pCMV-TRPM8-3×FLAG plasmid

pCMV-TRPM8-3×FLAG plasmid

PVT90791

pCMV-TRPM8-3×FLAG plasmid Information

Plasmid name: pCMV-TRPM8(human)-3×FLAG
Alias: NM_024080.5;LTrpC-6; LTRPC6; trp-p8; TRPP8
Gene lenght: 3312bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: WPRE-R
Copy number: High
Induction:

pCMV-TRPM8-3×FLAG plasmid Description

pCMV-TRPM8-3×FLAG is a plasmid suitable for protein expression in mammalian cells.

pCMV-TRPM8-3×FLAG plasmid Sequence

1 GGCATTGATT ATTGACTAGT TATTAATAGT AATCAATTAC GGGGTCATTA GTTCATAGCC 61 CATATATGGA GTTCCGCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC TGACCGCCCA 121 ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC CATAGTAACG CCAATAGGGA 181 CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG GCAGTACATC 241 AAGTGTATCA TATGCCAAGT CCGCCCCCTA TTGACGTCAA TGACGGTAAA TGGCCCGCCT 301 GGCATTATGC CCAGTACATG ACCTTACGGG ACTTTCCTAC TTGGCAGTAC ATCTACGTAT 361 TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CACCAATGGG CGTGGATAGC 421 GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG AGTTTGTTTT 481 GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAATAA CCCCGCCCCG TTGACGCAAA 541 TGGGCGGTAG GCGTGTACGG TGGGAGGTCT ATATAAGCAG AGCTCGTTTA GTGAACCGTC 601 AGATCAAGGT AAGTATCAAG GTTACAAGAC AGGTTTAAGG AGACCAATAG AAACTGGGCT 661 TGTCGAGACA GAGAAGACTC TTGCGTTTCT GATAGGCACC TATTGGTCTT ACTGACATCC 721 ACTTTGCCTT TCTCTCCACA GGTGTAAGGA ATTCGCCACC atgtcctttc gggcagccag 781 gctcagcatg aggaacagaa ggaatgacac tctggacagc acccggaccc tgtactccag 841 cgcgtctcgg agcacagact tgtcttacag tgaaagcgac ttggtgaatt ttattcaagc 901 aaattttaag aaacgagaat gtgtcttctt taccaaagat tccaaggcca cggagaatgt 961 gtgcaagtgt ggctatgccc agagccagca catggaaggc acccagatca accaaagtga 1021 gaaatggaac tacaagaaac acaccaagga atttcctacc gacgcctttg gggatattca 1081 gtttgagaca ctggggaaga aagggaagta tatacgtctg tcctgcgaca cggacgcgga 1141 aatcctttac gagctgctga cccagcactg gcacctgaaa acacccaacc tggtcatttc 1201 tgtgaccggg ggcgccaaga acttcgccct gaagccgcgc atgcgcaaga tcttcagccg 1261 gctcatctac atcgcgcagt ccaaaggtgc ttggattctc acgggaggca cccattatgg 1321 cctgatgaag tacatcgggg aggtggtgag agataacacc atcagcagga gttcagagga 1381 gaatattgtg gccattggca tagcagcttg gggcatggtc tccaaccggg acaccctcat 1441 caggaattgc gatgctgagg gctatttttt agcccagtac cttatggatg acttcacaag 1501 agatccactg tatatcctgg acaacaacca cacacatttg ctgctcgtgg acaatggctg 1561 tcatggacat cccactgtcg aagcaaagct ccggaatcag ctagagaagt atatctctga 1621 gcgcactatt caagattcca actatggtgg caagatcccc attgtgtgtt ttgcccaagg 1681 aggtggaaaa gagactttga aagccatcaa tacctccatc aaaaataaaa ttccttgtgt 1741 ggtggtggaa ggctcgggcc agatcgctga tgtgatcgct agcctggtgg aggtggagga 1801 tgccctgaca tcttctgccg tcaaggagaa gctggtgcgc tttttacccc gcacggtgtc 1861 ccggctgcct gaggaggaga ctgagagttg gatcaaatgg ctcaaagaaa ttctcgaatg 1921 ttctcaccta ttaacagtta ttaaaatgga agaagctggg gatgaaattg tgagcaatgc 1981 catctcctac gctctataca aagccttcag caccagtgag caagacaagg ataactggaa 2041 tgggcagctg aagcttctgc tggagtggaa ccagctggac ttagccaatg atgagatttt 2101 caccaatgac cgccgatggg agtctgctga ccttcaagaa gtcatgttta cggctctcat 2161 aaaggacaga cccaagtttg tccgcctctt tctggagaat ggcttgaacc tacggaagtt 2221 tctcacccat gatgtcctca ctgaactctt ctccaaccac ttcagcacgc ttgtgtaccg 2281 gaatctgcag atcgccaaga attcctataa tgatgccctc ctcacgtttg tctggaaact 2341 ggttgcgaac ttccgaagag gcttccggaa ggaagacaga aatggccggg acgagatgga 2401 catagaactc cacgacgtgt ctcctattac tcggcacccc ctgcaagctc tcttcatctg 2461 ggccattctt cagaataaga aggaactctc caaagtcatt tgggagcaga ccaggggctg 2521 cactctggca gccctgggag ccagcaagct tctgaagact ctggccaaag tgaagaacga 2581 catcaatgct gctggggagt ccgaggagct ggctaatgag tacgagaccc gggctgttga 2641 gctgttcact gagtgttaca gcagcgatga agacttggca gaacagctgc tggtctattc 2701 ctgtgaagct tggggtggaa gcaactgtct ggagctggcg gtggaggcca cagaccagca 2761 tttcatcgcc cagcctgggg tccagaattt tctttctaag caatggtatg gagagatttc 2821 ccgagacacc aagaactgga agattatcct gtgtctgttt attataccct tggtgggctg 2881 tggctttgta tcatttagga agaaacctgt cgacaagcac aagaagctgc tttggtacta 2941 tgtggcgttc ttcacctccc ccttcgtggt cttctcctgg aatgtggtct tctacatcgc 3001 cttcctcctg ctgtttgcct acgtgctgct catggatttc cattcggtgc cacacccccc 3061 cgagctggtc ctgtactcgc tggtctttgt cctcttctgt gatgaagtga gacagtggta 3121 cgtaaatggg gtgaattatt ttactgacct gtggaatgtg atggacacgc tggggctttt 3181 ttacttcata gcaggaattg tatttcggct ccactcttct aataaaagct ctttgtattc 3241 tggacgagtc attttctgtc tggactacat tattttcact ctaagattga tccacatttt 3301 tactgtaagc agaaacttag gacccaagat tataatgctg cagaggatgc tgatcgatgt 3361 gttcttcttc ctgttcctct ttgcggtgtg gatggtggcc tttggcgtgg ccaggcaagg 3421 gatccttagg cagaatgagc agcgctggag gtggatattc cgttcggtca tctacgagcc 3481 ctacctggcc atgttcggcc aggtgcccag tgacgtggat ggtaccacgt atgactttgc 3541 ccactgcacc ttcactggga atgagtccaa gccactgtgt gtggagctgg atgagcacaa 3601 cctgccccgg ttccccgagt ggatcaccat ccccctggtg tgcatctaca tgttatccac 3661 caacatcctg ctggtcaacc tgctggtcgc catgtttggc tacacggtgg gcaccgtcca 3721 ggagaacaat gaccaggtct ggaagttcca gaggtacttc ctggtgcagg agtactgcag 3781 ccgcctcaat atccccttcc ccttcatcgt cttcgcttac ttctacatgg tggtgaagaa 3841 gtgcttcaag tgttgctgca aggagaaaaa catggagtct tctgtctgct gtttcaaaaa 3901 tgaagacaat gagactctgg catgggaggg tgtcatgaag gaaaactacc ttgtcaagat 3961 caacacaaaa gccaacgaca cctcagagga aatgaggcat cgatttagac aactggatac 4021 aaagcttaat gatctcaagg gtcttctgaa agagattgct aataaaatca aaCTCGAGGA 4081 TTACAAGGAT GACGACGATA AGGACTATAA GGACGATGAT GACAAGGACT ACAAAGATGA 4141 TGACGATAAA TGAGATAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT 4201 TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA 4261 TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT GGTTGCTGTC 4321 TCTTTATGAG GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA CTGTGTTTGC 4381 TGACGCAACC CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT CCGGGACTTT 4441 CGCTTTCCCC CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG CCCGCTGCTG 4501 GACAGGGGCT CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGTCGGGGA AGCTGACGTC 4561 CTTTCCATGG CTGCTCGCCT GTGTTGCCAC CTGGATTCTG CGCGGGACGT CCTTCTGCTA 4621 CGTCCCTTCG GCCCTCAATC CAGCGGACCT TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG 4681 GCCTCTTCCG CGTCTTCGCC TTCGCCCTCA GACGAGTCGG ATCTCCCTTT GGGCCGCCTC 4741 CCCGCCTGGT CGAGAAGTAC TAGAGGATCA TAATCAGCCA TACCACATTT GTAGAGGTTT 4801 TACTTGCTTT AAAAAACCTC CCACACCTCC CCCTGAACCT GAAACATAAA ATGAATGCAA 4861 TTGTTGTTGT TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA 4921 CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA 4981 TCAATGTATC TTATCATGTC TGGATCTGAT CAGTAACAAC ACCATTTAAA TGGAGTGGTT 5041 ACAAATGGAG TGGTTAATTA AGTTCCGGAG TATACGTAGC CAACCACTAG AACTATAGCT 5101 AGAGTCCTGG GCGAACAAAC GATGCTCGCC TTCCAGAAAA CCGAGGATGC GAACCACTTC 5161 ATCCGGGGTC AGCACCACCG GCAAGCGCCG CGACGGCCGA GGTCTTCCGA TCTCCTGAAG 5221 CCAGGGCAGA TCCGTGCACA GCACCTTGCC GTAGAAGAAC AGCAAGGCCG CCAATGCCTG 5281 ACGATGCGTG GAGACCGAAA CCTTGCGCTC GTTCGCCAGC CAGGACAGAA ATGCCTCGAC 5341 TTCGCTGCTG CCCAAGGTTG CCGGGTGACG CACACCGTGG AAACGGATGA AGGCACGAAC 5401 CCAGTTGACA TAAGCCTGTT CGGTTCGTAA ACTGTAATGC AAGTAGCGTA TGCGCTCACG 5461 CAACTGGTCC AGAACCTTGA CCGAACGCAG CGGTGGTAAC GGCGCAGTGG CGGTTTTCAT 5521 GGCTTGTTAT GACTGTTTTT TTGTACAGTC TATGCCTCGG GCATCCAAGC AGCAAGCGCG 5581 TTACGCCGTG GGTCGATGTT TGATGTTATG GAGCAGCAAC GATGTTACGC AGCAGGGCAG 5641 TCGCCCTAAA ACAAAGTTAG GTGGCTCAAG TATGGGCATC ATTCGCACAT GTAGGCTCGG 5701 CCCTGACCAA GTCAAATCCA TGCGGGCTGC TCTTGATCTT TTCGGTCGTG AGTTCGGAGA 5761 CGTAGCCACC TACTCCCAAC ATCAGCCGGA CTCCGATTAC CTCGGGAACT TGCTCCGTAG 5821 TAAGACATTC ATCGCGCTTG CTGCCTTCGA CCAAGAAGCG GTTGTTGGCG CTCTCGCGGC 5881 TTACGTTCTG CCCAAGTTTG AGCAGCCGCG TAGTGAGATC TATATCTATG ATCTCGCAGT 5941 CTCCGGCGAG CACCGGAGGC AGGGCATTGC CACCGCGCTC ATCAATCTCC TCAAGCATGA 6001 GGCCAACGCG CTTGGTGCTT ATGTGATCTA CGTGCAAGCA GATTACGGTG ACGATCCCGC 6061 AGTGGCTCTC TATACAAAGT TGGGCATACG GGAAGAAGTG ATGCACTTTG ATATCGACCC 6121 AAGTACCGCC ACCTAACAAT TCGTTCAAGC CGAGATCGGC TTCCCGGCCG CGGAGTTGTT 6181 CGGTAAATTG TCACAACGCC GCGAATATAG TCTTTACCAT GCCCTTGGCC ACGCCCCTCT 6241 TTAATACGAC GGGCAATTTG CACTTCAGAA AATGAAGAGT TTGCTTTAGC CATAACAAAA 6301 GTCCAGTATG CTTTTTCACA GCATAACTGG ACTGATTTCA GTTTACAACT ATTCTGTCTA 6361 GTTTAAGACT TTATTGTCAT AGTTTAGATC TATTTTGTTC AGTTTAAGAC TTTATTGTCC 6421 GCCCACACCC GCTTACGCAG GGCATCCATT TATTACTCAA CCGTAACCGA TTTTGCCAGG 6481 TTACGCGGCT GGTCTATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC 6541 ATCAGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG 6601 CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC 6661 GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG 6721 TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA 6781 AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC 6841 TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC 6901 CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG 6961 GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC 7021 TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA 7081 GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG 7141 AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG 7201 AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT 7261 GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA 7321 GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA 7381 GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA 7441 TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC 7501 TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA 7561 CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA 7621 ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC 7681 GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT 7741 TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC 7801 ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT 7861 TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC 7921 TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG 7981 GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT 8041 GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG 8101 GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA 8161 AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG 8221 TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG 8281 TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT 8341 TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 8401 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 8461 TTTCCCCGAA AAGTGCCACC TAAATTGTAA GCGTTAATAT TTTGTTAAAA TTCGCGTTAA 8521 ATTTTTGTTA AATCAGCTCA TTTTTTAACC AATAGGCCGA AATCGGCAAA ATCCCTTATA 8581 AATCAAAAGA ATAGACCGAG ATAGGGTTGA GTGTTGTTCC AGTTTGGAAC AAGAGTCCAC 8641 TATTAAGAAC GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC 8701 ACTACGTGAA CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA AAGCACTAAA 8761 TCGGAACCCT AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC 8821 GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT 8881 CACGCTGCGC GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCCCA 8941 TTCGCCATTC AGGCTGCAAA TAAGCGTTGA TATTCAGTCA ATTACAAACA TTAATAACGA 9001 AGAGATGACA GAAAAATTTT CATTCTGTGA CAGAGAAAAA GTAGCCGAAG ATGACGGTTT 9061 GTCACATGGA GTTGGCAGGA TGTTTGATTA AAAACATAAC AGGAAGAAAA ATGCCCCGCT 9121 GTGGGCGGAC AAAATAGTTG GGAACTGGGA GGGGTGGAAA TGGAGTTTTT AAGGATTATT 9181 TAGGGAAGAG TGACAAAATA GATGGGAACT GGGTGTAGCG TCGTAAGCTA ATACGAAAAT 9241 TAAAAATGAC AAAATAGTTT GGAACTAGAT TTCACTTATC TGGTTCGGAT CTCCTAGGCT 9301 CAAGCAGTGA TCAGTGGCTA TGGCAGGGCT TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 9361 GCCTTCACCC GAACTTGGGG GTTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGTC 9421 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 9481 ATGAACAAAC GACCCAACAC CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC 9541 GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCAT CTCCCGCTCT 9601 AGTACTTCTC GACAAGCTTT TAGGCCAGGG CTTTACCCTC GGTCTTGGAG GTGACGTCCA 9661 CCTGGACACC CACGAACTTG GACACGCGAC CGTCGGGAGT CTTGATGGGG GTCACAGTCA 9721 GCAGGTTCCA GAAAGGGGTA CCGTCCTTGC GGTAGTTCAG CAGACGCACG GAGCAGGCTT 9781 CACCCTTCTT GATAGCGTCG CGGATCTTCT GCACCTCCTT GGGGTCGGTA CCTTCACCCT 9841 GCAGGAAGCG GGCGTTGTGG CCCAGCACTT CGTCGGGGCC GTAACCAGTC ATGGCGTAGA 9901 AGCCCTCGGA AGCGTACACC AGGGGGCAGT CGGGCAGAGT AGCGTCAGCC ACCACGAAGG 9961 TGTGACGCAG ACCGGCACCG CCACCTTTAC CCAGGCGGTT CATCTCGATG TCAGTATATA 10021 TCTGACGCTT CTTGGTGTGC TTCAGCTTGA TGCACAGGTG GATACCCACG CGCAGGACCA 10081 GGAACAGACC GATGATCAGG CCGATGATGA AGAAGAAAGA AGCGATGCTG GACTTCCAGC 10141 TGCTGAACCA GCCTTCGACC AGTTCGATAG GGTTCTTGCT CAGACCGGTG TCACCAGCGA 10201 AGGCGGAGTG AGCAGCAGCA GCCAGCAGCA CGTACAGCAC GATAGCGCTC ACCATCTTGC 10261 TAGTGTGCTC CTTGTTGAAA CCCTGGTGGC TCTGGTTCAC CAGCAGCATA GATCCGCGCC 10321 CGATGGTGGG ACGGTATGAA TAATCCGGAA TATTTATAGG TTTTTTTATT ACAAAACTGT 10381 TACGAAAACA GTAAAATACT TATTTATTTG CGAGATGGTT ATCATTTTAA TTATCTCCAT 10441 GATCTATTAA TACTTAATTA ACAACACCAT TTGTT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.