pLV3-CMV-MXD3-3×FLAG-CopGFP-Pu

pLV3-CMV-MXD3-3×FLAG-CopGFP-Puro plasmid

PVT96104

pLV3-CMV-MXD3-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-MXD3(human)-3×FLAG-CopGFP-Puro
Alias: NM_031300.4;BHLHC13; MAD3; MYX
Gene lenght: 618bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-MXD3-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-MXD3-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-MXD3-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtttatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc 2281 tagcgaattc gccaccatgg aacccttggc cagcaacatc caggtcctgc tgcaggcggc 2341 cgagttcctg gagcgccgtg agagagaggc cgagcatggt tatgcgtccc tgtgcccgca 2401 tcgcagtcca ggccccatcc acaggaggaa gaagcgaccc ccccaggctc ctggcgcgca 2461 ggacagcggg cggtcagtgc acaatgaact ggagaagcgc aggagggccc agttgaagcg 2521 gtgcctggag cggctgaagc agcagatgcc cctgggggcc gactgtgccc ggtacaccac 2581 gctgagcctg ctgcgccgtg ccaggatgca catccagaag ctggaggatc aggagcagcg 2641 ggcccgacag ctcaaggaga ggctgcgcag caagcagcag agcctgcagc ggcagctgga 2701 gcagctccgg gggctggcag gggcggccga gcgggagcgg ctgcgggcgg acagtctgga 2761 ctcctcaggc ctctcctctg agcgctcaga ctcagaccaa gaggagctgg aggtggatgt 2821 ggagagcctg gtgtttgggg gtgaggccga gctgctgcgg ggcttcgtcg ccggccagga 2881 gcacagctac tcgcacggcg gcggcgcctg gctaggatcc GACTACAAAG ACCATGACGG 2941 TGATTATAAA GATCATGACA TCGACTACAA GGATGACGAT GACAAGTGAg cggccgcGaa 3001 ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg 3061 agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa 3121 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt 3181 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac 3241 agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc 3301 gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg 3361 cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc 3421 cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac 3481 tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc 3541 ctacgctaga cgccaccatg gagagcgacg agagcggcct gcccgccatg gagatcgagt 3601 gccgcatcac cggcaccctg aacggcgtgg agttcgagct ggtgggcggc ggagagggca 3661 cccccaagca gggccgcatg accaacaaga tgaagagcac caaaggcgcc ctgaccttca 3721 gcccctacct gctgagccac gtgatgggct acggcttcta ccacttcggc acctacccca 3781 gcggctacga gaaccccttc ctgcacgcca tcaacaacgg cggctacacc aacacccgca 3841 tcgagaagta cgaggacggc ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg 3901 gccgcgtgat cggcgacttc aaggtggtgg gcaccggctt ccccgaggac agcgtgatct 3961 tcaccgacaa gatcatccgc agcaacgcca ccgtggagca cctgcacccc atgggcgata 4021 acgtgctggt gggcagcttc gcccgcacct tcagcctgcg cgacggcggc tactacagct 4081 tcgtggtgga cagccacatg cacttcaaga gcgccatcca ccccagcatc ctgcagaacg 4141 ggggccccat gttcgccttc cgccgcgtgg aggagctgca cagcaacacc gagctgggca 4201 tcgtggagta ccagcacgcc ttcaagaccc ccatcgcctt cgccagatcc cgcgctcagt 4261 cgtccaattc tgccgtggac ggcaccgccg gacccggctc caccggatct cgcgagggca 4321 gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctatg accgagtaca 4381 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg 4441 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc 4501 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt 4561 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg 4621 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg 4681 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc 4741 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc 4801 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc 4861 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc 4921 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaaat caacctctgg 4981 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat 5041 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt 5101 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca 5161 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg 5221 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg 5281 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca 5341 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca 5401 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc 5461 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtctccgc cttcgccctc 5521 agacgagtcg gatctccctt tggccgcctc cccgcctggt acctttaaga ccaatgactt 5581 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa 5641 ttcactccca acgaaaataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc 5701 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa 5761 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga 5821 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc 5881 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt 5941 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 6001 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg 6061 tctggctcta gctatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 6121 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca 6181 gaagtagtga ggaggctttt ttggaggcct agacttttgc agagacggcc caaattcgta 6241 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 6301 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt 6361 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta 6421 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 6481 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 6541 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 6601 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 6661 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 6721 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 6781 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 6841 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 6901 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 6961 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 7021 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 7081 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 7141 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 7201 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 7261 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 7321 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 7381 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 7441 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 7501 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 7561 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 7621 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 7681 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 7741 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 7801 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 7861 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 7921 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 7981 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 8041 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 8101 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 8161 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 8221 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 8281 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 8341 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 8401 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc 8461 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga 8521 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc 8581 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact 8641 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat 8701 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc 8761 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac 8821 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.