pLV3-CMV-HRAS-G12V-large T-Bla

pLV3-CMV-HRAS-G12V-large T-Blast plasmid

PVT95804

pLV3-CMV-HRAS-G12V-large T-Blast plasmid Information

Plasmid name: pLV3-CMV-HRAS(human)-G12V-large T-Blast
Alias: NM_005343.4;C-BAS/HAS; C-H-RAS; C-HA-RAS1; CTLO;H-RASIDX; HAMSV; HRAS1; p21ras; RASH1
Gene lenght: 567bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Blast
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): BL21(DE3)
Growth Temperature: 30°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: Low
Induction:

pLV3-CMV-HRAS-G12V-large T-Blast plasmid Description

pLV3-CMV-HRAS-G12V-large T-Blast is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-HRAS-G12V-large T-Blast plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 1981 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 2041 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 2101 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 2161 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG 2221 TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT 2281 AGAGCTAGCG AATTCgccac cATGACCGAA TACAAGCTTG TTGTTGTTGG CGCCGTCGGT 2341 GTGGGCAAGA GTGCGCTGAC CATCCAGCTG ATCCAGAACC ATTTTGTGGA CGAATACGAC 2401 CCCACTATAG AGGATTCCTA CCGGAAGCAG GTGGTCATTG ATGGGGAGAC GTGCCTGTTG 2461 GACATCCTGG ATACCGCCGG CCAGGAGGAG TACAGCGCCA TGCGGGACCA GTACATGCGC 2521 ACCGGGGAGG GCTTCCTGTG TGTGTTTGCC ATCAACAACA CCAAGTCTTT TGAGGACATC 2581 CACCAGTACA GGGAGCAGAT CAAACGGGTG AAGGACTCGG ATGACGTGCC CATGGTGCTG 2641 GTGGGGAACA AGTGTGACCT GGCTGCACGC ACTGTGGAAT CTCGGCAGGC TCAGGACCTC 2701 GCCCGAAGCT ACGGCATCCC CTACATCGAG ACCTCGGCCA AGACCCGGCA GGGAGTGGAG 2761 GATGCCTTCT ACACGTTGGT GCGTGAGATC CGGCAGCACA AGCTGCGGAA GCTGAACCCT 2821 CCTGATGAGA GTGGCCCCGG CTGCATGAGC TGCAAGTGTG TGCTCTCCgg tggaggcgga 2881 tcaggaggcg gtggatctAT GGATAAAGTT TTAAACAGAG AGGAATCTTT GCAGCTAATG 2941 GACCTTCTAG GTCTTGAAAG GAGTGCCTGG GGGAATATTC CTCTGATGAG AAAGGCATAT 3001 TTAAAAAAAT GCAAGGAGTT TCATCCTGAT AAAGGAGGAG ATGAAGAAAA AATGAAGAAA 3061 ATGAATACTC TGTACAAGAA AATGGAAGAT GGAGTAAAAT ATGCTCATCA ACCTGACTTT 3121 GGAGGCTTCT GGGATGCAAC TGAGATTCCA ACCTATGGAA CTGATGAATG GGAGCAGTGG 3181 TGGAATGCCT TTAATGAGGA AAACCTGTTT TGCTCAGAAG AAATGCCATC TAGTGATGAT 3241 GAGGCTACTG CTGACTCTCA ACATTCTACT CCTCCAAAAA AGAAGAGAAA GGTAGAAGAC 3301 CCCAAGGACT TTCCTTCAGA ATTGCTAAGT TTTTTGAGTC ATGCTGTGTT TAGTAATAGA 3361 ACTCTTGCTT GCTTTGCTAT TTACACCACA AAGGAAAAAG CTGCACTGCT ATACAAGAAA 3421 ATTATGGAAA AATATTCTGT AACCTTTATA AGTAGGCATA ACAGTTATAA TCATAACATA 3481 CTGTTTTTTC TTACTCCACA CAGGCATAGA GTGTCTGCTA TTAATAACTA TGCTCAAAAA 3541 TTGTGTACCT TTAGCTTTTT AATTTGTAAA GGGGTTAATA AGGAATATTT GATGTATAGT 3601 GCCTTGACTA GAGATCCATT TTCTGTTATT GAGGAAAGTT TGCCAGGTGG GTTAAAGGAG 3661 CATGATTTTA ATCCAGAAGA AGCAGAGGAA ACTAAACAAG TGTCCTGGAA GCTTGTAACA 3721 GAGTATGCAA TGGAAACAAA ATGTGATGAT GTGTTGTTAT TGCTTGGGAT GTACTTGGAA 3781 TTTCAGTACA GTTTTGAAAT GTGTTTAAAA TGTATTAAAA AAGAACAGCC CAGCCACTAT 3841 AAGTACCATG AAAAGCATTA TGCAAATGCT GCTATATTTG CTGACAGCAA AAACCAAAAA 3901 ACCATATGCC AACAGGCTGT TGATACTGTT TTAGCTAAAA AGCGGGTTGA TAGCCTACAA 3961 TTAACTAGAG AACAAATGTT AACAAACAGA TTTAATGATC TTTTGGATAG GATGGATATA 4021 ATGTTTGGTT CTACAGGCTC TGCTGACATA GAAGAATGGA TGGCTGGAGT TGCTTGGCTA 4081 CACTGTTTGT TGCCCAAAAT GGATTCAGTG GTGTATGACT TTTTAAAATG CATGGTGTAC 4141 AACATTCCTA AAAAAAGATA CTGGCTGTTT AAAGGACCAA TTGATAGTGG TAAAACTACA 4201 TTAGCAGCTG CTTTGCTTGA ATTATGTGGG GGGAAAGCTT TAAATGTTAA TTTGCCCTTG 4261 GACAGGCTGA ACTTTGAGCT AGGAGTAGCT ATTGACCAGT TTTTAGTAGT TTTTGAGGAT 4321 GTAAAGGGCA CTGGAGGGGA GTCCAGAGAT TTGCCTTCAG GTCAGGGAAT TAATAACCTG 4381 GACAATTTAA GGGATTATTT GGATGGCAGT GTTAAGGTAA ACTTAGAAAA GAAACACCTA 4441 AATAAAAGAA CTCAAATATT TCCCCCTGGA ATAGTCACCA TGAATGAGTA CAGTGTGCCT 4501 AAAACACTGC AGGCCAGATT TGTAAAACAA ATAGATTTTA GGCCCAAAGA TTATTTAAAG 4561 CATTGCCTGG AACGCAGTGA GTTTTTGTTA GAAAAGAGAA TAATTCAAAG TGGCATTGCT 4621 TTGCTTCTTA TGTTAATTTG GTACAGACCT GTGGCTGAGT TTGCTCAAAG TATTCAGAGC 4681 AGAATTGTGG AGTGGAAAGA GAGATTGGAC AAAGAGTTTA GTTTGTCAGT GTATCAAAAA 4741 ATGAAGTTTA ATGTGGCTAT GGGAATTGGA GTTTTAGATT GGCTAAGAAA CAGTGATGAT 4801 GATGATGAAG ACAGCCAGGA AAATGCTGAT AAAAATGAAG ATGGTGGGGA GAAGAACATG 4861 GAAGACTCAG GGCATGAAAC AGGCATTGAT TCACAGTCCC AAGGCTCATT TCAGGCCCCT 4921 CAGTCCTCAC AGTCTGTTCA TGATCATAAT CAGCCATACC ACATTTGTAG AGGTTTTACT 4981 TGCTTTAAAA AACCTCCCAC ACCTCCCCCT GAACCTGAAA CATAAGGATC CGCGGCCGCG 5041 AAGGATCTGC GATCGCTCCG GTGCCCGTCA GTGGGCAGAG CGCACATCGC CCACAGTCCC 5101 CGAGAAGTTG GGGGGAGGGG TCGGCAATTG AACGGGTGCC TAGAGAAGGT GGCGCGGGGT 5161 AAACTGGGAA AGTGATGTCG TGTACTGGCT CCGCCTTTTT CCCGAGGGTG GGGGAGAACC 5221 GTATATAAGT GCAGTAGTCG CCGTGAACGT TCTTTTTCGC AACGGGTTTG CCGCCAGAAC 5281 ACAGCTGAAG CTTCGAGGGG CTCGCATCTC TCCTTCACGC GCCCGCCGCC CTACCTGAGG 5341 CCGCCATCCA CGCCGGTTGA GTCGCGTTCT GCCGCCTCCC GCCTGTGGTG CCTCCTGAAC 5401 TGCGTCCGCC GTCTAGGTAA GTTTAAAGCT CAGGTCGAGA CCGGGCCTTT GTCCGGCGCT 5461 CCCTTGGAGC CTACCTAGAC TCAGCCGGCT CTCCACGCTT TGCCTGACCC TGCTTGCTCA 5521 ACTCTACGTC TTTGTTTCGT TTTCTGTTCT GCGCCGTTAC AGATCCAAGC TGTGACCGGC 5581 GCCTACGCTA GGCCACCATG GCCAAGCCTT TGTCTCAAGA AGAATCCACC CTCATTGAAA 5641 GAGCAACGGC TACAATCAAC AGCATCCCCA TCTCTGAAGA CTACAGCGTC GCCAGCGCAG 5701 CTCTCTCTAG CGACGGCCGC ATCTTCACTG GTGTCAATGT ATATCATTTT ACTGGGGGAC 5761 CTTGTGCAGA ACTCGTGGTG CTGGGCACTG CTGCTGCTGC GGCAGCTGGC AACCTGACTT 5821 GTATCGTCGC GATCGGAAAT GAGAACAGGG GCATCTTGAG CCCCTGCGGA CGGTGCCGAC 5881 AGGTGCTTCT CGATCTGCAT CCTGGGATCA AAGCCATAGT GAAGGACAGT GATGGACAGC 5941 CGACGGCAGT TGGGATTCGT GAATTGCTGC CCTCTGGTTA TGTGTGGGAG GGCTAAGTCG 6001 ACAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC TGGTATTCTT AACTATGTTG 6061 CTCCTTTTAC GCTATGTGGA TACGCTGCTT TAATGCCTTT GTATCATGCT ATTGCTTCCC 6121 GTATGGCTTT CATTTTCTCC TCCTTGTATA AATCCTGGTT GCTGTCTCTT TATGAGGAGT 6181 TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG TGTGCACTGT GTTTGCTGAC GCAACCCCCA 6241 CTGGTTGGGG CATTGCCACC ACCTGTCAGC TCCTTTCCGG GACTTTCGCT TTCCCCCTCC 6301 CTATTGCCAC GGCGGAACTC ATCGCCGCCT GCCTTGCCCG CTGCTGGACA GGGGCTCGGC 6361 TGTTGGGCAC TGACAATTCC GTGGTGTTGT CGGGGAAATC ATCGTCCTTT CCTTGGCTGC 6421 TCGCCTGTGT TGCCACCTGG ATTCTGCGCG GGACGTCCTT CTGCTACGTC CCTTCGGCCC 6481 TCAATCCAGC GGACCTTCCT TCCCGCGGCC TGCTGCCGGC TCTGCGGCCT CTTCCGCGTC 6541 TTCGCCTTCG CCCTCAGACG AGTCGGATCT CCCTTTGGGC CGCCTCCCCG CCTGGTACCT 6601 TTAAGACCAA TGACTTACAA GGCAGCTGTA GATCTTAGCC ACTTTTTAAA AGAAAAGGGG 6661 GGACTGGAAG GGCTAATTCA CTCCCAACGA AAATAAGATC TGCTTTTTGC TTGTACTGGG 6721 TCTCTCTGGT TAGACCAGAT CTGAGCCTGG GAGCTCTCTG GCTAACTAGG GAACCCACTG 6781 CTTAAGCCTC AATAAAGCTT GCCTTGAGTG CTTCAAGTAG TGTGTGCCCG TCTGTTGTGT 6841 GACTCTGGTA ACTAGAGATC CCTCAGACCC TTTTAGTCAG TGTGGAAAAT CTCTAGCAGT 6901 AGTAGTTCAT GTCATCTTAT TATTCAGTAT TTATAACTTG CAAAGAAATG AATATCAGAG 6961 AGTGAGAGGA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA 7021 AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC 7081 AATGTATCTT ATCATGTCTG GCTCTAGCTA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC 7141 ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG 7201 CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGAC TTTTGCAGAG 7261 ACGGCCCAAA TTCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA 7321 CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG 7381 TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT 7441 CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC 7501 GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG 7561 TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA 7621 AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG 7681 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA 7741 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG 7801 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG 7861 GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC 7921 GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG 7981 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA 8041 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT 8101 GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG 8161 TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG 8221 GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC 8281 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT 8341 TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT 8401 TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA 8461 GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG 8521 TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC 8581 CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG 8641 CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC 8701 GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA 8761 CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC 8821 GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC 8881 CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC 8941 TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT 9001 CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA 9061 TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT 9121 CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA 9181 CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA 9241 AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC 9301 TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG 9361 GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC 9421 GAAAAGTGCC ACCTGACGTC TAAGAAACCA TTATTATCAT GACATTAACC TATAAAAATA 9481 GGCGTATCAC GAGGCCCTTT CGTCTCGCGC GTTTCGGTGA TGACGGTGAA AACCTCTGAC 9541 ACATGCAGCT CCCGGAGACG GTCACAGCTT GTCTGTAAGC GGATGCCGGG AGCAGACAAG 9601 CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG CTGGCTTAAC TATGCGGCAT 9661 CAGAGCAGAT TGTACTGAGA GTGCACCATA TGCGGTGTGA AATACCGCAC AGATGCGTAA 9721 GGAGAAAATA CCGCATCAGG CGCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC 9781 GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA AGGGGGATGT GCTGCAAGGC 9841 GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTG 9901 CCAAGCTG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.