pLV3-CMV-EP300-3×FLAG-Hyg plas

pLV3-CMV-EP300-3×FLAG-Hyg plasmid

PVT96129

pLV3-CMV-EP300-3×FLAG-Hyg plasmid Information

Plasmid name: pLV3-CMV-EP300(human)-3×FLAG-Hyg
Alias: NM_001429.4;KAT3B; MKHK2; p300; RSTS2
Gene lenght: 7242bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Hyg
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-EP300-3×FLAG-Hyg plasmid Description

pLV3-CMV-EP300-3×FLAG-Hyg It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-EP300-3×FLAG-Hyg plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcc GGATCCGCCA CCatggccga gaatgtggtg gaaccggggc cgccttcagc 2341 caagcggcct aaactctcat ctccggccct ctcggcgtcc gccagcgatg gcacagattt 2401 tggctctcta tttgacttgg agcacgactt accagatgaa ttaatcaact ctacagaatt 2461 gggactaacc aatggtggtg atattaatca gcttcagaca agtcttggca tggtacaaga 2521 tgcagcttct aaacataaac agctgtcaga attgctgcga tctggtagtt cccctaacct 2581 caatatggga gttggtggcc caggtcaagt catggccagc caggcccaac agagcagtcc 2641 tggattaggt ttgataaata gcatggtcaa aagcccaatg acacaggcag gcttgacttc 2701 tcccaacatg gggatgggca ctagtggacc aaatcagggt cctacgcagt caacaggtat 2761 gatgaacagt ccagtaaatc agcctgccat gggaatgaac acagggatga atgcgggcat 2821 gaatcctgga atgttggctg caggcaatgg acaagggata atgcctaatc aagtcatgaa 2881 cggttcaatt ggagcaggcc gagggcgaca gaatatgcag tacccaaacc caggcatggg 2941 aagtgctggc aacttactga ctgagcctct tcagcagggc tctccccaga tgggaggaca 3001 aacaggattg agaggccccc agcctcttaa gatgggaatg atgaacaacc ccaatcctta 3061 tggttcacca tatactcaga atcctggaca gcagattgga gccagtggcc ttggtctcca 3121 gattcagaca aaaactgtac tatcaaataa cttatctcca tttgctatgg acaaaaaggc 3181 agttcctggt ggaggaatgc ccaacatggg tcaacagcca gccccgcagg tccagcagcc 3241 aggcctggtg actccagttg cccaagggat gggttctgga gcacatacag ctgatccaga 3301 gaagcgcaag ctcatccagc agcagcttgt tctccttttg catgctcaca agtgccagcg 3361 ccgggaacag gccaatgggg aagtgaggca gtgcaacctt ccccactgtc gcacaatgaa 3421 gaatgtccta aaccacatga cacactgcca gtcaggcaag tcttgccaag tggcacactg 3481 tgcatcttct cgacaaatca tttcacactg gaagaattgt acaagacatg attgtcctgt 3541 gtgtctcccc ctcaaaaatg ctggtgataa gagaaatcaa cagccaattt tgactggagc 3601 acccgttgga cttggaaatc ctagctctct aggggtgggt caacagtctg cccccaacct 3661 aagcactgtt agtcagattg atcccagctc catagaaaga gcctatgcag ctcttggact 3721 accctatcaa gtaaatcaga tgccgacaca accccaggtg caagcaaaga accagcagaa 3781 tcagcagcct gggcagtctc cccaaggcat gcggcccatg agcaacatga gtgctagtcc 3841 tatgggagta aatggaggtg taggagttca aacgccgagt cttctttctg actcaatgtt 3901 gcattcagcc ataaattctc aaaacccaat gatgagtgaa aatgccagtg tgccctccct 3961 gggtcctatg ccaacagcag ctcaaccatc cactactgga attcggaaac agtggcacga 4021 agatattact caggatcttc gaaatcatct tgttcacaaa ctcgtccaag ccatatttcc 4081 tacgccggat cctgctgctt taaaagacag acggatggaa aacctagttg catatgctcg 4141 gaaagttgaa ggggacatgt atgaatctgc aaacaatcga gcggaatact accaccttct 4201 agctgagaaa atctataaga tccagaaaga actagaagaa aaacgaagga ccagactaca 4261 gaagcagaac atgctaccaa atgctgcagg catggttcca gtttccatga atccagggcc 4321 taacatggga cagccgcaac caggaatgac ttctaatggc cctctacctg acccaagtat 4381 gatccgtggc agtgtgccaa accagatgat gcctcgaata actccacaat ctggtttgaa 4441 tcaatttggc cagatgagca tggcccagcc ccctattgta ccccggcaaa cccctcctct 4501 tcagcaccat ggacagttgg ctcaacctgg agctctcaac ccgcctatgg gctatgggcc 4561 tcgtatgcaa cagccttcca accagggcca gttccttcct cagactcagt tcccatcaca 4621 gggaatgaat gtaacaaata tccctttggc tccgtccagc ggtcaagctc cagtgtctca 4681 agcacaaatg tctagttctt cctgcccggt gaactctcct ataatgcctc cagggtctca 4741 ggggagccac attcactgtc cccagcttcc tcaaccagct cttcatcaga attcaccctc 4801 gcctgtacct agtcgtaccc ccacccctca ccatactccc ccaagcatag gggctcagca 4861 gccaccagca acaacaattc cagcccctgt tcctacacct cctgccatgc cacctgggcc 4921 acagtcccag gctctacatc cccctccaag gcagacacct acaccaccaa caacacaact 4981 tccccaacaa gtgcagcctt cacttcctgc tgcaccttct gctgaccagc cccagcagca 5041 gcctcgctca cagcagagca cagcagcgtc tgttcctacc ccaacagcac cgctgcttcc 5101 tccgcagcct gcaactccac tttcccagcc agctgtaagc attgaaggac aggtatcaaa 5161 tcctccatct actagtagca cagaagtgaa ttctcaggcc attgctgaga agcagccttc 5221 ccaggaagtg aagatggagg ccaaaatgga agtggatcaa ccagaaccag cagatactca 5281 gccggaggat atttcagagt ctaaagtgga agactgtaaa atggaatcta ccgaaacaga 5341 agagagaagc actgagttaa aaactgaaat aaaagaggag gaagaccagc caagtacttc 5401 agctacccag tcatctccgg ctccaggaca gtcaaagaaa aagattttca aaccagaaga 5461 actacgacag gcactgatgc caactttgga ggcactttac cgtcaggatc cagaatccct 5521 tccctttcgt caacctgtgg accctcagct tttaggaatc cctgattact ttgatattgt 5581 gaagagcccc atggatcttt ctaccattaa gaggaagtta gacactggac agtatcagga 5641 gccctggcag tatgtcgatg atatttggct tatgttcaat aatgcctggt tatataaccg 5701 gaaaacatca cgggtataca aatactgctc caagctctct gaggtctttg aacaagaaat 5761 tgacccagtg atgcaaagcc ttggatactg ttgtggcaga aagttggagt tctctccaca 5821 gacactgtgt tgctacggca aacagttgtg cacaatacct cgtgatgcca cttattacag 5881 ttaccagaac aggtatcatt tctgtgagaa gtgtttcaat gagatccaag gggagagcgt 5941 ttctttgggg gatgaccctt cccagcctca aactacaata aataaagaac aattttccaa 6001 gagaaaaaat gacacactgg atcctgaact gtttgttgaa tgtacagagt gcggaagaaa 6061 gatgcatcag atctgtgtcc ttcaccatga gatcatctgg cctgctggat tcgtctgtga 6121 tggctgttta aagaaaagtg cacgaactag gaaagaaaat aagttttctg ctaaaaggtt 6181 gccatctacc agacttggca cctttctaga gaatcgtgtg aatgactttc tgaggcgaca 6241 gaatcaccct gagtcaggag aggtcactgt tagagtagtt catgcttctg acaaaaccgt 6301 ggaagtaaaa ccaggcatga aagcaaggtt tgtggacagt ggagagatgg cagaatcctt 6361 tccataccga accaaagccc tctttgcctt tgaagaaatt gatggtgttg acctgtgctt 6421 ctttggcatg catgttcaag agtatggctc tgactgccct ccacccaacc agaggagagt 6481 atacatatct tacctcgata gtgttcattt cttccgtcct aaatgcttga ggactgcagt 6541 ctatcatgaa atcctaattg gatatttaga atatgtcaag aaattaggtt acacaacagg 6601 gcatatttgg gcatgtccac caagtgaggg agatgattat atcttccatt gccatcctcc 6661 tgaccagaag atacccaagc ccaagcgact gcaggaatgg tacaaaaaaa tgcttgacaa 6721 ggctgtatca gagcgtattg tccatgacta caaggatatt tttaaacaag ctactgaaga 6781 tagattaaca agtgcaaagg aattgcctta tttcgagggt gatttctggc ccaatgttct 6841 ggaagaaagc attaaggaac tggaacagga ggaagaagag agaaaacgag aggaaaacac 6901 cagcaatgaa agcacagatg tgaccaaggg agacagcaaa aatgctaaaa agaagaataa 6961 taagaaaacc agcaaaaata agagcagcct gagtaggggc aacaagaaga aacccgggat 7021 gcccaatgta tctaacgacc tctcacagaa actatatgcc accatggaga agcataaaga 7081 ggtcttcttt gtgatccgcc tcattgctgg ccctgctgcc aactccctgc ctcccattgt 7141 tgatcctgat cctctcatcc cctgcgatct gatggatggt cgggatgcgt ttctcacgct 7201 ggcaagggac aagcacctgg agttctcttc actccgaaga gcccagtggt ccaccatgtg 7261 catgctggtg gagctgcaca cgcagagcca ggaccgcttt gtctacacct gcaatgaatg 7321 caagcaccat gtggagacac gctggcactg tactgtctgt gaggattatg acttgtgtat 7381 cacctgctat aacactaaaa accatgacca caaaatggag aaactaggcc ttggcttaga 7441 tgatgagagc aacaaccagc aggctgcagc cacccagagc ccaggcgatt ctcgccgcct 7501 gagtatccag cgctgcatcc agtctctggt ccatgcttgc cagtgtcgga atgccaattg 7561 ctcactgcca tcctgccaga agatgaagcg ggttgtgcag cataccaagg gttgcaaacg 7621 gaaaaccaat ggcgggtgcc ccatctgcaa gcagctcatt gccctctgct gctaccatgc 7681 caagcactgc caggagaaca aatgcccggt gccgttctgc ctaaacatca agcagaagct 7741 ccggcagcaa cagctgcagc accgactaca gcaggcccaa atgcttcgca ggaggatggc 7801 cagcatgcag cggactggtg tggttgggca gcaacagggc ctcccttccc ccactcctgc 7861 cactccaacg acaccaactg gccaacagcc aaccaccccg cagacgcccc agcccacttc 7921 tcagcctcag cctacccctc ccaatagcat gccaccctac ttgcccagga ctcaagctgc 7981 tggccctgtg tcccagggta aggcagcagg ccaggtgacc cctccaaccc ctcctcagac 8041 tgctcagcca ccccttccag ggcccccacc tgcagcagtg gaaatggcaa tgcagattca 8101 gagagcagcg gagacgcagc gccagatggc ccacgtgcaa atttttcaaa ggccaatcca 8161 acaccagatg cccccgatga ctcccatggc ccccatgggt atgaacccac ctcccatgac 8221 cagaggtccc agtgggcatt tggagccagg gatgggaccg acagggatgc agcaacagcc 8281 accctggagc caaggaggat tgcctcagcc ccagcaacta cagtctggga tgccaaggcc 8341 agccatgatg tcagtggccc agcatggtca acctttgaac atggctccac aaccaggatt 8401 gggccaggta ggtatcagcc cactcaaacc aggcactgtg tctcaacaag ccttacaaaa 8461 ccttttgcgg actctcaggt ctcccagctc tcccctgcag cagcaacagg tgcttagtat 8521 ccttcacgcc aacccccagc tgttggctgc attcatcaag cagcgggctg ccaagtatgc 8581 caactctaat ccacaaccca tccctgggca gcctggcatg ccccaggggc agccagggct 8641 acagccacct accatgccag gtcagcaggg ggtccactcc aatccagcca tgcagaacat 8701 gaatccaatg caggcgggcg ttcagagggc tggcctgccc cagcagcaac cacagcagca 8761 actccagcca cccatgggag ggatgagccc ccaggctcag cagatgaaca tgaaccacaa 8821 caccatgcct tcacaattcc gagacatctt gagacgacag caaatgatgc aacagcagca 8881 gcaacaggga gcagggccag gaataggccc tggaatggcc aaccataacc agttccagca 8941 accccaagga gttggctacc caccacagca gcagcagcgg atgcagcatc acatgcaaca 9001 gatgcaacaa ggaaatatgg gacagatagg ccagcttccc caggccttgg gagcagaggc 9061 aggtgccagt ctacaggcct atcagcagcg actccttcag caacagatgg ggtcccctgt 9121 tcagcccaac cccatgagcc cccagcagca tatgctccca aatcaggccc agtccccaca 9181 cctacaaggc cagcagatcc ctaattctct ctccaatcaa gtgcgctctc cccagcctgt 9241 cccttctcca cggccacagt cccagccccc ccactccagt ccttccccaa ggatgcagcc 9301 tcagccttct ccacaccacg tttccccaca gacaagttcc ccacatcctg gactggtagc 9361 tgcccaggcc aaccccatgg aacaagggca ttttgccagc ccggaccaga attcaatgct 9421 ttctcagctt gctagcaatc caggcatggc aaacctccat ggtgcaagcg ccacggacct 9481 gggactcagc accgataact cagacttgaa ttcaaacctc tcacagagta cactagacat 9541 acacCTCGAG GACTACAAAG ACCATGACGG TGATTATAAA GATCATGACA TCGACTACAA 9601 GGATGACGAT GACAAGTAGc ggccgcaagg atctgcgatc gctccggtgc ccgtcagtgg 9661 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg 9721 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc 9781 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt 9841 tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct 9901 tcacgcgccc gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg 9961 cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg 10021 tcgagaccgg gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc 10081 acgctttgcc tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc 10141 cgttacagat ccaagctgtg accggcgcct acgtcgacat gaaaaagcct gaactcaccg 10201 cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc 10261 tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc 10321 tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg 10381 catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggaattcagc gagagcctga 10441 cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac 10501 tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta 10561 gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc 10621 gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg 10681 acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact 10741 gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca 10801 atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg 10861 aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct 10921 acttcgagcg gaggcatccg gagcttgcag gatcgccgcg gctccgggcg tatatgctcc 10981 gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt 11041 gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac 11101 aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata 11161 gtggaaaccg acgccccagc actcgtccga gggcaaagga ataggtcgac aatcaacctc 11221 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc 11281 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca 11341 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg 11401 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca 11461 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg 11521 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg 11581 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg 11641 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg 11701 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc 11761 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggtaccttt aagaccaatg 11821 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg 11881 ctaattcact cccaacgaaa ataagatctg ctttttgctt gtactgggtc tctctggtta 11941 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa 12001 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac 12061 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt 12121 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac 12181 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 12241 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 12301 catgtctggc tctagctatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc 12361 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat 12421 tccagaagta gtgaggaggc ttttttggag gcctagactt ttgcagagac ggcccaaatt 12481 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca 12541 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca 12601 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc 12661 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt 12721 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 12781 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 12841 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 12901 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 12961 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 13021 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 13081 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 13141 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 13201 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 13261 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 13321 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 13381 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 13441 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 13501 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 13561 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 13621 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta 13681 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa 13741 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac 13801 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa 13861 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag 13921 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg 13981 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag 14041 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg 14101 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc 14161 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat 14221 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata 14281 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa 14341 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca 14401 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc 14461 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc 14521 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg 14581 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac 14641 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga 14701 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc 14761 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg 14821 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg 14881 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc 14941 gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg 15001 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg 15061 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.