pLV2-CMV-Gsdmc-EGFP-Puro plasm

pLV2-CMV-Gsdmc-EGFP-Puro plasmid

PVT95494

pLV2-CMV-Gsdmc-EGFP-Puro plasmid Information

Plasmid name: pLV2-CMV-Gsdmc(mouse)-EGFP-Puro
Alias: NM_031378.3;Gsdmc1; Mlze
Gene lenght: 1404bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-Gsdmc-EGFP-Puro plasmid Description

pLV2-CMV-Gsdmc-EGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV2-CMV-Gsdmc-EGFP-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgaG 2821 CCACCatgtc ctacacattt gactggctta gcaaggatgt ggttaaaaag cttcaaggaa 2881 gagatctgag gcctgtcaaa tgcctgtcag atgcaaccaa attctgcctg tttaacatac 2941 ttcaggaaac ttcttcccgg ttggctttga aaactgaata tattccagtt ggatttaccc 3001 ttctgcatct cttggagccc aatattcctg ttccagagcc tgaagtgtca gctcccatcc 3061 cccttaaaca caccatatcc cagaaactga aggctgacct ggatgtcgaa accattgcag 3121 gaggagaagc agggtttgtt aagtcctgtg gatatgacat cgaggttcag agtaagagca 3181 tcccaaatcc aaaactggaa agtcttcaaa acagaaaact gttggatcag ttgcccacat 3241 ttatgaagac ttgctggaag gatggaaaga acctgtatgt ggtgacagag gcctatgaag 3301 tgaccaagga tactgtgctg gaaggcacaa gcaatagcaa atttgcaatc aaagggatca 3361 tcaaccagct tgtcaaggtt ggaggcagtg gacagtggca aacagagaaa acagattcaa 3421 tacctatcca aaagggttca gtgttggcct acaagaagca gcaattggtc attgaggata 3481 acacttgtgt tattctcact tctgctaaca ctaagaagaa gatgaccttt ccaatgagat 3541 ttgtgggaat gtctggacat ctaagatatc aggatctagt cattgagaca ggctcgtgga 3601 taaatgacat cgatccaatt ggaacaatta aggagcccac ccatctagat ttcatgtgcc 3661 tacaaaatga agtttctgaa caaacacggc tactggctga actctcaaag gatgttcaag 3721 aagttgtgtt ctccagtttc ctgcacatgc tctgtgacag ggatgttctg tatgacctga 3781 tgaaaatgct ggagttgaat cagttggggc atatggatgg cccaggtggc aaaatcctgg 3841 atgagcttcg aaaggactcg agcctttcat ggattaacct aaaggacctc attctttatc 3901 tccttcaagc cctgatggtg ctgagtgata cccaactctg tctgctggcc ctgtctgtgg 3961 agatgcggct cctcccgcac caagtggagc tggtaaagag catcttacag ccaaacttca 4021 agtatccctg gaacattccc ttcacactcc agcctcagct tcttgcccca ctccagggtg 4081 agggcttggc cattacttat gaattgctgg aggagtgtgg tctgaagatg gagctgaata 4141 atcccaggtc aacttgggat ttggaagcca agatgcccct gtctgctctc tatggaagcc 4201 tgtcattttt acagcaactt tcagaagccg gtggaggcgg atcaggaggc ggtggatcgg 4261 gcccgatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc 4321 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca 4381 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc 4441 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca 4501 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca 4561 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca 4621 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg 4681 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga 4741 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc 4801 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca 4861 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca 4921 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca 4981 agTAGggatc ccgcgactct agataattct accgggtagg ggaggcgctt ttcccaaggc 5041 agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct 5101 ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc 5161 ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg 5221 cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca 5281 gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct 5341 ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg 5401 ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc 5461 aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca 5521 gcccaagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt 5581 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac 5641 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg 5701 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg 5761 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc 5821 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca 5881 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg 5941 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt 6001 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt 6061 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa 6121 gcccggtgcc tgaccgcgtc tggaacaatc aacctctgga ttacaaaatt tgtgaaagat 6181 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc 6241 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct 6301 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca 6361 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt 6421 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg 6481 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga 6541 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt 6601 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc 6661 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt 6721 gggccgcctc cccgcctgga attaattctg cagtcgagac ctagaaaaac atggagcaat 6781 cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga 6841 ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc 6901 agctgtagat cttagccact ttttaaaaga aaagagggga ctggaagggc taattcactc 6961 ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga 7021 ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta 7081 caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag 7141 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg 7201 gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt 7261 caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag 7321 gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc 7381 tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct 7441 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt 7501 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt 7561 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa 7621 agaaatgaat atcagagagt gagaggcctt gacattgcta gcgttttacc gtcgacctct 7681 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc 7741 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga 7801 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 7861 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 7921 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 7981 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 8041 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 8101 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 8161 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 8221 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 8281 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 8341 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 8401 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 8461 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 8521 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca 8581 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 8641 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 8701 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 8761 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 8821 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 8881 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 8941 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 9001 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 9061 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 9121 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 9181 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 9241 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 9301 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 9361 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 9421 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 9481 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 9541 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 9601 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 9661 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 9721 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 9781 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 9841 cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac ttgtttattg cagcttataa 9901 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 9961 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat 10021 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa 10081 ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag 10141 aaattgtatt tgttaaatat gtactacaaa cttagtagtt tttaaagaaa ttgtatttgt 10201 taaatatgta ctacaaactt agtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.