pCMV-ARHGAP4-mCherry-Neo plasm

pCMV-ARHGAP4-mCherry-Neo plasmid

PVT96113

pCMV-ARHGAP4-mCherry-Neo plasmid Information

Plasmid name: pCMV-ARHGAP4(human)-mCherry-Neo
Alias: NM_001666.5；C1; p115; RGC1; RhoGAP4; SrGAP4
Gene lenght: 2838bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-ARHGAP4-mCherry-Neo plasmid Description

pCMV-ARHGAP4-mCherry-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-ARHGAP4-mCherry-Neo plasmid Sequence

1 CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT 61 GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA 121 ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC 181 AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA 241 CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC 301 CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG 361 ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG 421 GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT 481 ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA 541 CCGGACTCAG ATCTCGAGCT CAAGCTTCGA ATTCgccacc atggccgctc acgggaagct 601 gcggcgggag cgggggctgc aggctgagta tgagacgcaa gtcaaagaga tgcgctggca 661 gctgagcgag cagctgcgct gcctggagct gcagggcgag ctgcggcggg agttgctgca 721 ggagctggca gagttcatgc ggcgccgcgc tgaggtggag ctggaatact cccggggcct 781 ggaaaagctg gccgagcgct tctccagccg tggaggccgc ctggggagca gccgggagca 841 ccaaagcttc cggaaggagc cgtccctcct gtcgcccttg cactgctggg cggtgctgct 901 gcagcacacg cggcagcaga gccgggagag cgcggccctg agtgaggtgc tggccgggcc 961 cctggcccag cgcctgagtc acattgcaga ggacgtgggg cgcctggtca agaagagcag 1021 ggatctggag cagcagctgc aggatgagct cctggaggtg gtctcagagc tccagacggc 1081 caagaagacg taccaggcat atcacatgga gagcgtgaat gccgaggcca agctccggga 1141 ggccgagcgg caggaggaga agcgggcagg ccggagtgtc cccaccacca ccgctggtgc 1201 cactgaggca gggcccctcc gcaagagctc cctcaagaag ggagggaggc tggtggagaa 1261 gcggcaggcc aagttcatgg agcacaaact caagtgcaca aaggcgcgca acgagtacct 1321 gcttagcctg gctagtgtca acgctgctgt cagtaactac tacctgcatg acgtcttgga 1381 cctcatggac tgctgtgaca cagggttcca cctggccctg gggcaggtgc tccggagcta 1441 cacggccgct gagagccgca cccaagcctc ccaagtgcag ggcctgggca gcctggaaga 1501 agctgtggag gccctggatc ctccagggga caaagccaag gttctcgagg tgcatgctac 1561 cgtcttctgt cccccgctgc gctttgacta ccacccccat gatggggatg aggtggctga 1621 gatctgcgtt gaaatggagc tgcgggacga gattctgccc agagcccaga acatccagag 1681 ccgcctggac cgacagacca ttgagacaga ggaggtgaac aagactctga aggcgacact 1741 gcaggccctg ctggaggtgg tggcctcgga tgacggggat gtgcttgatt ccttccagac 1801 cagcccctcc accgagtccc tcaagtccac cagctcagac ccaggcagcc ggcaggcggg 1861 ccggaggcgc ggccagcagc aggagaccga aaccttctac ctcacgaagc tccaggagta 1921 tctgagtgga cggagcatcc tcgccaagct gcaggccaag cacgagaagc tgcaggaggc 1981 ccttcagcga ggtgacaagg aggagcagga ggtgtcttgg acccagtaca cacagagaaa 2041 attccagaag agccgccagc cccgccccag ctcccagtat aaccagagac tctttggggg 2101 agacatggag aagtttatcc agagctcagg ccagcctgtg cccctggtgg tggagagctg 2161 cattcgcttc atcaacctca atggcctgca gcatgaaggc atcttccggg tatcgggtgc 2221 ccagctccgg gtctcagaga tccgtgatgc cttcgagaga ggggaggacc cactggtgga 2281 gggctgcact gcccatgacc tggactcggt ggccggggtg ctgaagctct acttccggag 2341 cctggagccc ccactcttcc ccccagacct gttcggcgag ctgctggctt cttcggagct 2401 ggaggccaca gcggagaggg tggagcacgt gagccgcctg ctgtggcggc tgcccgcgcc 2461 ggtgctggtg gttctgcgct acctcttcac cttcctcaac cacctggccc agtacagcga 2521 tgagaacatg atggacccct acaacctggc cgtgtgcttc gggcccacgc tgctaccggt 2581 gcccgctggg caggacccgg tggcgctgca gggccgggtg aaccagctgg tgcagacgct 2641 catagtgcag cccgatcggg tcttcccgcc cctgacctcg ctgcctggcc ccgtctacga 2701 gaagtgcatg gcaccgcctt ccgccagctg cctgggggac gcccagctgg agagcctggg 2761 ggcggacaat gagccggagc tggaagccga gatgcccgca caggaggatg acctggaggg 2821 ggtcgtggag gctgtggcct gctttgccta cacgggccgc acagcccagg agctgagctt 2881 ccggcggggg gacgtactgc ggctgcacga gagggcctcg agcgactggt ggcgggggga 2941 gcacaacggc atgcggggcc tcatccccca caagtatatc acgctgcccg ccgggacgga 3001 gaagcaggtg gtgggcgcag ggctgcagac tgcaggggag tctgggagca gtcccgaggg 3061 cctcctggca tcggagctgg tccaccggcc agagccatgc acctcacctg aggccatggg 3121 accctctgga cacagacgac gctgcttggt cccagcctcc ccagagcaac acgtggaggt 3181 ggataaggct gtggcacaga acatggactc tgtgtttaag gagctcttgg gaaagacctc 3241 tgtccgccag ggccttgggc cagcatctac cacctctccc agtcctgggc cccgaagccc 3301 aaaggccccg cccagcagcc gcctgggcag gaacaaaggc ttctcccggg gccctggggc 3361 cccagcctca ccctcagctt cccaccccca gggcctagac acgaccccca agccacacgg 3421 tggaggcgga tcaggaggcg gtggatctAT GGTGAGCAAG GGCGAGGAGG ATAACATGGC 3481 CATCATCAAG GAGTTCATGC GCTTCAAGGT GCACATGGAG GGCTCCGTGA ACGGCCACGA 3541 GTTCGAGATC GAGGGCGAGG GCGAGGGCCG CCCCTACGAG GGCACCCAGA CCGCCAAGCT 3601 GAAGGTGACC AAGGGTGGCC CCCTGCCCTT CGCCTGGGAC ATCCTGTCCC CTCAGTTCAT 3661 GTACGGCTCC AAGGCCTACG TGAAGCACCC CGCCGACATC CCCGACTACT TGAAGCTGTC 3721 CTTCCCCGAG GGCTTCAATT GGGAGCGCGT GATGAACTTC GAGGACGGCG GCGTGGTGAC 3781 CGTGACCCAG GACTCCTCCC TGCAGGACGG CGAGTTCATC TACAAGGTGA AGCTGCGCGG 3841 CACCAACTTC CCCTCCGACG GCCCCGTAAT GCAGTGTCGT ACCATGGGCT GGGAGGCCTC 3901 CACTGAGCGG ATGTACCCCG AGGACGGCGC CCTGAAGGGC GAGATCAAGC AGAGGCTGAA 3961 GCTGAAGGAC GGCGGCCACT ACGACGCTGA GGTCAAGACC ACCTACAAGG CCAAGAAGCC 4021 CGTGCAGCTG CCCGGCGCCT ACAACGTCGA CATCAAGTTG GACATCCTTT CCCACAACGA 4081 GGACTACACC ATCGTGGAAC AGTACGAACG CGCCGAGGGC CGCCACTCCA CCGGCGGCAT 4141 GGACGAGCTG TACAAGTAAG CGGCCGCGAC TCTAGATCAT AATCAGCCAT ACCACATTTG 4201 TAGAGGTTTT ACTTGCTTTA AAAAACCTCC CACACCTCCC CCTGAACCTG AAACATAAAA 4261 TGAATGCAAT TGTTGTTGTT AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA 4321 ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT 4381 CCAAACTCAT CAATGTATCT TAAGGCGTAA ATTGTAAGCG TTAATATTTT GTTAAAATTC 4441 GCGTTAAATT TTTGTTAAAT CAGCTCATTT TTTAACCAAT AGGCCGAAAT CGGCAAAATC 4501 CCTTATAAAT CAAAAGAATA GACCGAGATA GGGTTGAGTG TTGTTCCAGT TTGGAACAAG 4561 AGTCCACTAT TAAAGAACGT GGACTCCAAC GTCAAAGGGC GAAAAACCGT CTATCAGGGC 4621 GATGGCCCAC TACGTGAACC ATCACCCTAA TCAAGTTTTT TGGGGTCGAG GTGCCGTAAA 4681 GCACTAAATC GGAACCCTAA AGGGAGCCCC CGATTTAGAG CTTGACGGGG AAAGCCGGCG 4741 AACGTGGCGA GAAAGGAAGG GAAGAAAGCG AAAGGAGCGG GCGCTAGGGC GCTGGCAAGT 4801 GTAGCGGTCA CGCTGCGCGT AACCACCACA CCCGCCGCGC TTAATGCGCC GCTACAGGGC 4861 GCGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA 4921 ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT 4981 TGAAAAAGGA AGAGTCCTGA GGCGGAAAGA ACCAGCTGTG GAATGTGTGT CAGTTAGGGT 5041 GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT 5101 CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC 5161 ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC 5221 CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG 5281 CCGAGGCCGC CTCGGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC 5341 TAGGCTTTTG CAAAGATCGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA 5401 GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG 5461 GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC 5521 CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA AGACGAGGCA 5581 GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC 5641 ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA 5701 TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT 5761 ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA 5821 CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG 5881 CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGAGCA TGCCCGACGG CGAGGATCTC 5941 GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT 6001 GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT ATCAGGACAT AGCGTTGGCT 6061 ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC 6121 GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC 6181 TGAGCGGGAC TCTGGGGTTC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG 6241 ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG 6301 CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCTAGGG 6361 GGAGGCTAAC TGAAACACGG AAGGAGACAA TACCGGAAGG AACCCGCGCT ATGACGGCAA 6421 TAAAAAGACA GAATAAAACG CACGGTGTTG GGTCGTTTGT TCATAAACGC GGGGTTCGGT 6481 CCCAGGGCTG GCACTCTGTC GATACCCCAC CGAGACCCCA TTGGGGCCAA TACGCCCGCG 6541 TTTCTTCCTT TTCCCCACCC CACCCCCCAA GTTCGGGTGA AGGCCCAGGG CTCGCAGCCA 6601 ACGTCGGGGC GGCAGGCCCT GCCATAGCCT CAGGTTACTC ATATATACTT TAGATTGATT 6661 TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA 6721 CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA 6781 AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC 6841 CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG 6901 TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTTCT TCTAGTGTAG CCGTAGTTAG 6961 GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC 7021 CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT 7081 TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG 7141 AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC 7201 TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC 7261 GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC 7321 ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA 7381 ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT 7441 TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCATG CATTAGTTAT 7501 TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT ATATGGAGTT CCG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.