pLV3-CMV-Camkk2-3×FLAG-CopGFP-

pLV3-CMV-Camkk2-3×FLAG-CopGFP-Puro plasmid

PVT95567

pLV3-CMV-Camkk2-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Camkk2(mouse)-3×FLAG-CopGFP-Puro
Alias: NM_001199676.1;6330570N16Rik; AW061083; mKIAA0787
Gene lenght: 1764bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: EF1a-R
Copy number: High
Induction:

pLV3-CMV-Camkk2-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Camkk2-3×FLAG-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-Camkk2-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatgt catcatgtgt ctctagccag 2341 cccaccagcg accgggtggc cccccaggat gagctgggaa gtgggggtgg cagccgggaa 2401 ggccagaagc cctgtgaggc actgcgggga ctctcatcct taagtatcca cttgggcatg 2461 gaatccttca tcgtggtcac cgagtgtgag ccaggccggg gtgtggacct caacctggcc 2521 agagaccagc ctccggaggc cgatggccag gaactccccc tcgaagcctc ggaccctgag 2581 tcccggtccc cgctttctgg tcgcaagatg tccctgcagg agccgtccca gggcgggccg 2641 gcatccagca gcaacagcct ggacatgaat ggacgctgca tctgcccatc cctgtcctac 2701 tcaccagcca gctccccaca gtcctctccc cggatgcccc ggcggcccac agtagagtca 2761 caccacgtct ccattaccgg tttgcaggac tgcgtgcagc tgaatcagta caccctgaag 2821 gatgaaattg gaaagggctc ctatggtgtt gtcaagctgg cctacaatga aaatgacaat 2881 acttattacg caatgaaagt gctgtccaaa aagaagctga tccgacaggc tggcttccca 2941 cgtcgccccc caccacgagg agctcgccca gccccagggg gctgcatcca gcccaggggc 3001 cccatcgagc aagtgtacca ggagattgct atcctcaaaa agctggatca tcccaacgtg 3061 gtgaagctgg tagaggtcct ggatgaccct aatgaggacc atctgtacat ggtgtttgag 3121 ttggtgaacc aagggcctgt aatggaagtg cccaccctca agccactgtc cgaagaccag 3181 gcccgcttct acttccagga tctgatcaaa ggcatagagt acttgcatta ccagaagatc 3241 atccatcggg acatcaaacc ttccaacctc ctggtggggg aagacgggca catcaagatt 3301 gccgacttcg gtgtaagcaa cgagttcaag ggcagcgacg ccttgctgtc taacaccgtg 3361 ggcacacctg ccttcatggc acccgagtcg ctctcagaga cccgcaagat cttctccgga 3421 aaggcgttgg atgtttgggc catgggtgtg acgctgtact gctttgtctt tggccagtgc 3481 cctttcatgg atgaacgaat catgtgtttg cacagtaaga tcaagagtca ggccctggag 3541 tttcccgacc agcccgatat agccgaagac ttgaaagatc tgatcactcg gatgttggac 3601 aaaaatccag agtccaggat tgtggtgcct gaaatcaagc tgcacccttg ggttacgagg 3661 cacggggccg agccattgcc gtcggaggac gagaactgca cactggtcga ggtgaccgaa 3721 gaggaggtcg agaattcagt caaacacatt cccagcctgg caactgtgat cctggtaaag 3781 accatgattc gaaagcgctc attcgggaac ccgttcgaag gtagccgccg ggaggaacgt 3841 tccctgtcag cgcctggaaa cctgctcacc aaaaaaccaa ccagggagtg ggagcccttg 3901 tctgagccca aggaagcaag gcagcgaaga cagcccccgg ggcccagagc cggcccctgt 3961 gggggaggag gaagtgctct tgtgaaaggt ggtccctgcg tggaaagttg gggggctccg 4021 gcccctggct ccccaccacg catgcctcca ctgcagcccg aggaggtgat ggagccggag 4081 ggatccGACT ACAAAGACCA TGACGGTGAT TATAAAGATC ATGACATCGA CTACAAGGAT 4141 GACGATGACA AGtagcggcc gcGaaggatc tgcgatcgct ccggtgcccg tcagtgggca 4201 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt 4261 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt 4321 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt 4381 cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca 4441 cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct 4501 cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg 4561 agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg 4621 ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt 4681 tacagatcca agctgtgacc ggcgcctacg ctagacgcca ccatggagag cgacgagagc 4741 ggcctgcccg ccatggagat cgagtgccgc atcaccggca ccctgaacgg cgtggagttc 4801 gagctggtgg gcggcggaga gggcaccccc aagcagggcc gcatgaccaa caagatgaag 4861 agcaccaaag gcgccctgac cttcagcccc tacctgctga gccacgtgat gggctacggc 4921 ttctaccact tcggcaccta ccccagcggc tacgagaacc ccttcctgca cgccatcaac 4981 aacggcggct acaccaacac ccgcatcgag aagtacgagg acggcggcgt gctgcacgtg 5041 agcttcagct accgctacga ggccggccgc gtgatcggcg acttcaaggt ggtgggcacc 5101 ggcttccccg aggacagcgt gatcttcacc gacaagatca tccgcagcaa cgccaccgtg 5161 gagcacctgc accccatggg cgataacgtg ctggtgggca gcttcgcccg caccttcagc 5221 ctgcgcgacg gcggctacta cagcttcgtg gtggacagcc acatgcactt caagagcgcc 5281 atccacccca gcatcctgca gaacgggggc cccatgttcg ccttccgccg cgtggaggag 5341 ctgcacagca acaccgagct gggcatcgtg gagtaccagc acgccttcaa gacccccatc 5401 gccttcgcca gatcccgcgc tcagtcgtcc aattctgccg tggacggcac cgccggaccc 5461 ggctccaccg gatctcgcga gggcagagga agtcttctaa catgcggtga cgtggaggag 5521 aatcccggcc ctatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc 5581 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc 5641 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc 5701 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg 5761 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc 5821 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac 5881 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc 5941 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg 6001 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc 6061 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag 6121 cccggtgcct gaaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt 6181 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct 6241 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt 6301 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac 6361 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct 6421 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca 6481 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaATC ATCGTCCTTT 6541 CCTtggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc 6601 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct 6661 cttccgcgtc tTcgccttcg ccctcagacg agtcggatct ccctttggGc cgcctccccg 6721 cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa 6781 agaaaagggg ggactggaag ggctaattca ctcccaacga aGataagatc tgctttttgc 6841 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg 6901 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg 6961 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat 7021 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg 7081 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa 7141 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 7201 caaactcatc aatgtatctt atcatgtctg gctctagcta TCCCGCCCCT AACTCCGCCC 7261 Atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 7321 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga 7381 ggcttttttg gaggcctaga cttttgcaga gaccaaattc gtaatcatgt catagctgtt 7441 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa 7501 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact 7561 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc 7621 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 7681 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 7741 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 7801 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 7861 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 7921 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 7981 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 8041 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 8101 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 8161 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 8221 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt 8281 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 8341 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 8401 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 8461 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 8521 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 8581 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 8641 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 8701 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 8761 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 8821 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 8881 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 8941 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 9001 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 9061 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 9121 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 9181 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 9241 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 9301 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 9361 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 9421 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 9481 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 9541 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 9601 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt 9661 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct 9721 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg 9781 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg 9841 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt 9901 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct 9961 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc 10021 acgacgttgt aaaacgacgg ccagtgccaa gctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.