pLV3-CMV-Shisa5-EGFP-EF1a-Fluc

pLV3-CMV-Shisa5-EGFP-EF1a-Fluc-Puro plasmid

PVT95826

pLV3-CMV-Shisa5-EGFP-EF1a-Fluc-Puro plasmid Information

Plasmid name: pLV3-CMV-Shisa5(mouse)-EGFP-EF1a-Fluc-Puro
Alias: NM_025858.3;2310008D10Rik; 6430628I05Rik; mShisa5;Scotin
Gene lenght: 705bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green,Fluc
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Shisa5-EGFP-EF1a-Fluc-Puro plasmid Description

pLV3-CMV-Shisa5-EGFP-EF1a-Fluc-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-Shisa5-EGFP-EF1a-Fluc-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcGCCAC Catggctgcg ccggcgccct ctctgtggac cctattgctg 2341 ctgctgttgc tgctgccgcc gcctccgggt gcccatggtg agctgtgcag gccctttggt 2401 gaagacaatt cgatcccagt gttctgtcct gatttctgtt gtggttcctg ttccaaccaa 2461 tactgctgct cggacgtgct gaggaaaatc cagtggaatg aggaaatgtg tcctgagcca 2521 gagtccagat tttccacccc cgcggaggag acacccgaac atctgggttc agcgctgaaa 2581 tttcgatcca gttttgacag tgaccctatg tcagggttcg gagcgaccgt cgccattggc 2641 gtgaccatct ttgtggtgtt tattgccact atcatcatct gcttcacctg ctcctgctgc 2701 tgtctgtata agatgtgctg cccccaacgc cctgtcgtga ccaacaccac aactactacc 2761 gtggttcatg ccccttaccc tcagcctcaa cctcaacctg tggcccccag ctatcctgga 2821 ccaacatacc agggctacca tcccatgccc ccccagccag gaatgccagc agcaccctac 2881 ccaacgcagt acccaccacc ctacctggcc cagcccacag ggccgccacc ctaccatgag 2941 tccttggctg gagccagcca gcctccatac aacccgacct acatggattc cctaaagaca 3001 attcccggat ccGGCGGTGG AGGAAGCggc ggaggtggat ctatggtgag caagggcgag 3061 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac 3121 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag 3181 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc 3241 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag 3301 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac 3361 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg 3421 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac 3481 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc 3541 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac 3601 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc 3661 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc 3721 gccgccggga tcactctcgg catggacgag ctgtacaagt agggatccgc ggccgcaagg 3781 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag 3841 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac 3901 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat 3961 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag 4021 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc 4081 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg 4141 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct 4201 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc 4261 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct 4321 acgctagacg ccaccatgga gagcgacgag agcggcctga tggaagacgc caaaaacata 4381 aagaaaggcc cggcgccatt ctatccgctg gaagatggaa ccgctggaga gcaactgcat 4441 aaggctatga agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc 4501 gaggtggaca tcacttacgc tgagtacttc gaaatgtccg ttcggttggc agaagctatg 4561 aaacgatatg ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa 4621 ttctttatgc cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac 4681 atttataatg aacgtgaatt gctcaacagt atgggcattt cgcagcctac cgtggtgttc 4741 gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa 4801 aaaattatta tcatggattc taaaacggat taccagggat ttcagtcgat gtacacgttc 4861 gtcacatctc atctacctcc cggttttaat gaatacgatt ttgtgccaga gtccttcgat 4921 agggacaaga caattgcact gatcatgaac tcctctggat ctactggtct gcctaaaggt 4981 gtcgctctgc ctcatagaac tgcctgcgtg agattctcgc atgccagaga tcctattttt 5041 ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg ttccattcca tcacggtttt 5101 ggaatgttta ctacactcgg atatttgata tgtggatttc gagtcgtctt aatgtataga 5161 tttgaagaag agctgtttct gaggagcctt caggattaca agattcaaag tgcgctgctg 5221 gtgccaaccc tattctcctt cttcgccaaa agcactctga ttgacaaata cgatttatct 5281 aatttacacg aaattgcttc tggtggcgct cccctctcta aggaagtcgg ggaagcggtt 5341 gccaagaggt tccatctgcc aggtatcagg caaggatatg ggctcactga gactacatca 5401 gctattctga ttacacccga gggggatgat aaaccgggcg cggtcggtaa agttgttcca 5461 ttttttgaag cgaaggttgt ggatctggat accgggaaaa cgctgggcgt taatcaaaga 5521 ggcgaactgt gtgtgagagg tcctatgatt atgtccggtt atgtaaacaa tccggaagcg 5581 accaacgcct tgattgacaa ggatggatgg ctacattctg gagacatagc ttactgggac 5641 gaagacgaac acttcttcat cgttgaccgc ctgaagtctc tgattaagta caaaggctat 5701 caggtggctc ccgctgaatt ggaatccatc ttgctccaac accccaacat cttcgacgca 5761 ggtgtcgcag gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg 5821 gagcacggaa agacgatgac ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca 5881 accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa aggtcttacc 5941 ggaaaactcg acgcaagaaa aatcagagag atcctcataa aggccaagaa gggcggaaag 6001 atcgccgtga gatcccgcgc tcagtcgtcc aattctgccg tggacggcac cgccggaccc 6061 ggctccaccg gatctcgcga gggcagagga agtcttctaa catgcggtga cgtggaggag 6121 aatcccggcc ctatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc 6181 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc 6241 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc 6301 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg 6361 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc 6421 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac 6481 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc 6541 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg 6601 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc 6661 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag 6721 cccggtgcct gaaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt 6781 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct 6841 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt 6901 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac 6961 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct 7021 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca 7081 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt 7141 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc 7201 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct 7261 cttccgcgtc tccgccttcg ccctcagacg agtcggatct ccctttggcc gcctccccgc 7321 ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa 7381 gaaaaggggg gactggaagg gctaattcac tcccaacgaa aataagatct gctttttgct 7441 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg 7501 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt 7561 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc 7621 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga 7681 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat 7741 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 7801 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca 7861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg 7921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctagact 7981 tttgcagaga cggcccaaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt 8041 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg 8101 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg 8161 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc 8221 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 8281 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 8341 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 8401 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 8461 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 8521 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 8581 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 8641 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 8701 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 8761 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 8821 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc 8881 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 8941 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 9001 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 9061 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 9121 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 9181 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 9241 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 9301 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 9361 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 9421 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 9481 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 9541 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 9601 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 9661 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 9721 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 9781 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 9841 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 9901 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 9961 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 10021 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 10081 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 10141 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct 10201 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa 10261 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga 10321 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact 10381 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca 10441 gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt 10501 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg 10561 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga 10621 cggccagtgc caagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.