAAGACCAA TGACTTACAA GGCAGCTGTA GATCTTAGCC 6061 ACTTTTTAAA AGAAAAGGGG GGACTGGAAG GGCTAATTCA CTCCCAACGA AAATAAGATC 6121 TGCTTTTTGC TTGTACTGGG TCTCTCTGGT TAGACCAGAT CTGAGCCTGG GAGCTCTCTG 6181 GCTAACTAGG GAACCCACTG CTTAAGCCTC AATAAAGCTT GCCTTGAGTG CTTCAAGTAG 6241 TGTGTGCCCG TCTGTTGTGT GACTCTGGTA ACTAGAGATC CCTCAGACCC TTTTAGTCAG 6301 TGTGGAAAAT CTCTAGCAGT AGTAGTTCAT GTCATCTTAT TATTCAGTAT TTATAACTTG 6361 CAAAGAAATG AATATCAGAG AGTGAGAGGA ACTTGTTTAT TGCAGCTTAT AATGGTTACA 6421 AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT 6481 GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG GCTCTAGCTA TCCCGCCCCT 6541 AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC 6601 AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG 6661 AGGCCTAGAC TTTTGCAGAG ACGGCCCAAA TTCGTAATCA TGGTCATAGC TGTTTCCTGT 6721 GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA 6781 AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC 6841 TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG 6901 AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT 6961 CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 7021 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 7081 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 7141 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 7201 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 7261 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 7321 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 7381 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 7441 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 7501 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT 7561 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 7621 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 7681 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 7741 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 7801 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA 7861 CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC 7921 CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG 7981 CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT 8041 AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT 8101 CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG 8161 CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC 8221 ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA 8281 AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC 8341 ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT 8401 TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG 8461 TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT 8521 GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG 8581 ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC 8641 CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC 8701 GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA 8761 GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG 8821 GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC TAAGAAACCA TTATTATCAT 8881 GACATTAACC TATAAAAATA GGCGTATCAC GAGGCCCTTT CGTCTCGCGC GTTTCGGTGA 8941 TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG GTCACAGCTT GTCTGTAAGC 9001 GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG 9061 CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA TGCGGTGTGA 9121 AATACCGCAC AGATGCGTAA GGAGAAAATA CCGCATCAGG CGCCATTCGC CATTCAGGCT 9181 GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA 9241 AGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG 9301 TTGTAAAACG ACGGCCAGTG CCAAGCTG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.