pLV3-CMV-Prdx1-3×HA-CopGFP-Pur

pLV3-CMV-Prdx1-3×HA-CopGFP-Puro plasmid

PVT96108

pLV3-CMV-Prdx1-3×HA-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Prdx1(mouse)-3×HA-CopGFP-Puro
Alias: NM_011034.5;MSP23; NkefA; OSF-3; OSF3; PAG; Paga;PrdxI; prx1; PrxI; Tdpx2; TDX2; TPxA;
Gene lenght: 597bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Prdx1-3×HA-CopGFP-Puro plasmid Description

pLV3-CMV-Prdx1-3×HA-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Prdx1-3×HA-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgt cttcaggaaa tgcaaaaatt 2341 gggtatcctg ctcccaactt caaagctaca gctgttatgc cagatggaca attcaaagat 2401 atcagcctaa gtgaatacaa aggaaaatat gttgtgttct tcttttaccc tcttgacttt 2461 acttttgtgt gtcccacgga gatcattgct ttcagtgata gagccgatga atttaagaaa 2521 ctcaactgcc aagtgattgg cgcttctgtg gattctcact tctgtcatct ggcatggatt 2581 aacacaccca agaaacaagg aggattggga cccatgaaca ttcccttaat atcagatccc 2641 aagcgcacca ttgctcagga ttatggagtc ttaaaggctg atgaaggtat ctctttcagg 2701 ggccttttta ttattgatga taaaggtatc cttcgacaga taacaataaa cgatcttccc 2761 gttggccgct ctgtggatga gattatacga ctagtccagg ccttccagtt cactgacaaa 2821 catggtgaag tgtgtccagc tggctggaaa cctggcagtg ataccatcaa gcctgatgtc 2881 aataagagca aagagtattt ctctaagcag aagCTCGAGT ACCCATACGA CGTACCTGAT 2941 TATGCAACTT ATCCTTATGA TGTACCTGAT TATGCTAGCT ATCCTTATGA TGTCCCAGAC 3001 TACGCATAGg gatccCTGCG ATATCCAGCg cggccgcGaa ggatctgcga tcgctccggt 3061 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc 3121 ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg 3181 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc 3241 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct 3301 cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt 3361 cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt 3421 ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc 3481 agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt 3541 tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga cgccaccatg 3601 gagagcgacg agagcggcct gcccgccatg gagatcgagt gccgcatcac cggcaccctg 3661 aacggcgtgg agttcgagct ggtgggcggc ggagagggca cccccaagca gggccgcatg 3721 accaacaaga tgaagagcac caaaggcgcc ctgaccttca gcccctacct gctgagccac 3781 gtgatgggct acggcttcta ccacttcggc acctacccca gcggctacga gaaccccttc 3841 ctgcacgcca tcaacaacgg cggctacacc aacacccgca tcgagaagta cgaggacggc 3901 ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg gccgcgtgat cggcgacttc 3961 aaggtggtgg gcaccggctt ccccgaggac agcgtgatct tcaccgacaa gatcatccgc 4021 agcaacgcca ccgtggagca cctgcacccc atgggcgata acgtgctggt gggcagcttc 4081 gcccgcacct tcagcctgcg cgacggcggc tactacagct tcgtggtgga cagccacatg 4141 cacttcaaga gcgccatcca ccccagcatc ctgcagaacg ggggccccat gttcgccttc 4201 cgccgcgtgg aggagctgca cagcaacacc gagctgggca tcgtggagta ccagcacgcc 4261 ttcaagaccc ccatcgcctt cgccagatcc cgcgctcagt cgtccaattc tgccgtggac 4321 ggcaccgccg gacccggctc caccggatct cgcgagggca gaggaagtct tctaacatgc 4381 ggtgacgtgg aggagaatcc cggccctatg accgagtaca agcccacggt gcgcctcgcc 4441 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc 4501 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa 4561 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc 4621 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc 4681 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc 4741 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg 4801 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc 4861 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac 4921 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg 4981 tgcatgaccc gcaagcccgg tgcctgaaat caacctctgg attacaaaat ttgtgaaaga 5041 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg 5101 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc 5161 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc 5221 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt 5281 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt 5341 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg 5401 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg 5461 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg 5521 ccggctctgc ggcctcttcc gcgtctccgc cttcgccctc agacgagtcg gatctccctt 5581 tggccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc tgtagatctt 5641 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaaaataa 5701 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc 5761 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa 5821 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag 5881 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa 5941 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt 6001 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct 6061 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc 6121 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 6181 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt 6241 ttggaggcct agacttttgc agagacggcc caaattcgta atcatggtca tagctgtttc 6301 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt 6361 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc 6421 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg 6481 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct 6541 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 6601 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 6661 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 6721 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 6781 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 6841 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 6901 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 6961 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 7021 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 7081 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg 7141 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 7201 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 7261 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 7321 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 7381 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 7441 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 7501 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 7561 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 7621 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 7681 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 7741 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 7801 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 7861 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 7921 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 7981 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 8041 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 8101 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 8161 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 8221 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 8281 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 8341 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 8401 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta 8461 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg 8521 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt 8581 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc 8641 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt 8701 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca 8761 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg 8821 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac 8881 gacgttgtaa aacgacggcc agtgccaagc tg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.