pLV3-U6-Cd200-shRNA2-CopGFP-Hy

pLV3-U6-Cd200-shRNA2-CopGFP-Hyg plasmid

PVT96005

pLV3-U6-Cd200-shRNA2-CopGFP-Hyg plasmid Information

Plasmid name: pLV3-U6-Cd200(mouse)-shRNA2-CopGFP-Hyg
Alias: CAGAGTCTGGACAAAGGATTT
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Interference silence
Fragment type: shRNA
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Hyg
Fluorescent labeling: Green
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pLV3-U6-Cd200-shRNA2-CopGFP-Hyg plasmid Description

pLV3-U6-Cd200-shRNA2-CopGFP-Hyg is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-U6-Cd200-shRNA2-CopGFP-Hyg plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtGAGGGCCT ATTTCCCATG ATTCCTTCAT ATTTGCATAT ACGATACAAG 1981 GCTGTTAGAG AGATAATTAG AATTAATTTG ACTGTAAACA CAAAGATATT AGTACAAAAT 2041 ACGTGACGTA GAAAGTAATA ATTTCTTGGG TAGTTTGCAG TTTTAAAATT ATGTTTTAAA 2101 ATGGACTATC ATATGCTTAC CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC 2161 TTGTGGAAAG GACTCATCgc tagcgCAGAG TCTGGACAAA GGATTTctcg agAAATCCTT 2221 TGTCCAGACT CTGttttttg gatccgcggc cgcGaaggat ctgcgatcgc tccggtgccc 2281 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca 2341 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact 2401 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga 2461 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca 2521 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg 2581 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa 2641 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc 2701 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg 2761 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac gctagacgcc accatggaga 2821 gcgacgagag cggcctgccc gccatggaga tcgagtgccg catcaccggc accctgaacg 2881 gcgtggagtt cgagctggtg ggcggcggag agggcacccc caagcagggc cgcatgacca 2941 acaagatgaa gagcaccaaa ggcgccctga ccttcagccc ctacctgctg agccacgtga 3001 tgggctacgg cttctaccac ttcggcacct accccagcgg ctacgagaac cccttcctgc 3061 acgccatcaa caacggcggc tacaccaaca cccgcatcga gaagtacgag gacggcggcg 3121 tgctgcacgt gagcttcagc taccgctacg aggccggccg cgtgatcggc gacttcaagg 3181 tggtgggcac cggcttcccc gaggacagcg tgatcttcac cgacaagatc atccgcagca 3241 acgccaccgt ggagcacctg caccccatgg gcgataacgt gctggtgggc agcttcgccc 3301 gcaccttcag cctgcgcgac ggcggctact acagcttcgt ggtggacagc cacatgcact 3361 tcaagagcgc catccacccc agcatcctgc agaacggggg ccccatgttc gccttccgcc 3421 gcgtggagga gctgcacagc aacaccgagc tgggcatcgt ggagtaccag cacgccttca 3481 agacccccat cgccttcgcc agatcccgcg ctcagtcgtc caattctgcc gtggacggca 3541 ccgccggacc cggctccacc ggatctcgcg agggcagagg aagtcttcta acatgcggtg 3601 acgtggagga gaatcccggc cctatgaaaa agcctgaact caccgcgacg tctgtcgaga 3661 agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag 3721 aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct 3781 gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc 3841 cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc 3901 gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc 3961 agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt 4021 tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg 4081 cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt 4141 ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc 4201 acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag 4261 cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct 4321 tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc 4381 atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc 4441 aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat 4501 gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa 4561 gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc 4621 ccagcactcg tccgagggca aaggaatagg tcgacaatca acctctggat tacaaaattt 4681 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg 4741 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt 4801 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg 4861 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc 4921 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg 4981 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt 5041 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc 5101 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg 5161 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga 5221 tctccctttg ggccgcctcc ccgcggtacc tttaagacca atgacttaca aggcagctgt 5281 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg 5341 aaaataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg 5401 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt 5461 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc 5521 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta 5581 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta 5641 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 5701 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct 5761 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 5821 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga 5881 ggcttttttg gaggcctaga cttttgcaga gacggcccaa attcgtaatc atggtcatag 5941 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc 6001 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc 6061 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa 6121 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg 6181 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 6241 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 6301 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 6361 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 6421 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 6481 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 6541 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 6601 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 6661 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 6721 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 6781 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 6841 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 6901 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 6961 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 7021 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 7081 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 7141 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 7201 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 7261 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 7321 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 7381 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt 7441 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 7501 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 7561 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 7621 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 7681 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga 7741 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 7801 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 7861 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 7921 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 7981 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 8041 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc 8101 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg 8161 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct 8221 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc 8281 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat 8341 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg 8401 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc 8461 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc 8521 cagtcacgac gttgtaaaac gacggccagt gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.