pLV3-CMV-ADRB1-3×FLAG-CopGFP-P

pLV3-CMV-ADRB1-3×FLAG-CopGFP-Puro plasmid

PVT95951

pLV3-CMV-ADRB1-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-ADRB1(human-gc-opt)-3×FLAG-CopGFP-Puro
Alias: NM_000684.3;ADRB1R; B1AR; BETA1AR; FNSS2; RHR
Gene lenght: 1431bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-ADRB1-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-ADRB1-3×FLAG-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-ADRB1-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccATGG GAGCTGGAGT GCTGGTGCTG 2341 GGAGCTTCTG AACCTGGAAA CCTGAGCAGC GCTGCCCCTC TGCCTGATGG AGCTGCTACA 2401 GCTGCTAGGC TGCTGGTGCC TGCTTCTCCT CCTGCTAGTC TGCTGCCTCC TGCTTCTGAG 2461 TCCCCTGAAC CTCTGAGCCA GCAGTGGACA GCTGGAATGG GACTGCTGAT GGCCCTGATT 2521 GTGCTGCTGA TCGTGGCCGG AAACGTGCTG GTGATCGTGG CTATTGCCAA GACCCCCAGA 2581 CTGCAGACCC TGACAAACCT GTTTATCATG AGTCTGGCCT CTGCCGACCT GGTGATGGGA 2641 CTGCTGGTGG TGCCTTTTGG AGCTACCATT GTGGTGTGGG GCAGATGGGA ATATGGAAGC 2701 TTCTTCTGCG AGCTGTGGAC CAGCGTGGAC GTGCTGTGTG TGACTGCTTC TATCGAGACC 2761 CTGTGTGTGA TCGCCCTGGA CAGATACCTG GCTATTACCA GCCCCTTCCG GTATCAGTCT 2821 CTGCTGACCA GAGCTAGAGC CAGAGGACTG GTGTGTACCG TGTGGGCTAT CAGCGCTCTG 2881 GTGTCTTTCC TGCCCATTCT GATGCACTGG TGGAGAGCCG AGAGCGATGA GGCTAGAAGA 2941 TGCTACAACG ACCCCAAATG CTGCGACTTC GTGACCAATA GAGCCTACGC CATCGCCTCC 3001 AGCGTGGTGT CTTTCTACGT GCCTCTGTGT ATCATGGCCT TCGTGTATCT GAGAGTGTTT 3061 AGGGAAGCCC AGAAGCAGGT GAAAAAGATC GACAGCTGCG AACGCAGATT CCTGGGCGGA 3121 CCTGCTAGAC CTCCTTCTCC TTCTCCCAGC CCTGTGCCTG CTCCTGCTCC TCCTCCTGGA 3181 CCTCCTAGAC CTGCTGCTGC TGCTGCCACA GCTCCTCTGG CTAATGGAAG AGCCGGCAAA 3241 CGGAGGCCTT CCAGACTGGT GGCTCTGAGA GAGCAGAAAG CCCTGAAAAC CCTGGGCATC 3301 ATCATGGGCG TGTTCACCCT GTGCTGGCTG CCTTTTTTTC TGGCCAACGT GGTGAAGGCC 3361 TTCCATAGAG AGCTGGTGCC CGACAGACTG TTCGTGTTCT TTAACTGGCT GGGGTACGCC 3421 AACTCTGCCT TCAACCCTAT CATCTACTGC AGGTCTCCCG ACTTCCGGAA GGCTTTCCAG 3481 GGACTGCTGT GCTGTGCCAG AAGGGCTGCT AGAAGGAGAC ACGCTACCCA CGGAGACAGA 3541 CCAAGAGCTA GCGGATGCCT GGCTAGACCA GGACCTCCTC CTTCTCCAGG AGCTGCTTCT 3601 GACGATGACG ACGACGACGT GGTGGGAGCT ACACCTCCTG CTAGACTGCT GGAGCCATGG 3661 GCTGGATGTA ACGGAGGAGC TGCTGCTGAT TCCGACAGCT CTCTGGATGA ACCCTGCAGA 3721 CCCGGATTTG CCTCCGAAAG TAAGGTGgga tccGACTACA AAGACCATGA CGGTGATTAT 3781 AAAGATCATG ACATCGACTA CAAGGATGAC GATGACAAGt agcggccgcG aaggatctgc 3841 gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg 3901 gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa 3961 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt 4021 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag 4081 cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca 4141 cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc 4201 gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc 4261 ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc 4321 tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctacgcta 4381 gacgccacca tggagagcga cgagagcggc ctgcccgcca tggagatcga gtgccgcatc 4441 accggcaccc tgaacggcgt ggagttcgag ctggtgggcg gcggagaggg cacccccaag 4501 cagggccgca tgaccaacaa gatgaagagc accaaaggcg ccctgacctt cagcccctac 4561 ctgctgagcc acgtgatggg ctacggcttc taccacttcg gcacctaccc cagcggctac 4621 gagaacccct tcctgcacgc catcaacaac ggcggctaca ccaacacccg catcgagaag 4681 tacgaggacg gcggcgtgct gcacgtgagc ttcagctacc gctacgaggc cggccgcgtg 4741 atcggcgact tcaaggtggt gggcaccggc ttccccgagg acagcgtgat cttcaccgac 4801 aagatcatcc gcagcaacgc caccgtggag cacctgcacc ccatgggcga taacgtgctg 4861 gtgggcagct tcgcccgcac cttcagcctg cgcgacggcg gctactacag cttcgtggtg 4921 gacagccaca tgcacttcaa gagcgccatc caccccagca tcctgcagaa cgggggcccc 4981 atgttcgcct tccgccgcgt ggaggagctg cacagcaaca ccgagctggg catcgtggag 5041 taccagcacg ccttcaagac ccccatcgcc ttcgccagat cccgcgctca gtcgtccaat 5101 tctgccgtgg acggcaccgc cggacccggc tccaccggat ctcgcgaggg cagaggaagt 5161 cttctaacat gcggtgacgt ggaggagaat cccggcccta tgaccgagta caagcccacg 5221 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc 5281 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc 5341 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg 5401 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg 5461 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa 5521 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc 5581 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga 5641 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac 5701 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga 5761 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa atcaacctct ggattacaaa 5821 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac 5881 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc 5941 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt 6001 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc 6061 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc 6121 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg 6181 gtgttgtcgg ggaaATCATC GTCCTTTCCT tggctgctcg cctgtgttgc cacctggatt 6241 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc 6301 cgcggcctgc tgccggctct gcggcctctt ccgcgtctTc gccttcgccc tcagacgagt 6361 cggatctccc tttggGccgc ctccccgcct ggtaccttta agaccaatga cttacaaggc 6421 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc 6481 ccaacgaaGa taagatctgc tttttgcttg tactgggtct ctctggttag accagatctg 6541 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc 6601 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct 6661 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat 6721 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc 6781 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 6841 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct 6901 ctagctaTCC CGCCCCTAAC TCCGCCCAtc ccgcccctaa ctccgcccag ttccgcccat 6961 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc 7021 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagactt ttgcagagac 7081 caaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc 7141 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta 7201 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca 7261 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc 7321 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 7381 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 7441 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 7501 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 7561 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 7621 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 7681 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 7741 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 7801 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 7861 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 7921 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt 7981 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 8041 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 8101 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 8161 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 8221 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc 8281 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta 8341 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga 8401 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg 8461 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc 8521 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat 8581 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag 8641 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat 8701 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa 8761 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa 8821 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga 8881 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg 8941 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc 9001 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg 9061 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact 9121 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat 9181 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 9241 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat 9301 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca 9361 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca 9421 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca 9481 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa 9541 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt 9601 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag 9661 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct 9721 g

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.