pLV2-EF1a-Vim-3×FLAG-Puro plas

pLV2-EF1a-Vim-3×FLAG-Puro plasmid

PVT94456

pLV2-EF1a-Vim-3×FLAG-Puro plasmid Information

Plasmid name: pLV2-EF1a-Vim(mouse)-3×FLAG-Puro
Alias: NM_011701.4
Gene lenght: 1396bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: EF1a
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: EF1a-R-F
Reverse primer:
Copy number: High
Induction:

pLV2-EF1a-Vim-3×FLAG-Puro plasmid Description

pLV2-EF1a-Vim-3×FLAG-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV2-EF1a-Vim-3×FLAG-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg 2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc 2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct 2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag 2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg 2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag 2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt 2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt 2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg 2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg 2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct 2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg 2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc 2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga 3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct 3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca 3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg 3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg 3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat 3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg 3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa 3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca 3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaATTAGCTT GGTACgaatt 3541 cGCCACCatg tctaccaggt ctgtgtcctc gtcctcctac cgcaggatgt tcggtggctc 3601 cggcacatcg agccggccca gctccaaccg gagctatgtg accacgtcca cacgcaccta 3661 cagtctgggc agcgcactgc gccccagcac tagccgcagc ctctattcct catcccccgg 3721 tggcgcctat gtgacccggt cctcggcagt gcgcctgcgg agcagcgtgc cgggcgtgcg 3781 gctgcttcaa gactcggtgg acttctcgct ggccgacgcc atcaacactg agttcaagaa 3841 cacccgcacc aacgagaagg tagaactgca ggagctgaat gaccgctttg ccaactacat 3901 cgacaaggtg cgcttcctcg agcagcagaa caaaatcctg ctggctgagc tcgagcagct 3961 caagggccag ggcaagtcgc gcctgggcga cctgtacgag gaggagatgc gggagctgcg 4021 ccggcaggtg gatcagctca ccaacgacaa ggcccgtgtc gaggtggagc gggacaacct 4081 ggccgaggac atcatgcggc tgcgagagaa attgcaggag gagatgctcc agagagagga 4141 agccgaaagc accctgcagt cattcagaca ggatgttgac aatgcttctc tggcacgtct 4201 tgaccttgaa cggaaagtgg aatccttgca ggaagaaatt gcctttttga agaaactgca 4261 cgatgaagag atccaggagc tgcaggccca gattcaggaa cagcatgtcc agatcgatgt 4321 ggacgtttcc aagcctgacc tcactgctgc cctgcgtgat gtgcgccagc agtatgaaag 4381 cgtggctgcc aagaacctcc aggaggccga ggaatggtac aagtccaagt ttgctgacct 4441 ctctgaggct gccaaccgga acaacgatgc cctgcgccag gccaagcagg agtcaaacga 4501 gtaccggaga caggtgcagt cactcacctg tgaagtggat gcccttaaag gcactaacga 4561 gtccctggag cgccagatgc gtgagatgga agagaatttt gcccttgaag ctgctaacta 4621 ccaggacact attggccgcc tgcaggatga gatccaaaac atgaaggaag agatggctcg 4681 tcaccttcgt gaataccaag atctgctcaa tgttaagatg gccctggaca ttgagatcgc 4741 cacctacagg aagctgctgg aaggcgagga gagcaggatt tctctgcctc tgccaacctt 4801 ttcttccctg aacctgagag aaactaacct ggagtcactt cctctggttg acacccactc 4861 aaaaagaaca ctcctgatta agacggttga gaccagagat ggacaggtga tcaatgagac 4921 ttctcagcat cacgatgacc ttgGcggccg cGACTACAAA GACCATGACG GTGATTATAA 4981 AGATCATGAC ATCGACTACA AGGATGACGA TGACAAGTAG TCTAGAcgcc cctctccctc 5041 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta 5101 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc 5161 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct 5221 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt 5281 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa 5341 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg 5401 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga 5461 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac 5521 atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt 5581 cctttgaaaa acacgatgat aagcttgcca caacccacaa ggagacgacc ttccatgacc 5641 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc cgtacgcacc 5701 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc ggaccgccac 5761 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc 5821 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc 5881 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg 5941 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg 6001 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc 6061 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc 6121 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc 6181 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctagacgcgt 6241 ctggaacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta 6301 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc 6361 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga 6421 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac 6481 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc 6541 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc 6601 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg 6661 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc 6721 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc 6781 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg 6841 aattaattct gcagtcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca 6901 gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca 6961 gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac 7021 tttttaaaag aaaagagggg actggaaggg ctaattcact cccaacgaag acaagatatc 7081 cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca 7141 gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag 7201 ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc 7261 ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta 7321 gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga 7381 gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact 7441 ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc 7501 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 7561 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 7621 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag 7681 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag 7741 tgagaggcct tgacattgct agcgtttacc gtcgacctct agctagagct tggcgtaatc 7801 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg 7861 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat 7921 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg 7981 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct 8041 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 8101 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 8161 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 8221 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 8281 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 8341 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 8401 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 8461 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 8521 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 8581 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 8641 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 8701 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 8761 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 8821 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 8881 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 8941 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 9001 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 9061 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 9121 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 9181 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 9241 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 9301 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 9361 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 9421 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 9481 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 9541 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 9601 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 9661 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 9721 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 9781 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 9841 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 9901 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt 9961 cgacggatcg ggagatcaac ttgtttattg cagcttataa tggttacaaa taaagcaata 10021 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca 10081 aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc 10141 atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac 10201 tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat 10261 gtactacaaa cttagtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.